Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0177
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g28790.1 68416.m03593 expressed protein 36 0.033
At3g18810.1 68416.m02389 protein kinase family protein contains ... 33 0.13
At4g39380.1 68417.m05574 expressed protein 31 0.71
At4g34300.1 68417.m04875 glycine-rich protein similar to auxin r... 30 1.6
At1g70740.1 68414.m08154 protein kinase family protein contains ... 30 1.6
At5g62940.1 68418.m07897 Dof-type zinc finger domain-containing ... 29 2.8
At3g29080.1 68416.m03641 hypothetical protein 29 2.8
At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi do... 29 2.8
At5g50920.1 68418.m06315 ATP-dependent Clp protease ATP-binding ... 29 3.8
At3g43920.1 68416.m04701 ribonuclease III family protein similar... 29 3.8
At2g43010.2 68415.m05338 phytochrome-interacting factor 4 (PIF4)... 29 3.8
At2g43010.1 68415.m05337 phytochrome-interacting factor 4 (PIF4)... 29 3.8
At2g27660.1 68415.m03352 DC1 domain-containing protein contains ... 29 3.8
At1g07730.2 68414.m00834 disease resistance-responsive family pr... 29 3.8
At3g50690.1 68416.m05546 leucine-rich repeat family protein 28 6.6
At3g48870.1 68416.m05338 ATP-dependent Clp protease ATP-binding ... 28 6.6
At2g16230.1 68415.m01860 glycosyl hydrolase family 17 protein si... 28 6.6
At4g34480.1 68417.m04902 glycosyl hydrolase family 17 protein si... 27 8.7
At3g48470.1 68416.m05291 expressed protein 27 8.7
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 35.5 bits (78), Expect = 0.033
Identities = 34/135 (25%), Positives = 55/135 (40%), Gaps = 2/135 (1%)
Frame = -3
Query: 450 DRLGDGAIDNNHGLSNDFGFVNDNHGFRDNGIDTGSGDNRGRASLVDVQNDLHERSAVSN 271
++ G ++++GL D VN + G + S DN +S Q + + S
Sbjct: 172 EKRGKSIDESSYGLDVD---VNASIGSSSGSDGSSSSDNES-SSNTKSQGTSSKSGSEST 227
Query: 270 GGKRRVSTIAGPTEIGTNSSTMAGPTEI--GSNSLTMAGPTEMGSNSSMMAGPTAIGTKS 97
G +T TE G+ SS+ A E+ GS+ T T S +S PT +
Sbjct: 228 AGSIETNT-GSKTEAGSKSSSSAKTKEVSGGSSGNTYKDTTGSSSGASPSGSPTPTPSTP 286
Query: 96 SMMGAVPATPGLTRP 52
+ P+TP + P
Sbjct: 287 TPSTPTPSTPTPSTP 301
>At3g18810.1 68416.m02389 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 700
Score = 33.5 bits (73), Expect = 0.13
Identities = 27/122 (22%), Positives = 53/122 (43%)
Frame = -3
Query: 474 NGSNVSNLDRLGDGAIDNNHGLSNDFGFVNDNHGFRDNGIDTGSGDNRGRASLVDVQNDL 295
N +N +N + G+ DNN+G + D N+N+G +NG + DN G + +
Sbjct: 79 NNNNDNNNNNNGNNNNDNNNGNNKD----NNNNGNNNNGNNNNGNDNNGNNN----NGNN 130
Query: 294 HERSAVSNGGKRRVSTIAGPTEIGTNSSTMAGPTEIGSNSLTMAGPTEMGSNSSMMAGPT 115
++ + +NGG + P+ ++ ++ + P + + G G+N A
Sbjct: 131 NDNNNQNNGGGSNNRSPPPPSR-NSDRNSPSPPRALAPPRSSGGGSNSSGNNEPNTAAIV 189
Query: 114 AI 109
I
Sbjct: 190 GI 191
>At4g39380.1 68417.m05574 expressed protein
Length = 518
Score = 31.1 bits (67), Expect = 0.71
Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = -2
Query: 199 PNGDRFEFVDNGRSDGNGLELIY--DGRSDSDRYKVVDDGSSSGNAGTHQALSRAGDSN 29
P+ + E +N +DG+G L++ DG + ++ + S GN G H S AG++N
Sbjct: 238 PHDGQVESTENKINDGSGPTLLFWDDGLTSLSIGGLLSEVSLKGNFGNHCKNSNAGNAN 296
>At4g34300.1 68417.m04875 glycine-rich protein similar to auxin
response factor 30 (GI:20145855) {Arabidopsis thaliana}
Length = 313
Score = 29.9 bits (64), Expect = 1.6
Identities = 36/155 (23%), Positives = 58/155 (37%)
Frame = -3
Query: 534 SGRVTAGAGLWESRRSLAWSNGSNVSNLDRLGDGAIDNNHGLSNDFGFVNDNHGFRDNGI 355
SG + G G W GS+ + G G+ N H ++ + G NG
Sbjct: 61 SGGSSWGWGWSSDGTDTNWGWGSSSGSNHSSGTGSTHNGHSSGSNH---SSGTGSTHNGH 117
Query: 354 DTGSGDNRGRASLVDVQNDLHERSAVSNGGKRRVSTIAGPTEIGTNSSTMAGPTEIGSNS 175
+GS + S + + S++ + + G+N S++AGPT G +S
Sbjct: 118 SSGSNHSSSTGSTHNNHSSGSNHSSIVGSTHKNHGS-------GSNHSSIAGPTHNGHSS 170
Query: 174 LTMAGPTEMGSNSSMMAGPTAIGTKSSMMGAVPAT 70
GSN S + G T T S+ + T
Sbjct: 171 ---------GSNHSSIIGSTHNHTAPSLGRKIAVT 196
>At1g70740.1 68414.m08154 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 425
Score = 29.9 bits (64), Expect = 1.6
Identities = 17/57 (29%), Positives = 27/57 (47%)
Frame = -3
Query: 264 KRRVSTIAGPTEIGTNSSTMAGPTEIGSNSLTMAGPTEMGSNSSMMAGPTAIGTKSS 94
+RR +G +GT S+T + GSN T G G+ +S A + T+S+
Sbjct: 353 RRRTQRPSGAASLGTLSTTGSSTDSFGSNLNTNTGTGVRGTPASSKASTRSNATRSA 409
>At5g62940.1 68418.m07897 Dof-type zinc finger domain-containing
protein Dof zinc finger protein, Oryza sativa,
EMBL:AB028129
Length = 372
Score = 29.1 bits (62), Expect = 2.8
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = -3
Query: 441 GDGAIDNNH--GLSNDFGFVNDNHGFRDNGIDTGSGDNRGRASLVDV 307
GD +NH GL++ G N+N+ NGI TG N G L+D+
Sbjct: 233 GDHHHHHNHHMGLNHGVGLNNNNNNGGFNGISTGGNGNGG--GLMDI 277
>At3g29080.1 68416.m03641 hypothetical protein
Length = 445
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -3
Query: 249 TIAGPTEIGTNSSTMAGPTEIGSNSLTMAGPTEMGSNS 136
T +G TEIG ++ ++ +GS + T PT GS +
Sbjct: 321 TASGQTEIGVSTRCISTSVVVGSEATTNTEPTTTGSRT 358
>At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi
domain-containing protein contains Pfam profiles
PF02170: PAZ domain, PF02171: Piwi domain
Length = 1194
Score = 29.1 bits (62), Expect = 2.8
Identities = 25/77 (32%), Positives = 30/77 (38%), Gaps = 2/77 (2%)
Frame = -2
Query: 241 GSNGDRYELVDDGRPNGDRFEFVDNGRSDGNGLELIYDGRSDSDR-YKVVDDGSSSGNAG 65
G G Y DGR G + +GR DG+G G D R Y DG G G
Sbjct: 20 GDRGRGYSGRGDGRGRGGDGDRGYSGRGDGHG----RGGGGDRGRGYSGRGDGRGRGGGG 75
Query: 64 TH-QALSRAGDSNGHHG 17
+ S GD +G G
Sbjct: 76 DRGRGYSGRGDGHGRGG 92
>At5g50920.1 68418.m06315 ATP-dependent Clp protease ATP-binding
subunit / ClpC almost identical to ClpC GI:2921158 from
[Arabidopsis thaliana]; contains Pfam profile PF02861:
Clp amino terminal domain; contains Pfam profile
PF00004: ATPase, AAA family; contains Pfam profile
PF02151: UvrB/uvrC motif
Length = 929
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +2
Query: 116 VGPAIIDEFEP-ISVGPAIVNEFEPISVGPAIVDEFVPISVG 238
+G +DE+ I PA+ F+P+ V VDE + I G
Sbjct: 406 IGATTLDEYRKHIEKDPALERRFQPVKVPEPTVDETIQILKG 447
>At3g43920.1 68416.m04701 ribonuclease III family protein similar to
RNA helicase/RNAseIII CAF protein [Arabidopsis thaliana]
GI:6102610; contains Pfam profiles PF02170: PAZ domain,
PF00636: RNase3 domain
Length = 1531
Score = 28.7 bits (61), Expect = 3.8
Identities = 41/175 (23%), Positives = 65/175 (37%), Gaps = 2/175 (1%)
Frame = -3
Query: 558 LIDIQLRVSGRVTAGAGLWESRRSLAWSNGSNVSNLDRLGDGAIDNNHGLSNDFGFVNDN 379
LI+++ ++ +A L E+ + + L+ LGD +D F+
Sbjct: 1149 LIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLD----------FLITR 1198
Query: 378 HGFRDNGIDTGSGDNRGRASLVDVQNDLHERSAVSNGGKRRVSTIAGPTEIGTNSSTMA- 202
H F TG G+ S V N+ + AV N + A E N M+
Sbjct: 1199 HLFNTYE-QTGPGEMTDLRSAC-VNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSF 1256
Query: 201 -GPTEIGSNSLTMAGPTEMGSNSSMMAGPTAIGTKSSMMGAVPATPGLTRPLVGP 40
P E G + ++ GP +G +AG I T+ + L PLV P
Sbjct: 1257 QKPDETGRSIPSIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTP 1311
>At2g43010.2 68415.m05338 phytochrome-interacting factor 4 (PIF4) /
basic helix-loop-helix protein 9 (bHLH9) / short under
red-light 2 (SRL2) identical to SP|Q8W2F3
Phytochrome-interacting factor 4 (Basic helix-loop-helix
protein 9) (bHLH9) (Short under red-light 2)
{Arabidopsis thaliana}; supporting cDNA
gi|18026965|gb|AF251694.1|AF251694
Length = 428
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = -3
Query: 222 TNSSTMAGPTEIGSNSLTMAGPTEMGSNSS 133
TNSS+ TE+ S+T GP+ GSN S
Sbjct: 142 TNSSSGIRETEMEQYSVTTVGPSHCGSNPS 171
>At2g43010.1 68415.m05337 phytochrome-interacting factor 4 (PIF4) /
basic helix-loop-helix protein 9 (bHLH9) / short under
red-light 2 (SRL2) identical to SP|Q8W2F3
Phytochrome-interacting factor 4 (Basic helix-loop-helix
protein 9) (bHLH9) (Short under red-light 2)
{Arabidopsis thaliana}; supporting cDNA
gi|18026965|gb|AF251694.1|AF251694
Length = 430
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = -3
Query: 222 TNSSTMAGPTEIGSNSLTMAGPTEMGSNSS 133
TNSS+ TE+ S+T GP+ GSN S
Sbjct: 142 TNSSSGIRETEMEQYSVTTVGPSHCGSNPS 171
>At2g27660.1 68415.m03352 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 718
Score = 28.7 bits (61), Expect = 3.8
Identities = 20/53 (37%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = -2
Query: 232 GDRYELVDDGRPNGDRFEFVDNGRS-DGNGLELIYDGRSDSDRYKVVDDGSSS 77
GD Y V++ G+ + D G DGNGLE++ + S Y D GSSS
Sbjct: 310 GDVY--VEEANDEGEDVDGNDEGEDVDGNGLEIVACVDNLSVAYSESDFGSSS 360
>At1g07730.2 68414.m00834 disease resistance-responsive family
protein contains Pfam PF03018: Plant disease resistance
response protein
Length = 389
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/67 (25%), Positives = 33/67 (49%)
Frame = -3
Query: 276 SNGGKRRVSTIAGPTEIGTNSSTMAGPTEIGSNSLTMAGPTEMGSNSSMMAGPTAIGTKS 97
++GG + T GP + T S++ P S +L + GP + ++S ++ G ++
Sbjct: 70 ASGGLGSLGTNTGPGPLSTTGSSLL-PV-ASSGTLPVTGPGPLPTSSGLLPGASSGNLPG 127
Query: 96 SMMGAVP 76
S G +P
Sbjct: 128 SGSGPLP 134
>At3g50690.1 68416.m05546 leucine-rich repeat family protein
Length = 447
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/58 (20%), Positives = 28/58 (48%)
Frame = -2
Query: 175 VDNGRSDGNGLELIYDGRSDSDRYKVVDDGSSSGNAGTHQALSRAGDSNGHHGSEGQE 2
+++ ++ +G++ D D + +V + G +G+ L AG+ +GH + E
Sbjct: 283 IEDSENEEDGVDDEEDDEEDEEEEEVDNADRGLGGSGSTSRLMNAGEIDGHEQGDDDE 340
>At3g48870.1 68416.m05338 ATP-dependent Clp protease ATP-binding
subunit (ClpC) identical to AtClpC GI:5360574 from
[Arabidopsis thaliana]; contains Pfam profiles PF02861:
Clp amino terminal domain and PF02151: UvrB/uvrC motif
Length = 952
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +2
Query: 116 VGPAIIDEFEP-ISVGPAIVNEFEPISVGPAIVDEFVPISVG 238
+G IDE+ I PA+ F+P+ V V+E + I G
Sbjct: 427 IGATTIDEYRKHIEKDPALERRFQPVKVPEPTVEEAIQILQG 468
>At2g16230.1 68415.m01860 glycosyl hydrolase family 17 protein
similar to elicitor inducible chitinase Nt-SubE76
GI:11071974 from [Nicotiana tabacum]
Length = 456
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = -3
Query: 309 VQNDLHERSAVSNGGKRRVSTIAGPTEIGTNSSTMAG 199
+QN AVS GGK +VST+ T +G + AG
Sbjct: 135 MQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAG 171
>At4g34480.1 68417.m04902 glycosyl hydrolase family 17 protein
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]
Length = 356
Score = 27.5 bits (58), Expect = 8.7
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -3
Query: 309 VQNDLHERSAVSNGGKRRVSTIAGPTEIGTNSSTMAGPTEIG 184
+QN AVS GGK +VST+ T +G++ +G G
Sbjct: 136 MQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAG 177
>At3g48470.1 68416.m05291 expressed protein
Length = 1017
Score = 27.5 bits (58), Expect = 8.7
Identities = 14/48 (29%), Positives = 22/48 (45%), Gaps = 2/48 (4%)
Frame = -3
Query: 363 NGIDTGSGDNRGRASLVDVQNDLHERSAVSNGGKRRVS--TIAGPTEI 226
N ++ G G+N+ ASL +V N + +S +A P EI
Sbjct: 524 NTMENGDGENKRSASLTEVNESSRRNKQKENRKSKNISAFVLADPNEI 571
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,109,021
Number of Sequences: 28952
Number of extensions: 255799
Number of successful extensions: 1028
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 938
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1013
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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