Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0176
         (829 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC191.11 |inv1||beta-fructofuranosidase|Schizosaccharomyces po...    50   4e-07
SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo...    49   7e-07
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma...    27   3.2  
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch...    27   4.3  
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|...    27   4.3  
SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   5.7  
SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosacch...    26   5.7  
SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm...    26   5.7  
SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom...    26   7.5  
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ...    26   7.5  
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    25   9.9  
SPBC1778.01c |zuo1|mpp11, SPBC30D10.01|zuotin |Schizosaccharomyc...    25   9.9  
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||...    25   9.9  

>SPCC191.11 |inv1||beta-fructofuranosidase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 581

 Score = 50.0 bits (114), Expect = 4e-07
 Identities = 34/95 (35%), Positives = 47/95 (49%), Gaps = 16/95 (16%)
 Frame = +3

Query: 3   HWGHVSSSNLIDWEHLPTALIPE------TEMCFSGGAVVHGDDLVLLYTG--------- 137
           HWGH  S +LI WE+ P A+ P+        + FSG AVV   +   L++          
Sbjct: 125 HWGHTVSKDLIHWENYPIAIYPDEHENGVLSLPFSGSAVVDVHNSSGLFSNDTIPEERIV 184

Query: 138 RVTTDTDPFYNETQYLAFSND-GVNFRKYEGNPVL 239
            + TD      E Q +A++ D G  F+KY GNPVL
Sbjct: 185 LIYTDHWTGVAERQAIAYTTDGGYTFKKYSGNPVL 219



 Score = 47.2 bits (107), Expect = 3e-06
 Identities = 27/65 (41%), Positives = 37/65 (56%), Gaps = 1/65 (1%)
 Frame = +2

Query: 254 HSADFRDPK-IWKFKDHWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMW 430
           +S  FRDPK IW F  + +V+I + S   G +  Y S DL +W  LSV   S G +G  +
Sbjct: 222 NSLQFRDPKVIWDFDANRWVMIVAMSQNYG-IAFYSSYDLIHWTELSVFSTS-GYLGLQY 279

Query: 431 ECPDL 445
           ECP +
Sbjct: 280 ECPGM 284


>SPAC8E11.01c
           ||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 508

 Score = 49.2 bits (112), Expect = 7e-07
 Identities = 32/87 (36%), Positives = 47/87 (54%), Gaps = 3/87 (3%)
 Frame = +2

Query: 227 KSSPLLRARHSADFRDPKI-WKFKDHWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGE 403
           K +P+L  + S  FRDPK+ W  +   ++++   + K  +VL Y S +L +W  LS  G 
Sbjct: 136 KKNPILDIKES-QFRDPKVFWHEESRAWIMVVVLAQKY-KVLFYHSLNLRDWVKLSEFG- 192

Query: 404 SDGDMGYMWECPDLFEL--GGKTIFLW 478
           S G +GY +ECPD   L   G   F W
Sbjct: 193 SAGVLGYQYECPDFVRLPIEGTDEFRW 219



 Score = 48.8 bits (111), Expect = 9e-07
 Identities = 36/96 (37%), Positives = 49/96 (51%), Gaps = 17/96 (17%)
 Frame = +3

Query: 3   HWGHVSSSNLIDWEHLPTALIPETE--MCFSGGAVVHGDDLVLLYT----GRVTTD---- 152
           HWGH  S NL  W+ LPTAL P  +  + FSG AV+   +    +      R + D    
Sbjct: 46  HWGHAVSKNLYKWKLLPTALAPGDDHGLMFSGSAVIDKTNSSGFFESGFFSRKSVDPEER 105

Query: 153 -----TDPFYN-ETQYLAFSND-GVNFRKYEGNPVL 239
                T  + N ETQ +A+S D G+ F KY+ NP+L
Sbjct: 106 IVLIYTTHYDNRETQNIAYSLDGGITFIKYKKNPIL 141



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 29/78 (37%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
 Frame = +1

Query: 535 YYIGELDYETFEFKTDKYFQELDYGHDFYATQTIQG--DGKTYLIGWFNMWEVPH-LEKE 705
           Y+IG+ D +TF    D   + LD GHD YATQT     DG+   I W + W   + +   
Sbjct: 239 YFIGDFDGQTFT-PIDSASRILDCGHDCYATQTFGNAPDGRVISISWASNWNYTNDVPMR 297

Query: 706 DGWAGTTTLVRELQLIGT 759
               G  T+ REL L  T
Sbjct: 298 MKHRGMFTIPRELTLCYT 315


>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 318

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = +3

Query: 27  NLIDWEHLPTALIPETEMCFSGGAVVHGD 113
           N++    + T L+  T++C SGG  +HG+
Sbjct: 3   NIVVLGSMNTDLVMRTKICPSGGETIHGE 31


>SPAP8A3.14c |||mitochondrial inner membrane protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 677

 Score = 26.6 bits (56), Expect = 4.3
 Identities = 12/46 (26%), Positives = 22/46 (47%)
 Frame = +1

Query: 109 EMTWYCYTRAASLLTRTRFTTKLNI*PSVTTELTSASMKEIQSSPT 246
           E +W     +   LTR   T  + I PS+  +++S  +K++    T
Sbjct: 344 EYSWNTSVLSDEALTRNSLTLPVPIKPSINKDISSIEVKDLSQKNT 389


>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1369

 Score = 26.6 bits (56), Expect = 4.3
 Identities = 12/44 (27%), Positives = 22/44 (50%)
 Frame = +3

Query: 51   PTALIPETEMCFSGGAVVHGDDLVLLYTGRVTTDTDPFYNETQY 182
            P  L+PE      G  +V  DD+ LL    ++ +T P ++ + +
Sbjct: 1166 PDHLVPEPSRPHCGNGLVFYDDIPLLEVNPISGETIPKFDASSF 1209


>SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 773

 Score = 26.2 bits (55), Expect = 5.7
 Identities = 11/34 (32%), Positives = 16/34 (47%)
 Frame = +2

Query: 407 DGDMGYMWECPDLFELGGKTIFLWSPQGLEPKGD 508
           +G+  Y+W C  L   G   I LW    LE + +
Sbjct: 83  EGNWHYIWTCTSLKSNGEWKILLWKFNDLEEESE 116


>SPAC2G11.03c |vps45||vacuolar sorting protein Vps
           45|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 558

 Score = 26.2 bits (55), Expect = 5.7
 Identities = 10/22 (45%), Positives = 14/22 (63%)
 Frame = +3

Query: 156 DPFYNETQYLAFSNDGVNFRKY 221
           DPFY ET++  F + GV  + Y
Sbjct: 269 DPFYKETRFDNFGDLGVKIKDY 290


>SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1292

 Score = 26.2 bits (55), Expect = 5.7
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = -2

Query: 750  QLQLSNERRRPRPAVLFLKVRHFPHIKPANQVSLAVAL 637
            Q++   ER RPR    F+K+R  P +  A  V+L + +
Sbjct: 1223 QMEALPERLRPRVKQRFMKIRS-PSVSSATSVALMIPI 1259


>SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1125

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 5/48 (10%)
 Frame = -1

Query: 568  QKFHSLIPQ-----YSIRSDTCSCIVAFRFQSLRGPEENGFSAEFKQI 440
            QKF SL PQ     Y +RS       +F  + +R  ++N +++  KQ+
Sbjct: 1052 QKFLSLSPQVSKDYYDVRSKLNDTAGSFSGEEMRAIDDNYYASRIKQL 1099


>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1337

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 12/31 (38%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
 Frame = +1

Query: 586 YFQEL-DYGHDFYATQTIQGDGKTYLIGWFN 675
           YFQ L D+    Y  +TIQ  G+  +  W N
Sbjct: 507 YFQRLIDHNMQIYDDETIQKAGEATVSSWLN 537


>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 25.4 bits (53), Expect = 9.9
 Identities = 13/42 (30%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
 Frame = +3

Query: 162 FYNETQYLAFSNDGVNF-RKYEGNPVLSYVPDIQLISETPRF 284
           +++E+  + F N    F RK   +P+  + PD Q +  TP F
Sbjct: 298 WFSESAMILFLNKLDLFKRKVHISPIQKHFPDYQEVGSTPTF 339


>SPBC1778.01c |zuo1|mpp11, SPBC30D10.01|zuotin |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 442

 Score = 25.4 bits (53), Expect = 9.9
 Identities = 11/18 (61%), Positives = 13/18 (72%)
 Frame = +2

Query: 272 DPKIWKFKDHWYVVIGSS 325
           DPK WK +DH Y V+G S
Sbjct: 89  DPKEWKQQDH-YAVLGLS 105


>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 3971

 Score = 25.4 bits (53), Expect = 9.9
 Identities = 22/79 (27%), Positives = 39/79 (49%)
 Frame = +1

Query: 19   PVAT*STGNTSLRLSSLKPKCASRAAPSSTEMTWYCYTRAASLLTRTRFTTKLNI*PSVT 198
            P+ + +T NTS  ++S     +S A  SST +       ++S+L  +   T  +I  S +
Sbjct: 3299 PITSSTTLNTSTPITSSSVLNSSTAITSSTALNTSTPITSSSVLNSSTAITSSSILNS-S 3357

Query: 199  TELTSASMKEIQSSPTCPT 255
            T +TS+S+    +  T  T
Sbjct: 3358 TPVTSSSVLNSSTPITSST 3376


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,413,655
Number of Sequences: 5004
Number of extensions: 72636
Number of successful extensions: 207
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 406444570
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)