Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0176
(829 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g55120.1 68414.m06295 beta-fructosidase, putative / beta-fruc... 54 9e-08
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 52 5e-07
At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) / beta-fruc... 51 1e-06
At5g11920.1 68418.m01394 glycosyl hydrolase family 32 protein si... 50 2e-06
At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc... 48 6e-06
At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) / beta-fruc... 46 4e-05
At3g13784.1 68416.m01741 beta-fructosidase, putative / beta-fruc... 44 2e-04
At3g52600.1 68416.m05794 beta-fructosidase, putative / beta-fruc... 42 7e-04
At1g26860.1 68414.m03276 hypothetical protein 28 6.6
At1g12230.1 68414.m01415 transaldolase, putative similar to Swis... 28 6.6
At4g17570.1 68417.m02627 zinc finger (GATA type) family protein 28 8.7
At3g19410.1 68416.m02462 F-box family protein contains Pfam PF00... 28 8.7
>At1g55120.1 68414.m06295 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:402740
Length = 594
Score = 54.4 bits (125), Expect = 9e-08
Identities = 29/64 (45%), Positives = 39/64 (60%), Gaps = 3/64 (4%)
Frame = +2
Query: 266 FRDPKI-WKFKD-HWYVVIGSSSN-KRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWEC 436
FRDP W +D W V++GSS++ +RG +LY+S D FNW S+ D+ MWEC
Sbjct: 178 FRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWT-QSMKPLHYEDLTGMWEC 236
Query: 437 PDLF 448
PD F
Sbjct: 237 PDFF 240
Score = 32.3 bits (70), Expect = 0.40
Identities = 26/80 (32%), Positives = 36/80 (45%), Gaps = 8/80 (10%)
Frame = +1
Query: 535 YYIGELDYETFEFKTDKYFQE------LDYGHDFYATQTIQGDGKTYLI--GWFNMWEVP 690
Y IG D E + D F + LDYG +YA++T D K I GW N
Sbjct: 276 YTIGSYDREKDVYVPDLGFVQNESAPRLDYGK-YYASKTFYDDVKKRRILWGWVNESSPA 334
Query: 691 HLEKEDGWAGTTTLVRELQL 750
+ E GW+G + R++ L
Sbjct: 335 KDDIEKGWSGLQSFPRKIWL 354
Score = 31.9 bits (69), Expect = 0.53
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 5/49 (10%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIP----ETEMCFSGG-AVVHGDDLVLLYTG 137
WGH +S +L++W P A P + C+SG ++ V+LYTG
Sbjct: 83 WGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTG 131
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 52.0 bits (119), Expect = 5e-07
Identities = 28/63 (44%), Positives = 32/63 (50%), Gaps = 2/63 (3%)
Frame = +2
Query: 266 FRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWECP 439
FRDP W KD HW V+GS +RG +YRS D +W S G MWECP
Sbjct: 186 FRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTG-MWECP 244
Query: 440 DLF 448
D F
Sbjct: 245 DFF 247
Score = 31.5 bits (68), Expect = 0.71
Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 5/49 (10%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIPETEMCFSGG-----AVVHGDDLVLLYTG 137
W H S +L++WE L AL P G +V G ++LYTG
Sbjct: 93 WAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTG 141
>At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) /
beta-fructofuranosidase / cell wall invertase identical
to beta-fructofuranosidase GI:402740 from [Arabidopsis
thaliana]
Length = 584
Score = 50.8 bits (116), Expect = 1e-06
Identities = 27/67 (40%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +2
Query: 254 HSADFRDPKI-WKFKDH-WYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYM 427
+++ FRDP W +D W V+IGS ++RG + Y S D WE D G M
Sbjct: 186 NASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSG-M 244
Query: 428 WECPDLF 448
WECPD F
Sbjct: 245 WECPDFF 251
Score = 36.3 bits (80), Expect = 0.025
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 5/49 (10%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIPETEM----CFSGGA-VVHGDDLVLLYTG 137
W H +S++LI+W+ P A+ P C+SG A ++ V+LYTG
Sbjct: 95 WAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTG 143
Score = 33.5 bits (73), Expect = 0.18
Identities = 21/62 (33%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
Frame = +1
Query: 571 FKTDKYFQELDYGHDFYATQTIQGDGKTYLI--GWFNMWEVPHLEKEDGWAGTTTLVREL 744
FK D DYG +YA++T K I GW N + E GW+G T+ R++
Sbjct: 307 FKMDGTAPRYDYGK-YYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKI 365
Query: 745 QL 750
L
Sbjct: 366 WL 367
>At5g11920.1 68418.m01394 glycosyl hydrolase family 32 protein
similar to fructan 1-exohydrolase IIa GI:13940209 from
[Cichorium intybus]; contains Pfam profile PF00251:
Glycosyl hydrolases family 32
Length = 550
Score = 50.0 bits (114), Expect = 2e-06
Identities = 30/66 (45%), Positives = 38/66 (57%), Gaps = 5/66 (7%)
Frame = +2
Query: 266 FRDP-KIWKFKD-HWYVVIGSSSN--KRGRVLLYRSGDLFNWEFLSV-LGESDGDMGYMW 430
FRDP + WK +D W V+IG+ ++G +LYRS D W V L ES+G MW
Sbjct: 156 FRDPTEAWKGQDGKWRVLIGAKEKDTEKGMAILYRSDDFVQWTKYPVPLLESEGTG--MW 213
Query: 431 ECPDLF 448
ECPD F
Sbjct: 214 ECPDFF 219
Score = 38.3 bits (85), Expect = 0.006
Identities = 19/49 (38%), Positives = 28/49 (57%), Gaps = 5/49 (10%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIP----ETEMCFSGGAVVHGDDL-VLLYTG 137
WGH S ++++W L AL+P + C+SG A + D V+LYTG
Sbjct: 62 WGHSVSQDMVNWIQLEPALVPSESFDINSCWSGSATILPDGRPVILYTG 110
Score = 38.3 bits (85), Expect = 0.006
Identities = 29/79 (36%), Positives = 36/79 (45%), Gaps = 9/79 (11%)
Frame = +1
Query: 535 YYIGELDYETFEFKTDKYFQ----ELDYGHD-FYATQTIQGDGKTYLIGWFNMWEVPHLE 699
Y IG+ ET +F D F +L Y H FYA++ K I W W +
Sbjct: 252 YVIGKYSSETEDFSADYEFTNTSADLRYDHGTFYASKAFFDSVKNRRINWG--WVIETDS 309
Query: 700 KED----GWAGTTTLVREL 744
KED GWAG TL RE+
Sbjct: 310 KEDDFKKGWAGLMTLPREI 328
>At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis
thaliana]; supported by full-length cDNA GI:14517549;
identical to cDNA Beta-fructosidase GI:3115854
Length = 648
Score = 48.4 bits (110), Expect = 6e-06
Identities = 35/94 (37%), Positives = 49/94 (52%), Gaps = 10/94 (10%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIPETEMCFSG---GAVVHGDD--LVLLYTGRVTTDTDPFYN 170
WGH S +LI W +LP A++P+ +G G+ DD +V+LYTG TD F
Sbjct: 149 WGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTG----STDEFV- 203
Query: 171 ETQYLAFSNDG-----VNFRKYEGNPVLSYVPDI 257
+ Q LA+ D + + K+ GNPVL P I
Sbjct: 204 QVQNLAYPEDPSDPLLLKWVKFSGNPVLVPPPGI 237
Score = 41.1 bits (92), Expect = 9e-04
Identities = 24/64 (37%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +2
Query: 263 DFRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWEC 436
DFRDP WK W + IGS N+ G L+Y + D +E L + G MWEC
Sbjct: 241 DFRDPTTAWKTSSGKWRITIGSKINRTGISLIYDTTDFKTYEKHETLLHQVPNTG-MWEC 299
Query: 437 PDLF 448
D +
Sbjct: 300 VDFY 303
>At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GI:1871503 from [Arabidopsis
thaliana]; contains Pfam profile PF00251:Glycosyl
hydrolases family 32; identical to cDNA
beta-fructosidase (vacuolar form) GI:1321683; similar to
SP:Q43857
Length = 664
Score = 45.6 bits (103), Expect = 4e-05
Identities = 32/91 (35%), Positives = 45/91 (49%), Gaps = 7/91 (7%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIP----ETEMCFSGGAVVHGD-DLVLLYTGRVTTDTDPFYN 170
WGH S +LI W HLP A++ ++ ++G A D +V+LYTG +TD
Sbjct: 164 WGHAVSRDLIHWVHLPIAMVADQWYDSNGVWTGSATFLPDGSIVMLYTG--STDKAVQVQ 221
Query: 171 ETQYLAFSNDGVNFR--KYEGNPVLSYVPDI 257
Y ND + + K+ GNPVL P I
Sbjct: 222 NLAYPEDPNDPLLLKWVKFPGNPVLVPPPGI 252
Score = 44.4 bits (100), Expect = 9e-05
Identities = 26/64 (40%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = +2
Query: 263 DFRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWEC 436
DFRDP WK + W + IGS NK G L+Y + D +E L L + G MWEC
Sbjct: 256 DFRDPTTAWKTSEGKWRITIGSKLNKTGISLVYDTIDFKTYEKLDTLLHRVPNTG-MWEC 314
Query: 437 PDLF 448
D +
Sbjct: 315 VDFY 318
>At3g13784.1 68416.m01741 beta-fructosidase, putative /
beta-fructofuranosidase, putative / cell wall invertase,
putative similar to beta-fructofuranosidase GI:402740
from [Arabidopsis thaliana]
Length = 569
Score = 43.6 bits (98), Expect = 2e-04
Identities = 26/68 (38%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = +2
Query: 254 HSADFRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNW-EFLSVLGESDGDMGY 424
+S+ FRDP W +D W + GS +RG +L+ S D W + L DG
Sbjct: 176 NSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFVIWKQSPKPLHYHDGT--G 233
Query: 425 MWECPDLF 448
MWECPD F
Sbjct: 234 MWECPDFF 241
Score = 33.9 bits (74), Expect = 0.13
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 5/49 (10%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIP----ETEMCFSGG-AVVHGDDLVLLYTG 137
WGH +S++LI+W L A+ P + C+SG ++ V+LYTG
Sbjct: 85 WGHATSTDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTG 133
>At3g52600.1 68416.m05794 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase [Daucus carota] GI:18324
Length = 590
Score = 41.5 bits (93), Expect = 7e-04
Identities = 25/64 (39%), Positives = 31/64 (48%), Gaps = 3/64 (4%)
Frame = +2
Query: 266 FRDPKIWKF--KD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWEC 436
FRDP F KD +W +++GS RG +Y+S D W S G MWEC
Sbjct: 184 FRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTG-MWEC 242
Query: 437 PDLF 448
PD F
Sbjct: 243 PDFF 246
Score = 33.9 bits (74), Expect = 0.13
Identities = 20/55 (36%), Positives = 26/55 (47%), Gaps = 5/55 (9%)
Frame = +3
Query: 6 WGHVSSSNLIDWEHLPTALIPETEMCFSG---GAVVH--GDDLVLLYTGRVTTDT 155
W H S +LI+WE L A+ P +G G+ H G V+LYTG T
Sbjct: 91 WAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGITENQT 145
Score = 31.1 bits (67), Expect = 0.93
Identities = 22/80 (27%), Positives = 36/80 (45%), Gaps = 8/80 (10%)
Frame = +1
Query: 535 YYIGELDYETFEFKTDKYFQE------LDYGHDFYATQTIQGD--GKTYLIGWFNMWEVP 690
Y +G D + ++ D Y + DYG+ +YA++T D + L GW N +
Sbjct: 282 YTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGN-YYASKTFFDDKTNRRILWGWANESDTV 340
Query: 691 HLEKEDGWAGTTTLVRELQL 750
+ GWAG + R + L
Sbjct: 341 QDDTVKGWAGIQLIPRTILL 360
>At1g26860.1 68414.m03276 hypothetical protein
Length = 240
Score = 28.3 bits (60), Expect = 6.6
Identities = 11/47 (23%), Positives = 26/47 (55%)
Frame = +2
Query: 314 IGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWECPDLFEL 454
+G+++ +R L+ + F W ++G + D+ ++ +C DL E+
Sbjct: 188 LGAANLRRSLSPLHAELEAFLWAMKCMIGADNQDVVFLTDCSDLVEM 234
>At1g12230.1 68414.m01415 transaldolase, putative similar to
Swiss-Prot:P30148 transaldolase B (EC 2.2.1.2)
[Escherichia coli O157:H7]
Length = 405
Score = 28.3 bits (60), Expect = 6.6
Identities = 14/27 (51%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = +3
Query: 81 CFSGGAVVH-GDDLVLLYTGRVTTDTD 158
CF A+V+ G DLV L GRV+T+ D
Sbjct: 141 CFFNKAIVNVGGDLVKLVPGRVSTEVD 167
>At4g17570.1 68417.m02627 zinc finger (GATA type) family protein
Length = 510
Score = 27.9 bits (59), Expect = 8.7
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = +1
Query: 442 SV*TRRKNHFPLVPSRIGTERRRYKNTYQ 528
SV T K+ P VP I T +RRY+N Q
Sbjct: 401 SVTTTSKSSIPNVPKNIVTIKRRYENQIQ 429
>At3g19410.1 68416.m02462 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain; similar to hypothetical
protein GB:AAC69119 from [Arabidopsis thaliana]
Length = 373
Score = 27.9 bits (59), Expect = 8.7
Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 5/54 (9%)
Frame = +1
Query: 514 KNTYQT--GYYIGELDYETFEFKTD--KYFQELDYGHD-FYATQTIQGDGKTYL 660
K TY +Y+ D+E FEF +D ++ ++ G Y+ Q + G TY+
Sbjct: 154 KKTYYKILSFYLDSKDFEIFEFNSDSWRFIDDICPGLSLLYSDQCVSLKGNTYM 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,152,646
Number of Sequences: 28952
Number of extensions: 391205
Number of successful extensions: 1106
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1055
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1101
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1902108000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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