Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0156
(834 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 128 1e-30
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 128 1e-30
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 75 9e-15
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 63 5e-11
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 38 0.002
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 31 0.15
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 31 0.20
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 31 0.27
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 30 0.35
SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ... 28 1.9
SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosa... 28 1.9
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 27 2.5
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 27 2.5
SPBP4G3.02 |pho1||acid phosphatase Pho1 |Schizosaccharomyces pom... 27 4.3
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 27 4.3
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 27 4.3
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 27 4.3
SPBC23E6.09 |ssn6||transcriptional corepressor Ssn6|Schizosaccha... 26 5.7
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 26 7.6
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 128 bits (309), Expect = 1e-30
Identities = 63/81 (77%), Positives = 70/81 (86%)
Frame = +1
Query: 10 MGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKN 189
MG +E IED P+GNI GLVGVDQFLVK+GT+TT + AHNMKVMKFSVSPVV+VAVE KN
Sbjct: 438 MGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKN 497
Query: 190 PADLPKLVEGLKRLAKSDPMV 252
DLPKLVEGLKRL+KSDP V
Sbjct: 498 GNDLPKLVEGLKRLSKSDPCV 518
Score = 126 bits (304), Expect = 4e-30
Identities = 59/85 (69%), Positives = 67/85 (78%)
Frame = +3
Query: 255 CINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKS 434
C ESGEHIVAGAGELHLEICLKDL+EDHA IP+K S PVVSYRE+V+E S LSKS
Sbjct: 520 CTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPSSMTALSKS 579
Query: 435 PNKHNRLFMKAQPMPDGLPEDIDEG 509
PNKHNR+FM A+PM + L I+ G
Sbjct: 580 PNKHNRIFMTAEPMSEELSVAIETG 604
Score = 69.7 bits (163), Expect = 5e-13
Identities = 42/112 (37%), Positives = 57/112 (50%), Gaps = 5/112 (4%)
Frame = +2
Query: 506 GRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTAPTSWWIAPKEFSTSMKLR--- 676
G VNPRDDFK RAR + +++ +DVT+ARKIWCFGP+ T K + +++
Sbjct: 604 GHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSV 663
Query: 677 --TLLWLDSSGPLRKELWLKRICVVLDSTSICNTPY*CHP*SGGQIIPTTRR 826
W GP+ +E +LD + H GGQIIPT RR
Sbjct: 664 VAAFAWASKEGPMFEENLRSCRFNILDVVLHADA---IHR-GGGQIIPTARR 711
Score = 69.3 bits (162), Expect = 6e-13
Identities = 32/45 (71%), Positives = 36/45 (80%)
Frame = +1
Query: 625 NILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNI 759
N++VD +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI
Sbjct: 644 NVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNI 688
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 128 bits (309), Expect = 1e-30
Identities = 63/81 (77%), Positives = 70/81 (86%)
Frame = +1
Query: 10 MGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKN 189
MG +E IED P+GNI GLVGVDQFLVK+GT+TT + AHNMKVMKFSVSPVV+VAVE KN
Sbjct: 438 MGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKN 497
Query: 190 PADLPKLVEGLKRLAKSDPMV 252
DLPKLVEGLKRL+KSDP V
Sbjct: 498 GNDLPKLVEGLKRLSKSDPCV 518
Score = 126 bits (304), Expect = 4e-30
Identities = 59/85 (69%), Positives = 67/85 (78%)
Frame = +3
Query: 255 CINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKS 434
C ESGEHIVAGAGELHLEICLKDL+EDHA IP+K S PVVSYRE+V+E S LSKS
Sbjct: 520 CTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPSSMTALSKS 579
Query: 435 PNKHNRLFMKAQPMPDGLPEDIDEG 509
PNKHNR+FM A+PM + L I+ G
Sbjct: 580 PNKHNRIFMTAEPMSEELSVAIETG 604
Score = 69.7 bits (163), Expect = 5e-13
Identities = 42/112 (37%), Positives = 57/112 (50%), Gaps = 5/112 (4%)
Frame = +2
Query: 506 GRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTAPTSWWIAPKEFSTSMKLR--- 676
G VNPRDDFK RAR + +++ +DVT+ARKIWCFGP+ T K + +++
Sbjct: 604 GHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSV 663
Query: 677 --TLLWLDSSGPLRKELWLKRICVVLDSTSICNTPY*CHP*SGGQIIPTTRR 826
W GP+ +E +LD + H GGQIIPT RR
Sbjct: 664 VAAFAWASKEGPMFEENLRSCRFNILDVVLHADA---IHR-GGGQIIPTARR 711
Score = 69.3 bits (162), Expect = 6e-13
Identities = 32/45 (71%), Positives = 36/45 (80%)
Frame = +1
Query: 625 NILVDCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNI 759
N++VD +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI
Sbjct: 644 NVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNI 688
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 75.4 bits (177), Expect = 9e-15
Identities = 34/82 (41%), Positives = 50/82 (60%)
Frame = +3
Query: 264 EESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNK 443
EESGEH + G GE++++ L DL ++ I I+ SDPV + ET + S C S +PNK
Sbjct: 635 EESGEHTIFGTGEMYMDCLLYDLRTLYSEIEIRVSDPVARFCETAVDTSSIKCFSDTPNK 694
Query: 444 HNRLFMKAQPMPDGLPEDIDEG 509
NR+ M +P+ G+ DI+ G
Sbjct: 695 KNRITMVVEPLEKGISNDIENG 716
Score = 49.2 bits (112), Expect = 7e-07
Identities = 28/81 (34%), Positives = 46/81 (56%), Gaps = 3/81 (3%)
Frame = +1
Query: 16 RYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFK---NAHNMKVMKFSVSPVVRVAVEPK 186
RY ++ +G + L GVD + KT TI + + + + + V +VAVEP
Sbjct: 549 RYRLHVDGAVAGMLVLLGGVDNSISKTATIVSDNLKDDPYIFRPIAHMSESVFKVAVEPH 608
Query: 187 NPADLPKLVEGLKRLAKSDPM 249
NP++LPKL++GL++ KS P+
Sbjct: 609 NPSELPKLLDGLRKTNKSYPL 629
Score = 38.7 bits (86), Expect = 0.001
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +1
Query: 637 DCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNI 759
D K V LN +K+ + GFQW +EG + +E +R V F +
Sbjct: 766 DVDKNV--LNSVKEYIKQGFQWGTREGPLCDETIRNVNFRL 804
Score = 32.3 bits (70), Expect = 0.087
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +2
Query: 506 GRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPE 613
G+VN K + + + Y++D+ +R IW FGP+
Sbjct: 716 GKVNINWPQKRISEFFQKNYDWDLLASRSIWAFGPD 751
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 62.9 bits (146), Expect = 5e-11
Identities = 31/93 (33%), Positives = 57/93 (61%), Gaps = 1/93 (1%)
Frame = +1
Query: 10 MGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKN 189
MG+ + +E VP+GN+ + G+ +++T T+ + N N+ + + P+VRVA+EP
Sbjct: 513 MGQELVYLETVPAGNVFAIGGLAGTVLRTATLCSSPNGPNLVGVTQQMEPIVRVALEPVR 572
Query: 190 PADLPKLVEGLKRLAKSDPMVHVLM-KNQENTL 285
P ++ KLV GL L ++DP V + + +N E+ +
Sbjct: 573 PFEMNKLVTGLDMLNQADPCVQIAVEENGEHVI 605
Score = 62.9 bits (146), Expect = 5e-11
Identities = 30/56 (53%), Positives = 37/56 (66%)
Frame = +3
Query: 264 EESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSK 431
EE+GEH++ AGE+HLE CLKDL E A I I+ S P+V YRET D L +K
Sbjct: 598 EENGEHVIMCAGEIHLERCLKDLRERFAKIEIQASQPLVPYRETTIATPDLLAKNK 653
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 37.9 bits (84), Expect = 0.002
Identities = 16/50 (32%), Positives = 34/50 (68%)
Frame = +3
Query: 258 INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEE 407
++ ES E I++G GELHLE+ ++ + ++ + + P V++RET++++
Sbjct: 508 LDNESKETIISGMGELHLEVYVERMRREYK-VDCETGKPRVAFRETLSKK 556
>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 241
Score = 31.5 bits (68), Expect = 0.15
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 190 PADLPKLVEGLKRLAKSDPMVHVLMKNQENTLSLVLEN 303
P D+ KL EGL++L + D + V M N+ T + + N
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRN 209
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 31.1 bits (67), Expect = 0.20
Identities = 25/82 (30%), Positives = 38/82 (46%), Gaps = 8/82 (9%)
Frame = +1
Query: 25 EAIEDVPSGNICGLVGVDQFLVKTGTIT----TFKNAHNMKVMKFSV----SPVVRVAVE 180
+ ++ + +GNI + G+ QF TG I KN H SV PV ++E
Sbjct: 393 QEVDCISAGNIGVISGIKQF--HTGDIIINKENSKNFHEYLSGNQSVISIPEPVCIASIE 450
Query: 181 PKNPADLPKLVEGLKRLAKSDP 246
P + D P L+E L + + DP
Sbjct: 451 PYSLKDEPALLEALANMNREDP 472
Score = 27.9 bits (59), Expect = 1.9
Identities = 16/61 (26%), Positives = 28/61 (45%)
Frame = +3
Query: 267 ESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKH 446
E+G+ ++ G G +HL++ + L + V YRET+ + S + NK
Sbjct: 481 ENGQLLIQGMGIMHLQVSYERLVSEFGA-RASLGKVQVGYRETLIDVSFNSVTLSTENKE 539
Query: 447 N 449
N
Sbjct: 540 N 540
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 30.7 bits (66), Expect = 0.27
Identities = 23/82 (28%), Positives = 39/82 (47%)
Frame = +3
Query: 243 PHGACINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLC 422
P+ +EE+ + A E C++DL++ + K+SD Y T+
Sbjct: 192 PNSVLNDEETLHELFPNASEF---TCVRDLKKLEKKVK-KRSDLGNKYESTLNS-----L 242
Query: 423 LSKSPNKHNRLFMKAQPMPDGL 488
++KS KHN+L K +P+P L
Sbjct: 243 INKSVKKHNKLVKKHKPLPSTL 264
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 30.3 bits (65), Expect = 0.35
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = +3
Query: 321 LKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMP 479
+K LEE+ + +K SD V + ++ + P +++FM PMP
Sbjct: 287 VKYLEENFGALKVKLSDATVKEIREACDNAEVIGARYPPGAGSKIFMDTPPMP 339
>SPAC21E11.06 |tif224||translation initiation factor eIF2B delta
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +1
Query: 67 VGVDQFLVKTGTITTFKNAHNMKV--MKFSVSP 159
+GVD F K G + +K+ N+K+ +K+ V+P
Sbjct: 404 MGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTP 436
>SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 27.9 bits (59), Expect = 1.9
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = -1
Query: 705 GPLESSHNR--VLNFIEVLNSFGAIHQDVGAVPSGPKHQILRASVTSYSYFS 556
G L SSHN V+ + E+ + ++V G KH +LRA+ +Y YFS
Sbjct: 288 GLLVSSHNHPCVVEYSEISDEACKATENV----DGHKHLLLRAANIAYHYFS 335
>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
modification enzyme|Schizosaccharomyces pombe|chr
2|||Manual
Length = 666
Score = 27.5 bits (58), Expect = 2.5
Identities = 13/29 (44%), Positives = 15/29 (51%)
Frame = +2
Query: 581 EARKIWCFGPEGTAPTSWWIAPKEFSTSM 667
+AR + PEG P SWW P S SM
Sbjct: 307 KARHLIWLEPEGLDPNSWWY-PNGLSNSM 334
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 27.5 bits (58), Expect = 2.5
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = +3
Query: 312 EICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNK 443
E+C +LE+D I +S +S + A++ DQL S + +K
Sbjct: 64 ELCNFELEKDSLESMIHESSTALSAQSNTAQDGDQLASSSTISK 107
>SPBP4G3.02 |pho1||acid phosphatase Pho1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 453
Score = 26.6 bits (56), Expect = 4.3
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -1
Query: 786 WHQYGVLHIDVESNTTQILFSHNSFLSGPLESSHN 682
W+ YG+ DV + T IL S N+ SS+N
Sbjct: 171 WYGYGMFGEDVHNFTNYILVSENATAGSNSLSSYN 205
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 26.6 bits (56), Expect = 4.3
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 76 DQFLVKTGTITTFKNAHNMKVMKFSVSPVV 165
D FLVK G + ++ +N+ FSVS +V
Sbjct: 82 DTFLVKYGDLINIQDPYNLNKTLFSVSDLV 111
>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
Ste6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 26.6 bits (56), Expect = 4.3
Identities = 13/64 (20%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = -1
Query: 681 RVLNFIEVLNSFGAIHQDVGAVPSGPKHQILR--ASVTSYSYFSVRYRARVLKSSRGFTR 508
+V +++ LN+F ++ + A+ S P H++ + A++ S + S + ++ + F+
Sbjct: 739 QVCDYLRELNNFASLFSIISALNSSPIHRLRKTWANLNSKTLASFELLNNLTEARKNFSN 798
Query: 507 PHQC 496
C
Sbjct: 799 YRDC 802
>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 230
Score = 26.6 bits (56), Expect = 4.3
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 613 LGAKAPNLTGFGNIIFVLFCKIPSAS 536
+G PNL GN +F L ++PS+S
Sbjct: 169 IGILPPNLNSIGNKMFSLKSRVPSSS 194
>SPBC23E6.09 |ssn6||transcriptional corepressor
Ssn6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1102
Score = 26.2 bits (55), Expect = 5.7
Identities = 10/22 (45%), Positives = 18/22 (81%)
Frame = -2
Query: 488 QTIRHGLSLHEQTVVLVGRL*E 423
+++RH +SL+E+T + +GRL E
Sbjct: 324 ESVRHLISLNEETWIQIGRLAE 345
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 25.8 bits (54), Expect = 7.6
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +3
Query: 495 DIDEGA*IPAMTSRLALGILQKSTNMMLPKPVRFGALAPRV 617
+ID G IP++T R +G L + ++L + ++AP++
Sbjct: 981 EIDTGN-IPSLTLRRQIGFLTMTEELLLQISSKLSSVAPKI 1020
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,700,081
Number of Sequences: 5004
Number of extensions: 80198
Number of successful extensions: 272
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 243
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 267
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 410448950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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