Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0154
         (759 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   122   4e-29
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   120   2e-28
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    27   3.8  

>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  122 bits (295), Expect = 4e-29
 Identities = 58/88 (65%), Positives = 69/88 (78%), Gaps = 1/88 (1%)
 Frame = +3

Query: 3   VLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQDGFLVVNGNKIAVF 179
           VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++DG LV++GN I V 
Sbjct: 18  VLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKDGKLVIDGNAIDVH 77

Query: 180 SERDPKAIPWGKAGAEYVVESTGVFPLQ 263
           +ERDP  I W  +GA+YV+ESTGVF  Q
Sbjct: 78  NERDPADIKWSTSGADYVIESTGVFTTQ 105



 Score =  116 bits (280), Expect = 3e-27
 Identities = 56/73 (76%), Positives = 64/73 (87%)
 Frame = +2

Query: 254 STTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAK 433
           +T + ASAHL+GGAK+VIISAPS DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAK
Sbjct: 103 TTQETASAHLKGGAKRVIISAPSKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAK 162

Query: 434 VIHDNFEIVEGLM 472
           VI+D F I EGLM
Sbjct: 163 VINDTFGIEEGLM 175



 Score =  116 bits (280), Expect = 3e-27
 Identities = 56/72 (77%), Positives = 61/72 (84%)
 Frame = +1

Query: 511 DGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVR 690
           DGPS K WR GRGA  NIIP+STGAAKAVGKVIPALNGKLTGMAFRVP  +VSVVDLTV+
Sbjct: 189 DGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDVSVVDLTVK 248

Query: 691 LGKPASYESHQA 726
           L KP +YE  +A
Sbjct: 249 LAKPTNYEDIKA 260


>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  120 bits (290), Expect = 2e-28
 Identities = 58/73 (79%), Positives = 65/73 (89%)
 Frame = +2

Query: 254 STTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAK 433
           +T + ASAHL+GGAK+VIISAPS DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAK
Sbjct: 103 TTKETASAHLKGGAKRVIISAPSKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAK 162

Query: 434 VIHDNFEIVEGLM 472
           VI+D F I EGLM
Sbjct: 163 VINDTFGIEEGLM 175



 Score =  118 bits (284), Expect = 9e-28
 Identities = 56/85 (65%), Positives = 67/85 (78%), Gaps = 1/85 (1%)
 Frame = +3

Query: 3   VLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQDGFLVVNGNKIAVF 179
           VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + G LV++G+ I V 
Sbjct: 18  VLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKGGKLVIDGHSIDVH 77

Query: 180 SERDPKAIPWGKAGAEYVVESTGVF 254
           +ERDP  I W  +GAEYV+ESTGVF
Sbjct: 78  NERDPANIKWSASGAEYVIESTGVF 102



 Score =  116 bits (280), Expect = 3e-27
 Identities = 56/72 (77%), Positives = 61/72 (84%)
 Frame = +1

Query: 511 DGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVR 690
           DGPS K WR GRGA  NIIP+STGAAKAVGKVIPALNGKLTGMAFRVP  +VSVVDLTV+
Sbjct: 189 DGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDVSVVDLTVK 248

Query: 691 LGKPASYESHQA 726
           L KP +YE  +A
Sbjct: 249 LAKPTNYEDIKA 260


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
 Frame = -2

Query: 170 NFVTVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 15
           +F  + N+ESIL+L   L +     + E I++IV+   +D R     HLS L  +
Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,113,941
Number of Sequences: 5004
Number of extensions: 65275
Number of successful extensions: 182
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)