Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0154
(759 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 118 3e-27
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 117 1e-26
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 113 9e-26
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 113 9e-26
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 85 5e-17
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 85 6e-17
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 78 7e-15
At5g43030.1 68418.m05250 DC1 domain-containing protein contains ... 30 1.9
At5g43040.1 68418.m05254 DC1 domain-containing protein contains ... 29 4.4
At5g25020.1 68418.m02965 expressed protein 28 7.7
At2g44030.1 68415.m05474 kelch repeat-containing F-box family pr... 28 7.7
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 118 bits (285), Expect = 3e-27
Identities = 53/73 (72%), Positives = 63/73 (86%)
Frame = +2
Query: 254 STTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAK 433
+T KA++HL+GGAKKVIISAPSADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAK
Sbjct: 187 TTLSKAASHLKGGAKKVIISAPSADAPMFVVGVNEHTYQPNMDIVSNASCTTNCLAPLAK 246
Query: 434 VIHDNFEIVEGLM 472
V+H+ F I+EGLM
Sbjct: 247 VVHEEFGILEGLM 259
Score = 116 bits (279), Expect = 2e-26
Identities = 57/72 (79%), Positives = 61/72 (84%)
Frame = +1
Query: 511 DGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVR 690
DGPS K WR GRGA QNIIP+STGAAKAVGKV+P LNGKLTGMAFRVP +NVSVVDLT R
Sbjct: 273 DGPSMKDWRGGRGASQNIIPSSTGAAKAVGKVLPELNGKLTGMAFRVPTSNVSVVDLTCR 332
Query: 691 LGKPASYESHQA 726
L K ASYE +A
Sbjct: 333 LEKGASYEDVKA 344
Score = 105 bits (253), Expect = 2e-23
Identities = 54/108 (50%), Positives = 67/108 (62%), Gaps = 2/108 (1%)
Frame = +3
Query: 3 VLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV-QDGFLVVNGNKIAV 176
VLR A+ +VVA+NDPFI YM Y+ KYDSTHG FKGS+ V D L +NG K+ V
Sbjct: 101 VLRIATSRDDIEVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVIDDSTLEINGKKVNV 160
Query: 177 FSERDPKAIPWGKAGAEYVVESTGVFPLQIKHLLTWREVLKKLLYQLP 320
S+RDP IPW GA+YVVES+GVF K + KK++ P
Sbjct: 161 VSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKGGAKKVIISAP 208
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 117 bits (281), Expect = 1e-26
Identities = 53/73 (72%), Positives = 64/73 (87%)
Frame = +2
Query: 254 STTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAK 433
+T +AS+HL+GGAKKVIISAPSADAPMFVVGVN + Y P+ ++SNASCTTNCLAPLAK
Sbjct: 185 TTVGQASSHLKGGAKKVIISAPSADAPMFVVGVNEKTYLPNMDIVSNASCTTNCLAPLAK 244
Query: 434 VIHDNFEIVEGLM 472
V+H+ F I+EGLM
Sbjct: 245 VVHEEFGILEGLM 257
Score = 116 bits (278), Expect = 2e-26
Identities = 57/72 (79%), Positives = 60/72 (83%)
Frame = +1
Query: 511 DGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVR 690
DGPS K WR GRGA QNIIP+STGAAKAVGKV+P LNGKLTGMAFRVP NVSVVDLT R
Sbjct: 271 DGPSMKDWRGGRGASQNIIPSSTGAAKAVGKVLPELNGKLTGMAFRVPTPNVSVVDLTCR 330
Query: 691 LGKPASYESHQA 726
L K ASYE +A
Sbjct: 331 LEKDASYEDVKA 342
Score = 104 bits (250), Expect = 6e-23
Identities = 49/86 (56%), Positives = 61/86 (70%), Gaps = 2/86 (2%)
Frame = +3
Query: 3 VLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV-QDGFLVVNGNKIAV 176
VLR A+ +VVA+NDPFI YM Y+FKYDSTHG +KG++ V D L +NG ++ V
Sbjct: 99 VLRIATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVIDDSTLEINGKQVKV 158
Query: 177 FSERDPKAIPWGKAGAEYVVESTGVF 254
S+RDP IPW GAEYVVES+GVF
Sbjct: 159 VSKRDPAEIPWADLGAEYVVESSGVF 184
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 113 bits (273), Expect = 9e-26
Identities = 53/70 (75%), Positives = 59/70 (84%)
Frame = +2
Query: 263 DKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIH 442
DKA+AHL+GGAKKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+
Sbjct: 110 DKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVIN 169
Query: 443 DNFEIVEGLM 472
D F IVEGLM
Sbjct: 170 DRFGIVEGLM 179
Score = 108 bits (259), Expect = 5e-24
Identities = 52/68 (76%), Positives = 58/68 (85%)
Frame = +1
Query: 511 DGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVR 690
DGPS K WR GR A NIIP+STGAAKAVGKV+PALNGKLTGM+FRVP +VSVVDLTVR
Sbjct: 193 DGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTVDVSVVDLTVR 252
Query: 691 LGKPASYE 714
L K A+Y+
Sbjct: 253 LEKAATYD 260
Score = 90.6 bits (215), Expect = 1e-18
Identities = 44/98 (44%), Positives = 66/98 (67%), Gaps = 2/98 (2%)
Frame = +3
Query: 33 QVVAINDPFIGLDYMVYLFKYDSTHGRFK-GSVEVQDGFLVVNGNK-IAVFSERDPKAIP 206
++VA+NDPFI +YM Y+FKYDS HG++K ++++D ++ G K + VF R+P+ IP
Sbjct: 31 ELVAVNDPFITTEYMTYMFKYDSVHGQWKHNELKIKDEKTLLFGEKPVTVFGIRNPEDIP 90
Query: 207 WGKAGAEYVVESTGVFPLQIKHLLTWREVLKKLLYQLP 320
W +AGA+YVVESTGVF + K + KK++ P
Sbjct: 91 WAEAGADYVVESTGVFTDKDKAAAHLKGGAKKVVISAP 128
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 113 bits (273), Expect = 9e-26
Identities = 53/70 (75%), Positives = 59/70 (84%)
Frame = +2
Query: 263 DKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIH 442
DKA+AHL+GGAKKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+
Sbjct: 110 DKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVIN 169
Query: 443 DNFEIVEGLM 472
D F IVEGLM
Sbjct: 170 DRFGIVEGLM 179
Score = 107 bits (256), Expect = 1e-23
Identities = 51/68 (75%), Positives = 58/68 (85%)
Frame = +1
Query: 511 DGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVR 690
DGPS K WR GR A NIIP+STGAAKAVGKV+P+LNGKLTGM+FRVP +VSVVDLTVR
Sbjct: 193 DGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPSLNGKLTGMSFRVPTVDVSVVDLTVR 252
Query: 691 LGKPASYE 714
L K A+Y+
Sbjct: 253 LEKAATYD 260
Score = 91.5 bits (217), Expect = 6e-19
Identities = 45/98 (45%), Positives = 67/98 (68%), Gaps = 2/98 (2%)
Frame = +3
Query: 33 QVVAINDPFIGLDYMVYLFKYDSTHGRFKG-SVEVQDGFLVVNGNK-IAVFSERDPKAIP 206
++VA+NDPFI +YM Y+FKYDS HG++K ++V+D ++ G K + VF R+P+ IP
Sbjct: 31 ELVAVNDPFITTEYMTYMFKYDSVHGQWKHHELKVKDDKTLLFGEKPVTVFGIRNPEDIP 90
Query: 207 WGKAGAEYVVESTGVFPLQIKHLLTWREVLKKLLYQLP 320
WG+AGA++VVESTGVF + K + KK++ P
Sbjct: 91 WGEAGADFVVESTGVFTDKDKAAAHLKGGAKKVVISAP 128
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 85.0 bits (201), Expect = 5e-17
Identities = 40/69 (57%), Positives = 48/69 (69%), Gaps = 1/69 (1%)
Frame = +2
Query: 269 ASAHLEGGAKKVIISAPS-ADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHD 445
A H+E GAKKVII+AP D P +VVGVN +AY +ISNASCTTNCLAP KV+
Sbjct: 168 AGKHIEAGAKKVIITAPGKGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKVLDQ 227
Query: 446 NFEIVEGLM 472
F I++G M
Sbjct: 228 KFGIIKGTM 236
Score = 69.7 bits (163), Expect = 2e-12
Identities = 36/64 (56%), Positives = 41/64 (64%)
Frame = +1
Query: 535 RDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVRLGKPASYE 714
R R A NI+P STGAAKAV V+P L GKL G+A RVP NVSVVDL V++ K E
Sbjct: 257 RRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLVVQVSKKTFAE 316
Query: 715 SHQA 726
A
Sbjct: 317 EVNA 320
Score = 55.6 bits (128), Expect = 3e-08
Identities = 30/74 (40%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
Frame = +3
Query: 36 VVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQ-DGFLVVNGNKIAVFSERDPKAIPWG 212
++AIND G+ +L KYDST G F V+ + + V+G I V S R+P +PW
Sbjct: 90 IIAINDTG-GVKQASHLLKYDSTLGIFDADVKPSGETAISVDGKIIQVVSNRNPSLLPWK 148
Query: 213 KAGAEYVVESTGVF 254
+ G + V+E TGVF
Sbjct: 149 ELGIDIVIEGTGVF 162
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 84.6 bits (200), Expect = 6e-17
Identities = 40/71 (56%), Positives = 48/71 (67%), Gaps = 1/71 (1%)
Frame = +2
Query: 263 DKASAHLEGGAKKVIISAPS-ADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVI 439
D A HL+ GAKKV+I+AP D P +VVGVN E Y +ISNASCTTNCLAP KV+
Sbjct: 169 DGAGKHLQAGAKKVLITAPGKGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKVL 228
Query: 440 HDNFEIVEGLM 472
F I++G M
Sbjct: 229 DQKFGIIKGTM 239
Score = 69.7 bits (163), Expect = 2e-12
Identities = 36/64 (56%), Positives = 41/64 (64%)
Frame = +1
Query: 535 RDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVRLGKPASYE 714
R R A NI+P STGAAKAV V+P L GKL G+A RVP NVSVVDL V++ K E
Sbjct: 260 RRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLVVQVSKKTFAE 319
Query: 715 SHQA 726
A
Sbjct: 320 EVNA 323
Score = 61.3 bits (142), Expect = 7e-10
Identities = 33/74 (44%), Positives = 44/74 (59%), Gaps = 1/74 (1%)
Frame = +3
Query: 36 VVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQ-DGFLVVNGNKIAVFSERDPKAIPWG 212
VV IND G+ +L KYDST G F V+ D L V+G I + S+R+P +PWG
Sbjct: 93 VVVINDTG-GVKQASHLLKYDSTLGIFDADVKPSGDSALSVDGKIIKIVSDRNPSNLPWG 151
Query: 213 KAGAEYVVESTGVF 254
+ G + V+E TGVF
Sbjct: 152 ELGIDLVIEGTGVF 165
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 77.8 bits (183), Expect = 7e-15
Identities = 39/71 (54%), Positives = 50/71 (70%), Gaps = 3/71 (4%)
Frame = +2
Query: 269 ASAHLEGGAKKVIISAPS--ADAPMFVVGVNLEAYDPSF-KVISNASCTTNCLAPLAKVI 439
A H++ GA KVII+AP+ AD P +V+GVN + Y +ISNASCTTNCLAP AKV+
Sbjct: 188 AGKHIQAGASKVIITAPAKGADIPTYVMGVNEQDYGHDVANIISNASCTTNCLAPFAKVL 247
Query: 440 HDNFEIVEGLM 472
+ F IV+G M
Sbjct: 248 DEEFGIVKGTM 258
Score = 68.1 bits (159), Expect = 6e-12
Identities = 33/55 (60%), Positives = 38/55 (69%)
Frame = +1
Query: 535 RDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVSVVDLTVRLGK 699
R R A NI+P STGAAKAV V+P L GKL G+A RVP NVSVVDL + + K
Sbjct: 279 RRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLVINVEK 333
Score = 61.7 bits (143), Expect = 5e-10
Identities = 32/75 (42%), Positives = 44/75 (58%), Gaps = 1/75 (1%)
Frame = +3
Query: 33 QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQDGFLV-VNGNKIAVFSERDPKAIPW 209
+VV +ND G+ +L KYDS G FK V++ D + V+G I V S RDP +PW
Sbjct: 109 EVVVLNDSG-GVKNASHLLKYDSMLGTFKAEVKIVDNETISVDGKLIKVVSNRDPLKLPW 167
Query: 210 GKAGAEYVVESTGVF 254
+ G + V+E TGVF
Sbjct: 168 AELGIDIVIEGTGVF 182
>At5g43030.1 68418.m05250 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 564
Score = 29.9 bits (64), Expect = 1.9
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -3
Query: 547 HGHHAIIFQKVHQSFLCSSCGMNSSHQAL 461
H H +IF K SF C +CG+N ++ L
Sbjct: 117 HEHPLLIFLKGSFSFPCDACGVNDANSCL 145
>At5g43040.1 68418.m05254 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 551
Score = 28.7 bits (61), Expect = 4.4
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -3
Query: 547 HGHHAIIFQKVHQSFLCSSCGMNSSHQAL 461
H H ++F K SF C +CG+N + L
Sbjct: 122 HEHPLLVFLKGSFSFPCDACGVNDAKSCL 150
>At5g25020.1 68418.m02965 expressed protein
Length = 269
Score = 27.9 bits (59), Expect = 7.7
Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
Frame = +2
Query: 239 VYWCLSTTDKASAHLEGGAKKVIISAPSADAPMFVVGVNL-----EAYDPSFKVISN 394
V W STT K L +VI + ++ + F+ VN E Y+ FK++SN
Sbjct: 86 VDWLKSTTKKLDHILSRPDNRVIHALKTSQSSSFIFAVNFQVPGKEHYNLRFKIVSN 142
>At2g44030.1 68415.m05474 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 380
Score = 27.9 bits (59), Expect = 7.7
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +1
Query: 1 WCSVLLLKRELKWSL*MTLSSVLTI 75
WC+++LL+REL W + +V T+
Sbjct: 332 WCALILLERELVWGVIEWSENVFTL 356
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,626,088
Number of Sequences: 28952
Number of extensions: 354853
Number of successful extensions: 971
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 911
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 961
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -