BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0147 (708 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g24560.1 68417.m03520 ubiquitin-specific protease 16, putativ... 29 3.0 At4g16230.1 68417.m02463 GDSL-motif lipase/hydrolase family prot... 28 5.3 At5g47430.1 68418.m05844 expressed protein 28 7.0 At3g62700.1 68416.m07043 glutathione-conjugate transporter, puta... 28 7.0 At1g18080.1 68414.m02238 WD-40 repeat family protein / auxin-dep... 27 9.2 >At4g24560.1 68417.m03520 ubiquitin-specific protease 16, putative (UBP16) similar to ubiquitin-specific protease 16 GI:11993477 [Arabidopsis thaliana] Length = 1008 Score = 29.1 bits (62), Expect = 3.0 Identities = 15/36 (41%), Positives = 21/36 (58%), Gaps = 3/36 (8%) Frame = +2 Query: 284 KPDDDANTNPSKSRASQNLPPDR---KCDPLRRSGE 382 KP+DDA N S+SR+ +L DR DP +S + Sbjct: 278 KPEDDAGQNQSQSRSLHSLVTDRHPVSADPSLKSSD 313 >At4g16230.1 68417.m02463 GDSL-motif lipase/hydrolase family protein similar to SP|P40602 Anther-specific proline-rich protein APG precursor {Arabidopsis thaliana}; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase Length = 340 Score = 28.3 bits (60), Expect = 5.3 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +2 Query: 356 CDPLRRSGEKLSGHQSNCEITPSLIALVYNFRTYVL 463 C P R + ++G++ C + P+ +A +YN + +L Sbjct: 197 CIPFERESDPMAGYE--CSVEPNEVAQMYNLKLKIL 230 >At5g47430.1 68418.m05844 expressed protein Length = 879 Score = 27.9 bits (59), Expect = 7.0 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 5/53 (9%) Frame = +2 Query: 266 GELTGLKPDDDANTN-----PSKSRASQNLPPDRKCDPLRRSGEKLSGHQSNC 409 G + LKP++DA PS +R+ LPP+ KC PL + K + S C Sbjct: 260 GAVAVLKPNEDAFEKEMEGLPSTTRSVGELPPELKC-PLCKEVMKDAALTSKC 311 >At3g62700.1 68416.m07043 glutathione-conjugate transporter, putative similar to glutathione-conjugate transporter AtMRP4 GI:2959767 from [Arabidopsis thaliana] Length = 1539 Score = 27.9 bits (59), Expect = 7.0 Identities = 13/30 (43%), Positives = 15/30 (50%) Frame = +1 Query: 316 QEPCFAESTTGSEMRPTEKIRRETQWAPVE 405 QEP E T S + PTEK E W +E Sbjct: 1379 QEPVLFEGTVRSNIDPTEKYSDEEIWKSLE 1408 >At1g18080.1 68414.m02238 WD-40 repeat family protein / auxin-dependent protein (ARCA) / guanine nucleotide-binding protein beta subunit, putative identical to SP|O24456 Guanine nucleotide-binding protein beta subunit-like protein (WD-40 repeat auxin-dependent protein ARCA) {Arabidopsis thaliana}; contains 7 WD-40 repeats (PF00400) Length = 327 Score = 27.5 bits (58), Expect = 9.2 Identities = 13/41 (31%), Positives = 23/41 (56%) Frame = -2 Query: 593 HSYKIDNILASSDSVIRLSNIIYGKTRLTQMLQSVAIRLFL 471 HS+ +++++ SSD LS G+ RL + V+ R F+ Sbjct: 62 HSHFVEDVVLSSDGQFALSGSWDGELRLWDLAAGVSTRRFV 102 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,067,007 Number of Sequences: 28952 Number of extensions: 229812 Number of successful extensions: 496 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 491 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 496 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1526202912 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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