Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0139
         (699 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Sch...   147   2e-36
SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Sc...   147   2e-36
SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual       29   0.64 
SPBC1105.03c |mrpl16||mitochondrial ribosomal protein subunit L1...    28   1.1  
SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S...    27   2.0  
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ...    27   2.6  
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    27   2.6  
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon...    27   3.4  
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz...    26   4.5  
SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   4.5  
SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccha...    25   7.9  
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|...    25   7.9  
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces...    25   7.9  
SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosacch...    25   7.9  
SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosacchar...    25   7.9  
SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccha...    25   7.9  
SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|...    25   7.9  

>SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein
           L10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 221

 Score =  147 bits (356), Expect = 2e-36
 Identities = 64/78 (82%), Positives = 71/78 (91%)
 Frame = +2

Query: 14  MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193
           M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+
Sbjct: 1   MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60

Query: 194 SSEALEAGRICCNKYLVK 247
           +SEALEA RIC NKYLVK
Sbjct: 61  TSEALEAARICANKYLVK 78



 Score =  136 bits (328), Expect = 4e-33
 Identities = 64/94 (68%), Positives = 75/94 (79%), Gaps = 1/94 (1%)
 Frame = +1

Query: 256 KDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMSVRSS 435
           KD FH+R+R HPFHV+RINKMLSCAGADRLQTGMR AFGKP G VARV IGQ +MSVR+ 
Sbjct: 82  KDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQVLMSVRTK 141

Query: 436 DRWKAQVIEALRRAKFKFPGRQKISYQRS-GVSQ 534
           D  +A  IEALRR ++KFPG+Q+I   +  G SQ
Sbjct: 142 DSSRATAIEALRRCQYKFPGQQRIIVSKKWGFSQ 175



 Score = 53.2 bits (122), Expect = 3e-08
 Identities = 21/52 (40%), Positives = 32/52 (61%)
 Frame = +3

Query: 474 CQVQVPRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629
           CQ + P   + +VSKKWGF++Y RDE+ + R  G +  DGC  ++  + G L
Sbjct: 155 CQYKFPGQQRIIVSKKWGFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206


>SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein
           L10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 221

 Score =  147 bits (356), Expect = 2e-36
 Identities = 64/78 (82%), Positives = 71/78 (91%)
 Frame = +2

Query: 14  MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193
           M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+
Sbjct: 1   MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60

Query: 194 SSEALEAGRICCNKYLVK 247
           +SEALEA RIC NKYLVK
Sbjct: 61  TSEALEAARICANKYLVK 78



 Score =  135 bits (327), Expect = 5e-33
 Identities = 64/94 (68%), Positives = 75/94 (79%), Gaps = 1/94 (1%)
 Frame = +1

Query: 256 KDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMSVRSS 435
           KD FH+R+R HPFHV+RINKMLSCAGADRLQTGMR AFGKP G VARV IGQ +MSVR+ 
Sbjct: 82  KDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQILMSVRTK 141

Query: 436 DRWKAQVIEALRRAKFKFPGRQKISYQRS-GVSQ 534
           D  +A  IEALRR ++KFPG+Q+I   +  G SQ
Sbjct: 142 DSSRATAIEALRRCQYKFPGQQRIIVSKKWGFSQ 175



 Score = 53.2 bits (122), Expect = 3e-08
 Identities = 21/52 (40%), Positives = 32/52 (61%)
 Frame = +3

Query: 474 CQVQVPRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629
           CQ + P   + +VSKKWGF++Y RDE+ + R  G +  DGC  ++  + G L
Sbjct: 155 CQYKFPGQQRIIVSKKWGFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206


>SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 405

 Score = 29.1 bits (62), Expect = 0.64
 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
 Frame = +2

Query: 38  YRYCKNKPYPKSRFCRGVPDP-KIRIFDLGKKRATVDDFPLCVHLVSDEYEQLSSEALEA 214
           Y+   N     ++FC  V +  KI   +  +KRA +D   LC+  +S + E ++  +LE+
Sbjct: 34  YQKVPNSHKEFTKFCYEVYNEIKISDKEFKEKRAALDTLRLCLKRISPDAELVAFGSLES 93

Query: 215 G 217
           G
Sbjct: 94  G 94


>SPBC1105.03c |mrpl16||mitochondrial ribosomal protein subunit
           L16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 22/92 (23%), Positives = 36/92 (39%), Gaps = 4/92 (4%)
 Frame = +1

Query: 244 KELRKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGT----VARVRIGQ 411
           K+L   +  ++  L P    R+     C        G     GK +G      AR+ IG+
Sbjct: 87  KQLETAEQILKRILKPIKAARVYTRF-CCNVPVCVKGNETRMGKGKGAFEYWAARIPIGR 145

Query: 412 PIMSVRSSDRWKAQVIEALRRAKFKFPGRQKI 507
            +  +      K     AL++A F  PG+ +I
Sbjct: 146 VLFEIGGDGMRKELAEHALKQAAFHLPGKYEI 177


>SPCC1827.04 |||ankyrin repeat protein, unknown biological
           role|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 600

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +2

Query: 95  DPKIRIFDLGKKRATVDDFPLCV-HLVSDEYEQLSSEALEA 214
           DPK+R+F    KRAT  +   C   LV+ +   + S +++A
Sbjct: 320 DPKLRVFPFTTKRATHSELLRCYKELVTPKISHVDSISIKA 360


>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 527

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 29/86 (33%), Positives = 40/86 (46%)
 Frame = +3

Query: 51  KINRIRNRGSVGVYLIPRSVSSIWVRRERPLTTFHCACTWCPTNMNS*AQRLWRQDVSAA 230
           KI R+R+  SV + L   S+S  W  R   L   H   T   +  +  A RL  + VS  
Sbjct: 340 KIKRLRSEPSVSLTL---SIS--WYERFEKL--MHAQNTMLRSAFSHVA-RLPMETVSQL 391

Query: 231 ISTS*RTAKGSVPYPHETSPFPRYPH 308
           +S    T    +P P ETSP P+ P+
Sbjct: 392 LSHYTETISQYLP-PSETSPIPKTPN 416


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 19/54 (35%), Positives = 30/54 (55%), Gaps = 5/54 (9%)
 Frame = +2

Query: 116  DLGKKRATVDDFPLCVHLVSDEYEQL---SSEALEAGRICCN--KYLVKNCERI 262
            ++ KKRA  DD    + +VS EYE L   SS+  ++     N  KY+ KN +++
Sbjct: 1033 EIEKKRAENDDLQSKLSVVSSEYENLLLISSQTNKSLEDKTNQLKYIEKNVQKL 1086


>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 678

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +1

Query: 499 QKISYQRSGVSQSMNVMSLRSCVKRAASLMTA 594
           Q  SYQR  + +  +V+  RSC+ +A +L+ A
Sbjct: 304 QTFSYQRHQIYKEEDVVLARSCIIKARTLIGA 335


>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = +3

Query: 129 RERPLTTFHCACTWCPTNMNS 191
           R RP +T H +CT+C   + S
Sbjct: 459 RSRPDSTSHASCTYCVARLRS 479


>SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1038

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 24/74 (32%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
 Frame = -1

Query: 387  STLRLAKRTTHPSLEPISSS-A**HFIDADNVERVKSHADMELILSQFFTRYLLQQIRPA 211
            +T +LAKR T PSL P  S+    H I  +  ++ KS A+   IL  F  +   +Q    
Sbjct: 957  TTTKLAKRITQPSLSPARSTVTITHNIATEAKKKSKSAAND--ILHLFKKKAKSEQSNAK 1014

Query: 210  SKASELSCSYSSDT 169
            S +S +  S    T
Sbjct: 1015 SSSSSIKESKKMKT 1028


>SPBC1348.02 |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 344

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = -2

Query: 584 SEAALFTQLLKLITFILCETPLL*Y 510
           S+A  F ++L    FI C +P+L Y
Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHY 287


>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1234

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/39 (28%), Positives = 21/39 (53%)
 Frame = -3

Query: 214  CLQSL*AQLFIFVGHQVHAQWKVVNGRSLLTQIEDTDLG 98
            CL  L   L   + H++ ++W+ +N R LL + +  + G
Sbjct: 940  CLLPLLPALLSELDHKLVSEWRRINDRGLLVEEDAEETG 978


>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 3131

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 14/35 (40%), Positives = 20/35 (57%)
 Frame = +1

Query: 256 KDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMR 360
           +D  H+RM ++ FH   IN MLS +  D  +  MR
Sbjct: 859 EDNSHLRMSINSFH---INDMLSKSSRDNNRQLMR 890


>SPBPB2B2.19c |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 344

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = -2

Query: 584 SEAALFTQLLKLITFILCETPLL*Y 510
           S+A  F ++L    FI C +P+L Y
Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHY 287


>SPAC977.01 |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 1||Partial|Manual
          Length = 316

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = -2

Query: 584 SEAALFTQLLKLITFILCETPLL*Y 510
           S+A  F ++L    FI C +P+L Y
Sbjct: 235 SKARFFRRMLNAFIFIFCASPMLHY 259


>SPAC750.05c |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 344

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = -2

Query: 584 SEAALFTQLLKLITFILCETPLL*Y 510
           S+A  F ++L    FI C +P+L Y
Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHY 287


>SPAC3A12.05c |taf2||TATA-binding protein associated factor
           Taf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1174

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 8/15 (53%), Positives = 13/15 (86%)
 Frame = +3

Query: 81  VGVYLIPRSVSSIWV 125
           +GVYLIP++ S +W+
Sbjct: 394 IGVYLIPKAWSDLWL 408


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,145,324
Number of Sequences: 5004
Number of extensions: 67990
Number of successful extensions: 216
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)