BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0139 (699 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g66580.1 68414.m07565 60S ribosomal protein L10 (RPL10C) cont... 136 1e-32 At1g14320.1 68414.m01697 60S ribosomal protein L10 (RPL10A) / Wi... 136 1e-32 At1g26910.1 68414.m03281 60S ribosomal protein L10 (RPL10B) Near... 136 2e-32 At5g47870.1 68418.m05914 expressed protein 30 1.7 At5g24280.1 68418.m02856 expressed protein ; expression supporte... 28 5.2 At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identica... 28 6.8 At4g24120.1 68417.m03462 transporter, putative similar to iron-p... 28 6.8 At3g47340.3 68416.m05147 asparagine synthetase 1 [glutamine-hydr... 28 6.8 At3g47340.2 68416.m05146 asparagine synthetase 1 [glutamine-hydr... 28 6.8 At3g47340.1 68416.m05145 asparagine synthetase 1 [glutamine-hydr... 28 6.8 At1g36070.1 68414.m04484 WD-40 repeat family protein contains 2 ... 28 6.8 At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-pa... 27 9.0 At3g11130.1 68416.m01349 clathrin heavy chain, putative similar ... 27 9.0 At3g08530.1 68416.m00990 clathrin heavy chain, putative similar ... 27 9.0 >At1g66580.1 68414.m07565 60S ribosomal protein L10 (RPL10C) contains Pfam profile: PF00826: Ribosomal L10 Length = 221 Score = 136 bits (329), Expect = 1e-32 Identities = 60/79 (75%), Positives = 67/79 (84%) Frame = +2 Query: 14 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193 MGRRPARCYR K KPYPKSR+CRGVPDPKIRI+D+G KR VD+FP CVHLVS E E + Sbjct: 1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPFCVHLVSWEKENV 60 Query: 194 SSEALEAGRICCNKYLVKN 250 SSEALEA RI CNKY+VK+ Sbjct: 61 SSEALEAARIACNKYMVKS 79 Score = 136 bits (329), Expect = 1e-32 Identities = 65/92 (70%), Positives = 71/92 (77%) Frame = +1 Query: 244 KELRKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMS 423 K KD FH+R+R+HPFHV+RINKMLSCAGADRLQTGMRGAFGK GT ARV IGQ ++S Sbjct: 78 KSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLS 137 Query: 424 VRSSDRWKAQVIEALRRAKFKFPGRQKISYQR 519 VR D EALRRAKFKFPGRQKI R Sbjct: 138 VRCKDNHGVHAQEALRRAKFKFPGRQKIIVSR 169 Score = 51.2 bits (117), Expect = 6e-07 Identities = 22/47 (46%), Positives = 29/47 (61%) Frame = +3 Query: 489 PRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629 P K +VS+KWGFTK+ R E+ KLR R+ DG ++ HGPL Sbjct: 160 PGRQKIIVSRKWGFTKFNRAEYTKLRAMKRIVPDGVNAKFLSNHGPL 206 >At1g14320.1 68414.m01697 60S ribosomal protein L10 (RPL10A) / Wilm's tumor suppressor protein-related similar to tumor suppressor GI:575354 from [Oryza sativa] Length = 220 Score = 136 bits (329), Expect = 1e-32 Identities = 60/79 (75%), Positives = 67/79 (84%) Frame = +2 Query: 14 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193 MGRRPARCYR K KPYPKSR+CRGVPDPKIRI+D+G KR VD+FP CVHLVS E E + Sbjct: 1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPFCVHLVSWEKENV 60 Query: 194 SSEALEAGRICCNKYLVKN 250 SSEALEA RI CNKY+VK+ Sbjct: 61 SSEALEAARIACNKYMVKS 79 Score = 135 bits (326), Expect = 3e-32 Identities = 65/92 (70%), Positives = 71/92 (77%) Frame = +1 Query: 244 KELRKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMS 423 K KD FH+R+R+HPFHV+RINKMLSCAGADRLQTGMRGAFGK GT ARV IGQ ++S Sbjct: 78 KSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLS 137 Query: 424 VRSSDRWKAQVIEALRRAKFKFPGRQKISYQR 519 VR D EALRRAKFKFPGRQKI R Sbjct: 138 VRCKDAHGHHAQEALRRAKFKFPGRQKIIVSR 169 Score = 53.2 bits (122), Expect = 2e-07 Identities = 23/47 (48%), Positives = 31/47 (65%) Frame = +3 Query: 489 PRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629 P K +VS+KWGFTK+ R +F KLR+E R+ DG ++ HGPL Sbjct: 160 PGRQKIIVSRKWGFTKFNRADFTKLRQEKRVVPDGVNAKFLSCHGPL 206 >At1g26910.1 68414.m03281 60S ribosomal protein L10 (RPL10B) Nearly identical to ribosomal protein L10.e, Wilm's tumor suppressor homologue, gi|17682 (Z15157), however differences in sequence indicate this is a different member of the L10 family Length = 221 Score = 136 bits (328), Expect = 2e-32 Identities = 60/79 (75%), Positives = 67/79 (84%) Frame = +2 Query: 14 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193 MGRRPARCYR K KPYPKSR+CRGVPDPKIRI+D+G KR VD+FP CVHLVS E E + Sbjct: 1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPYCVHLVSWEKENV 60 Query: 194 SSEALEAGRICCNKYLVKN 250 SSEALEA RI CNKY+VK+ Sbjct: 61 SSEALEAARIACNKYMVKS 79 Score = 135 bits (326), Expect = 3e-32 Identities = 65/92 (70%), Positives = 71/92 (77%) Frame = +1 Query: 244 KELRKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMS 423 K KD FH+R+R+HPFHV+RINKMLSCAGADRLQTGMRGAFGK GT ARV IGQ ++S Sbjct: 78 KSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLS 137 Query: 424 VRSSDRWKAQVIEALRRAKFKFPGRQKISYQR 519 VR D EALRRAKFKFPGRQKI R Sbjct: 138 VRCKDAHGHHAQEALRRAKFKFPGRQKIIVSR 169 Score = 52.4 bits (120), Expect = 3e-07 Identities = 22/47 (46%), Positives = 31/47 (65%) Frame = +3 Query: 489 PRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629 P K +VS+KWGFTK+ R ++ KLR+E R+ DG ++ HGPL Sbjct: 160 PGRQKIIVSRKWGFTKFNRADYTKLRQEKRIVPDGVNAKFLSCHGPL 206 >At5g47870.1 68418.m05914 expressed protein Length = 199 Score = 29.9 bits (64), Expect = 1.7 Identities = 28/122 (22%), Positives = 43/122 (35%) Frame = +1 Query: 274 RMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMSVRSSDRWKAQ 453 R++L PF + +N+ + C+G G+ G + V PI SS Sbjct: 23 RIKLSPFRTVAVNRGVRCSGG-----GVGGGDAGKKKAVPNSNYVVPIDKFSSSSSITRP 77 Query: 454 VIEALRRAKFKFPGRQKISYQRSGVSQSMNVMSLRSCVKRAASLMTAALCSTARNMDLST 633 +IE LR K P S+ S + + R S C R++ S Sbjct: 78 LIEILRDLNKKIPDNIVKSHDPPSTSAATSGFIPWYHANRMLSFYAPGWCGEVRDVIFSE 137 Query: 634 LG 639 G Sbjct: 138 NG 139 >At5g24280.1 68418.m02856 expressed protein ; expression supported by MPSS Length = 1634 Score = 28.3 bits (60), Expect = 5.2 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +2 Query: 173 SDEYEQLSSEALEAGRICCNKYLV 244 SDEY + SEA GR N++LV Sbjct: 1431 SDEYRKFQSEAASLGRSITNRFLV 1454 >At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identical to asparagine synthetase (ASN3) [Arabidopsis thaliana] GI:3859534 Length = 578 Score = 27.9 bits (59), Expect = 6.8 Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = +1 Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330 +++LGV+ + + E FGG Y + +KE ++ LH + +R NK S Sbjct: 331 IKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAW 390 Query: 331 GAD 339 G + Sbjct: 391 GVE 393 >At4g24120.1 68417.m03462 transporter, putative similar to iron-phytosiderophore transporter protein yellow stripe 1 [Zea mays] GI:10770865; contains Pfam profile PF03169: OPT oligopeptide transporter protein Length = 665 Score = 27.9 bits (59), Expect = 6.8 Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 1/61 (1%) Frame = +2 Query: 239 LVKNCERISSISA*DF-TLSTLSASIKCYHALELIGSRLGCVVRLASLRVL*HVFALDSP 415 L+K+ +S I DF T S K A ++IG+ +GC+V S + F + +P Sbjct: 468 LIKSVVSVSCILMQDFKTAHYTMTSPKAMFASQMIGTVVGCIVTPLSFFLFYKAFDIGNP 527 Query: 416 S 418 + Sbjct: 528 N 528 >At3g47340.3 68416.m05147 asparagine synthetase 1 [glutamine-hydrolyzing] / glutamine-dependent asparagine synthetase 1 (ASN1) identical to SP|P49078 Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) (Glutamine- dependent asparagine synthetase) {Arabidopsis thaliana} Length = 456 Score = 27.9 bits (59), Expect = 6.8 Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = +1 Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330 +++LGV+ + + E FGG Y + +KE ++ LH + +R NK S Sbjct: 331 IKSLGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAF 390 Query: 331 GAD 339 G + Sbjct: 391 GLE 393 >At3g47340.2 68416.m05146 asparagine synthetase 1 [glutamine-hydrolyzing] / glutamine-dependent asparagine synthetase 1 (ASN1) identical to SP|P49078 Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) (Glutamine- dependent asparagine synthetase) {Arabidopsis thaliana} Length = 512 Score = 27.9 bits (59), Expect = 6.8 Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = +1 Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330 +++LGV+ + + E FGG Y + +KE ++ LH + +R NK S Sbjct: 331 IKSLGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAF 390 Query: 331 GAD 339 G + Sbjct: 391 GLE 393 >At3g47340.1 68416.m05145 asparagine synthetase 1 [glutamine-hydrolyzing] / glutamine-dependent asparagine synthetase 1 (ASN1) identical to SP|P49078 Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) (Glutamine- dependent asparagine synthetase) {Arabidopsis thaliana} Length = 584 Score = 27.9 bits (59), Expect = 6.8 Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = +1 Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330 +++LGV+ + + E FGG Y + +KE ++ LH + +R NK S Sbjct: 331 IKSLGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAF 390 Query: 331 GAD 339 G + Sbjct: 391 GLE 393 >At1g36070.1 68414.m04484 WD-40 repeat family protein contains 2 WD-40 repeats (PF0400);similar to guanine nucleotide-binding protein beta subunit GPBA (SP:P36408) [Dictyostelium discoideum (Slime mold)]; similar to katanin p80 (WD40-containing) subunit B 1 (GI:12655011) [Homo sapiens] Length = 418 Score = 27.9 bits (59), Expect = 6.8 Identities = 12/28 (42%), Positives = 15/28 (53%) Frame = +3 Query: 483 QVPRTSKDLVSKKWGFTKYERDEFEKLR 566 Q R KD+ +W KY RDEF + R Sbjct: 26 QEARNGKDIQGIQWEGFKYNRDEFRETR 53 >At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-patch domain-containing protein / RNA recognition motif (RRM)-containing protein KIAA0122 gene , Homo sapiens, EMBL:HSDKG02; contains Pfam profiles PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain), PF01585: G-patch domain, weak hit to PF00641: Zn-finger in Ran binding protein and others Length = 1105 Score = 27.5 bits (58), Expect = 9.0 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%) Frame = -2 Query: 122 PNRRYG-SWDQVHPDRTSISDTVYFCSTGSISLAGAP 15 PN R+G S+D +PD + D VY G +L P Sbjct: 53 PNHRFGVSYDDGYPDERLMRDDVYNYPPGHNTLGDLP 89 >At3g11130.1 68416.m01349 clathrin heavy chain, putative similar to Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo sapiens] Length = 1705 Score = 27.5 bits (58), Expect = 9.0 Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 2/66 (3%) Frame = +1 Query: 370 GKPQGTVARVRIGQPIMSVRSSDRWKAQVIEALRR--AKFKFPGRQKISYQRSGVSQSMN 543 G G+ R ++ + M + S D+ ++Q +EA A+FK PG + S S S+S N Sbjct: 175 GIAPGSPERPQLVKGNMQLFSVDQQRSQALEAHAASFAQFKVPGNENPSILISFASKSFN 234 Query: 544 VMSLRS 561 + S Sbjct: 235 AGQITS 240 >At3g08530.1 68416.m00990 clathrin heavy chain, putative similar to Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo sapiens] Length = 1703 Score = 27.5 bits (58), Expect = 9.0 Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 2/66 (3%) Frame = +1 Query: 370 GKPQGTVARVRIGQPIMSVRSSDRWKAQVIEALRR--AKFKFPGRQKISYQRSGVSQSMN 543 G G+ R ++ + M + S D+ ++Q +EA A+FK PG + S S S+S N Sbjct: 175 GIAPGSPERQQLVKGNMQLFSVDQQRSQALEAHAASFAQFKVPGNENPSILISFASKSFN 234 Query: 544 VMSLRS 561 + S Sbjct: 235 AGQITS 240 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,945,781 Number of Sequences: 28952 Number of extensions: 381469 Number of successful extensions: 1135 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 1092 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1135 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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