BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0139
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g66580.1 68414.m07565 60S ribosomal protein L10 (RPL10C) cont... 136 1e-32
At1g14320.1 68414.m01697 60S ribosomal protein L10 (RPL10A) / Wi... 136 1e-32
At1g26910.1 68414.m03281 60S ribosomal protein L10 (RPL10B) Near... 136 2e-32
At5g47870.1 68418.m05914 expressed protein 30 1.7
At5g24280.1 68418.m02856 expressed protein ; expression supporte... 28 5.2
At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identica... 28 6.8
At4g24120.1 68417.m03462 transporter, putative similar to iron-p... 28 6.8
At3g47340.3 68416.m05147 asparagine synthetase 1 [glutamine-hydr... 28 6.8
At3g47340.2 68416.m05146 asparagine synthetase 1 [glutamine-hydr... 28 6.8
At3g47340.1 68416.m05145 asparagine synthetase 1 [glutamine-hydr... 28 6.8
At1g36070.1 68414.m04484 WD-40 repeat family protein contains 2 ... 28 6.8
At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-pa... 27 9.0
At3g11130.1 68416.m01349 clathrin heavy chain, putative similar ... 27 9.0
At3g08530.1 68416.m00990 clathrin heavy chain, putative similar ... 27 9.0
>At1g66580.1 68414.m07565 60S ribosomal protein L10 (RPL10C)
contains Pfam profile: PF00826: Ribosomal L10
Length = 221
Score = 136 bits (329), Expect = 1e-32
Identities = 60/79 (75%), Positives = 67/79 (84%)
Frame = +2
Query: 14 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193
MGRRPARCYR K KPYPKSR+CRGVPDPKIRI+D+G KR VD+FP CVHLVS E E +
Sbjct: 1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPFCVHLVSWEKENV 60
Query: 194 SSEALEAGRICCNKYLVKN 250
SSEALEA RI CNKY+VK+
Sbjct: 61 SSEALEAARIACNKYMVKS 79
Score = 136 bits (329), Expect = 1e-32
Identities = 65/92 (70%), Positives = 71/92 (77%)
Frame = +1
Query: 244 KELRKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMS 423
K KD FH+R+R+HPFHV+RINKMLSCAGADRLQTGMRGAFGK GT ARV IGQ ++S
Sbjct: 78 KSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLS 137
Query: 424 VRSSDRWKAQVIEALRRAKFKFPGRQKISYQR 519
VR D EALRRAKFKFPGRQKI R
Sbjct: 138 VRCKDNHGVHAQEALRRAKFKFPGRQKIIVSR 169
Score = 51.2 bits (117), Expect = 6e-07
Identities = 22/47 (46%), Positives = 29/47 (61%)
Frame = +3
Query: 489 PRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629
P K +VS+KWGFTK+ R E+ KLR R+ DG ++ HGPL
Sbjct: 160 PGRQKIIVSRKWGFTKFNRAEYTKLRAMKRIVPDGVNAKFLSNHGPL 206
>At1g14320.1 68414.m01697 60S ribosomal protein L10 (RPL10A) /
Wilm's tumor suppressor protein-related similar to tumor
suppressor GI:575354 from [Oryza sativa]
Length = 220
Score = 136 bits (329), Expect = 1e-32
Identities = 60/79 (75%), Positives = 67/79 (84%)
Frame = +2
Query: 14 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193
MGRRPARCYR K KPYPKSR+CRGVPDPKIRI+D+G KR VD+FP CVHLVS E E +
Sbjct: 1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPFCVHLVSWEKENV 60
Query: 194 SSEALEAGRICCNKYLVKN 250
SSEALEA RI CNKY+VK+
Sbjct: 61 SSEALEAARIACNKYMVKS 79
Score = 135 bits (326), Expect = 3e-32
Identities = 65/92 (70%), Positives = 71/92 (77%)
Frame = +1
Query: 244 KELRKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMS 423
K KD FH+R+R+HPFHV+RINKMLSCAGADRLQTGMRGAFGK GT ARV IGQ ++S
Sbjct: 78 KSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLS 137
Query: 424 VRSSDRWKAQVIEALRRAKFKFPGRQKISYQR 519
VR D EALRRAKFKFPGRQKI R
Sbjct: 138 VRCKDAHGHHAQEALRRAKFKFPGRQKIIVSR 169
Score = 53.2 bits (122), Expect = 2e-07
Identities = 23/47 (48%), Positives = 31/47 (65%)
Frame = +3
Query: 489 PRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629
P K +VS+KWGFTK+ R +F KLR+E R+ DG ++ HGPL
Sbjct: 160 PGRQKIIVSRKWGFTKFNRADFTKLRQEKRVVPDGVNAKFLSCHGPL 206
>At1g26910.1 68414.m03281 60S ribosomal protein L10 (RPL10B) Nearly
identical to ribosomal protein L10.e, Wilm's tumor
suppressor homologue, gi|17682 (Z15157), however
differences in sequence indicate this is a different
member of the L10 family
Length = 221
Score = 136 bits (328), Expect = 2e-32
Identities = 60/79 (75%), Positives = 67/79 (84%)
Frame = +2
Query: 14 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 193
MGRRPARCYR K KPYPKSR+CRGVPDPKIRI+D+G KR VD+FP CVHLVS E E +
Sbjct: 1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPYCVHLVSWEKENV 60
Query: 194 SSEALEAGRICCNKYLVKN 250
SSEALEA RI CNKY+VK+
Sbjct: 61 SSEALEAARIACNKYMVKS 79
Score = 135 bits (326), Expect = 3e-32
Identities = 65/92 (70%), Positives = 71/92 (77%)
Frame = +1
Query: 244 KELRKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMS 423
K KD FH+R+R+HPFHV+RINKMLSCAGADRLQTGMRGAFGK GT ARV IGQ ++S
Sbjct: 78 KSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLS 137
Query: 424 VRSSDRWKAQVIEALRRAKFKFPGRQKISYQR 519
VR D EALRRAKFKFPGRQKI R
Sbjct: 138 VRCKDAHGHHAQEALRRAKFKFPGRQKIIVSR 169
Score = 52.4 bits (120), Expect = 3e-07
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +3
Query: 489 PRTSKDLVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 629
P K +VS+KWGFTK+ R ++ KLR+E R+ DG ++ HGPL
Sbjct: 160 PGRQKIIVSRKWGFTKFNRADYTKLRQEKRIVPDGVNAKFLSCHGPL 206
>At5g47870.1 68418.m05914 expressed protein
Length = 199
Score = 29.9 bits (64), Expect = 1.7
Identities = 28/122 (22%), Positives = 43/122 (35%)
Frame = +1
Query: 274 RMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMSVRSSDRWKAQ 453
R++L PF + +N+ + C+G G+ G + V PI SS
Sbjct: 23 RIKLSPFRTVAVNRGVRCSGG-----GVGGGDAGKKKAVPNSNYVVPIDKFSSSSSITRP 77
Query: 454 VIEALRRAKFKFPGRQKISYQRSGVSQSMNVMSLRSCVKRAASLMTAALCSTARNMDLST 633
+IE LR K P S+ S + + R S C R++ S
Sbjct: 78 LIEILRDLNKKIPDNIVKSHDPPSTSAATSGFIPWYHANRMLSFYAPGWCGEVRDVIFSE 137
Query: 634 LG 639
G
Sbjct: 138 NG 139
>At5g24280.1 68418.m02856 expressed protein ; expression supported by
MPSS
Length = 1634
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +2
Query: 173 SDEYEQLSSEALEAGRICCNKYLV 244
SDEY + SEA GR N++LV
Sbjct: 1431 SDEYRKFQSEAASLGRSITNRFLV 1454
>At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identical
to asparagine synthetase (ASN3) [Arabidopsis thaliana]
GI:3859534
Length = 578
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330
+++LGV+ + + E FGG Y + +KE ++ LH + +R NK S
Sbjct: 331 IKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAW 390
Query: 331 GAD 339
G +
Sbjct: 391 GVE 393
>At4g24120.1 68417.m03462 transporter, putative similar to
iron-phytosiderophore transporter protein yellow stripe
1 [Zea mays] GI:10770865; contains Pfam profile PF03169:
OPT oligopeptide transporter protein
Length = 665
Score = 27.9 bits (59), Expect = 6.8
Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = +2
Query: 239 LVKNCERISSISA*DF-TLSTLSASIKCYHALELIGSRLGCVVRLASLRVL*HVFALDSP 415
L+K+ +S I DF T S K A ++IG+ +GC+V S + F + +P
Sbjct: 468 LIKSVVSVSCILMQDFKTAHYTMTSPKAMFASQMIGTVVGCIVTPLSFFLFYKAFDIGNP 527
Query: 416 S 418
+
Sbjct: 528 N 528
>At3g47340.3 68416.m05147 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 456
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330
+++LGV+ + + E FGG Y + +KE ++ LH + +R NK S
Sbjct: 331 IKSLGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAF 390
Query: 331 GAD 339
G +
Sbjct: 391 GLE 393
>At3g47340.2 68416.m05146 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 512
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330
+++LGV+ + + E FGG Y + +KE ++ LH + +R NK S
Sbjct: 331 IKSLGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAF 390
Query: 331 GAD 339
G +
Sbjct: 391 GLE 393
>At3g47340.1 68416.m05145 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 584
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 VRALGVRRI*TAELRG--FGGRTYLLQ*VPRKELRKDQFHIRMRLHPFHVIRINKMLSCA 330
+++LGV+ + + E FGG Y + +KE ++ LH + +R NK S
Sbjct: 331 IKSLGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAF 390
Query: 331 GAD 339
G +
Sbjct: 391 GLE 393
>At1g36070.1 68414.m04484 WD-40 repeat family protein contains 2
WD-40 repeats (PF0400);similar to guanine
nucleotide-binding protein beta subunit GPBA (SP:P36408)
[Dictyostelium discoideum (Slime mold)]; similar to
katanin p80 (WD40-containing) subunit B 1 (GI:12655011)
[Homo sapiens]
Length = 418
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 483 QVPRTSKDLVSKKWGFTKYERDEFEKLR 566
Q R KD+ +W KY RDEF + R
Sbjct: 26 QEARNGKDIQGIQWEGFKYNRDEFRETR 53
>At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-patch
domain-containing protein / RNA recognition motif
(RRM)-containing protein KIAA0122 gene , Homo sapiens,
EMBL:HSDKG02; contains Pfam profiles PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF01585: G-patch domain, weak hit to PF00641: Zn-finger
in Ran binding protein and others
Length = 1105
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -2
Query: 122 PNRRYG-SWDQVHPDRTSISDTVYFCSTGSISLAGAP 15
PN R+G S+D +PD + D VY G +L P
Sbjct: 53 PNHRFGVSYDDGYPDERLMRDDVYNYPPGHNTLGDLP 89
>At3g11130.1 68416.m01349 clathrin heavy chain, putative similar to
Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo
sapiens]
Length = 1705
Score = 27.5 bits (58), Expect = 9.0
Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +1
Query: 370 GKPQGTVARVRIGQPIMSVRSSDRWKAQVIEALRR--AKFKFPGRQKISYQRSGVSQSMN 543
G G+ R ++ + M + S D+ ++Q +EA A+FK PG + S S S+S N
Sbjct: 175 GIAPGSPERPQLVKGNMQLFSVDQQRSQALEAHAASFAQFKVPGNENPSILISFASKSFN 234
Query: 544 VMSLRS 561
+ S
Sbjct: 235 AGQITS 240
>At3g08530.1 68416.m00990 clathrin heavy chain, putative similar to
Swiss-Prot:Q00610 clathrin heavy chain 1 (CLH-17) [Homo
sapiens]
Length = 1703
Score = 27.5 bits (58), Expect = 9.0
Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +1
Query: 370 GKPQGTVARVRIGQPIMSVRSSDRWKAQVIEALRR--AKFKFPGRQKISYQRSGVSQSMN 543
G G+ R ++ + M + S D+ ++Q +EA A+FK PG + S S S+S N
Sbjct: 175 GIAPGSPERQQLVKGNMQLFSVDQQRSQALEAHAASFAQFKVPGNENPSILISFASKSFN 234
Query: 544 VMSLRS 561
+ S
Sbjct: 235 AGQITS 240
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,945,781
Number of Sequences: 28952
Number of extensions: 381469
Number of successful extensions: 1135
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1092
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1135
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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