Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0135
(748 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC16E8.11c |tfb1||transcription factor TFIIH complex subunit T... 30 0.30
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 28 1.6
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 27 2.8
SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces pomb... 27 3.8
SPAC750.03c |||methyltransferase |Schizosaccharomyces pombe|chr ... 26 6.6
SPBC1348.04 |||methyltransferase |Schizosaccharomyces pombe|chr ... 26 6.6
SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 26 6.6
SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 25 8.7
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 8.7
SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr 3... 25 8.7
>SPAC16E8.11c |tfb1||transcription factor TFIIH complex subunit
Tfb1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 477
Score = 30.3 bits (65), Expect = 0.30
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +2
Query: 437 RVFLLICSDGELPFKVAVHLTATSQARTHCD 529
RVF+++ +GE P + H T T AR +CD
Sbjct: 3 RVFIVV-KEGEDPTSLVFHFTGTPNARENCD 32
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 27.9 bits (59), Expect = 1.6
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +2
Query: 263 PVVVSQVKYLMFTLPLTIPNSLRRITTRM*ASYE*HMPSTSAQISSRV 406
P ++ V YL+ T+ T+P +L I M A+ + PS A + R+
Sbjct: 288 PNILQSVHYLISTISATLPRTLYNIVLFMVAAAKTVAPSVFATFAFRI 335
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 27.1 bits (57), Expect = 2.8
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +2
Query: 206 TCFHGEFYDPAYRRIIVDLPVVVSQVKYLMFTLP 307
+C H + DP+Y I VD +V S V Y +F P
Sbjct: 210 SCRHAQSLDPSYASINVDEQIVDSMV-YRLFREP 242
>SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1066
Score = 26.6 bits (56), Expect = 3.8
Identities = 15/36 (41%), Positives = 17/36 (47%)
Frame = -3
Query: 500 PSDALPP*TVVPHPSRSTKIPWGITTPCWIIAPCWI 393
PSDA T VP + S PW TTP P W+
Sbjct: 21 PSDAQSSPTFVPSANPSLTTPWLQTTPS-ANRPTWL 55
>SPAC750.03c |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 145
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -2
Query: 570 DHDPYTGSCSFLCWS 526
DH+PYT S S +C S
Sbjct: 91 DHEPYTASLSHICHS 105
>SPBC1348.04 |||methyltransferase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 145
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -2
Query: 570 DHDPYTGSCSFLCWS 526
DH+PYT S S +C S
Sbjct: 91 DHEPYTASLSHICHS 105
>SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2386
Score = 25.8 bits (54), Expect = 6.6
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +3
Query: 210 VSMENSTILHTVALSWI 260
+++EN+ IL TV LSWI
Sbjct: 629 LAVENTAILETVILSWI 645
>SPBC13G1.14c |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 243
Score = 25.4 bits (53), Expect = 8.7
Identities = 13/43 (30%), Positives = 19/43 (44%)
Frame = -2
Query: 693 PKDTMPTNAFWKKAGHPSQYYRSHGHHPGPNRRRICDQSRRDH 565
P + + + K+ H S+Y R+ PG N DQS H
Sbjct: 195 PSRSQNNSHYNDKSFHRSRYSRARSRSPGSNISEYSDQSPPYH 237
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 25.4 bits (53), Expect = 8.7
Identities = 16/66 (24%), Positives = 25/66 (37%), Gaps = 3/66 (4%)
Frame = +1
Query: 154 PTVRWYCSHQLPL---PFNCYLFPWRILRSCIPSHYRGSSRRSEPGEISYVHFAVNHPQF 324
PT W+ + P N Y R SCI ++Y + G S+ F++ Q
Sbjct: 35 PTFFWFAHDETPSFKWVLNAYHERLRSCTSCIQAYYELRNESLAKGSYSFTGFSITDLQE 94
Query: 325 SEENYD 342
+D
Sbjct: 95 KWNKWD 100
>SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 437
Score = 25.4 bits (53), Expect = 8.7
Identities = 17/51 (33%), Positives = 22/51 (43%), Gaps = 6/51 (11%)
Frame = -2
Query: 693 PKDTMPTNA--FWKKAGHPSQYY----RSHGHHPGPNRRRICDQSRRDHDP 559
P + T+A ++ S YY R HG P P RR + RR DP
Sbjct: 137 PYSSYSTDAPHYYHAGSESSAYYDSRSRLHGIQPPPKRRTLSPPPRRLADP 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,353,124
Number of Sequences: 5004
Number of extensions: 76260
Number of successful extensions: 216
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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