Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0134
(634 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q16822 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 180 3e-44
UniRef50_Q9HLV2 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 116 4e-25
UniRef50_UPI000038E5C0 Cluster: hypothetical protein Faci_030001... 110 2e-23
UniRef50_Q8FM16 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 99 4e-20
UniRef50_A1SQ84 Cluster: Phosphoenolpyruvate carboxykinase; n=2;... 94 2e-18
UniRef50_A7HGY6 Cluster: Phosphoenolpyruvate carboxykinase; n=16... 93 4e-18
UniRef50_Q4J9S8 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 52 9e-06
UniRef50_Q8U410 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 50 5e-05
UniRef50_P91756 Cluster: Phosphoenolpyruvate carboxykinase; n=1;... 43 0.007
UniRef50_A1S0E8 Cluster: Phosphoenolpyruvate carboxykinase; n=2;... 39 0.087
UniRef50_Q9TYQ8 Cluster: Putative uncharacterized protein; n=2; ... 37 0.35
UniRef50_Q5MGN2 Cluster: Putative uncharacterized protein; n=1; ... 37 0.46
UniRef50_UPI0000E4A8D7 Cluster: PREDICTED: similar to Pck1 prote... 36 1.1
UniRef50_A1IAX6 Cluster: Phosphoenolpyruvate carboxykinase; n=2;... 36 1.1
UniRef50_Q5FQT5 Cluster: Putative uncharacterized protein; n=1; ... 33 4.3
UniRef50_A0WCU6 Cluster: Phosphoenolpyruvate carboxykinase; n=1;... 33 7.5
>UniRef50_Q16822 Cluster: Phosphoenolpyruvate carboxykinase [GTP],
mitochondrial precursor; n=571; cellular organisms|Rep:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
precursor - Homo sapiens (Human)
Length = 640
Score = 180 bits (437), Expect = 3e-44
Identities = 79/130 (60%), Positives = 97/130 (74%), Gaps = 1/130 (0%)
Frame = +1
Query: 64 MXSDAKAAAEHSGKMVMHDPFAMRPFFGYNFGDYLKHWLSMP-QPGRNMPKIFHVNWFRK 240
M S++ AAAEH GK++MHDPFAMRPFFGYNFG YL+HWLSM + G +P+IFHVNWFR+
Sbjct: 478 MRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRR 537
Query: 241 DEQGNSSGPVSDENSRVLDWILRRCXXEPCHAETPLGYIPRAGALNTENLSAVDMNELFS 420
DE G+ P EN+RVLDWI RR E ETP+G +P+ GAL+ L A+D +LFS
Sbjct: 538 DEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFS 597
Query: 421 IPKDFWLQEV 450
+PKDFW QEV
Sbjct: 598 LPKDFWEQEV 607
>UniRef50_Q9HLV2 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=3; Thermoplasma|Rep: Phosphoenolpyruvate carboxykinase
[GTP] - Thermoplasma acidophilum
Length = 588
Score = 116 bits (280), Expect = 4e-25
Identities = 56/129 (43%), Positives = 74/129 (57%)
Frame = +1
Query: 64 MXSDAKAAAEHSGKMVMHDPFAMRPFFGYNFGDYLKHWLSMPQPGRNMPKIFHVNWFRKD 243
M + AA+E + DP AMRPF GYN DY +HW+ M + PKIF+VNWFR+
Sbjct: 431 MGVEKTAASEGKVGELRRDPMAMRPFCGYNISDYFRHWIEMGRKLSRRPKIFYVNWFRRR 490
Query: 244 EQGNSSGPVSDENSRVLDWILRRCXXEPCHAETPLGYIPRAGALNTENLSAVDMNELFSI 423
+ G+ P EN RV++WIL R ETP+GYIP + NL+ DM ELF I
Sbjct: 491 QDGSFIWPGFSENFRVIEWILYRLDHNDNAIETPIGYIPENINTDGLNLTKQDMEELFRI 550
Query: 424 PKDFWLQEV 450
+D W +E+
Sbjct: 551 DRDGWREEM 559
>UniRef50_UPI000038E5C0 Cluster: hypothetical protein Faci_03000127;
n=1; Ferroplasma acidarmanus fer1|Rep: hypothetical
protein Faci_03000127 - Ferroplasma acidarmanus fer1
Length = 598
Score = 110 bits (265), Expect = 2e-23
Identities = 54/128 (42%), Positives = 75/128 (58%), Gaps = 2/128 (1%)
Frame = +1
Query: 76 AKAAAEHSGKM--VMHDPFAMRPFFGYNFGDYLKHWLSMPQPGRNMPKIFHVNWFRKDEQ 249
A+ A +GK+ + +DP AM PF GYN GDY +HWL M + ++ P+IF+VNWFRKD
Sbjct: 439 AETTAATTGKVGILRNDPMAMIPFCGYNIGDYFQHWLDMGKLVQHRPEIFYVNWFRKDSD 498
Query: 250 GNSSGPVSDENSRVLDWILRRCXXEPCHAETPLGYIPRAGALNTENLSAVDMNELFSIPK 429
GN P EN RV++WI R + ETP+G IP + NL + +LF I
Sbjct: 499 GNFIWPGFSENFRVIEWIASRLDAKANAIETPVGLIPDIANFASGNLGKNKLEQLFEIDY 558
Query: 430 DFWLQEVS 453
WL+E++
Sbjct: 559 PGWLKELN 566
>UniRef50_Q8FM16 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=116; Bacteria|Rep: Phosphoenolpyruvate carboxykinase
[GTP] - Corynebacterium efficiens
Length = 612
Score = 99 bits (238), Expect = 4e-20
Identities = 52/126 (41%), Positives = 69/126 (54%), Gaps = 3/126 (2%)
Frame = +1
Query: 82 AAAEHSGKMVMHDPFAMRPFFGYNFGDYLKHWLSMPQPGRN-MPKIFHVNWFRKDEQGNS 258
A+AE + HDP AM PF GYN G+YL++W+ M G + MP IF VNWFR+ E G
Sbjct: 452 ASAEAKVGTLRHDPMAMLPFMGYNAGEYLQNWIDMGNKGGDKMPSIFLVNWFRRGEDGRF 511
Query: 259 SGPVSDENSRVLDWILRRCXXEPCHAETPLGYIPRAGALNTENLSA--VDMNELFSIPKD 432
P ENSRVL W++ R ET +GY RA L+ E L D+ E + P +
Sbjct: 512 LWPGFGENSRVLKWVIDRIEGRVGAEETVVGYTARAEDLDLEGLDTPIEDIREALTAPAE 571
Query: 433 FWLQEV 450
W ++
Sbjct: 572 QWAADL 577
>UniRef50_A1SQ84 Cluster: Phosphoenolpyruvate carboxykinase; n=2;
Nocardioides sp. JS614|Rep: Phosphoenolpyruvate
carboxykinase - Nocardioides sp. (strain BAA-499 /
JS614)
Length = 617
Score = 94.3 bits (224), Expect = 2e-18
Identities = 46/135 (34%), Positives = 73/135 (54%), Gaps = 3/135 (2%)
Frame = +1
Query: 64 MXSDAKAAAEHSGKMVMHDPFAMRPFFGYNFGDYLKHWLSMPQPGRNMPKIFHVNWFRKD 243
+ ++A AAA+ ++ +DP +MRPF Y DY +HWL++ RN P HVNWF++
Sbjct: 453 LGAEATAAADGLEGVLRYDPMSMRPFMSYPEADYAQHWLNVIAGARNKPIFAHVNWFQRG 512
Query: 244 EQGNSSGPVSDENSRVLDWILRRCXXEPCHAETPLGYIPRAGALNTENL---SAVDMNEL 414
+ G P EN R L W+++ E ETP+G +P LN + L + D++ +
Sbjct: 513 DDGRFLWPGYRENLRPLVWLMQLKNGEVTGVETPVGILPSRDELNLDGLDEQTLADLDAV 572
Query: 415 FSIPKDFWLQEVSYR 459
+I W QE+ +R
Sbjct: 573 LTIDVPRWKQEIGHR 587
>UniRef50_A7HGY6 Cluster: Phosphoenolpyruvate carboxykinase; n=16;
cellular organisms|Rep: Phosphoenolpyruvate
carboxykinase - Anaeromyxobacter sp. Fw109-5
Length = 595
Score = 93.5 bits (222), Expect = 4e-18
Identities = 47/111 (42%), Positives = 60/111 (54%)
Frame = +1
Query: 64 MXSDAKAAAEHSGKMVMHDPFAMRPFFGYNFGDYLKHWLSMPQPGRNMPKIFHVNWFRKD 243
M S+ AAA + +V DP AM PF GY+ G Y +HWL M N PK++ VNWFRK
Sbjct: 429 MGSETTAAATGAVGIVRRDPMAMLPFCGYDAGTYFQHWLDMQSRIPNPPKVYMVNWFRKS 488
Query: 244 EQGNSSGPVSDENSRVLDWILRRCXXEPCHAETPLGYIPRAGALNTENLSA 396
+ G P +N RVL W+L R ET LG P+AG L+ + A
Sbjct: 489 DDGKFLWPGYGDNMRVLKWMLDRAAGRVGAQETLLGNTPKAGDLDLSGIDA 539
>UniRef50_Q4J9S8 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=4; Sulfolobaceae|Rep: Phosphoenolpyruvate
carboxykinase [GTP] - Sulfolobus acidocaldarius
Length = 604
Score = 52.4 bits (120), Expect = 9e-06
Identities = 32/100 (32%), Positives = 52/100 (52%), Gaps = 1/100 (1%)
Frame = +1
Query: 64 MXSDAKAAAEHSGKMVMHDPFAMRPFFGYNFGDYLKHWLSMPQPGRNMPKIFHVNWFRKD 243
M S +A ++ +P A+ F + G YL ++L+ + + +PKIF N+F KD
Sbjct: 423 MESSRTSAVIGKADVLEFNPMAILDFMPLSLGKYLNNYLTFGRNLKYVPKIFSFNYFLKD 482
Query: 244 EQGNSSGPVSDENSRV-LDWILRRCXXEPCHAETPLGYIP 360
E N+ S E+ RV + W ++R E TP+G+IP
Sbjct: 483 E--NNKFLNSKEDKRVWVKWAVKRVESETDAIYTPVGFIP 520
>UniRef50_Q8U410 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=6; cellular organisms|Rep: Phosphoenolpyruvate
carboxykinase [GTP] - Pyrococcus furiosus
Length = 624
Score = 50.0 bits (114), Expect = 5e-05
Identities = 31/100 (31%), Positives = 50/100 (50%)
Frame = +1
Query: 64 MXSDAKAAAEHSGKMVMHDPFAMRPFFGYNFGDYLKHWLSMPQPGRNMPKIFHVNWFRKD 243
+ S+ AA + +P ++ F + GDYL+++L + + PKIF VN+F +
Sbjct: 445 LESETTAATLGKEGVRAFNPMSILDFLSVHIGDYLRNYLEFGRKLKKTPKIFAVNYFLR- 503
Query: 244 EQGNSSGPVSDENSRVLDWILRRCXXEPCHAETPLGYIPR 363
E G D+ + L W+ R + ETP+GYIPR
Sbjct: 504 ENGQWLNEKLDK-AVWLKWMELRVHNDVGAIETPIGYIPR 542
>UniRef50_P91756 Cluster: Phosphoenolpyruvate carboxykinase; n=1;
Lumbricus rubellus|Rep: Phosphoenolpyruvate
carboxykinase - Lumbricus rubellus (Humus earthworm)
Length = 71
Score = 42.7 bits (96), Expect = 0.007
Identities = 12/38 (31%), Positives = 28/38 (73%)
Frame = +1
Query: 340 TPLGYIPRAGALNTENLSAVDMNELFSIPKDFWLQEVS 453
+P+G +P+ G ++ + L VD N++F++PK +W ++++
Sbjct: 2 SPIGLVPQEGTISGDGLGKVDWNQMFALPKAYWTEDIA 39
>UniRef50_A1S0E8 Cluster: Phosphoenolpyruvate carboxykinase; n=2;
Thermoprotei|Rep: Phosphoenolpyruvate carboxykinase -
Thermofilum pendens (strain Hrk 5)
Length = 636
Score = 39.1 bits (87), Expect = 0.087
Identities = 24/81 (29%), Positives = 39/81 (48%)
Frame = +1
Query: 118 DPFAMRPFFGYNFGDYLKHWLSMPQPGRNMPKIFHVNWFRKDEQGNSSGPVSDENSRVLD 297
+P+A+ F + G + + R PKIF VN+F + + GN D+ + L
Sbjct: 473 NPYAILDFLPISPGAFTELHFRFAGKLRVTPKIFGVNYFLRGKDGNYLAEKRDKLA-WLR 531
Query: 298 WILRRCXXEPCHAETPLGYIP 360
W+ R E +TP+GY+P
Sbjct: 532 WMEARVNGEVGAVKTPVGYVP 552
>UniRef50_Q9TYQ8 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 624
Score = 37.1 bits (82), Expect = 0.35
Identities = 29/129 (22%), Positives = 56/129 (43%), Gaps = 12/129 (9%)
Frame = +1
Query: 103 KMVMHDPFAMRPFFGYNFGDYLKHWLSM----PQPGRNM-----PKIFHVNWFRKDEQGN 255
++++ P ++ Y+KHWL M N P+IF N + ++ G
Sbjct: 472 RILVECPMLRADAINFSIAKYVKHWLEMGVGVKSSSENFENPPPPQIFFTNLY-QEVDGK 530
Query: 256 SSGPVSDENSRVLDWILRRCXXEPCHAET---PLGYIPRAGALNTENLSAVDMNELFSIP 426
P +N+R+ ++I RC ++T LG +P+ L+ + ++ L +
Sbjct: 531 PLWPGGVDNARIFEYIYERCANPADLSKTISSGLGIVPKTLQLS----AGTNLAPLLQVD 586
Query: 427 KDFWLQEVS 453
FWL E++
Sbjct: 587 IRFWLTELN 595
>UniRef50_Q5MGN2 Cluster: Putative uncharacterized protein; n=1;
Lonomia obliqua|Rep: Putative uncharacterized protein -
Lonomia obliqua (Moth)
Length = 53
Score = 36.7 bits (81), Expect = 0.46
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = +1
Query: 397 VDMNELFSIPKDFWLQE 447
+DM LFSIPKDFWLQE
Sbjct: 3 IDMKSLFSIPKDFWLQE 19
>UniRef50_UPI0000E4A8D7 Cluster: PREDICTED: similar to Pck1 protein;
n=3; Strongylocentrotus purpuratus|Rep: PREDICTED:
similar to Pck1 protein - Strongylocentrotus purpuratus
Length = 667
Score = 35.5 bits (78), Expect = 1.1
Identities = 13/16 (81%), Positives = 16/16 (100%)
Frame = +1
Query: 103 KMVMHDPFAMRPFFGY 150
K++M+DPFAMRPFFGY
Sbjct: 650 KVMMNDPFAMRPFFGY 665
>UniRef50_A1IAX6 Cluster: Phosphoenolpyruvate carboxykinase; n=2;
Desulfobacterales|Rep: Phosphoenolpyruvate carboxykinase
- Candidatus Desulfococcus oleovorans Hxd3
Length = 649
Score = 35.5 bits (78), Expect = 1.1
Identities = 28/104 (26%), Positives = 46/104 (44%), Gaps = 8/104 (7%)
Frame = +1
Query: 76 AKAAAEHSGKMVMHDPFAMRPFFGYNFGDYLK---HWLSMPQPGRNM-PKIFHVNWFRKD 243
A A E V P+A PF GDY+ + + P+ + P + +N+F D
Sbjct: 463 AATATEVGASGVKRAPWANAPFIPGALGDYMAAQFEFFASPKIAADKRPVMAGLNYFLTD 522
Query: 244 E-QGNSSGPVSDENSRV---LDWILRRCXXEPCHAETPLGYIPR 363
E +G ++ + E V L W+ R + ETP+G +P+
Sbjct: 523 EARGGTTKKLLGEKKDVKVWLSWLERMAHNDVSFIETPIGNLPK 566
>UniRef50_Q5FQT5 Cluster: Putative uncharacterized protein; n=1;
Gluconobacter oxydans|Rep: Putative uncharacterized
protein - Gluconobacter oxydans (Gluconobacter
suboxydans)
Length = 341
Score = 33.5 bits (73), Expect = 4.3
Identities = 21/62 (33%), Positives = 31/62 (50%)
Frame = -1
Query: 451 SPPAAKSL*VC*TARSCRQRLGFPYSEHRLSVCTPVESRRGRAPRXSDARSSLRPESSRP 272
+PP ++L R+ Q P E RLS+ T +E++R A R S A+ +L P
Sbjct: 61 APPPGENLRYPHVGRTPTQAPDLPTPEARLSLTTQLEAQRNLAQRESAAQGALPTIPPPP 120
Query: 271 KP 266
KP
Sbjct: 121 KP 122
>UniRef50_A0WCU6 Cluster: Phosphoenolpyruvate carboxykinase; n=1;
Geobacter lovleyi SZ|Rep: Phosphoenolpyruvate
carboxykinase - Geobacter lovleyi SZ
Length = 97
Score = 32.7 bits (71), Expect = 7.5
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = +1
Query: 337 ETPLGYIPRAGALNTENL--SAVDMNELFSIPKDFWLQEV 450
E PLG++PR ++ E + S NEL S+ +D W EV
Sbjct: 9 ECPLGWMPRYEDMDWEGIEMSREKFNELMSVDRDVWQNEV 48
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 615,573,241
Number of Sequences: 1657284
Number of extensions: 12434008
Number of successful extensions: 35408
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 34202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35388
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 46881492319
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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