BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0134 (634 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 28 1.3 SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 27 1.7 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 26 3.9 SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|ch... 26 5.2 SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase ... 25 6.9 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 6.9 SPBC2F12.10 |||mitochondrial ribosomal protein subunit L35|Schiz... 25 9.1 SPBC18E5.10 |||iron sulfur cluster assembly protein |Schizosacch... 25 9.1 SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|... 25 9.1 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 27.9 bits (59), Expect = 1.3 Identities = 23/63 (36%), Positives = 29/63 (46%), Gaps = 2/63 (3%) Frame = -3 Query: 413 SSFMSTALRFSVFRA--PALGMYPSGVSAWQGSXXQRRKIQSKTREFSSETGPEELPCSS 240 +S M +VFRA P L + G S W S ++ K TRE E GPE + S Sbjct: 659 TSIMPVGSSINVFRASNPLLDAW-KGASEW--SVTEKFKAAKVTREEYLEKGPEYIKEHS 715 Query: 239 LRN 231 L N Sbjct: 716 LGN 718 >SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 499 Score = 27.5 bits (58), Expect = 1.7 Identities = 20/76 (26%), Positives = 34/76 (44%) Frame = -3 Query: 422 MLNSSFMSTALRFSVFRAPALGMYPSGVSAWQGSXXQRRKIQSKTREFSSETGPEELPCS 243 M+ S +A +F + A G+ P+G G K++ + R+F E G EE+P + Sbjct: 195 MITSRSWESA-KFKSYNFAAEGIPPAG-----GCLHPLMKVREEFRKFFFELGFEEMPTN 248 Query: 242 SLRNQLTWKILGMFRP 195 + W +F P Sbjct: 249 NFVESGFWNFDALFVP 264 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 26.2 bits (55), Expect = 3.9 Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%) Frame = +3 Query: 330 PRRDSTGVHTESRCSEYGKPKRCRHE-RAVQH 422 P+R STG+H S + + P R H+ A+ H Sbjct: 264 PKRQSTGLHRRSLTNYFKFPHRNAHQHNAIAH 295 >SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/35 (34%), Positives = 20/35 (57%), Gaps = 3/35 (8%) Frame = +2 Query: 257 PLARFRTRTLGS*TGSCVAATXSPAT---PRLHWG 352 PL F +TLG+ G + A+ +PA +++WG Sbjct: 129 PLVPFAVKTLGTAAGVMITASHNPAAYNGYKVYWG 163 >SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase Atp23|Schizosaccharomyces pombe|chr 3|||Manual Length = 185 Score = 25.4 bits (53), Expect = 6.9 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = +1 Query: 322 EPCHAETPLGYIPRAGALNTEN 387 +PC A++ GYIP G + EN Sbjct: 51 QPCDAQSTGGYIPGKGIVLCEN 72 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 6.9 Identities = 10/42 (23%), Positives = 20/42 (47%) Frame = +1 Query: 223 VNWFRKDEQGNSSGPVSDENSRVLDWILRRCXXEPCHAETPL 348 +++ + + S + SR+L+ LR+C PC P+ Sbjct: 85 LSYAKHPRKAKSDAEFLNFRSRMLELFLRQCLLHPCIRSNPI 126 >SPBC2F12.10 |||mitochondrial ribosomal protein subunit L35|Schizosaccharomyces pombe|chr 2|||Manual Length = 370 Score = 25.0 bits (52), Expect = 9.1 Identities = 16/43 (37%), Positives = 21/43 (48%), Gaps = 1/43 (2%) Frame = -3 Query: 383 SVFRAPALGMYPSGVSAWQG-SXXQRRKIQSKTREFSSETGPE 258 S++ A Y S V Q S +K+Q+K E SET PE Sbjct: 81 SIYSAAQENAYRSSVKLIQEYSEKVHKKLQAKLLENPSETSPE 123 >SPBC18E5.10 |||iron sulfur cluster assembly protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.0 bits (52), Expect = 9.1 Identities = 12/25 (48%), Positives = 13/25 (52%) Frame = -1 Query: 400 RQRLGFPYSEHRLSVCTPVESRRGR 326 R R GFP E LS+ S RGR Sbjct: 81 RGRYGFPTGEKMLSLYKATSSERGR 105 >SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1078 Score = 25.0 bits (52), Expect = 9.1 Identities = 13/53 (24%), Positives = 28/53 (52%) Frame = +1 Query: 385 NLSAVDMNELFSIPKDFWLQEVSYRFFTTTRTLPSSDVKQSCAAG*ESSLIQS 543 N+S ++ E+F I ++W + V+ + + + +P S++ +SLI S Sbjct: 344 NISRINEREIFKICLEYWSKLVA-QLYEEMQQIPMSEMNPLLNLSSPTSLISS 395 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,473,573 Number of Sequences: 5004 Number of extensions: 48827 Number of successful extensions: 129 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 129 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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