BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0130 (743 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 28 1.6 SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3... 27 2.8 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.8 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 3.7 SPAC1002.15c |pmc5|med6|mediator complex subunit Pmc5 |Schizosac... 27 3.7 SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 27 3.7 SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 26 6.5 SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces ... 25 8.6 SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual 25 8.6 SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 25 8.6 SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 25 8.6 SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces ... 25 8.6 >SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 27.9 bits (59), Expect = 1.6 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Frame = -2 Query: 238 IGAAITIGDVKSNLQINLFGREVGPAVNNFLEKIPVYLTDYAAE-VSRVLEYVAQLV 71 + AAIT K + + F +E G V E+I + DY AE S++LE QL+ Sbjct: 94 VNAAITYCKDKDTIDESEFDKETGVGVVLTPEQIEQLVGDYVAENKSKILEQRYQLL 150 >SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 449 Score = 27.1 bits (57), Expect = 2.8 Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 1/40 (2%) Frame = -2 Query: 229 AITIGDVKSNLQINLFGREVGPAVNNFL-EKIPVYLTDYA 113 AITIGD + L + +++GP V +F + +P +LT ++ Sbjct: 178 AITIGDNSTLLYTSWLFQKIGPPVLSFSDDDVPGFLTHFS 217 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = -1 Query: 200 PPNQSLRTRSWTSRQQLPREDPCLPDGLRSRGQPCSGVRRP 78 PP S+ S S +P + P +P L QP + V+ P Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSP 184 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 26.6 bits (56), Expect = 3.7 Identities = 29/126 (23%), Positives = 52/126 (41%), Gaps = 6/126 (4%) Frame = +2 Query: 209 DIANSYCSSDVTDNDVSARETEAVASATTFSRASPPEAKDLRTRLKMRISPSAEP--TDA 382 D S S ++ + S++ + + S++TFS A+P +S S+ P + + Sbjct: 168 DPLTSSTFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSS 227 Query: 383 ETLGTARTKLSRVPT*LTRRK--CRELKSSALTSVTRPEKLMMSM--HPEGSRSQRTVTA 550 TL ++ S +P+ + L SS+ +S S+ S S T T+ Sbjct: 228 STLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTS 287 Query: 551 ERISRS 568 IS S Sbjct: 288 STISSS 293 >SPAC1002.15c |pmc5|med6|mediator complex subunit Pmc5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 26.6 bits (56), Expect = 3.7 Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 5/36 (13%) Frame = +1 Query: 283 LSNDVLKGKSSGSERPQD-----AAENADFSFSGTD 375 LSND L+ S + P D + ENAD+SFS D Sbjct: 160 LSNDNLEVDHSNTNEPADENKNQSIENADYSFSPED 195 >SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1|||Manual Length = 753 Score = 26.6 bits (56), Expect = 3.7 Identities = 15/30 (50%), Positives = 18/30 (60%) Frame = +2 Query: 221 SYCSSDVTDNDVSARETEAVASATTFSRAS 310 SY SD TD DVS ++E S+ SRAS Sbjct: 58 SYRQSDDTDGDVSESQSEYRLSSGRRSRAS 87 >SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 744 Score = 25.8 bits (54), Expect = 6.5 Identities = 18/61 (29%), Positives = 30/61 (49%) Frame = +2 Query: 347 MRISPSAEPTDAETLGTARTKLSRVPT*LTRRKCRELKSSALTSVTRPEKLMMSMHPEGS 526 +R + + E TD E + T +T LS + + +S +S TRP + + HP+G Sbjct: 474 LRKASTLETTDQEQIDTWKTTLSEKLESIWKALYIN-ESLEASSKTRPPFISIISHPKGM 532 Query: 527 R 529 R Sbjct: 533 R 533 >SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 846 Score = 25.4 bits (53), Expect = 8.6 Identities = 15/36 (41%), Positives = 17/36 (47%), Gaps = 1/36 (2%) Frame = +1 Query: 265 GDGSGSLSNDVLKGKSSGSERPQ-DAAENADFSFSG 369 G GSG L ND L+ K S P +NA S G Sbjct: 117 GPGSGKLLNDTLQSKISSIHMPHVQQGDNAVVSSVG 152 >SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual Length = 478 Score = 25.4 bits (53), Expect = 8.6 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +2 Query: 551 ERISRSQQFTDFIEQV*DHDVKDA 622 E +SR QQF D + + ++KDA Sbjct: 63 EEVSRRQQFVDKLRTILSTEIKDA 86 >SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1935 Score = 25.4 bits (53), Expect = 8.6 Identities = 12/41 (29%), Positives = 24/41 (58%) Frame = -2 Query: 187 LFGREVGPAVNNFLEKIPVYLTDYAAEVSRVLEYVAQLVIN 65 + R++GP V F+ K+P L + ++ + + V +LV+N Sbjct: 972 IHNRKMGPTVKKFISKLP--LLNINVDLLPLTKNVLRLVLN 1010 >SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation factor eIF2 beta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 25.4 bits (53), Expect = 8.6 Identities = 22/111 (19%), Positives = 48/111 (43%), Gaps = 1/111 (0%) Frame = +2 Query: 230 SSDVTDNDVSARETEAVASATTFSRASPPEAKDLRTRL-KMRISPSAEPTDAETLGTART 406 S+ TD D S R++ A T + E KD+ + + K + S + + E T Sbjct: 46 SAGATDEDDSERKSSAKDLTTPVTEGEVDELKDMFSSMKKKKKSKKSSASAEEQTEDITT 105 Query: 407 KLSRVPT*LTRRKCRELKSSALTSVTRPEKLMMSMHPEGSRSQRTVTAERI 559 + + ++K ++ KS+ L++ + + + S++T +E + Sbjct: 106 ESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDATSDLSKQTFDSENM 156 >SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 965 Score = 25.4 bits (53), Expect = 8.6 Identities = 11/39 (28%), Positives = 20/39 (51%) Frame = +2 Query: 461 SSALTSVTRPEKLMMSMHPEGSRSQRTVTAERISRSQQF 577 S + + PE + S+HP SR + ++ ++R QF Sbjct: 189 SLEIAPLISPESQLSSLHPSSSR-RHLISTPHVNRGTQF 226 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,737,889 Number of Sequences: 5004 Number of extensions: 49480 Number of successful extensions: 177 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 174 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 353266144 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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