Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0126
(518 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 122 1e-28
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 85 4e-17
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 85 4e-17
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 85 4e-17
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 72 3e-13
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 45 4e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 45 4e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 43 1e-04
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 30 1.1
At5g17300.1 68418.m02026 myb family transcription factor similar... 28 3.3
At3g24515.1 68416.m03077 ubiquitin-conjugating enzyme, putative ... 28 3.3
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 3.3
At5g41450.1 68418.m05034 zinc finger (C3HC4-type RING finger) fa... 28 4.3
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 4.3
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 27 7.6
At2g44700.1 68415.m05563 kelch repeat-containing F-box family pr... 27 7.6
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 122 bits (295), Expect = 1e-28
Identities = 61/82 (74%), Positives = 67/82 (81%), Gaps = 1/82 (1%)
Frame = +3
Query: 12 KLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDH-ACIPIKKSDPVV 188
KLVEGLKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL++D I KSDPVV
Sbjct: 503 KLVEGLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPVV 562
Query: 189 SYRETVAEESDQLCLSKSPNKH 254
S+RETV + S + +SKSPNKH
Sbjct: 563 SFRETVCDRSTRTVMSKSPNKH 584
Score = 121 bits (292), Expect = 3e-28
Identities = 55/91 (60%), Positives = 71/91 (78%)
Frame = +2
Query: 236 KVAQQAHRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFG 415
K + +RL+M+A+PM +GL E ID+GR+ PRDD K R++ L E++ +D A+KIW FG
Sbjct: 579 KSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFG 638
Query: 416 PEGTGPNILVDCSKGVQYLNEIKDSVVAGFQ 508
PE TGPN++VD KGVQYLNEIKDSVVAGFQ
Sbjct: 639 PETTGPNMVVDMCKGVQYLNEIKDSVVAGFQ 669
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 84.6 bits (200), Expect = 4e-17
Identities = 38/80 (47%), Positives = 57/80 (71%)
Frame = +3
Query: 12 KLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVS 191
K+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS
Sbjct: 602 KMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVS 661
Query: 192 YRETVAEESDQLCLSKSPNK 251
+ ETV E S C +++PNK
Sbjct: 662 FCETVVESSSMKCFAETPNK 681
Score = 61.3 bits (142), Expect = 4e-10
Identities = 33/89 (37%), Positives = 51/89 (57%), Gaps = 4/89 (4%)
Frame = +2
Query: 254 HRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGP 433
++L M A+P+ GL EDI+ G V+ + + KY++D+ AR IW FGP+ G
Sbjct: 683 NKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAFGPDKQGT 742
Query: 434 NILVDCSKGVQ----YLNEIKDSVVAGFQ 508
NIL+D + + + +KDS+V GFQ
Sbjct: 743 NILLDDTLPTEVDRNLMMGVKDSIVQGFQ 771
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 84.6 bits (200), Expect = 4e-17
Identities = 38/80 (47%), Positives = 57/80 (71%)
Frame = +3
Query: 12 KLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVS 191
K+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS
Sbjct: 616 KMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVS 675
Query: 192 YRETVAEESDQLCLSKSPNK 251
+ ETV E S C +++PNK
Sbjct: 676 FCETVVESSSMKCFAETPNK 695
Score = 66.9 bits (156), Expect = 8e-12
Identities = 34/89 (38%), Positives = 53/89 (59%), Gaps = 4/89 (4%)
Frame = +2
Query: 254 HRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGP 433
+++ M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GP
Sbjct: 697 NKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGP 756
Query: 434 NILVDCSKGVQ----YLNEIKDSVVAGFQ 508
NIL+D + + + +KDS+V GFQ
Sbjct: 757 NILLDDTLPTEVDRNLMMAVKDSIVQGFQ 785
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 84.6 bits (200), Expect = 4e-17
Identities = 38/80 (47%), Positives = 57/80 (71%)
Frame = +3
Query: 12 KLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVS 191
K+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS
Sbjct: 616 KMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVS 675
Query: 192 YRETVAEESDQLCLSKSPNK 251
+ ETV E S C +++PNK
Sbjct: 676 FCETVVESSSMKCFAETPNK 695
Score = 66.9 bits (156), Expect = 8e-12
Identities = 34/89 (38%), Positives = 53/89 (59%), Gaps = 4/89 (4%)
Frame = +2
Query: 254 HRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGP 433
+++ M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GP
Sbjct: 697 NKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGP 756
Query: 434 NILVDCSKGVQ----YLNEIKDSVVAGFQ 508
NIL+D + + + +KDS+V GFQ
Sbjct: 757 NILLDDTLPTEVDRNLMMAVKDSIVQGFQ 785
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 71.7 bits (168), Expect = 3e-13
Identities = 32/71 (45%), Positives = 49/71 (69%)
Frame = +3
Query: 15 LVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSY 194
L++GL+ L ++DP V+ GEH++A AGE+HLE C+KDL+E A + ++ S P+VSY
Sbjct: 554 LMKGLRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLKERFAKVNLEVSPPLVSY 613
Query: 195 RETVAEESDQL 227
RET+ + L
Sbjct: 614 RETIEGDGSNL 624
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 44.8 bits (101), Expect = 4e-05
Identities = 21/68 (30%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +3
Query: 21 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYR 197
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 198 ETVAEESD 221
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 44.8 bits (101), Expect = 4e-05
Identities = 21/68 (30%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +3
Query: 21 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYR 197
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 198 ETVAEESD 221
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 43.2 bits (97), Expect = 1e-04
Identities = 23/68 (33%), Positives = 39/68 (57%), Gaps = 1/68 (1%)
Frame = +3
Query: 12 KLVEGLKRLAKSDPMVQCI-NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVV 188
K+ GL +LA+ DP +EE + ++ G GELHLEI + L+ + + P V
Sbjct: 509 KMATGLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRLKREFK-VEANVGAPQV 567
Query: 189 SYRETVAE 212
+YRE++++
Sbjct: 568 NYRESISK 575
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 29.9 bits (64), Expect = 1.1
Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = -1
Query: 260 DGVLVGRL*ETELVRFL-SYGLTVRHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 87
DG+ + + ET R + G TV+ DRV W +++ +++TD +EF N+
Sbjct: 328 DGLRISDVPETPSARETHTRGGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At5g17300.1 68418.m02026 myb family transcription factor similar to
CCA1 [Arabidopsis thaliana] GI:4090569; contains Pfam
profile PF00249: Myb-like DNA-binding domain
Length = 387
Score = 28.3 bits (60), Expect = 3.3
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -2
Query: 217 DSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPAT 92
D+ + S T GS+ L + + S +RSL +S S PA T
Sbjct: 154 DTQSPTSVLSTVGSEALCSLDSSSPNRSLSPVSSASPPAALT 195
>At3g24515.1 68416.m03077 ubiquitin-conjugating enzyme, putative
similar to Ubiquitin-conjugating enzyme E2
(Ubiquitin-protein ligase) (Ubiquitin carrier protein)
from {Xenopus laevis} SP|P51669, {Schizosaccharomyces
pombe} SP|P46595, {Caenorhabditis elegans} SP|P35129;
contains Pfam profile PF00179: Ubiquitin-conjugating
enzyme
Length = 409
Score = 28.3 bits (60), Expect = 3.3
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +2
Query: 221 PALSLKVAQQAHRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKY 373
P+L++ + RL + DGL ++ R F +AR +TEKY
Sbjct: 113 PSLNISTVLTSMRLLLSEPNPDDGLMCEVSREYKYNRQTFDYKAREMTEKY 163
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3
Query: 66 INEESGEHIVAGAGELHLEICLKDLEEDH 152
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At5g41450.1 68418.m05034 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 164
Score = 27.9 bits (59), Expect = 4.3
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +2
Query: 236 KVAQQAHRLFMKAQPMPDGLPEDIDEGRVN-PRDDFKTRARYLTEKYEYDVTEARKI 403
++A++ H+ + P PD L +DI+ G V P+ Y+T +E + E + I
Sbjct: 48 EIARETHQ---NSHPPPDQLQQDIETGHVTLPQPQQNIAVGYMTWIHETTILEFKDI 101
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 99 GAGELHLEICLKDLEEDHACIPIKK 173
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 27.1 bits (57), Expect = 7.6
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +1
Query: 319 RESPR*LQDSRSVSYRKVRI*CYRSP*DLV 408
+E+PR DSRS S+R R C P +LV
Sbjct: 223 KETPRLSLDSRSNSFRSARSSCSPEPQELV 252
>At2g44700.1 68415.m05563 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif;
Length = 368
Score = 27.1 bits (57), Expect = 7.6
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = +2
Query: 302 DIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWC 409
D+D R ++ + ++ EK +V+E+++IWC
Sbjct: 294 DLDNARSVGIGEYYGKLAFIWEKPSLNVSESKEIWC 329
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,369,485
Number of Sequences: 28952
Number of extensions: 270972
Number of successful extensions: 904
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 873
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 899
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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