BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0101
(776 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC5H10.01 |||DUF1445 family protein|Schizosaccharomyces pombe|... 27 2.3
SPAC29A4.13 |||urease accessory protein UreF|Schizosaccharomyces... 27 2.3
SPAC29B12.10c |||OPT oligopeptide transporter family|Schizosacch... 27 2.3
SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 26 5.2
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 26 5.2
SPCC4B3.05c |hem12||uroporphyrinogen decarboxylase |Schizosaccha... 26 6.9
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 25 9.2
SPBC11G11.04 |trs20||TRAPP complex subunit Trs20 |Schizosaccharo... 25 9.2
>SPAC5H10.01 |||DUF1445 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 301
Score = 27.5 bits (58), Expect = 2.3
Identities = 23/76 (30%), Positives = 32/76 (42%)
Frame = +2
Query: 293 YGESVYCGWSPDPNTKIKARDCVDKWYSEINEFSFGKEPEVLNCGHFTQIIWRSTSELGI 472
+GE V GW D KI +DC + EF G+ P CG Q + S+L
Sbjct: 226 HGEPVAWGW--DGAKKIGVKDCGKPDFGVPVEFKEGEIPIFWGCGVTPQNV-VMMSKLPE 282
Query: 473 GSAKSKTGKLYVSQTT 520
K G +Y++ T
Sbjct: 283 PVYSHKPGYMYLTDLT 298
>SPAC29A4.13 |||urease accessory protein UreF|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 235
Score = 27.5 bits (58), Expect = 2.3
Identities = 18/58 (31%), Positives = 30/58 (51%)
Frame = +2
Query: 545 YSLKMSYPLGLCSSALATIVLLENRTTIWLLLHQTEYLLEDPSLVLPLRLQVKEVART 718
Y + + +CS+A+ VL + L QTE LL D S L L +Q+++ A++
Sbjct: 158 YLFLLGHAKSICSAAVRLDVLTSFQYVSTLAHPQTESLLRDSS-QLALNMQLEDTAQS 214
>SPAC29B12.10c |||OPT oligopeptide transporter
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 851
Score = 27.5 bits (58), Expect = 2.3
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -3
Query: 558 IFNE*PAVISWWIVVCET*SFPVLLFALPIP 466
++N P WW V+C T VL+F + +P
Sbjct: 394 VYNSNPLASPWW-VICNTFGSVVLIFWIVVP 423
>SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1125
Score = 26.2 bits (55), Expect = 5.2
Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 1/59 (1%)
Frame = +2
Query: 374 SEINEFSFGKEPEVLNCGHFTQIIWRSTSELGIGSAKSKTGKL-YVSQTTIHQEITAGY 547
SE + + ++ V N + +I + T++ +GS K GKL Y Q T+ +E T+ Y
Sbjct: 616 SENAKLAAQRDAAVENANYSRSLIQKKTTDETVGSLIEKVGKLEYEVQGTL-EEATSYY 673
>SPAC694.06c |mrc1||mediator of replication checkpoint 1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1019
Score = 26.2 bits (55), Expect = 5.2
Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 3/49 (6%)
Frame = +3
Query: 126 SKSNDFENEALEVHNEYRREHGV---SPLVINKEISKISQKWAEELLKE 263
+K D E E LE E RR+ ++ NK+ K WA +L++E
Sbjct: 661 NKDQDDE-EILEERRESRRDSKTFLSRTMLYNKDTGKADSAWASDLIEE 708
>SPCC4B3.05c |hem12||uroporphyrinogen decarboxylase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 355
Score = 25.8 bits (54), Expect = 6.9
Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +2
Query: 326 DPNTKIKARDCVDKWYSE-INEFSFGKEPEVLNCGH 430
DPN R+ ++ E I +F GK+ ++N GH
Sbjct: 296 DPNILYGTREIIEARTKEMIQDFGGGKQGYIINLGH 331
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 25.4 bits (53), Expect = 9.2
Identities = 16/65 (24%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +3
Query: 168 NEYRREHGVSPLVINKEISKISQKW---AEELLKETVSPTV*TKGMVKVSTADGHLTQIQ 338
+E+ E+G+S +INKE++ + +K L +E ++ + ++G + G+L I
Sbjct: 248 SEHVIENGISEDLINKEVNVVEEKLRPPVRLLTEEELNELLPSEGYAILEPPPGYLESIH 307
Query: 339 RSKLE 353
L+
Sbjct: 308 PELLQ 312
>SPBC11G11.04 |trs20||TRAPP complex subunit Trs20
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 136
Score = 25.4 bits (53), Expect = 9.2
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -2
Query: 166 WTSNASFSKSFDLEVNSLLIVLYVTSIPLNYL 71
WTSNA + K+ D + + + I YVT + ++
Sbjct: 52 WTSNAFYMKTID-QFHEMYISAYVTPSNMRFM 82
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,031,021
Number of Sequences: 5004
Number of extensions: 60727
Number of successful extensions: 169
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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