Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0100
(756 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g01380.1 68418.m00051 expressed protein 27 0.39
At3g57640.1 68416.m06422 protein kinase family protein contains ... 32 0.47
At5g47370.1 68418.m05838 homeobox-leucine zipper protein 2 (HAT2... 31 0.62
At2g19110.1 68415.m02231 ATPase E1-E2 type family protein / halo... 25 1.7
At1g64050.1 68414.m07255 expressed protein 25 2.3
At5g63720.1 68418.m07998 hypothetical protein 25 2.3
At5g63520.1 68418.m07973 F-box family protein similar to unknown... 29 2.5
At2g18380.1 68415.m02141 zinc finger (GATA type) family protein 27 2.5
At1g21326.1 68414.m02666 VQ motif-containing protein contains PF... 25 3.2
At2g36710.1 68415.m04504 pectinesterase family protein contains ... 26 4.0
At5g51300.2 68418.m06360 splicing factor-related contains simila... 28 5.8
At5g51300.1 68418.m06359 splicing factor-related contains simila... 28 5.8
At5g28590.1 68418.m03487 DNA-binding protein-related contains si... 28 5.8
At5g11500.1 68418.m01342 expressed protein contains Pfam profile... 28 7.7
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 23 8.0
At5g51800.1 68418.m06423 expressed protein 25 8.1
At4g37270.1 68417.m05275 cadmium/zinc-transporting ATPase, putat... 23 8.2
At1g44770.1 68414.m05129 expressed protein 25 9.0
>At5g01380.1 68418.m00051 expressed protein
Length = 323
Score = 27.5 bits (58), Expect(2) = 0.39
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -1
Query: 474 KKNIFYNHHHHHQ 436
++N F +HHHHHQ
Sbjct: 3 RRNPFQHHHHHHQ 15
Score = 23.4 bits (48), Expect(2) = 0.39
Identities = 6/13 (46%), Positives = 8/13 (61%)
Frame = -1
Query: 459 YNHHHHHQPISLH 421
+ HHHHH + H
Sbjct: 7 FQHHHHHHQLHHH 19
>At3g57640.1 68416.m06422 protein kinase family protein contains
similarity to protein kinases
Length = 356
Score = 31.9 bits (69), Expect = 0.47
Identities = 15/35 (42%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
Frame = -1
Query: 456 NHHHHHQPISLHCWIYVSLRHNERSFA--FRISSI 358
NHHHHH+ + + W Y HN +F ISSI
Sbjct: 83 NHHHHHKLLLIKKWRYRFSEHNGGNFCRDIAISSI 117
>At5g47370.1 68418.m05838 homeobox-leucine zipper protein 2 (HAT2) /
HD-ZIP protein 2 identical to homeobox-leucine zipper
protein HAT2 (HD-ZIP protein 2) [Arabidopsis thaliana]
SP:P46601; contains Pfam profiles PF04618: HD-ZIP
protein N terminus, PF02183: Homeobox associated leucine
zipper, PF00046: Homeobox domain
Length = 283
Score = 31.5 bits (68), Expect = 0.62
Identities = 8/15 (53%), Positives = 14/15 (93%)
Frame = -1
Query: 456 NHHHHHQPISLHCWI 412
NHHH+H+P+S++ W+
Sbjct: 250 NHHHNHRPVSINPWV 264
>At2g19110.1 68415.m02231 ATPase E1-E2 type family protein / haloacid
dehalogenase-like hydrolase family protein /
heavy-metal-associated domain-containing protein similar
to cadmium efflux pump protein from Geobacillus
stearothermophilus [GI:16753175], cadmium resistance
protein B from Staphylococcus aureus [GI:14020985];
T20K24.13 has been merged with T20K24.12 per suggestion
of Dr. Kristian Axelsen (axe@biobase.dk)
Length = 1172
Score = 25.0 bits (52), Expect(2) = 1.7
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = -1
Query: 489 CK*Y*KKNIFYNHHHHH 439
C+ Y K+ + HHHHH
Sbjct: 1147 CRSYAKELCSHRHHHHH 1163
Score = 23.4 bits (48), Expect(2) = 1.7
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = -1
Query: 459 YNHHHHHQPIS 427
++HHHHH +S
Sbjct: 1161 HHHHHHHHHVS 1171
>At1g64050.1 68414.m07255 expressed protein
Length = 668
Score = 24.6 bits (51), Expect(2) = 2.3
Identities = 11/33 (33%), Positives = 16/33 (48%)
Frame = -1
Query: 456 NHHHHHQPISLHCWIYVSLRHNERSFAFRISSI 358
+HHHHH P ++ + +RSF S I
Sbjct: 593 HHHHHHHPKPSKRLKLSTMENKKRSFPSSSSPI 625
Score = 23.4 bits (48), Expect(2) = 2.3
Identities = 6/7 (85%), Positives = 7/7 (100%)
Frame = -1
Query: 459 YNHHHHH 439
+NHHHHH
Sbjct: 591 HNHHHHH 597
>At5g63720.1 68418.m07998 hypothetical protein
Length = 492
Score = 25.4 bits (53), Expect(2) = 2.3
Identities = 7/9 (77%), Positives = 9/9 (100%)
Frame = -1
Query: 465 IFYNHHHHH 439
IF++HHHHH
Sbjct: 367 IFHHHHHHH 375
Score = 22.6 bits (46), Expect(2) = 2.3
Identities = 6/15 (40%), Positives = 8/15 (53%)
Frame = -1
Query: 459 YNHHHHHQPISLHCW 415
++HHHHH W
Sbjct: 375 HHHHHHHDKEKPSAW 389
>At5g63520.1 68418.m07973 F-box family protein similar to unknown
protein (pir||S76033) ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 572
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/56 (23%), Positives = 28/56 (50%)
Frame = +2
Query: 170 VELDRLDIIPWSTLEHSA*HNLQIKTTDC*YFLASCIQRRSSNSCTTLFLQGNIIS 337
++ D D++P + + HN+ ++ + ASC+ R S+ C + + +IS
Sbjct: 23 MKTDMADMVPIAAMNEDLLHNILLRLPAKSFAFASCVNRFWSSVCNRILSRPKMIS 78
>At2g18380.1 68415.m02141 zinc finger (GATA type) family protein
Length = 207
Score = 27.5 bits (58), Expect(2) = 2.5
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = -1
Query: 462 FYNHHHHHQPISLHCWIY 409
+Y+HHHHH S W +
Sbjct: 158 YYSHHHHHYASSSPSWAH 175
Score = 20.6 bits (41), Expect(2) = 2.5
Identities = 5/10 (50%), Positives = 8/10 (80%)
Frame = -1
Query: 468 NIFYNHHHHH 439
N + +HHHH+
Sbjct: 157 NYYSHHHHHY 166
>At1g21326.1 68414.m02666 VQ motif-containing protein contains
PF05678: VQ motif
Length = 239
Score = 25.4 bits (53), Expect(2) = 3.2
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = -1
Query: 459 YNHHHHHQ 436
Y+HHHHHQ
Sbjct: 153 YHHHHHHQ 160
Score = 22.2 bits (45), Expect(2) = 3.2
Identities = 5/8 (62%), Positives = 8/8 (100%)
Frame = -1
Query: 462 FYNHHHHH 439
+Y++HHHH
Sbjct: 150 YYHYHHHH 157
>At2g36710.1 68415.m04504 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 407
Score = 25.8 bits (54), Expect(2) = 4.0
Identities = 11/27 (40%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = -1
Query: 459 YNHHHHHQPISLHCWIYVS-LRHNERS 382
++H+HHH+PI C + S LRH ++
Sbjct: 62 HHHYHHHEPIKC-CEKWTSRLRHQYKT 87
Score = 21.4 bits (43), Expect(2) = 4.0
Identities = 6/12 (50%), Positives = 8/12 (66%)
Frame = -1
Query: 474 KKNIFYNHHHHH 439
K + F+ H HHH
Sbjct: 43 KYSTFFGHKHHH 54
>At5g51300.2 68418.m06360 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -3
Query: 754 RNSSSEISNGTAEAIAEVDAISCHPTQLNALLCGNGLCRT 635
+NS S NG++ I + + S P L LL NG+ +T
Sbjct: 36 QNSESVALNGSSTPIPDTNGSSAKPELLRPLLSENGVSKT 75
>At5g51300.1 68418.m06359 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -3
Query: 754 RNSSSEISNGTAEAIAEVDAISCHPTQLNALLCGNGLCRT 635
+NS S NG++ I + + S P L LL NG+ +T
Sbjct: 36 QNSESVALNGSSTPIPDTNGSSAKPELLRPLLSENGVSKT 75
>At5g28590.1 68418.m03487 DNA-binding protein-related contains
similarity to DNA-binding proteins
Length = 216
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Frame = +2
Query: 479 YYLHLNRFKRNTLYTYVSL*FLRWCMTPLVASVPP---LSVSTILTTPKRRLAIC 634
YY LNR K T+ V+L C TP + ++ P ++ +L T + + +C
Sbjct: 5 YYKRLNRLKSPTIRFSVALSKTGQCFTPHIVNITPGEDVAEKIVLFTQQSKHQLC 59
>At5g11500.1 68418.m01342 expressed protein contains Pfam profile
PF05670: Domain of unknown function (DUF814)
Length = 215
Score = 27.9 bits (59), Expect = 7.7
Identities = 13/39 (33%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +1
Query: 142 WNRLEQNLRRRIGQAGYHTMVYTGTFRVTQLTNQ-NNRL 255
W+ L++ +GQ G+H T RV + N+ NRL
Sbjct: 94 WSNLKKTASMDVGQVGFHNSKMVRTIRVEKRVNEIVNRL 132
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 23.4 bits (48), Expect(2) = 8.0
Identities = 6/12 (50%), Positives = 10/12 (83%)
Frame = -1
Query: 459 YNHHHHHQPISL 424
++HHHHH ++L
Sbjct: 19 HHHHHHHHHLAL 30
Score = 22.6 bits (46), Expect(2) = 8.0
Identities = 5/11 (45%), Positives = 10/11 (90%)
Frame = -1
Query: 471 KNIFYNHHHHH 439
+++ ++HHHHH
Sbjct: 13 RDVRHHHHHHH 23
>At5g51800.1 68418.m06423 expressed protein
Length = 972
Score = 24.6 bits (51), Expect(2) = 8.1
Identities = 6/11 (54%), Positives = 10/11 (90%)
Frame = -1
Query: 468 NIFYNHHHHHQ 436
++ ++HHHHHQ
Sbjct: 33 SLLHHHHHHHQ 43
Score = 21.4 bits (43), Expect(2) = 8.1
Identities = 7/17 (41%), Positives = 10/17 (58%)
Frame = -1
Query: 456 NHHHHHQPISLHCWIYV 406
+HHHHH L I++
Sbjct: 36 HHHHHHHQSFLPTPIFI 52
>At4g37270.1 68417.m05275 cadmium/zinc-transporting ATPase, putative
(HMA1) contains InterPro accession IPR001757: ATPase,
E1-E2 type; identical to Potential
cadmium/zinc-transporting ATPase HMA1 (EC 3.6.3.3) (EC
3.6.3.5) (Swiss-Prot:Q9M3H5) [Arabidopsis thaliana];
identical to cDNA putative transcription factor (MYB73)
mRNA, partial cds GI:3941503
Length = 819
Score = 23.4 bits (48), Expect(2) = 8.2
Identities = 6/7 (85%), Positives = 7/7 (100%)
Frame = -1
Query: 459 YNHHHHH 439
+NHHHHH
Sbjct: 80 HNHHHHH 86
Score = 22.6 bits (46), Expect(2) = 8.2
Identities = 6/7 (85%), Positives = 7/7 (100%)
Frame = -1
Query: 456 NHHHHHQ 436
+HHHHHQ
Sbjct: 82 HHHHHHQ 88
>At1g44770.1 68414.m05129 expressed protein
Length = 271
Score = 25.4 bits (53), Expect(2) = 9.0
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = -1
Query: 459 YNHHHHHQ 436
Y+HHHHHQ
Sbjct: 27 YHHHHHHQ 34
Score = 20.6 bits (41), Expect(2) = 9.0
Identities = 6/11 (54%), Positives = 6/11 (54%)
Frame = -1
Query: 453 HHHHHQPISLH 421
HHHHH H
Sbjct: 28 HHHHHHQDENH 38
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,282,594
Number of Sequences: 28952
Number of extensions: 328086
Number of successful extensions: 1270
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1043
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1206
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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