Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0069
(791 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 60 3e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 55 1e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 45 1e-05
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 39 7e-04
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 33 0.062
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 32 0.11
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 31 0.25
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 29 0.58
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 29 1.0
SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharo... 27 3.1
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 4.1
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 27 4.1
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 26 7.1
SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 9.4
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 60.5 bits (140), Expect = 3e-10
Identities = 22/59 (37%), Positives = 42/59 (71%)
Frame = +3
Query: 249 SFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMT 425
+F+++D++GNGYIT L +L +L ++LS ++ +I E DTDG G ++++EF +++
Sbjct: 90 AFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVIS 148
Score = 56.4 bits (130), Expect = 4e-09
Identities = 27/69 (39%), Positives = 46/69 (66%)
Frame = +3
Query: 249 SFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMTG 428
+F L+DR+ +G IT++ L ++ +L + A+L +I E+D DG+GT+DF EF+ MM
Sbjct: 17 AFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMM-- 74
Query: 429 D*AQRSKDS 455
A++ KD+
Sbjct: 75 --ARKMKDT 81
Score = 48.8 bits (111), Expect = 9e-07
Identities = 25/84 (29%), Positives = 50/84 (59%)
Frame = +1
Query: 1 KMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDG 180
++A R+AF +FD + G I ++ ++ ++GQ +ELQ +I+E D + +G I+F
Sbjct: 10 QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTE 69
Query: 181 FCNIASHFLEEEDAEAMQQELKEA 252
F + + +++ D E +E++EA
Sbjct: 70 FLTMMARKMKDTDNE---EEVREA 90
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/62 (32%), Positives = 38/62 (61%)
Frame = +1
Query: 13 LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNI 192
+R+AF++FD +GYI V +++ +L ++G+ E+ +I E D + G IN++ F +
Sbjct: 87 VREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRV 146
Query: 193 AS 198
S
Sbjct: 147 IS 148
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.2 bits (127), Expect = 1e-08
Identities = 22/55 (40%), Positives = 35/55 (63%)
Frame = +1
Query: 19 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGF 183
KAF++FD SGYI+ K + + T+G+ D+E+Q ++ E DP NSG ++ F
Sbjct: 82 KAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
Score = 46.4 bits (105), Expect = 5e-06
Identities = 17/57 (29%), Positives = 38/57 (66%)
Frame = +3
Query: 246 RSFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFME 416
++FR++D++ +GYI T+ + + L +KLS+ ++ ++ E D SG+ D+ +F++
Sbjct: 82 KAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 45.2 bits (102), Expect = 1e-05
Identities = 19/60 (31%), Positives = 36/60 (60%)
Frame = +1
Query: 13 LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNI 192
+++AF++FD ++G I + + + + + DD EL+A+I+E D + G+IN F I
Sbjct: 112 IKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAI 171
Score = 42.3 bits (95), Expect = 8e-05
Identities = 17/60 (28%), Positives = 35/60 (58%)
Frame = +3
Query: 243 ERSFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 422
+R+F L+D + G I+ L+ + L++ + + +L+ +I E D D G ++ EF+ +M
Sbjct: 113 KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIM 172
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 39.1 bits (87), Expect = 7e-04
Identities = 22/76 (28%), Positives = 42/76 (55%), Gaps = 9/76 (11%)
Frame = +3
Query: 228 HATRTER---SFRLYDREGNGYITTSTLKEILAAL------DDKLSNADLDGIIAEIDTD 380
H + E+ +F++YD + +GYI+ L +L + +D+L +D I E+D D
Sbjct: 88 HGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQI-VDKTIMEVDKD 146
Query: 381 GSGTVDFDEFMEMMTG 428
G + F+EF ++++G
Sbjct: 147 RDGKISFEEFKDIVSG 162
Score = 27.5 bits (58), Expect = 2.3
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +3
Query: 336 SNADLDGIIAEIDTDGSGTVDFDEFMEMMT 425
SN + + +D DG G VDF EF+ ++
Sbjct: 54 SNPLASRLFSVVDEDGGGDVDFQEFINSLS 83
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 32.7 bits (71), Expect = 0.062
Identities = 17/61 (27%), Positives = 30/61 (49%)
Frame = +1
Query: 19 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIAS 198
+AF +FD T GYID + +G+ +LQ ++D +GK++ + F +
Sbjct: 16 EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREEFAELWI 73
Query: 199 H 201
H
Sbjct: 74 H 74
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/59 (30%), Positives = 28/59 (47%)
Frame = +3
Query: 243 ERSFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEM 419
E +F L+D GYI L+ A L + L+ L ++ T+G V +EF E+
Sbjct: 15 EEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK--VSREEFAEL 71
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 31.9 bits (69), Expect = 0.11
Identities = 16/60 (26%), Positives = 35/60 (58%)
Frame = +3
Query: 243 ERSFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 422
+++F L+DR G G I +++ ++L A + A+ I EI++ VD ++F++++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQVL 64
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 30.7 bits (66), Expect = 0.25
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = +3
Query: 276 NGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEF 410
NGY++ S +L + KLS+ L+ I D D G DFDEF
Sbjct: 20 NGYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGMFDFDEF 62
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 29.5 bits (63), Expect = 0.58
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +1
Query: 67 LKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHF 204
+ +ST+ +T + DDS L+ +ID+ PE GK NI++HF
Sbjct: 12 ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGKPK----ANISTHF 52
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 28.7 bits (61), Expect = 1.0
Identities = 17/62 (27%), Positives = 28/62 (45%)
Frame = +1
Query: 7 AMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFC 186
A F +FD K+GYID + L+ + + +L D +N+G I++D
Sbjct: 63 AFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEML 122
Query: 187 NI 192
I
Sbjct: 123 RI 124
>SPAC7D4.06c |alg3||dolichol-P-Man dependent
alpha|Schizosaccharomyces pombe|chr 1|||Manual
Length = 406
Score = 27.1 bits (57), Expect = 3.1
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +3
Query: 654 FEFVPKSYFWNNSLVTSFFFLHCNHLMLIFITH 752
+ F+P+S F + S TS FLH L+ H
Sbjct: 254 WRFIPRSIFESTSFSTSILFLHVALLVAFTCKH 286
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 26.6 bits (56), Expect = 4.1
Identities = 18/57 (31%), Positives = 22/57 (38%)
Frame = +3
Query: 252 FRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 422
F D E GYI A L + +L I +DT G +D DEF M
Sbjct: 308 FSKIDNENKGYIVGGEAVPFFMA--SHLDSEELARIWDTVDTQDRGYIDKDEFAVAM 362
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 26.6 bits (56), Expect = 4.1
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = +1
Query: 586 RFCLSCRNVLVIYVPASCRSRTYLNSFQRVIFGI 687
R CL+ R ++ +Y+ C + N F++++F I
Sbjct: 533 RICLNPRALVELYINYDCDRSSTTNVFEQLLFSI 566
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1183
Score = 25.8 bits (54), Expect = 7.1
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = +3
Query: 258 LYDREGNGYITT-STLKEILAALDDKLSNADLDGII-AEIDTDGSGTVDFDEFMEMMTGD 431
LYD+E GY+T + E++ + D+ N D + ++ + SG V E+
Sbjct: 1055 LYDKEKRGYVTLFPSGSEVVQFVPDRTYNIDSEYVVYLSLHESRSGRVYMSPLTEISPEH 1114
Query: 432 *AQRSKDS 455
A+ +K++
Sbjct: 1115 LARLAKNT 1122
>SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 9.4
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +3
Query: 267 REGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 374
RE GY+ T + D L+NA+L I+A+ID
Sbjct: 107 REETGYVGTVMDSTTVMYNDPGLTNANLKIILADID 142
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,051,219
Number of Sequences: 5004
Number of extensions: 59829
Number of successful extensions: 189
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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