BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0059 (727 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 33 0.031 SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schi... 29 0.51 SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Sc... 28 1.6 SPAC1F7.10 |||hydantoin racemase family |Schizosaccharomyces pom... 27 2.1 SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces po... 26 6.3 SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 26 6.3 >SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 33.5 bits (73), Expect = 0.031 Identities = 25/89 (28%), Positives = 39/89 (43%), Gaps = 3/89 (3%) Frame = -2 Query: 258 RRDYVYIFSGEVSGYDESVEVSSDSF---TVIVYREDGAASEGERRSHNRVDRQQQRFTD 88 RR+ EV +E +E D + E GA G+ R H +DRQ+Q Sbjct: 142 RRNQFLDIEAEVDEDEEELEDEEDEIGREDGFIEEEVGADYVGDDRRHRELDRQRQELQS 201 Query: 87 LRASPMKEVKYNIPFSQIETVRCSSSLVP 1 + A + E +Y + + +TV +S VP Sbjct: 202 VDAERLAE-EYREKYGRSQTVVGDTSNVP 229 >SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schizosaccharomyces pombe|chr 1|||Manual Length = 310 Score = 29.5 bits (63), Expect = 0.51 Identities = 16/47 (34%), Positives = 24/47 (51%) Frame = -2 Query: 339 LMNSAINRPH*RALARPSVYNFDXDSSRRDYVYIFSGEVSGYDESVE 199 ++++ IN P PSV N DS D +Y G + G DE++E Sbjct: 199 VVHARINNPDDHLRTHPSVNNLGKDSDHADKMY-NEGVILGEDEAME 244 >SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 27.9 bits (59), Expect = 1.6 Identities = 19/58 (32%), Positives = 34/58 (58%), Gaps = 1/58 (1%) Frame = +1 Query: 88 VSEPLLLSVNSIMTSSFSLARRTIFTIHN-HGETVAGNFNAFVIPAHLAAEDVNVIAS 258 VS+ L+ S++ I + SFSL + +F +HN GE++ + A ++++V IAS Sbjct: 316 VSDMLIQSLSIINSPSFSLG-KIVFLVHNIDGESLIDERFQSALAAIASSKNVYFIAS 372 >SPAC1F7.10 |||hydantoin racemase family |Schizosaccharomyces pombe|chr 1|||Manual Length = 238 Score = 27.5 bits (58), Expect = 2.1 Identities = 15/27 (55%), Positives = 18/27 (66%) Frame = -2 Query: 663 ET*RKNGAGIVQASALTATAGGIKVEI 583 ET RK GI+QAS LTA + G KV + Sbjct: 89 ETYRKPCTGIMQASILTALSLGRKVSV 115 >SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.8 bits (54), Expect = 6.3 Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%) Frame = +1 Query: 511 PDDANIVEVLHTTAGLIGYDYPLGDLDFY-PSGG 609 P D +I VLH TA +I + +DF+ P GG Sbjct: 178 PLDFSITIVLHLTAVVISIIFSFAYMDFFLPRGG 211 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 25.8 bits (54), Expect = 6.3 Identities = 17/72 (23%), Positives = 31/72 (43%) Frame = +1 Query: 28 SFNLGERDVVFHLFHRGSPQVSEPLLLSVNSIMTSSFSLARRTIFTIHNHGETVAGNFNA 207 S N G+ + H F+R S + + LL+ S + ++ F + F Sbjct: 545 SLNFGKISFISHEFYRVSKRFLDILLIWFESYLVEELDNSKSIFFLF-----KIYKVFEV 599 Query: 208 FVIPAHLAAEDV 243 FV+P +AE++ Sbjct: 600 FVVPHFASAEEL 611 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,777,915 Number of Sequences: 5004 Number of extensions: 52910 Number of successful extensions: 128 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 128 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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