BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0059
(727 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 33 0.031
SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schi... 29 0.51
SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Sc... 28 1.6
SPAC1F7.10 |||hydantoin racemase family |Schizosaccharomyces pom... 27 2.1
SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces po... 26 6.3
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 26 6.3
>SPAC23C4.19 |spt5||transcription elongation factor
Spt5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 990
Score = 33.5 bits (73), Expect = 0.031
Identities = 25/89 (28%), Positives = 39/89 (43%), Gaps = 3/89 (3%)
Frame = -2
Query: 258 RRDYVYIFSGEVSGYDESVEVSSDSF---TVIVYREDGAASEGERRSHNRVDRQQQRFTD 88
RR+ EV +E +E D + E GA G+ R H +DRQ+Q
Sbjct: 142 RRNQFLDIEAEVDEDEEELEDEEDEIGREDGFIEEEVGADYVGDDRRHRELDRQRQELQS 201
Query: 87 LRASPMKEVKYNIPFSQIETVRCSSSLVP 1
+ A + E +Y + + +TV +S VP
Sbjct: 202 VDAERLAE-EYREKYGRSQTVVGDTSNVP 229
>SPAC17A5.09c |||protein phosphatase regulatory subunit
Glc9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 310
Score = 29.5 bits (63), Expect = 0.51
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -2
Query: 339 LMNSAINRPH*RALARPSVYNFDXDSSRRDYVYIFSGEVSGYDESVE 199
++++ IN P PSV N DS D +Y G + G DE++E
Sbjct: 199 VVHARINNPDDHLRTHPSVNNLGKDSDHADKMY-NEGVILGEDEAME 244
>SPBC685.09 |orc2|orp2|origin recognition complex subunit
Orc2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 27.9 bits (59), Expect = 1.6
Identities = 19/58 (32%), Positives = 34/58 (58%), Gaps = 1/58 (1%)
Frame = +1
Query: 88 VSEPLLLSVNSIMTSSFSLARRTIFTIHN-HGETVAGNFNAFVIPAHLAAEDVNVIAS 258
VS+ L+ S++ I + SFSL + +F +HN GE++ + A ++++V IAS
Sbjct: 316 VSDMLIQSLSIINSPSFSLG-KIVFLVHNIDGESLIDERFQSALAAIASSKNVYFIAS 372
>SPAC1F7.10 |||hydantoin racemase family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 238
Score = 27.5 bits (58), Expect = 2.1
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -2
Query: 663 ET*RKNGAGIVQASALTATAGGIKVEI 583
ET RK GI+QAS LTA + G KV +
Sbjct: 89 ETYRKPCTGIMQASILTALSLGRKVSV 115
>SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 258
Score = 25.8 bits (54), Expect = 6.3
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +1
Query: 511 PDDANIVEVLHTTAGLIGYDYPLGDLDFY-PSGG 609
P D +I VLH TA +I + +DF+ P GG
Sbjct: 178 PLDFSITIVLHLTAVVISIIFSFAYMDFFLPRGG 211
>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
Ste6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 25.8 bits (54), Expect = 6.3
Identities = 17/72 (23%), Positives = 31/72 (43%)
Frame = +1
Query: 28 SFNLGERDVVFHLFHRGSPQVSEPLLLSVNSIMTSSFSLARRTIFTIHNHGETVAGNFNA 207
S N G+ + H F+R S + + LL+ S + ++ F + F
Sbjct: 545 SLNFGKISFISHEFYRVSKRFLDILLIWFESYLVEELDNSKSIFFLF-----KIYKVFEV 599
Query: 208 FVIPAHLAAEDV 243
FV+P +AE++
Sbjct: 600 FVVPHFASAEEL 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,777,915
Number of Sequences: 5004
Number of extensions: 52910
Number of successful extensions: 128
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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