Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0049
(827 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces pomb... 87 3e-18
SPBP35G2.07 |ilv1||acetolactate synthase catalytic subunit|Schiz... 41 2e-04
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 40 6e-04
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 30 0.35
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 29 0.61
SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|S... 27 2.5
SPAC1002.04c |taf11||transcription factor TFIID complex subunit ... 27 4.3
SPAC17A2.08c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 5.7
SPAC869.10c |||proline specific permease |Schizosaccharomyces po... 26 5.7
>SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 574
Score = 87.0 bits (206), Expect = 3e-18
Identities = 36/77 (46%), Positives = 56/77 (72%)
Frame = +3
Query: 9 ENTKLPFLPTPMGKGVVADESEYCVSTARTQALLKADVILLLGARMNWMLHFGQPPRYAP 188
E+T +PFLP+PMGKG++ + VS+AR+ AL ADV+LL GAR+NW+ +G PP+++P
Sbjct: 227 EHTGIPFLPSPMGKGLLPESHPLNVSSARSAALRNADVVLLAGARLNWIFQYGLPPKWSP 286
Query: 189 DVKIIQVEISPEEFHNS 239
+ K IQ++ + E N+
Sbjct: 287 NAKFIQIDTNAETLGNN 303
Score = 73.7 bits (173), Expect = 3e-14
Identities = 39/69 (56%), Positives = 46/69 (66%)
Frame = +1
Query: 511 GRGLLLNNHPRHRLDAGTFGTMGVGPGFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGME 690
GR LL HPR RLDAGT TMGVG G+AIA+A +++ VEGDSAFGFS ME
Sbjct: 401 GRQLLEVTHPRGRLDAGTMSTMGVGMGYAIASAF----AHSSDKIVVVEGDSAFGFSAME 456
Query: 691 IETMFRYKL 717
+ET R +L
Sbjct: 457 LETAIRNQL 465
Score = 27.1 bits (57), Expect = 3.2
Identities = 24/94 (25%), Positives = 44/94 (46%), Gaps = 5/94 (5%)
Frame = +2
Query: 242 EIRITVHSDIRPFTEALVKRLSERKFSLQPQNNSWWQGLKQKQKANTEFVEAQASSTAVP 421
++ + + +D+ + L K + K+S+ + + K + +E++ SS +
Sbjct: 306 DLDLAIWADVGLTIDCLFKLVQTWKYSVGISTPYLRTLNETRSKNEKKALESRKSSIPLQ 365
Query: 422 LNY--YTVFKTVQQGIPKDS---IIVSEGANTMD 508
+NY Y V + +Q K VSEGANTMD
Sbjct: 366 MNYALYVVNEELQSLSLKSKRNITWVSEGANTMD 399
>SPBP35G2.07 |ilv1||acetolactate synthase catalytic
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 669
Score = 40.7 bits (91), Expect = 2e-04
Identities = 22/61 (36%), Positives = 36/61 (59%)
Frame = +1
Query: 538 PRHRLDAGTFGTMGVGPGFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGMEIETMFRYKL 717
P + +G GTMG G AI A++ AP VI ++GD++F +GME+ T+ ++ +
Sbjct: 511 PSSLVTSGGLGTMGFGLPAAIGASV----AAPKDIVIDIDGDASFSMTGMELATVRQFDI 566
Query: 718 P 720
P
Sbjct: 567 P 567
>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 594
Score = 39.5 bits (88), Expect = 6e-04
Identities = 18/44 (40%), Positives = 27/44 (61%)
Frame = +1
Query: 589 GFAIAAAMWCRDYAPGKRVICVEGDSAFGFSGMEIETMFRYKLP 720
G+++ +AM AP +R I + GD +F +G EI M R+KLP
Sbjct: 422 GWSVPSAMGYAVAAPERRTIVMVGDGSFQLTGQEISQMIRHKLP 465
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 30.3 bits (65), Expect = 0.35
Identities = 23/79 (29%), Positives = 31/79 (39%)
Frame = +1
Query: 244 NQNYSTLRH*TFYRSSCKKVVRKEVLIATSKQQLVAGTETETKSKHRICRGSSKQHCSTT 423
+QN S RH S K + KE L ++ L T + K + +KQ S +
Sbjct: 820 SQNKSAARHEELKNLSTLKSLAKEFLSNYKEENLENSTLVQLKQLSKHLLSETKQDESFS 879
Query: 424 ELLHCFQNSSTRHSKRFNH 480
EL FQ S S H
Sbjct: 880 ELAKIFQEGSNITSHELLH 898
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 29.5 bits (63), Expect = 0.61
Identities = 21/93 (22%), Positives = 40/93 (43%), Gaps = 2/93 (2%)
Frame = +1
Query: 322 IATSKQQLVAGTETETKSKHRICRGSSKQHCSTTELLHC--FQNSSTRHSKRFNHCQRRS 495
++ S ++G+ T + + R R + H + +E H + NS ++K ++H Q
Sbjct: 269 VSASNSPALSGSSTPSNTSSRSNR---QNHGNFSEKRHYDRYGNSHPSYNK-YSHYQHGF 324
Query: 496 EYHGFGRGLLLNNHPRHRLDAGTFGTMGVGPGF 594
Y+ G + HPR R + G P +
Sbjct: 325 NYNNSGNNRNESGHPRFRNSRRNYNNQGAYPTY 357
>SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3'
ss-tail|Schizosaccharomyces pombe|chr 3|||Manual
Length = 957
Score = 27.5 bits (58), Expect = 2.5
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = +2
Query: 233 QQQEIRITVHSDIRPFTEALVKRLSER 313
QQ ++V SD+ PFT L+K++S+R
Sbjct: 229 QQPLSDVSVPSDVLPFTIKLLKKISQR 255
>SPAC1002.04c |taf11||transcription factor TFIID complex subunit
Taf11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 199
Score = 26.6 bits (56), Expect = 4.3
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +1
Query: 205 RSKYLLKNFTTARNQNYSTLRH*TFYRSSCKKVVRK 312
R+KYLL++F + Q Y R +++ KK+ +
Sbjct: 92 RTKYLLESFDEEQMQRYEVFRRANLNKTNVKKLANQ 127
>SPAC17A2.08c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 270
Score = 26.2 bits (55), Expect = 5.7
Identities = 13/42 (30%), Positives = 27/42 (64%), Gaps = 1/42 (2%)
Frame = +2
Query: 359 KQKQKANTEFVEAQA-SSTAVPLNYYTVFKTVQQGIPKDSII 481
K+++ ++T+ E Q SS +P+N + + QGIP++++I
Sbjct: 31 KKEEDSSTKESELQTRSSPPLPVNTSLEWNMLNQGIPEEAMI 72
>SPAC869.10c |||proline specific permease |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 552
Score = 26.2 bits (55), Expect = 5.7
Identities = 15/57 (26%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
Frame = -2
Query: 460 ALLNCFENSVVV-QWYCSAACLSLYKFGVCFLFLFQSLPPTVVL-RLQ*ELPFGQPF 296
A LN ++ VV W+C+ + +S + +C L + ++L L P+ PF
Sbjct: 396 AFLNASASAAVVFNWFCNLSTISGFLAWICVLVAYLQFRKAMILNNLWETRPYKTPF 452
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,536,542
Number of Sequences: 5004
Number of extensions: 72778
Number of successful extensions: 229
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 406444570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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