BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0044 (495 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 27 1.6 SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 25 6.3 SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 25 8.3 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 25 8.3 >SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 708 Score = 27.1 bits (57), Expect = 1.6 Identities = 15/36 (41%), Positives = 18/36 (50%) Frame = +2 Query: 35 IHHNGENLKGKRDRNRSPLRQKLVFYHQRLLNTKTE 142 IH L GK DRN P+ + V Y R+LN E Sbjct: 524 IHQTHLELHGK-DRNTPPMAKTFVTYLHRILNAIKE 558 >SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1010 Score = 25.0 bits (52), Expect = 6.3 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +1 Query: 364 VFYCSWSQSHYGINLTFFLLAGLV 435 +F+ + + HYG+N TF L LV Sbjct: 454 IFFLNLAFHHYGVNATFKALEQLV 477 >SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr 3|||Manual Length = 1647 Score = 24.6 bits (51), Expect = 8.3 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = -2 Query: 200 QCTRVAFAPNRYASSSVLCFQFWYLVV 120 QC + FA N+ SVL F + L+V Sbjct: 1526 QCLLITFAQNKLTVHSVLTFDYIKLMV 1552 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 24.6 bits (51), Expect = 8.3 Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 2/63 (3%) Frame = +2 Query: 29 AQIHHNGENLKGKRDRNRSPLRQKLVFYHQRLLNT--KTENTVLKKTRIDLVQRLHAYIA 202 AQ++ E+L K N+ QK V Q+ N KTE L+K+ + +QR + Sbjct: 670 AQVNLGAEDLIAKL--NKEVEDQKDVILSQKRTNETLKTEIDALQKSHVTQIQRSEVELR 727 Query: 203 KTY 211 + Y Sbjct: 728 ELY 730 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,128,913 Number of Sequences: 5004 Number of extensions: 43752 Number of successful extensions: 115 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 115 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 194131776 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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