BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0041 (778 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g17670.1 68418.m02071 expressed protein 30 1.5 At5g29090.1 68418.m03619 hypothetical protein 30 2.0 At5g38210.1 68418.m04606 serine/threonine protein kinase family ... 29 4.5 At2g03190.1 68415.m00272 E3 ubiquitin ligase SCF complex subunit... 28 6.0 At2g24650.1 68415.m02944 transcriptional factor B3 family protei... 28 7.9 >At5g17670.1 68418.m02071 expressed protein Length = 309 Score = 30.3 bits (65), Expect = 1.5 Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 4/82 (4%) Frame = +2 Query: 20 AAEHSGKMVMHDPFAMRPFXGYNFGDYLKHWPSMPQPG--RNMPKIFHVNWFRKDEQ--G 187 ++ S V + P + P G N GDY K ++ + G + + ++WFR Sbjct: 22 SSSSSAGNVKYRPAVILPGLGNNTGDYKKLEVTLGEYGVPAVVAAVSRLDWFRNAAGLVD 81 Query: 188 KFLWPGFGENSRVLDWILRRCD 253 W G VLDW L R D Sbjct: 82 PAYWRGTLRPRPVLDWYLNRID 103 >At5g29090.1 68418.m03619 hypothetical protein Length = 195 Score = 29.9 bits (64), Expect = 2.0 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = +2 Query: 197 WPGFGENSRVLDWILRRCDG 256 W G+ N RVLD + RCDG Sbjct: 54 WSGWASNVRVLDGVAGRCDG 73 >At5g38210.1 68418.m04606 serine/threonine protein kinase family protein contains protein kinase domain, Pfam:PF00069; contains serine/threonine protein kinase domain, INTERPRO:IPR002290 Length = 686 Score = 28.7 bits (61), Expect = 4.5 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = +1 Query: 277 PLGYIPRAGALNTENLSAV 333 P+GY P GALN NL +V Sbjct: 179 PIGYAPEEGALNVTNLESV 197 >At2g03190.1 68415.m00272 E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 (At16), putative E3 ubiquitin ligase; similar to Skp1 homolog Skp1a GI:3068807 from [Arabidopsis thaliana] Length = 170 Score = 28.3 bits (60), Expect = 6.0 Identities = 14/40 (35%), Positives = 24/40 (60%) Frame = +1 Query: 280 LGYIPRAGALNTENLSAVDMNELFSIPKDFWLQESDAIEK 399 LG + A + +++S ++ ELF+I D+ +E DAI K Sbjct: 121 LGLTCQTVADHMKDMSPEEVRELFNIENDYTPEEEDAIRK 160 >At2g24650.1 68415.m02944 transcriptional factor B3 family protein low similarity to reproductive meristem gene 1 from [Brassica oleracea var. botrytis] GI:3170424, [Arabidopsis thaliana] GI:13604227; contains Pfam profile PF02362: B3 DNA binding domain Length = 1440 Score = 27.9 bits (59), Expect = 7.9 Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 1/50 (2%) Frame = +2 Query: 581 FVMKTERSTLLFDTDNAPHTWVTGISVNKREG-LMFVSKIGRLCSLNLKI 727 FV STL +DT P ++ V+KR G ++ +++ G+ +L+LK+ Sbjct: 1187 FVANVAPSTLRYDTLYLPKRFMRENGVDKRRGEMILMNEKGKSWTLDLKL 1236 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,971,916 Number of Sequences: 28952 Number of extensions: 364391 Number of successful extensions: 1095 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 1061 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1095 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1736283200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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