Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0040
(740 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 124 6e-29
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 124 6e-29
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 31 0.80
At2g47550.1 68415.m05934 pectinesterase family protein contains ... 30 1.4
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 3.2
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 3.2
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein 29 4.3
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 28 5.7
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 27 9.9
At3g60160.1 68416.m06717 ABC transporter family protein similar ... 27 9.9
At1g18670.1 68414.m02330 protein kinase family protein contains ... 27 9.9
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 124 bits (299), Expect = 6e-29
Identities = 53/59 (89%), Positives = 56/59 (94%)
Frame = +3
Query: 3 VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 179
VSK+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 60 VSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 118
Score = 91.9 bits (218), Expect = 4e-19
Identities = 40/87 (45%), Positives = 59/87 (67%)
Frame = +1
Query: 478 QGSGSDSRFPHIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSK 657
+G+ F ++PGVE APGGHLGRFVIWT+SAF +L+ ++GS++ PS+
Sbjct: 220 EGAKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSE 279
Query: 658 QKKNFNLPQPKMANTDLTRLLKSDEIR 738
+KK + LP+ KM N DL R++ SDE++
Sbjct: 280 KKKGYVLPRAKMVNADLARIINSDEVQ 306
Score = 89.4 bits (212), Expect = 2e-18
Identities = 42/88 (47%), Positives = 58/88 (65%), Gaps = 1/88 (1%)
Frame = +2
Query: 257 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 436
ARGH IE +PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRN
Sbjct: 145 ARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRN 204
Query: 437 RRRIQRKGPLIIFNKDQG-LTRAFRTSP 517
RR I RKGPL++F + + +AFR P
Sbjct: 205 RRYISRKGPLVVFGTEGAKIVKAFRNLP 232
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 124 bits (299), Expect = 6e-29
Identities = 53/59 (89%), Positives = 56/59 (94%)
Frame = +3
Query: 3 VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 179
VSK+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 59 VSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 117
Score = 93.5 bits (222), Expect = 1e-19
Identities = 42/87 (48%), Positives = 59/87 (67%)
Frame = +1
Query: 478 QGSGSDSRFPHIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSK 657
+GS F ++PGVE APGGHLGRFVIWT+SAF +L+ ++GS++ PS+
Sbjct: 219 EGSKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSE 278
Query: 658 QKKNFNLPQPKMANTDLTRLLKSDEIR 738
+KK + LP+ KM N DL R++ SDEI+
Sbjct: 279 KKKGYVLPRAKMVNADLARIINSDEIQ 305
Score = 88.2 bits (209), Expect = 5e-18
Identities = 41/88 (46%), Positives = 58/88 (65%), Gaps = 1/88 (1%)
Frame = +2
Query: 257 ARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRN 436
ARGH IE +PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRN
Sbjct: 144 ARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRN 203
Query: 437 RRRIQRKGPLIIFNKD-QGLTRAFRTSP 517
RR I RKGPL+++ + + +AFR P
Sbjct: 204 RRYISRKGPLVVYGTEGSKIVKAFRNLP 231
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 31.1 bits (67), Expect = 0.80
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -3
Query: 138 HHDTCYRRHPDRTYGYHHHGHAEF 67
HH Y H YGY +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At2g47550.1 68415.m05934 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 560
Score = 30.3 bits (65), Expect = 1.4
Identities = 24/80 (30%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = -2
Query: 502 SASQTLILVED--YEGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGL 329
+A T+ +ED + SLT+D + T STKTL L +D+ L A ++
Sbjct: 92 AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQT--C 149
Query: 328 VDLLDFVGYNQGKLGNLFNN 269
++ L G G+LFN+
Sbjct: 150 LEGLKSTASENGLSGDLFND 169
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 134 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 232
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 134 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 232
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
Length = 320
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = -2
Query: 466 EGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKL 287
E SL +D A F ++ +K + L+ KD+R ++ ++ D++ G + K+
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301
Query: 286 GNL 278
G +
Sbjct: 302 GEI 304
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 28.3 bits (60), Expect = 5.7
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -3
Query: 339 CLVLLISWTLSATTKGSSGIFS 274
CLVLL+S +A TK SGIF+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFT 30
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 27.5 bits (58), Expect = 9.9
Identities = 15/58 (25%), Positives = 25/58 (43%), Gaps = 3/58 (5%)
Frame = +3
Query: 3 VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAPTKPW 167
+ + G + +AE + + + ++P G G G+ G FGN GG F W
Sbjct: 81 IRRRMGAKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGDISKW 138
>At3g60160.1 68416.m06717 ABC transporter family protein similar to
ATP-binding cassette transporter MRP8 GI:18031899 from
[Arabidopsis thaliana]
Length = 1490
Score = 27.5 bits (58), Expect = 9.9
Identities = 21/62 (33%), Positives = 31/62 (50%)
Frame = +2
Query: 359 RLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQGLTRAFRTSPVWSS*ML 538
R+KA S+ILK K +L+A + N+ + RK K L +AF T +W + L
Sbjct: 491 RMKATSEILKNMKILKLQAWDNQFLNKVKTLRKKEYDCLWKSLRL-QAFTTFILWGAPSL 549
Query: 539 TS 544
S
Sbjct: 550 IS 551
>At1g18670.1 68414.m02330 protein kinase family protein contains
Protein kinases ATP-binding region signature,
PROSITE:PS00107 and Serine/Threonine protein kinases
active-site signature, PROSITE:PS00108
Length = 662
Score = 27.5 bits (58), Expect = 9.9
Identities = 18/56 (32%), Positives = 26/56 (46%)
Frame = +1
Query: 244 QRSLG*RTHY*KDSRASLGCSRQSPGDQQDQTGCHLPEAPQGMV*YP*GVQVSASS 411
Q+ G H DS ++L Q P + + H+ A QG V + +QVS SS
Sbjct: 489 QKRNGHSVHNSIDSDSTLFEKMQKPSNHEKDEASHVKNASQGDVPFSGPLQVSVSS 544
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,168,333
Number of Sequences: 28952
Number of extensions: 336378
Number of successful extensions: 1181
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1094
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1173
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -