BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0039 (639 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 73 4e-14 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 50 2e-07 SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p... 28 0.99 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 3.0 SPCC794.06 |||TDT malic acid transporter|Schizosaccharomyces pom... 26 5.3 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 5.3 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 5.3 SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 7.0 >SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 72.9 bits (171), Expect = 4e-14 Identities = 35/85 (41%), Positives = 55/85 (64%), Gaps = 2/85 (2%) Frame = +1 Query: 16 ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVE 189 +++ + P VLF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE Sbjct: 21 LNIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVE 80 Query: 190 AELNYAMDVYELNRRVNSSESMLVG 264 E+ Y +Y L+ + N E ++VG Sbjct: 81 VEMEYHRAMYHLHLKANPRE-VVVG 104 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 50.4 bits (115), Expect = 2e-07 Identities = 26/83 (31%), Positives = 45/83 (54%), Gaps = 4/83 (4%) Frame = +1 Query: 22 VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA-- 192 V VHP+VL VD+Y R RV+G LLG ++ VV V N + +P +E Sbjct: 17 VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWF 76 Query: 193 -ELNYAMDVYELNRRVNSSESML 258 + N+ + E+ +++N++E ++ Sbjct: 77 LDHNFMESMNEMFKKINANEKLV 99 >SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 28.3 bits (60), Expect = 0.99 Identities = 17/63 (26%), Positives = 32/63 (50%), Gaps = 5/63 (7%) Frame = +1 Query: 7 ELNISVKVHPVVLFQIVDAYERRNADSHRVI-----GTLLGTSDKGVVEVTNCFCVPHKE 171 ELNI ++ +V+ ++++ ++RN D H I + TSD ++ + N F + Sbjct: 252 ELNIIIQEVDIVIGELIEGLKKRNIDKHVNIIFLSDHGMAPTSDNRLIWLDNMFNLSAVA 311 Query: 172 HAD 180 H D Sbjct: 312 HRD 314 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 3.0 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +1 Query: 64 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 189 Y+ R DSHR I L GT + + + + +K H ++E Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524 >SPCC794.06 |||TDT malic acid transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 431 Score = 25.8 bits (54), Expect = 5.3 Identities = 18/81 (22%), Positives = 38/81 (46%) Frame = +1 Query: 190 AELNYAMDVYELNRRVNSSESMLVGGRLAMK*PTTPLLYTSIIPVNAVSLSMLLWTLRWL 369 A + MDV EL R ++S +VG + T + ++ + AV + LL+ + Sbjct: 326 ANVGLVMDVQELGRAIDSKAVCIVGQVCGVT-ITIVWIILILLTLRAVYVQELLYPGKDE 384 Query: 370 EVEWVYVHMFVYHWECQMESK 432 +++ + ++ Y+ + E K Sbjct: 385 DIDTILPNVLEYYRHLEEEEK 405 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.8 bits (54), Expect = 5.3 Identities = 13/44 (29%), Positives = 24/44 (54%) Frame = -2 Query: 446 SEHATLLSIWHSQWYTNICT*THSTSSQRSVQSNMDRLTAFTGI 315 S A +L + W+ N+ T S+RS+ +N D +T+F+ + Sbjct: 410 SAKARILDSFCIDWHLNV-----QTMSERSILTNRDFITSFSAV 448 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 5.3 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +3 Query: 75 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 218 + R S SY I ++ SGG + + L A RTC S + T L + CL Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247 >SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 25.4 bits (53), Expect = 7.0 Identities = 6/11 (54%), Positives = 10/11 (90%) Frame = +2 Query: 437 HVHSCRCHSHM 469 H+H CRCH+++ Sbjct: 69 HIHCCRCHTYI 79 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,551,889 Number of Sequences: 5004 Number of extensions: 55630 Number of successful extensions: 156 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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