Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0035
(712 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 55 1e-08
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 52 1e-07
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 51 2e-07
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 46 4e-06
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 41 2e-04
SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 36 0.004
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 35 0.013
SPBC18E5.07 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 29 0.87
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 26 6.1
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 26 6.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.1
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 25 8.1
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 54.8 bits (126), Expect = 1e-08
Identities = 28/64 (43%), Positives = 42/64 (65%), Gaps = 3/64 (4%)
Frame = +3
Query: 525 GRAVKVPGFEKGN---FVGPTIITDVTPSMECYKEEIFGPVLVCLFVETLDEAIQMINSN 695
G +++ G GN FV PTI+++VT M KEEIFGPVL + +T++EAI+ N++
Sbjct: 366 GAKLEIGGKRHGNLGYFVEPTILSNVTEDMAVGKEEIFGPVLAVIKFKTIEEAIRRGNNS 425
Query: 696 PYGM 707
YG+
Sbjct: 426 TYGL 429
Score = 43.2 bits (97), Expect = 4e-05
Identities = 22/80 (27%), Positives = 41/80 (51%), Gaps = 2/80 (2%)
Frame = +2
Query: 17 PGATLMMMELLQEAGAPPGVVNIIHGT-HGAVNFICDQPEIKAVSFVGGDAAGKHIYTRA 193
P + L + + + E G PPGV+N++ G N I +I V+F G G+ + A
Sbjct: 200 PLSALCLTKFVPECGFPPGVINVLSGDGRRCGNAISSHMDIDKVAFTGSTGVGRMVMRAA 259
Query: 194 SAAG-KRVQSNMGAKNHGVI 250
+++ K+V +G K+ ++
Sbjct: 260 ASSNLKKVTLELGGKSPNIV 279
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +1
Query: 364 EWIPDLVKREQALKVNAGHVPGTDVGPVISVAAKDRILRLVESGVKEG 507
E+I +V + + LKV T G +S +RI+ +ESG+ G
Sbjct: 319 EFIKRMVAKAKTLKVGDPFAEDTFQGAQVSKQQYERIVSYIESGIAHG 366
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 51.6 bits (118), Expect = 1e-07
Identities = 23/52 (44%), Positives = 33/52 (63%)
Frame = +3
Query: 552 EKGNFVGPTIITDVTPSMECYKEEIFGPVLVCLFVETLDEAIQMINSNPYGM 707
++G F+ PTI D + +M KEEIFGPV+ +T DEAI+ N+ YG+
Sbjct: 373 KQGYFISPTIFADCSENMTIVKEEIFGPVVAISKFKTEDEAIEKANNTTYGL 424
Score = 39.9 bits (89), Expect = 4e-04
Identities = 25/75 (33%), Positives = 37/75 (49%), Gaps = 1/75 (1%)
Frame = +2
Query: 17 PGATLMMMELLQEAGAPPGVVNIIHGTHG-AVNFICDQPEIKAVSFVGGDAAGKHIYTRA 193
P + L L++EAG P GVVNII G A +++ P I ++F G G + A
Sbjct: 194 PLSLLYFATLVEEAGFPKGVVNIISGLGTVAGSYMAKHPGIDKIAFTGSTKVGVIVQQLA 253
Query: 194 SAAGKRVQSNMGAKN 238
++ K V G K+
Sbjct: 254 ASNLKAVTLECGGKS 268
Score = 27.1 bits (57), Expect = 2.6
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +1
Query: 430 TDVGPVISVAAKDRILRLVESGVKEG 507
T VGPV++ +RI +E G KEG
Sbjct: 335 TVVGPVVNKTQYNRIKNYIEQGKKEG 360
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 50.8 bits (116), Expect = 2e-07
Identities = 21/52 (40%), Positives = 34/52 (65%)
Frame = +3
Query: 552 EKGNFVGPTIITDVTPSMECYKEEIFGPVLVCLFVETLDEAIQMINSNPYGM 707
+KG F+ PT+ T+V + ++EEIFGPVL T +EA+++ N + YG+
Sbjct: 380 QKGYFIPPTVFTNVQTHNKIWREEIFGPVLAVKTFHTNEEALELANDSEYGL 431
Score = 33.9 bits (74), Expect = 0.023
Identities = 24/80 (30%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
Frame = +2
Query: 17 PGATLMMMELLQEAGAPPGVVNII--HGTHGAVNFICDQPEIKAVSFVGGDAAGKHIYTR 190
P + L + +EAG P GV+N+I G C P+I ++F G A GK I
Sbjct: 202 PWSCLEFALICKEAGLPDGVLNVIIGSGKESGAALSC-HPKIAYLAFTGSLATGKKIMHA 260
Query: 191 ASAAGKRVQSNMGAKNHGVI 250
A+ + +G K+ +I
Sbjct: 261 AAENIVPLTLELGGKSPLII 280
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 46.4 bits (105), Expect = 4e-06
Identities = 21/70 (30%), Positives = 38/70 (54%)
Frame = +3
Query: 498 KGRRKLALDGRAVKVPGFEKGNFVGPTIITDVTPSMECYKEEIFGPVLVCLFVETLDEAI 677
KG R L G + P + KGN+ PT++ D T M+ +EE F P+ + ++ + A+
Sbjct: 334 KGAR-LVYGGHRFQHPKYPKGNYFLPTLLVDATNEMKIAQEECFAPIALVFRAKSPEHAL 392
Query: 678 QMINSNPYGM 707
++ N +G+
Sbjct: 393 EIANGTEFGL 402
Score = 25.8 bits (54), Expect = 6.1
Identities = 16/69 (23%), Positives = 28/69 (40%)
Frame = +2
Query: 32 MMMELLQEAGAPPGVVNIIHGTHGAVNFICDQPEIKAVSFVGGDAAGKHIYTRASAAGKR 211
M+ LLQ G P +V I + + IK ++F+G K + A+
Sbjct: 176 MVRSLLQSMGHSPELVQCITCLPDVADHLTSHSGIKHITFIGSQPIAKLVAASAAKQLTP 235
Query: 212 VQSNMGAKN 238
+ +G K+
Sbjct: 236 LCLELGGKD 244
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 41.1 bits (92), Expect = 2e-04
Identities = 22/73 (30%), Positives = 39/73 (53%), Gaps = 2/73 (2%)
Frame = +2
Query: 17 PGATLMMMELLQEAGAPPGVVNIIHGTHGAVN--FICDQPEIKAVSFVGGDAAGKHIYTR 190
P L ++ L QEAG P GV+N+I + + + + P ++ VSF G GK + +
Sbjct: 194 PYVCLGLVRLAQEAGFPDGVLNVITSSDASAHGKELTTNPIVRKVSFTGSTNVGKILMGQ 253
Query: 191 ASAAGKRVQSNMG 229
+++ K+V +G
Sbjct: 254 SASTIKKVSMELG 266
Score = 36.7 bits (81), Expect = 0.003
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = +3
Query: 555 KGNFVGPTIITDVTPSMECYKEEIFGPVLVCLFVETLDEAIQMINSNPYGM 707
KG F PT+++ VT M EE FGP+ + +E I+ N + G+
Sbjct: 374 KGYFFEPTVLSGVTQDMLVASEETFGPLASVFKFDDTEEVIEWANDSDVGL 424
>SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate
dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 36.3 bits (80), Expect = 0.004
Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 3/54 (5%)
Frame = +3
Query: 555 KGNFVGPTIITDVTPSMECYKEEIFGPVLVCLFVE--TLDEAIQMIN-SNPYGM 707
+G FV PT++ P + + E+FGPVL E LD +I+ + PYG+
Sbjct: 410 EGFFVEPTVLLSKNPKHDIFVNELFGPVLSVYVYEDDNLDAVCDLIDTTTPYGL 463
Score = 31.1 bits (67), Expect = 0.16
Identities = 14/55 (25%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Frame = +2
Query: 23 ATLMMMELLQEAGAPPGVVNIIHGTHGAVNFIC-DQPEIKAVSFVGGDAAGKHIY 184
++ ++ ++ +EAG P G + I G V+ +C + PE + F G A + ++
Sbjct: 223 SSYIVYQIFREAGLPAGALQFIPGDAVEVSKVCFNHPEFAGLHFTGSTAVFRSLW 277
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 34.7 bits (76), Expect = 0.013
Identities = 16/45 (35%), Positives = 23/45 (51%)
Frame = +3
Query: 573 PTIITDVTPSMECYKEEIFGPVLVCLFVETLDEAIQMINSNPYGM 707
PT+I + M +EE FGPV +T DE + N +P G+
Sbjct: 385 PTVIINAKQGMLISEEETFGPVGALFKFDTEDEVVAWANDSPVGL 429
>SPBC18E5.07 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 615
Score = 28.7 bits (61), Expect = 0.87
Identities = 24/81 (29%), Positives = 34/81 (41%)
Frame = +1
Query: 346 FVGSAKEWIPDLVKREQALKVNAGHVPGTDVGPVISVAAKDRILRLVESGVKEGVNWLST 525
F GS ++ L R Q + VP P S E+GVKE +T
Sbjct: 479 FTGSRAGFVAALESRLQKGPLMRSFVPNKSKSP--SGTKSPASGETSEAGVKETET--TT 534
Query: 526 VARSKYPDSRKAISSGPLSSP 588
+ S PD+RK+ + GP+ P
Sbjct: 535 SSESALPDARKSRARGPVRRP 555
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 25.8 bits (54), Expect = 6.1
Identities = 28/93 (30%), Positives = 43/93 (46%), Gaps = 1/93 (1%)
Frame = -3
Query: 593 HVGDDSGPDEIAFLESGYFDRATVE-SQFTPSFTPDSTSLKILSLAATEMTGPTSVPGT* 417
H+ D+ PD I E D T E + P+ STS+ LSLA+T M+ S
Sbjct: 133 HLNPDTDPD-IPSDEDVLTDEDTEEVASIQPAL---STSVSSLSLASTAMSKSASASEFS 188
Query: 416 PALTFKACSRFTRSGIHSLALPTKTAVLSAMHR 318
+ KA + T S ++ +K A LS++ +
Sbjct: 189 GSSVTKASKKLTSS---PTSVASKKATLSSVSK 218
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 25.8 bits (54), Expect = 6.1
Identities = 12/40 (30%), Positives = 18/40 (45%)
Frame = -2
Query: 417 TRIDLQSLLTLHQVRDPLLSTADKDRSAERHAPLTGRSEG 298
TR + + LH+ + DK +S RHA G +G
Sbjct: 773 TRAERNEYILLHEFKKNGYIVPDKQQSIRRHAEAFGAEDG 812
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.1
Identities = 16/60 (26%), Positives = 30/60 (50%)
Frame = -3
Query: 518 SQFTPSFTPDSTSLKILSLAATEMTGPTSVPGT*PALTFKACSRFTRSGIHSLALPTKTA 339
S++TPS T S+ L S+++ + TSV + + + + T S SL+ T ++
Sbjct: 126 SEYTPSSTESSSLLDPSSVSSAILPSSTSVEVSISSSSLSSSDPLTSSTFSSLSSSTSSS 185
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 25.4 bits (53), Expect = 8.1
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = -3
Query: 626 YFLFVTLHARRHVGDDSGPDEI 561
Y F+ + RRH+GD PD +
Sbjct: 332 YVSFLPIMTRRHIGDYGVPDHV 353
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,655,063
Number of Sequences: 5004
Number of extensions: 48870
Number of successful extensions: 141
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -