Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0032
         (522 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyc...   105   4e-24
SPBC215.14c |vps20||vacuolar sorting protein Vps20|Schizosacchar...    27   1.7  
SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-...    25   5.2  
SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom...    25   6.8  
SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo...    25   9.0  
SPBC13E7.08c |||RNA polymerase II associated Paf1 complex |Schiz...    25   9.0  

>SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 126

 Score =  105 bits (252), Expect = 4e-24
 Identities = 49/89 (55%), Positives = 69/89 (77%), Gaps = 1/89 (1%)
 Frame = +1

Query: 34  MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 213
           MKF++ VTSSRRK RK HF APS +RRVLMS+PLSKELR+++ ++S+P+R+DD++ V+RG
Sbjct: 1   MKFSRDVTSSRRKQRKAHFGAPSSVRRVLMSAPLSKELREQYKIRSLPVRRDDQITVIRG 60

Query: 214 HYKGQQVGKVMQVYVKSLLYTL-RGFKEK 297
             KG++ GK+  VY K  L  + R  +EK
Sbjct: 61  SNKGRE-GKITSVYRKKFLLLIERVTREK 88



 Score = 60.9 bits (141), Expect = 1e-10
 Identities = 29/48 (60%), Positives = 38/48 (79%)
 Frame = +3

Query: 255 RKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRR 398
           RKKF++ IER+ REKANGA+A VGI  SK VI KL ++KDRK ++ R+
Sbjct: 74  RKKFLLLIERVTREKANGASAPVGIDASKVVITKLHLDKDRKDLIVRK 121


>SPBC215.14c |vps20||vacuolar sorting protein
           Vps20|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 226

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 4/48 (8%)
 Frame = +3

Query: 6   RGRFGEERQNEVQQAGDFLKKEKQEEAF----QCSFTYKASVDVLSPV 137
           +GR   ++++E+Q+  D L +E+++E      +  FT    VDVL  V
Sbjct: 140 QGRMSRDQEDEIQEELDSLIREQEDEKVKDLEKPGFTPSTGVDVLPSV 187


>SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase
           [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
 Frame = +3

Query: 321 VGIHPSKCV-IVKLKMNKDRKAILDRRAKGRLAALGKDKGKY 443
           +GI P K    +K  + KDR A ++RR +G    +G D+  Y
Sbjct: 256 IGIDPEKFSDALKSDVVKDRIASIERRLQGVKVIVGVDRLDY 297


>SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 986

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 11/27 (40%), Positives = 13/27 (48%)
 Frame = -2

Query: 443 VFTLVFAKCSQSALCSAIEDCFAVFIH 363
           +F LV    S   +C  IED F   IH
Sbjct: 588 LFNLVATNASDPYICGIIEDTFEDIIH 614


>SPBC4C3.07 |||translation initiation factor
           eIF3f|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 302

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 14/48 (29%), Positives = 21/48 (43%)
 Frame = +3

Query: 231 GWQSDAGVRKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKD 374
           GW + +     F   I+ +    A   TA +G +P  C  V L +N D
Sbjct: 104 GWYATSPDLDAFSALIQNLYASPAEPGTAPLGTYPHPC--VHLTVNTD 149


>SPBC13E7.08c |||RNA polymerase II associated Paf1 complex
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 429

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = +3

Query: 6   RGRFGEERQNEVQQAGDFLKKEKQEE 83
           RG   +  +NE    GDF+ +E++EE
Sbjct: 321 RGYNKDLEENEEDDLGDFIAEEEEEE 346


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,088,640
Number of Sequences: 5004
Number of extensions: 44160
Number of successful extensions: 140
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)