BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0030 (456 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g37720.1 68415.m04625 expressed protein 29 1.5 At4g36120.1 68417.m05141 expressed protein 28 2.6 At5g14570.2 68418.m01709 transporter, putative similar to trans-... 27 4.5 At5g14570.1 68418.m01708 transporter, putative similar to trans-... 27 4.5 At4g38640.1 68417.m05469 choline transporter-related contains we... 27 4.5 At4g13950.1 68417.m02158 rapid alkalinization factor (RALF) fami... 27 6.0 >At2g37720.1 68415.m04625 expressed protein Length = 482 Score = 29.1 bits (62), Expect = 1.5 Identities = 21/73 (28%), Positives = 35/73 (47%) Frame = +1 Query: 31 YAYRCTGVRIPQAGTNFSNEIRTQQMFTIDFHGEGITSCNXNQTSKIIICVITGGRTSWE 210 Y ++ G IP+ N N +R Q TI F G+ + + + ++C+ TGG+ S E Sbjct: 187 YRWQPEGCNIPEF--NRVNFLRRMQNKTIAFIGDSL----GREQFQSLMCMATGGKESPE 240 Query: 211 SARVGTTTPRLFP 249 VG+ + P Sbjct: 241 VQNVGSEYGLVIP 253 >At4g36120.1 68417.m05141 expressed protein Length = 981 Score = 28.3 bits (60), Expect = 2.6 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +3 Query: 225 YHHPPPISAVSTNAFRFEGWGSRC 296 +H+PP +++VS + F EG S C Sbjct: 432 HHYPPSVTSVSEDGFDEEGSSSEC 455 >At5g14570.2 68418.m01709 transporter, putative similar to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058, high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI:3608362; contains Pfam profile PF00083: Sugar (and other) transporter Length = 397 Score = 27.5 bits (58), Expect = 4.5 Identities = 13/46 (28%), Positives = 26/46 (56%) Frame = -1 Query: 177 NYNFAGLIXITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 40 NY A ++ + YS+ VE+ +++++ YF + G L + T A+ Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311 >At5g14570.1 68418.m01708 transporter, putative similar to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058, high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI:3608362; contains Pfam profile PF00083: Sugar (and other) transporter Length = 493 Score = 27.5 bits (58), Expect = 4.5 Identities = 13/46 (28%), Positives = 26/46 (56%) Frame = -1 Query: 177 NYNFAGLIXITRCYSFTVEVNREHLLSTYFIRKIGTRLRDSNTGAS 40 NY A ++ + YS+ VE+ +++++ YF + G L + T A+ Sbjct: 267 NYR-AWILALLYGYSYGVELTTDNVIAGYFYERFGVNLEAAGTIAA 311 >At4g38640.1 68417.m05469 choline transporter-related contains weak similarity to CD92 protein [Homo sapiens] gi|16945323|emb|CAC82175 Length = 556 Score = 27.5 bits (58), Expect = 4.5 Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 5/46 (10%) Frame = -1 Query: 186 DYAN---YNFAGLIXITRCYS--FTVEVNREHLLSTYFIRKIGTRL 64 DY N NFA + C S T E+ R +LLS F+ + TR+ Sbjct: 410 DYLNKFTINFAAITGEAYCTSAKMTYELLRRNLLSAVFVETVSTRI 455 >At4g13950.1 68417.m02158 rapid alkalinization factor (RALF) family protein similar to RALF precursor [Nicotiana tabacum] GI:16566316 Length = 113 Score = 27.1 bits (57), Expect = 6.0 Identities = 23/68 (33%), Positives = 30/68 (44%) Frame = -1 Query: 318 VLRSQYSYNGYPTLRTETH*CSQRK*AGGGGTYPCGLPRSPTTSDYANYNFAGLIXITRC 139 VL +Q Y GY TLR + C + G Y C RS + Y+ G ITRC Sbjct: 56 VLMAQKRYIGYETLRRDMVPCQK----PGASYYDC---RSGQANSYSR----GCDTITRC 104 Query: 138 YSFTVEVN 115 T ++N Sbjct: 105 ARDTNDIN 112 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,574,836 Number of Sequences: 28952 Number of extensions: 220523 Number of successful extensions: 442 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 438 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 442 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 752336160 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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