Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0027
(787 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) simila... 69 4e-12
At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) simila... 69 4e-12
At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 66 2e-11
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 30 2.0
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 29 3.5
At2g27270.1 68415.m03277 expressed protein 29 4.6
At5g43310.1 68418.m05293 COP1-interacting protein-related contai... 28 8.1
At3g22440.1 68416.m02836 hydroxyproline-rich glycoprotein family... 28 8.1
At3g05050.1 68416.m00548 protein kinase family protein contains ... 28 8.1
>At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 68.9 bits (161), Expect = 4e-12
Identities = 37/83 (44%), Positives = 52/83 (62%)
Frame = +2
Query: 266 PLKRRKSFYPTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILP 445
P+++ FYP ++ ++ R +K + +R ++ GTV I+LAGR GKRVV + L
Sbjct: 57 PVEKPPKFYPAED-VKKPLPNRRTAKPAK-LRASITPGTVLIILAGRFKGKRVVFLKQLA 114
Query: 446 SGLLLVTGPFAFNSCPLRRIPSA 514
SGLLLVTGPF N PLRR+ A
Sbjct: 115 SGLLLVTGPFKINGVPLRRVNQA 137
Score = 54.0 bits (124), Expect = 1e-07
Identities = 37/94 (39%), Positives = 48/94 (51%)
Frame = +1
Query: 472 FCIQFVPATPYS*RYVIGTSTRISLGNFKLPKHFNDDYFXXXXXXXXXXXXXXEGDDIFA 651
F I VP + YVIGTST++ + L K F+D YF EG+ A
Sbjct: 124 FKINGVPLRRVNQAYVIGTSTKVDISGVTLDK-FDDKYFGKVAEKKKKKT---EGEFFEA 179
Query: 652 TKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKK 753
K+EK + +K DQK VD A+IKAI A P+ K
Sbjct: 180 EKEEKKEIPQGKKDDQKAVDAALIKAIEAVPELK 213
>At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 68.9 bits (161), Expect = 4e-12
Identities = 37/83 (44%), Positives = 52/83 (62%)
Frame = +2
Query: 266 PLKRRKSFYPTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILP 445
P+++ FYP ++ ++ R +K + +R ++ GTV I+LAGR GKRVV + L
Sbjct: 57 PVEKPPKFYPAED-VKKPLPNRRTAKPTK-LRASITPGTVLIILAGRFKGKRVVFLKQLA 114
Query: 446 SGLLLVTGPFAFNSCPLRRIPSA 514
SGLLLVTGPF N PLRR+ A
Sbjct: 115 SGLLLVTGPFKINGVPLRRVNQA 137
Score = 54.0 bits (124), Expect = 1e-07
Identities = 37/94 (39%), Positives = 48/94 (51%)
Frame = +1
Query: 472 FCIQFVPATPYS*RYVIGTSTRISLGNFKLPKHFNDDYFXXXXXXXXXXXXXXEGDDIFA 651
F I VP + YVIGTST++ + L K F+D YF EG+ A
Sbjct: 124 FKINGVPLRRVNQAYVIGTSTKVDISGVTLDK-FDDKYFGKVAEKKKKKT---EGEFFEA 179
Query: 652 TKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKK 753
K+EK + +K DQK VD A+IKAI A P+ K
Sbjct: 180 EKEEKKEIPQVKKDDQKAVDAALIKAIEAVPELK 213
>At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar
to 60S ribosomal protein L6 GI:7208784 from [Cicer
arietinum]
Length = 233
Score = 66.1 bits (154), Expect = 2e-11
Identities = 36/83 (43%), Positives = 51/83 (61%)
Frame = +2
Query: 266 PLKRRKSFYPTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILP 445
P+++ FYP ++ ++ R K + ++ ++ GTV I+LAGR GKRVV + L
Sbjct: 57 PVEKPAKFYPAED-VKKPLVNRRKPKPTK-LKASITPGTVLIILAGRFKGKRVVFLKQLS 114
Query: 446 SGLLLVTGPFAFNSCPLRRIPSA 514
SGLLLVTGPF N PLRR+ A
Sbjct: 115 SGLLLVTGPFKINGVPLRRVNQA 137
Score = 56.8 bits (131), Expect = 2e-08
Identities = 38/95 (40%), Positives = 50/95 (52%)
Frame = +1
Query: 472 FCIQFVPATPYS*RYVIGTSTRISLGNFKLPKHFNDDYFXXXXXXXXXXXXXXEGDDIFA 651
F I VP + YVIGTST+I + K F+D YF EG+ A
Sbjct: 124 FKINGVPLRRVNQAYVIGTSTKIDISGVNTEK-FDDKYFGKVAEKKKKKT---EGEFFEA 179
Query: 652 TKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKV 756
K+EK +++K DQKTVD A+IK+I A P+ KV
Sbjct: 180 EKEEKKEIPQEKKEDQKTVDAALIKSIEAVPELKV 214
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 29.9 bits (64), Expect = 2.0
Identities = 12/31 (38%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = +2
Query: 266 PLKRRKSFYPTQEKIRASSGGRPFS-KHVRR 355
P++RR+S P +E++ S GGR S H+++
Sbjct: 525 PVRRRRSLTPDEERVSLSQGGRHTSPSHIKQ 555
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis
thaliana}
Length = 1473
Score = 29.1 bits (62), Expect = 3.5
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = +1
Query: 649 ATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKV 756
ATK K P+ RK ++TV + AIG P+KKV
Sbjct: 1354 ATKAAK-PPAAPRKRGKQTVASTEVLAIGVSPEKKV 1388
>At2g27270.1 68415.m03277 expressed protein
Length = 231
Score = 28.7 bits (61), Expect = 4.6
Identities = 22/76 (28%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
Frame = +2
Query: 293 PTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILPSGLLLVTGP 472
PT++K A G SKH R + + + C+ GK ++ G+ + +LL+
Sbjct: 21 PTKKKTEAKEEGAAASKHSRVVYSD---KSRCL----SKNGKTIIYFGVPAALILLIICV 73
Query: 473 FAFNSCPLR-RIPSAM 517
FAFN ++ R+P M
Sbjct: 74 FAFNYIAIQPRVPRFM 89
>At5g43310.1 68418.m05293 COP1-interacting protein-related contains
similarity to COP1-Interacting Protein 7 (CIP7)
[Arabidopsis thaliana] GI:3327868
Length = 1237
Score = 27.9 bits (59), Expect = 8.1
Identities = 21/72 (29%), Positives = 35/72 (48%), Gaps = 3/72 (4%)
Frame = -1
Query: 442 QNSNKYNPLACMSTSEENANSSYLQVGSDPAYMLAEWTA-TR*GTDFLLSG--VEGLPTF 272
+NS+ +P + ++ N N Y+Q G M A W + GT + G ++G+P +
Sbjct: 334 ENSDAKSP----TDNKRNGNQEYVQ-GQHRQPMYAPWPVHSPPGTFPVFQGYTMQGMPYY 388
Query: 271 EGYCSGPPFFSP 236
GY P+ SP
Sbjct: 389 PGYPGASPYPSP 400
>At3g22440.1 68416.m02836 hydroxyproline-rich glycoprotein family
protein identical to hydroxyproline-rich glycoprotein
[Arabidopsis thaliana] gi|9293881|dbj|BAB01784
Length = 532
Score = 27.9 bits (59), Expect = 8.1
Identities = 10/20 (50%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
Frame = -1
Query: 286 GLPTFEGYCSGP-PFFSPPI 230
G P + GYC+GP P +PP+
Sbjct: 495 GYPAYNGYCNGPVPAPAPPV 514
>At3g05050.1 68416.m00548 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 593
Score = 27.9 bits (59), Expect = 8.1
Identities = 22/67 (32%), Positives = 34/67 (50%)
Frame = +2
Query: 551 TSNCQNTSMMITSRRIRSASNVQSNAKRVMTSLPQKKRNTFHLSSAKPIRRQSMRL*SKP 730
T ++TS ++ + SASN + + + QKK N F ++ AK + + R SKP
Sbjct: 40 TETTESTSAVV----VASASNGEE-VRNHEDVVDQKKENGFVVTEAKERKSKGERKRSKP 94
Query: 731 LEPDPTR 751
PDP R
Sbjct: 95 --PDPRR 99
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,425,438
Number of Sequences: 28952
Number of extensions: 368310
Number of successful extensions: 1030
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 988
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1027
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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