Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV32544
(663 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 63 3e-11
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 55 1e-08
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 52 6e-08
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 48 1e-06
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 37 0.002
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 36 0.007
SPAC1783.02c |vps66||acyltransferase |Schizosaccharomyces pombe|... 28 1.0
SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyc... 28 1.4
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 27 1.8
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 25 7.4
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 25 7.4
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 9.7
SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 25 9.7
SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c... 25 9.7
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 63.3 bits (147), Expect = 3e-11
Identities = 40/169 (23%), Positives = 79/169 (46%), Gaps = 8/169 (4%)
Frame = +3
Query: 63 LTFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINSAYS 242
L+F ++ + + A A+++LG+ KGD + + +L T A L T+
Sbjct: 97 LSFNDIYDKALRYAGALRKLGLNKGDKFELYAPTSAFWLLTAEACLSQSMTIVTAYDTLG 156
Query: 243 KSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFFLFGISDDKDVTSFKDL--VT 416
+ +H+ + S ++ K LK++ ++ + ++D+ + +++
Sbjct: 157 EEGLLHSLRESGVRGMYTEGHLLKTLVNPLKEIESLEVIIYRNDAKEEDIKTIQEIRPNL 216
Query: 417 KYTHFNDFE------SNDFNGTDSTSIILYSSGTTGLPKGVKLTHRNLL 545
K F DFE D + I+Y+SG+TGLPKGV L+H+N++
Sbjct: 217 KLIKFADFEKMSPPVEPDPPSPEEICCIMYTSGSTGLPKGVILSHKNMV 265
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 54.8 bits (126), Expect = 1e-08
Identities = 45/169 (26%), Positives = 78/169 (46%), Gaps = 10/169 (5%)
Frame = +3
Query: 66 TFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINSAYSK 245
T+ ++ + +A + + GI +GDVV + + + + + VL GAT + I+ AY
Sbjct: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
Query: 246 SEFIHTTKISKPNYI-------FLSPSA--YKEFYVTLKQLNIVQKFFLFGISDDKDVTS 398
+ I ++KP + LSP+ Y E + LK K G V+
Sbjct: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
Query: 399 FKDLVTKYT-HFNDFESNDFNGTDSTSIILYSSGTTGLPKGVKLTHRNL 542
D + ++ H ++ G DST + ++SG+ G+PKGVK H +L
Sbjct: 434 GADDILQHVLHLKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSL 482
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 52.4 bits (120), Expect = 6e-08
Identities = 46/175 (26%), Positives = 74/175 (42%), Gaps = 5/175 (2%)
Frame = +3
Query: 33 ALINGASGEKLTFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGA 212
AL+ + +L+F E+ I+++ I LGI GD V I N E++ AV A
Sbjct: 18 ALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQRA 77
Query: 213 TVTFINSAYSKSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFFLFGISDDKDV 392
+NS Y +SEF K + + + + +K ++ +
Sbjct: 78 ICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVA---ANTPAVRAAKK---LSVAVAELA 131
Query: 393 TSFKDLVTKYTHFNDFESN-----DFNGTDSTSIILYSSGTTGLPKGVKLTHRNL 542
K + + HF + N D ++L++SGTTG PK V LTH+NL
Sbjct: 132 WCPKSRLVRIVHFEGAKINAPQPLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKNL 186
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 48.0 bits (109), Expect = 1e-06
Identities = 43/172 (25%), Positives = 79/172 (45%), Gaps = 2/172 (1%)
Frame = +3
Query: 60 KLTFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINSAY 239
+LT+ E+ ++ +A +K G G ++A+ + E + +A+L G ++ +
Sbjct: 2751 RLTYSELNERANKLAHNLKSYGFRVGSIIAVYFDKCIEAFISMLAILKAGCCFLALDVS- 2809
Query: 240 SKSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFFLFGISDDKDVTSFKDLVTK 419
+ +E I + +S E Y L LN + SD + ++ ++
Sbjct: 2810 APTERIRYIVTDSTAVLVMSTG---ELYTKL--LNASINVTILDASDPGNYSN--NIENP 2862
Query: 420 YTHFNDFESNDFNGTDSTSIILYSSGTTGLPKGVKLTHRNLL--VTGFQKSF 569
YT DFE ++ + +LY+SG+TG PKG LTH N++ + FQ F
Sbjct: 2863 YT--KDFEDSNL------AYVLYTSGSTGKPKGCCLTHHNVVQCMLAFQDQF 2906
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/24 (58%), Positives = 20/24 (83%)
Frame = +3
Query: 465 DSTSIILYSSGTTGLPKGVKLTHR 536
DS + +LY+SG+TG PKGV ++HR
Sbjct: 359 DSVAYVLYTSGSTGNPKGVAISHR 382
Score = 26.6 bits (56), Expect = 3.2
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 111 IKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGA 212
+ +GI K DVVA+ + +Y + + VL GA
Sbjct: 1797 LHSIGIKKNDVVAVFIDKSLDYYSLMLGVLRIGA 1830
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 37.1 bits (82), Expect = 0.002
Identities = 37/173 (21%), Positives = 73/173 (42%), Gaps = 17/173 (9%)
Frame = +3
Query: 54 GEKLTFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINS 233
G +T+ E+ + A A++ +G+G GD VAI E + +A++ GA + I +
Sbjct: 115 GRYITYRELLASVSQCAGALQSMGVGMGDRVAIYMPMIPETIIAMLAIVRLGAIHSVIFA 174
Query: 234 AYSKSEFIHTTKISKPNYIFLSPSAYK---------EFYVTLKQLNIVQKFFLF------ 368
+S S+ I + +++ L + ++K +F
Sbjct: 175 GFSAESVADRVNDSECKVIITADESHRGGKRIPLKGVVNKALTECPTIKKVLVFQRSAEP 234
Query: 369 --GISDDKDVTSFKDLVTKYTHFNDFESNDFNGTDSTSIILYSSGTTGLPKGV 521
+ + +DV + D++ K+ + + +LY+SG+TG PKGV
Sbjct: 235 TASMVEGRDVW-WHDIIPKFPRYC---PPAVVNPEHPLFLLYTSGSTGKPKGV 283
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 35.5 bits (78), Expect = 0.007
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = +3
Query: 465 DSTSIILYSSGTTGLPKGVKLTHRNLL 545
D +Y+SG+TG PKGV L HRN++
Sbjct: 245 DDICCYMYTSGSTGKPKGVVLLHRNII 271
>SPAC1783.02c |vps66||acyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 328
Score = 28.3 bits (60), Expect = 1.0
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -2
Query: 266 SCMYEFTFAICTVYESNRSSCTKHSYSCSQILCP 165
SC+Y TFA+C SN S + +Y S P
Sbjct: 153 SCLYNCTFAVCDSKTSNVSIISAQAYFWSCFFSP 186
>SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1088
Score = 27.9 bits (59), Expect = 1.4
Identities = 20/89 (22%), Positives = 34/89 (38%), Gaps = 2/89 (2%)
Frame = +3
Query: 201 CTGATVTFINSAYSKSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFF--LFGI 374
C + S + E I T + S YK+ LK+LN + F +
Sbjct: 132 CNSRIIALKQSLEASQECIGNTNSKELQQTLARSSQYKKVISVLKELNEANQLFDNFHTL 191
Query: 375 SDDKDVTSFKDLVTKYTHFNDFESNDFNG 461
D K DL+ + +++ +DF+G
Sbjct: 192 VDSKQYYHASDLIRRV--WDELSRSDFDG 218
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 27.5 bits (58), Expect = 1.8
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = -3
Query: 658 LFLLIFNKVKIIPTVLHHGAKVKTTKSF*QNDF*KPVTS-KLRCVNFTP 515
+FL+ +NK+K P ++ HG K T + K + S K NFTP
Sbjct: 246 MFLIAYNKLKSNPGMMTHGLKPDTLDGMSIDVIDKIIQSLKSEEFNFTP 294
>SPAC328.04 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 25.4 bits (53), Expect = 7.4
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = -3
Query: 193 AIVVVRYSVLFSLQIAT-TSPFPMPSCLIAEAIFTICCVISPNVNF 59
A+ SV FS+ ++ TS F S + A+FT+ +SP++ F
Sbjct: 511 AVATESRSVFFSISASSLTSKFLGESEKLVRALFTLAKKLSPSIIF 556
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.4 bits (53), Expect = 7.4
Identities = 11/31 (35%), Positives = 15/31 (48%)
Frame = -2
Query: 116 FNSRSYIYNLLCHFAKCQFLSASSIDQSHFC 24
F+ S + + HF QF S S I +H C
Sbjct: 264 FSGDSSLKSFSLHFFALQFFSTSLIQYTHIC 294
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.0 bits (52), Expect = 9.7
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +2
Query: 62 IDIWRNDTANCKYSF 106
+DIWRND N K+ +
Sbjct: 1334 LDIWRNDQPNSKFVY 1348
>SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 745
Score = 25.0 bits (52), Expect = 9.7
Identities = 25/87 (28%), Positives = 38/87 (43%)
Frame = +3
Query: 210 ATVTFINSAYSKSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFFLFGISDDKD 389
A+ +FI + + T K KP I S T K ++ +K F G+SD K
Sbjct: 524 ASKSFIGEVQDSLQALKT-KAHKPRSIGGDSSQTTLSETTKKYGSVAKKSFFQGVSDAK- 581
Query: 390 VTSFKDLVTKYTHFNDFESNDFNGTDS 470
SF + K T+ +D SN + +S
Sbjct: 582 --SFVKKI-KSTYIDDSSSNSPSDIES 605
>SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1315
Score = 25.0 bits (52), Expect = 9.7
Identities = 16/57 (28%), Positives = 23/57 (40%)
Frame = -2
Query: 233 TVYESNRSSCTKHSYSCSQILCPVFTANSYHIAFPNAKLFNSRSYIYNLLCHFAKCQ 63
TV R S + SY+ + VFT +A K N+ + NL H A +
Sbjct: 833 TVDIEERQSLIELSYTLFKKAAHVFTPEDLRLAVEEYKSLNAYTTAVNLALHVASAR 889
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,693,037
Number of Sequences: 5004
Number of extensions: 58117
Number of successful extensions: 185
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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