Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV32343
(605 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 330 7e-92
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 330 7e-92
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 330 7e-92
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 102 5e-23
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 1e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 53 3e-08
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 2.8
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 26 3.7
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 4.9
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 6.5
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 6.5
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 8.6
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 25 8.6
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 8.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 330 bits (812), Expect = 7e-92
Identities = 153/201 (76%), Positives = 172/201 (85%)
Frame = +2
Query: 2 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKN 181
KIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKN
Sbjct: 253 KIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKN 312
Query: 182 VSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAE 361
VSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 313 VSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Query: 362 IKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 541
+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 373 LIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432
Query: 542 VGVIKAVNFKEAGGGKVTKAA 604
VGVIKAV G KVTKAA
Sbjct: 433 VGVIKAVEKVAPGAAKVTKAA 453
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 330 bits (812), Expect = 7e-92
Identities = 153/201 (76%), Positives = 172/201 (85%)
Frame = +2
Query: 2 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKN 181
KIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKN
Sbjct: 253 KIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKN 312
Query: 182 VSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAE 361
VSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 313 VSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Query: 362 IKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 541
+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 373 LIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432
Query: 542 VGVIKAVNFKEAGGGKVTKAA 604
VGVIKAV G KVTKAA
Sbjct: 433 VGVIKAVEKVAPGAAKVTKAA 453
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 330 bits (812), Expect = 7e-92
Identities = 153/201 (76%), Positives = 172/201 (85%)
Frame = +2
Query: 2 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKN 181
KIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKN
Sbjct: 253 KIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKN 312
Query: 182 VSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAE 361
VSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 313 VSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Query: 362 IKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 541
+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 373 LIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432
Query: 542 VGVIKAVNFKEAGGGKVTKAA 604
VGVIKAV G KVTKAA
Sbjct: 433 VGVIKAVEKVAPGAAKVTKAA 453
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 102 bits (244), Expect = 5e-23
Identities = 60/190 (31%), Positives = 101/190 (53%), Gaps = 2/190 (1%)
Frame = +2
Query: 2 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVK 178
K +GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD V V+
Sbjct: 476 KYKDLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVR 535
Query: 179 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 358
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA
Sbjct: 536 GDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFA 593
Query: 359 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 538
++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TV
Sbjct: 594 KLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTV 652
Query: 539 AVG-VIKAVN 565
AVG V+K ++
Sbjct: 653 AVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 54.4 bits (125), Expect = 1e-08
Identities = 49/176 (27%), Positives = 79/176 (44%), Gaps = 1/176 (0%)
Frame = +2
Query: 32 GRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRG 208
GRVE G ++ ++ + VK+V + + AV GD V + ++ V +LR G
Sbjct: 423 GRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPG 482
Query: 209 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 388
+ + +N P + F A++ + G I +G T VL H+ + K+
Sbjct: 483 DILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVN 536
Query: 389 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 556
K + + S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 537 NKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 53.2 bits (122), Expect = 3e-08
Identities = 47/185 (25%), Positives = 80/185 (43%), Gaps = 2/185 (1%)
Frame = +2
Query: 5 IGGIGTVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVK 178
I G GTV GRVE G LK G + V +++ T V +EM + L AV GDN G ++
Sbjct: 263 ISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLR 322
Query: 179 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 358
++ ++L+RG + P F A +L + T +D + + + +
Sbjct: 323 SIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQLYSRTS 378
Query: 359 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 538
++ ++ T + K + GD + P+ +E Q RF VR+ TV
Sbjct: 379 DVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVREGGSTV 429
Query: 539 AVGVI 553
++
Sbjct: 430 GTALV 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 199 QFLDGHVLYVETYIVSRYSFLESFV 125
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 26.2 bits (55), Expect = 3.7
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = -3
Query: 105 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIP 10
+SVV+ + +T V + + VST TGTV +P
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVP 117
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 4.9
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -2
Query: 223 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 107
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 6.5
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 54 NTPVSTLPTGTVPIPPIL 1
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 6.5
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 441 MAASPDLMDFGLTSVDLPVRRSTFS 367
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.0 bits (52), Expect = 8.6
Identities = 23/82 (28%), Positives = 41/82 (50%), Gaps = 2/82 (2%)
Frame = -3
Query: 597 LVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAASPD 424
+VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++S
Sbjct: 186 IVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSSSSV 244
Query: 423 LMDFGLTSVDLPVRRSTFSLIS 358
L +TS PV S+ SL S
Sbjct: 245 LPTSIITSTSTPVTVSSSSLSS 266
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 25.0 bits (52), Expect = 8.6
Identities = 13/38 (34%), Positives = 16/38 (42%)
Frame = -3
Query: 141 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTLPT 28
SW SW +ST L +V + G T ST T
Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRT 134
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 25.0 bits (52), Expect = 8.6
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -3
Query: 594 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 472
+ L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,758,352
Number of Sequences: 5004
Number of extensions: 60567
Number of successful extensions: 194
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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