Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV32253
(632 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17G8.08c |||human TMEM165 homolog|Schizosaccharomyces pombe|... 73 3e-14
SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|c... 62 9e-11
SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 3.0
SPBC409.19c |||metaxin|Schizosaccharomyces pombe|chr 2|||Manual 26 3.9
SPBPB2B2.05 |||GMP synthase [glutamine-hydrolyzing] |Schizosacch... 26 5.2
SPCC320.05 |||sulphate transporter |Schizosaccharomyces pombe|ch... 25 6.9
SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr ... 25 6.9
>SPAC17G8.08c |||human TMEM165 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 287
Score = 73.3 bits (172), Expect = 3e-14
Identities = 36/84 (42%), Positives = 54/84 (64%), Gaps = 1/84 (1%)
Frame = +3
Query: 324 NFWQGFLASLSVVIVSELGDKTFFIAAIMAMKHPRIIVFAGAISALVFMTILSAAFGWIA 503
+F + + S+S++ E+GDKTF +AA++A ++ R+ VFAG+ SAL MT+L G A
Sbjct: 45 DFLRSLIFSISMIFGCEIGDKTFIVAALLAFENSRLTVFAGSYSALFIMTLLGVLLGHAA 104
Query: 504 TVI-PRVYTYYVSAALFAIFGIKM 572
++ PR T + LF IFGIKM
Sbjct: 105 PLLFPRKLTDILGGVLFVIFGIKM 128
Score = 32.3 bits (70), Expect = 0.060
Identities = 23/80 (28%), Positives = 35/80 (43%), Gaps = 1/80 (1%)
Frame = +3
Query: 339 FLASLSVVIVSELGDKTFFIAAIMAMKHPRIIVFAGAISALVFMTILSAAFG-WIATVIP 515
F+ + ++ VSE GD++ MA VF GA T L+ G +I+T I
Sbjct: 203 FIKAFALTFVSEWGDRSQIATIAMAASDNVYGVFMGANVGHACCTALAVISGKYISTKIK 262
Query: 516 RVYTYYVSAALFAIFGIKMF 575
++ LF FG+ F
Sbjct: 263 VHKVMFIGGILFIAFGLVYF 282
>SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 262
Score = 61.7 bits (143), Expect = 9e-11
Identities = 31/78 (39%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Frame = +3
Query: 348 SLSVVIVSELGDKTFFIAAIMAMKHPRIIVFAGAISALVFMTILSAAFGWIAT-VIPRVY 524
S+S++I ELGDK+F + A++A ++ R VF G+ AL FMT + G A + P+
Sbjct: 30 SISMIIGCELGDKSFIVTALLAYQYGRASVFFGSYLALFFMTSFAVLVGRAAPFLFPKSI 89
Query: 525 TYYVSAALFAIFGIKMFE 578
T+ + LF IFG+KM +
Sbjct: 90 THILGGTLFLIFGVKMLK 107
Score = 41.1 bits (92), Expect = 1e-04
Identities = 24/87 (27%), Positives = 45/87 (51%), Gaps = 2/87 (2%)
Frame = +3
Query: 327 FWQGFLASLSVVIVSELGDKTFFIAAIMAMKHPRIIVFAGAISALVFMTILSAAFG-WIA 503
F + F+ + +++ VSELGD++ +M+ K + VF G + T+++ G +I+
Sbjct: 175 FSRAFIKAFALIFVSELGDRSQIATIVMSAKEKVLDVFIGVNIGHMLCTMVAVIVGRYIS 234
Query: 504 TVIPRVYTYYVSAALFAIFGI-KMFEG 581
I + +F IFGI +F+G
Sbjct: 235 NKIEMYKVLFFGGIVFMIFGILYIFQG 261
>SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 409
Score = 26.6 bits (56), Expect = 3.0
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +3
Query: 294 TTTERTALQGNFWQGFLASLSVVIVSELGDKTFFIAAIMAMKHPRIIVFAG--AISALVF 467
T + R Q ++W FL+ LS+ +LG K F +I+++ VFAG +++ L
Sbjct: 257 TYSNRLHSQSHWWNMFLSQLSLDGTKDLGPK-FLEQSILSIPDIPEDVFAGHNSLNGLYA 315
Query: 468 MTILSA 485
+ SA
Sbjct: 316 LVFASA 321
>SPBC409.19c |||metaxin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 450
Score = 26.2 bits (55), Expect = 3.9
Identities = 14/39 (35%), Positives = 17/39 (43%)
Frame = -2
Query: 436 TMILGCFIAIIAAMKNVLSPSSETMTTDNEARNPCQKLP 320
TM GC A+I V E + T N +P KLP
Sbjct: 15 TMDPGCLAALIYCALAVPKDEIEILRTANSGMSPTHKLP 53
>SPBPB2B2.05 |||GMP synthase [glutamine-hydrolyzing]
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 237
Score = 25.8 bits (54), Expect = 5.2
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +2
Query: 449 YFGPCIHDDTIGCFRLDRHRDPKSVHVLRQRGLVRHIRHQNV*GT 583
YFG ++ + C D HR K H L + + + + +N+ G+
Sbjct: 93 YFGGSLYQNVSSCGFRDIHRPSKPRHYLAHKVMAKPGKLKNILGS 137
>SPCC320.05 |||sulphate transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 667
Score = 25.4 bits (53), Expect = 6.9
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 408 MAMKHPRIIVFAGAISALVFMTILSAAFGW 497
++ K+PRI+ A+ L+ + LS F W
Sbjct: 268 LSAKYPRIVSIPDAVIILLLGSFLSKKFDW 297
>SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 673
Score = 25.4 bits (53), Expect = 6.9
Identities = 20/65 (30%), Positives = 27/65 (41%), Gaps = 1/65 (1%)
Frame = +3
Query: 420 HPRIIVFAGAISAL-VFMTILSAAFGWIATVIPRVYTYYVSAALFAIFGIKMFEGRLENG 596
H +I F+ I AL V GW+ T + + T VSA +F KM L G
Sbjct: 397 HLSVIGFSLFIGALSVGFNYAGVTAGWLLTFLGIILTPEVSAVTLCLFWNKMTRFSLVVG 456
Query: 597 PQRGS 611
G+
Sbjct: 457 APFGT 461
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,521,598
Number of Sequences: 5004
Number of extensions: 46764
Number of successful extensions: 127
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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