Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV32148
(620 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 244 1e-65
SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p... 48 8e-07
SPBP23A10.14c |ell1||RNA polymerase II transcription elongation ... 29 0.54
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 29 0.54
SPAC1B1.03c |kap95||karyopherin Kap95|Schizosaccharomyces pombe|... 28 0.95
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 2.2
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 27 2.9
SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 26 3.8
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 26 3.8
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 25 6.7
SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha... 25 6.7
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 8.8
>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 619
Score = 244 bits (596), Expect = 1e-65
Identities = 112/204 (54%), Positives = 156/204 (76%)
Frame = +3
Query: 3 AVSPPGGDFSDPVTAATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYEKN 182
AVSPPGGDFSDPVT+ATLGIVQVFWGLDKKLAQRKHFPSIN +SYSKY+ AL +YE+
Sbjct: 417 AVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINTSLSYSKYINALQPWYEER 476
Query: 183 YPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSY 362
P F LR ++K+I+Q+E+ + EI+QLVGK++L+ETDK+TL++A ++K+DFLQQN YS Y
Sbjct: 477 VPGFNTLRDQIKQIIQQEDSMLEIIQLVGKSALSETDKVTLDIAGIIKNDFLQQNGYSDY 536
Query: 363 DRFCPFYKTVGMLKNIITFYDMSRHAVESTAQSDNKVTWNVIRDAMGNVLYQLSSMKFKD 542
DR CP YKT M++N+I +Y ++ AVE+ V W+ I+++ ++ Y+L+SMKF++
Sbjct: 537 DRCCPLYKTYHMMRNMIAYYTKAKSAVET-----GSVPWSKIKESTSDIFYELTSMKFEN 591
Query: 543 PVKDGEPKIKADFDQLLKDMSAAF 614
P +GE +I ++ L K + F
Sbjct: 592 P-NEGEKEIVEHYETLHKKIEDKF 614
>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 525
Score = 48.4 bits (110), Expect = 8e-07
Identities = 35/116 (30%), Positives = 61/116 (52%), Gaps = 2/116 (1%)
Frame = +3
Query: 3 AVSPPGGDFSDPVTAATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYM--RALDDFYE 176
AV P D +DP A T + L + +++ +P+++ L S S+ M R L +
Sbjct: 355 AVYVPADDLTDPAPATTFAHLDATTVLSRSISELGIYPAVDPLDSKSRMMDPRILGE--- 411
Query: 177 KNYPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQ 344
E L V+++LQE + L +I+ ++G L+E DK+T+E A+ ++ FL Q
Sbjct: 412 ----EHYNLAGSVQQMLQEYKSLQDIIAILGMDELSEADKLTVERARKVQ-RFLSQ 462
>SPBP23A10.14c |ell1||RNA polymerase II transcription elongation
factor SpELL|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 29.1 bits (62), Expect = 0.54
Identities = 20/73 (27%), Positives = 38/73 (52%)
Frame = +3
Query: 174 EKNYPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSY 353
+KN + + LRT+V ++L + + ++L KASL++ + EVA +N+
Sbjct: 189 DKNSAKGIDLRTRVIQLLAIAPETEDFLRLRTKASLSKLQALLPEVA--------WKNNM 240
Query: 354 SSYDRFCPFYKTV 392
+ ++ P YK V
Sbjct: 241 NQWELLNPVYKDV 253
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 29.1 bits (62), Expect = 0.54
Identities = 19/59 (32%), Positives = 28/59 (47%)
Frame = +3
Query: 228 QEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLK 404
QEE+D S+I + + +T + E KLL + L SY+ P Y + MLK
Sbjct: 35 QEEKDTSKITENAKEEVKRDTSSLAKESLKLLDLNGLNDESYTGDPGKGPEYTSSTMLK 93
>SPAC1B1.03c |kap95||karyopherin Kap95|Schizosaccharomyces pombe|chr
1|||Manual
Length = 863
Score = 28.3 bits (60), Expect = 0.95
Identities = 18/57 (31%), Positives = 27/57 (47%)
Frame = +3
Query: 129 LISYSKYMRALDDFYEKNYPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKI 299
L++ M +L++ +E P FVP + QE + S V LVG + A KI
Sbjct: 620 LLAIGAMMNSLEEQFEVYVPSFVPFLSSALSNEQEYQLCSVAVGLVGDLARALNAKI 676
>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1184
Score = 27.1 bits (57), Expect = 2.2
Identities = 20/69 (28%), Positives = 28/69 (40%)
Frame = +2
Query: 308 GRQAAQRRLPATEQLFVVRSLLPVLQDGGHAEEHHHVLRHVAARGGVHGAVRQQGHVERH 487
G A +R PAT + V+ G H + H +VAA+ G + R+Q E H
Sbjct: 18 GLHAMERYKPATSESLYVKG-----HYGPHPHKEHPAA-YVAAKTGFNATSRRQSVREHH 71
Query: 488 PRRHGQRAL 514
G L
Sbjct: 72 SSHEGSAQL 80
>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 772
Score = 26.6 bits (56), Expect = 2.9
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = -1
Query: 620 VAESGRHIF*KLVEIGLDLRFTVFHWVLELHGGELVEHVAHGVAD 486
V ES I+ L +G+DL FHW L ++ L +++ + D
Sbjct: 643 VKESLPEIYSHLELLGVDLDAISFHWFLSVYTDTLPTNISFRIFD 687
>SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein
Pkl1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 832
Score = 26.2 bits (55), Expect = 3.8
Identities = 11/49 (22%), Positives = 25/49 (51%)
Frame = +3
Query: 99 QRKHFPSINWLISYSKYMRALDDFYEKNYPEFVPLRTKVKEILQEEEDL 245
+ KH + L SKY+ ++ D N + L+ +++++ + ED+
Sbjct: 407 EHKHHSTSEKLEELSKYVASIQDKERNNGQNALELQARIQQLERRNEDM 455
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 26.2 bits (55), Expect = 3.8
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = -1
Query: 425 VVERDDVLQHAHRLVERAEAIV 360
+V+ DD LQ A++ VER E+++
Sbjct: 1 MVQLDDALQDAYKKVEREESLI 22
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.4 bits (53), Expect = 6.7
Identities = 14/48 (29%), Positives = 25/48 (52%)
Frame = -2
Query: 619 LRKAADISFKSWSKSALILGSPSFTGSLNFMEESW*STLPMASRMTFH 476
L +AA +S WS + L+L + S + L ++ + MA+ + FH
Sbjct: 153 LGQAAGVSSTDWSCAQLLLAAVSISTDLKYIPTNQHIVGVMAAVIVFH 200
>SPCC1259.11c |gyp2||GTPase activating protein Gyp2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 720
Score = 25.4 bits (53), Expect = 6.7
Identities = 7/24 (29%), Positives = 15/24 (62%)
Frame = -1
Query: 602 HIF*KLVEIGLDLRFTVFHWVLEL 531
H++ K++E+ +DL+ +W L
Sbjct: 360 HLYQKIIELDMDLKLITINWFFSL 383
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 25.0 bits (52), Expect = 8.8
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = -1
Query: 410 DVLQHAHRLVERAEAIVRRIAVLLQEVVFEQLGDLERDLV 291
D+LQ LV A +RR A+ + ++ D++RD +
Sbjct: 710 DILQLVLPLVAVPNAYIRRAALSVIYSAYQSFDDIDRDCI 749
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,926,275
Number of Sequences: 5004
Number of extensions: 35546
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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