Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV31883
(704 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 39 8e-04
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 33 0.040
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 33 0.040
SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 31 0.16
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 27 2.0
SPBC29A3.12 |rps902|rps9-2, rps9b|40S ribosomal protein S9|Schiz... 27 2.6
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 2.6
SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1 |S... 27 3.5
SPAC27D7.12c |but1|SPAC27D7.12, mug107|neddylation pathway prote... 26 4.6
SPAC25B8.09 |||trans-aconitate 3-methyltransferase |Schizosaccha... 26 6.0
SPAC24H6.07 |rps901|rps9-1, rps9a|40S ribosomal protein S9|Schiz... 26 6.0
SPAC11D3.18c |||nicotinic acid plasma membrane transporter |Schi... 26 6.0
SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual 25 8.0
>SPCC191.09c |gst1||glutathione S-transferase
Gst1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 229
Score = 38.7 bits (86), Expect = 8e-04
Identities = 24/58 (41%), Positives = 33/58 (56%), Gaps = 3/58 (5%)
Frame = +1
Query: 160 LKTMKYDFEPKYVNLRKAEHYSEDFTKVNRMQRVP-VIDH--NGFILTESIAILKYLS 324
LK + +E +YVN K E S + +N RVP +IDH N + + ES AIL YL+
Sbjct: 22 LKELDLTYETRYVNFSKNEQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLA 79
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 33.1 bits (72), Expect = 0.040
Identities = 25/102 (24%), Positives = 54/102 (52%)
Frame = +1
Query: 103 VLKLYYDLMSQPSRVLYILLKTMKYDFEPKYVNLRKAEHYSEDFTKVNRMQRVPVIDHNG 282
++ L++ S+ +R+++ +L+ +K +E K + R +TK++ + + P++ +G
Sbjct: 1 MIVLHHLKNSRSTRIVW-MLEELKVPYEIKVYD-RVDGRAPPAYTKLSPLGKSPIVVDDG 58
Query: 283 FILTESIAILKYLSRENIIAESLYSKESKLQARIEEFLEWQH 408
ES AIL++L R+ S E + A +E++ W H
Sbjct: 59 VTYIESAAILEHLVRK--YGPSFKPSEEDV-AELEKYELWMH 97
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 33.1 bits (72), Expect = 0.040
Identities = 37/171 (21%), Positives = 81/171 (47%), Gaps = 1/171 (0%)
Frame = +1
Query: 118 YDLMSQPSRVLYILLKTMKYDFEPKYVNLRKAEHYSEDFTKVNRMQRVPV-IDHNGFILT 294
Y + P RVL+ + E ++V+++ ++ D +Q++PV + +GF L+
Sbjct: 7 YGKIGSP-RVLFCVSVAAVAGVEVEHVDVQP-HNFPADLAAKFPLQKMPVFVGKDGFPLS 64
Query: 295 ESIAILKYLSRENIIAESLYSKESKLQARIEEFLEWQHIGLRLHCAMYFRVVHMDPILTG 474
E++AI YL+ N +L ++ +A++ ++ + + L FR + + P + G
Sbjct: 65 ETLAIAFYLASLN-KTRALNGTTAEEKAKVLQYCSFTNSEL----PGAFRPI-IAPRVFG 118
Query: 475 RKSDEKTIQGYKRRMMMALDDLDTKWLGRGTAFIVGETPTVADLVAACELE 627
DE+ + + + + D + + ++VG T+AD+ C L+
Sbjct: 119 APYDEQAAKEAETAIALIFARFDEELASK--TYLVGSRLTLADIFFTCFLK 167
>SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 396
Score = 31.1 bits (67), Expect = 0.16
Identities = 24/99 (24%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
Frame = +1
Query: 241 VNRMQRVPVIDHNGFILTESIAILKYLSRENIIAESLY-SKESKLQARIEEFLEWQHIGL 417
V+R+QR P + G + T + +++ N + SLY S E K++ QH+
Sbjct: 215 VHRVQRTPATETKGRVHTSTASVIVLPQVSNDESSSLYDSSEVKIEVMRSRGAGGQHVN- 273
Query: 418 RLHCAMYFRVVHMDPILTGRKSDEKTIQGYKRRMMMALD 534
R A+ R+ H+ +T D ++ K + + L+
Sbjct: 274 RTESAV--RLTHIPTGITVSMQDSRSQHQNKEKAFLVLN 310
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 27.5 bits (58), Expect = 2.0
Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +1
Query: 220 YSEDFTKVNRMQRVPV-IDHNGFILTESIAILKY 318
+S D +Q++PV I +GF L+E IAI+KY
Sbjct: 39 FSADLAAKFPLQKLPVFIGADGFELSEVIAIVKY 72
>SPBC29A3.12 |rps902|rps9-2, rps9b|40S ribosomal protein
S9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 192
Score = 27.1 bits (57), Expect = 2.6
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = +1
Query: 289 LTESIAILKYLSRENIIAESLYSKESKLQARIEEFLE 399
L E AI++ L R I+ ES + L RIE+FLE
Sbjct: 70 LFEGNAIIRRLVRLGILDESRMKLDYVLALRIEDFLE 106
>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 27.1 bits (57), Expect = 2.6
Identities = 16/54 (29%), Positives = 25/54 (46%)
Frame = -1
Query: 692 FGHHAAMFGK*SLGSKPAILGCSNSQAATKSATVGVSPTINAVPLPSHLVSKSS 531
FG ++ GK ++ + NS A SP+IN+VP S++V S
Sbjct: 267 FGDKSSTAGKHNVETTSPPSSSPNSDDAYSPQVDSYSPSINSVPYTSNIVENPS 320
>SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 445
Score = 26.6 bits (56), Expect = 3.5
Identities = 17/66 (25%), Positives = 33/66 (50%)
Frame = +1
Query: 154 ILLKTMKYDFEPKYVNLRKAEHYSEDFTKVNRMQRVPVIDHNGFILTESIAILKYLSREN 333
I+ M+Y+ E ++ + + F+ + ++ VP + FI+ + + LKYL +N
Sbjct: 218 IIKVNMEYNSETQFFIFEEMVTGGDLFSYLTKLGTVPEVT-TLFIMFQILQGLKYLHEQN 276
Query: 334 IIAESL 351
II L
Sbjct: 277 IIHRDL 282
>SPAC27D7.12c |but1|SPAC27D7.12, mug107|neddylation pathway protein
But1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 243
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/41 (26%), Positives = 23/41 (56%)
Frame = +1
Query: 169 MKYDFEPKYVNLRKAEHYSEDFTKVNRMQRVPVIDHNGFIL 291
M+ FEP ++NL K + +N + ++P++ + F+L
Sbjct: 68 MEVVFEPCFINLSKPVYVVNGIGGINEIHKLPIL-FSSFVL 107
>SPAC25B8.09 |||trans-aconitate 3-methyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 251
Score = 25.8 bits (54), Expect = 6.0
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +1
Query: 544 TKWLGRGTAFIVGETPTVADLVAACELEQPRMAGFEPKD 660
T+WL F V ET T+ +L A PR+ +PK+
Sbjct: 25 TEWLN--DEFSVNETSTILELGAGSGKLTPRIIASQPKE 61
>SPAC24H6.07 |rps901|rps9-1, rps9a|40S ribosomal protein
S9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 191
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +1
Query: 289 LTESIAILKYLSRENIIAESLYSKESKLQARIEEFLE 399
L E AI++ L R I+ E+ + L RIE+FLE
Sbjct: 70 LFEGNAIIRRLVRLGILDETRMKLDYVLALRIEDFLE 106
>SPAC11D3.18c |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = -3
Query: 462 WIHMHNSEIHGTVQTKTYMLPLKEFFNPGLKLGFL 358
+I M S+ + T TY++ + + PGL LG+L
Sbjct: 367 YIIMLASDSNAAKYTATYIIAIGCYIGPGLNLGWL 401
>SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 533
Score = 25.4 bits (53), Expect = 8.0
Identities = 17/59 (28%), Positives = 29/59 (49%)
Frame = +1
Query: 214 EHYSEDFTKVNRMQRVPVIDHNGFILTESIAILKYLSRENIIAESLYSKESKLQARIEE 390
E+Y++ + V + VPV + A +L++ NIIAE K+S L A + +
Sbjct: 112 EYYAKTGSLVGPLHGVPVSVKEHISIKNHTATASFLAKANIIAE----KDSDLVATVRK 166
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,635,867
Number of Sequences: 5004
Number of extensions: 50787
Number of successful extensions: 155
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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