Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV31814
(479 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 47 2e-06
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 37 0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 35 0.007
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 33 0.017
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 31 0.090
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.1
SPAC23H4.04 |||tRNA|Schizosaccharomyces pombe|chr 1|||Manual 27 1.1
SPCC550.13 |dfp1|rad35, him1|Hsk1-Dfp1 kinase complex regulatory... 26 3.4
SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pomb... 25 4.5
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 25 4.5
SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S... 25 5.9
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 25 5.9
SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF... 25 7.8
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 46.8 bits (106), Expect = 2e-06
Identities = 34/111 (30%), Positives = 55/111 (49%), Gaps = 7/111 (6%)
Frame = +1
Query: 166 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALW-K 342
F D+D+ G I+++EL + + +P E ++ MI D G I F +F + +
Sbjct: 18 FSLFDRDQDGNITSNELGVVMRSLGQSPTAAE-LQDMINEVDADGNGTIDFTEFLTMMAR 76
Query: 343 YVSDWQN------CFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMV 477
+ D N F+ FD+D +G I EL + LT+ G RLS + V M+
Sbjct: 77 KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMI 127
Score = 27.1 bits (57), Expect = 1.5
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 346 VSDWQNCFRSFDRDNSGNIDRVELKNALTAFG 441
+++++ F FDRD GNI EL + + G
Sbjct: 11 IAEFREAFSLFDRDQDGNITSNELGVVMRSLG 42
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 36.7 bits (81), Expect = 0.002
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +1
Query: 352 DWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMV 477
++ FR FD+DNSG I+ + + + G +LSD+ V +MV
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMV 120
Score = 30.3 bits (65), Expect = 0.16
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +1
Query: 166 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDF 327
FR DKD SGYI + + + V+LM+ D N G + DF
Sbjct: 84 FRVFDKDNSGYIETAKFADYMKT-LGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 34.7 bits (76), Expect = 0.007
Identities = 27/111 (24%), Positives = 46/111 (41%), Gaps = 7/111 (6%)
Frame = +1
Query: 166 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALW-- 339
F+ D D+ I EL+ A+ +N E ++++ FDK +G + EDF +
Sbjct: 43 FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD-FDKTGKGYLQMEDFVRVMTE 101
Query: 340 KYV-----SDWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIMV 477
K V + + F FD D +G I L+ + D + M+
Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMI 152
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 33.5 bits (73), Expect = 0.017
Identities = 19/67 (28%), Positives = 35/67 (52%)
Frame = +1
Query: 124 FQSPMPSRDFLWNIFRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNR 303
F P ++++N+F D D++GYI E ALS + N + + ++D N
Sbjct: 58 FGDPSAFAEYVFNVF---DADKNGYIDFKEFICALSVTSRGELNDKLI-WAFQLYDLDNN 113
Query: 304 GVISFED 324
G+IS+++
Sbjct: 114 GLISYDE 120
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 31.1 bits (67), Expect = 0.090
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = +1
Query: 346 VSDWQNCFRSFDRDNSGNIDRVELKNALTAFGYRLSDDVVGIM 474
+ + + F D+D GNI R ++K LT+ S+D + M
Sbjct: 47 IQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHM 89
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.5 bits (58), Expect = 1.1
Identities = 17/62 (27%), Positives = 31/62 (50%)
Frame = +1
Query: 166 FRSVDKDRSGYISADELQQALSNGTWNPFNPETVRLMIGMFDKQNRGVISFEDFGALWKY 345
F D GYI ++L+++ + N E ++LM+ + G +S E+F LW +
Sbjct: 18 FDLFDVTHKGYIDFEDLRRSCAQLGEN-LTKEQLQLMLDLAG--TNGKVSREEFAELWIH 74
Query: 346 VS 351
+S
Sbjct: 75 IS 76
>SPAC23H4.04 |||tRNA|Schizosaccharomyces pombe|chr 1|||Manual
Length = 415
Score = 27.5 bits (58), Expect = 1.1
Identities = 16/56 (28%), Positives = 23/56 (41%), Gaps = 8/56 (14%)
Frame = +2
Query: 41 CEGIKYYCARVAFHKVYW-VVFFPRLK*LSNR-------QCQAEISYGTFLEVLTK 184
C+ + C R F K YW +VF P L N C ++ +G + L K
Sbjct: 89 CKKLNISCRRFNFEKEYWNLVFEPSLDLYENGLTPNPDVSCNRQVKFGALFDALKK 144
>SPCC550.13 |dfp1|rad35, him1|Hsk1-Dfp1 kinase complex regulatory
subunit Dfp1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 545
Score = 25.8 bits (54), Expect = 3.4
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = +1
Query: 106 P*IKMTFQSPMPSRDFLWNIFRSVDKDRSGYI 201
P + +Q P+P RD W FR+ R ++
Sbjct: 293 PILLREWQKPLPDRDVPWPTFRATSIGRCPFV 324
>SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 431
Score = 25.4 bits (53), Expect = 4.5
Identities = 13/49 (26%), Positives = 20/49 (40%)
Frame = -1
Query: 476 TIIPTTSSDNLYPKAVSAFFNSTLSIFPELSRSNDRKQFCQSETYFQSA 330
TIIP + A + T S+ + ++ D Q C TY + A
Sbjct: 263 TIIPIFLDPSYQSYAAKTIHSDTSSLASSIIQTGDGTQSCDESTYIELA 311
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 25.4 bits (53), Expect = 4.5
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 309 DTPILFVKHTNHQTYSF 259
D PILF+KH N+ T F
Sbjct: 216 DLPILFIKHPNNGTSFF 232
>SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1206
Score = 25.0 bits (52), Expect = 5.9
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = -1
Query: 284 IPIIRRTVSGLNGFHVPLDRACCSSSADM*PLLSLSTLLKM 162
IPI+RR + N P ++ S+SA+ PL+ + L KM
Sbjct: 274 IPILRRQAASANAISTPWNQV-NSNSANDGPLIVSAVLHKM 313
>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 25.0 bits (52), Expect = 5.9
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -2
Query: 109 REKNNPINFMKCHTCTIIFYTFTLEQENN 23
+EKN NF + YTFTLE + N
Sbjct: 518 KEKNEKYNFTSRKRLGLSPYTFTLENQLN 546
>SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease
XPF|Schizosaccharomyces pombe|chr 3|||Manual
Length = 892
Score = 24.6 bits (51), Expect = 7.8
Identities = 6/28 (21%), Positives = 18/28 (64%)
Frame = -3
Query: 345 IFPKCTKVLK*YDTPILFVKHTNHQTYS 262
++ +C + + Y+ P+L ++ HQ+++
Sbjct: 725 LYSQCEAMTEYYEIPVLLIEFEQHQSFT 752
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,311,543
Number of Sequences: 5004
Number of extensions: 51396
Number of successful extensions: 148
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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