Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV31433
(690 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 111 9e-26
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 109 4e-25
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 91 1e-19
SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity ... 60 3e-10
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 33 0.029
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 29 0.63
SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 29 0.84
SPAC4D7.12c |fet5||transcription factor Fet5|Schizosaccharomyces... 28 1.5
SPAC1782.11 |met14||adenylyl-sulfate kinase |Schizosaccharomyces... 27 1.9
SPCC188.06c |srp54||signal recognition particle subunit Srp54|Sc... 26 4.5
SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces pombe... 26 5.9
>SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 592
Score = 111 bits (267), Expect = 9e-26
Identities = 53/132 (40%), Positives = 81/132 (61%), Gaps = 3/132 (2%)
Frame = +3
Query: 210 VIAMVGLPARGKTYISKKLSRYLNWIGINTRVFNLGEYRR-HATSAYT-SHEFFRADNKE 383
+I +VG+PA GK+YI KLSRY NW+ N R F++G+ RR S Y+ S +FF N+E
Sbjct: 145 IIILVGIPATGKSYIGSKLSRYYNWLKYNCRFFSVGDKRREEGASTYSMSADFFDIKNEE 204
Query: 384 AMAIRQQCALXALQDVCEWLT-KGGEVAVFDATNSTLDRRRMIRDLVVHKMGFKLFFVES 560
R+ AL L+D+ W+ + G + + DATNST +RR+ + D + + + F+ES
Sbjct: 205 TFKFRENVALETLEDLLHWMIHENGVIGILDATNSTHERRKHLYDRISKEADIGIMFLES 264
Query: 561 ICDDPXIIEQNI 596
+C D + E+NI
Sbjct: 265 LCTDDILFEENI 276
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 109 bits (262), Expect = 4e-25
Identities = 55/128 (42%), Positives = 77/128 (60%), Gaps = 2/128 (1%)
Frame = +3
Query: 219 MVGLPARGKTYISKKLSRYLNWIGINTRVFNLGEYRR-HATSAYTSHEFFRADNKEAMAI 395
MVGLPARGK+YI KL RY NW+ N + FN+G +RR HA+ ++ FF N+EA +
Sbjct: 1 MVGLPARGKSYIVNKLVRYYNWLQYNCKAFNVGNFRRKHASHSHDDASFFDPSNEEASKL 60
Query: 396 RQQCALXALQDVCEWL-TKGGEVAVFDATNSTLDRRRMIRDLVVHKMGFKLFFVESICDD 572
R+ A+ L + +W + G V +FDATNST RR+ I D + F+ESIC+D
Sbjct: 61 RESFAMDTLDALLQWFEEEDGVVGIFDATNSTSKRRKAIVDHLSKVPYVTTLFIESICND 120
Query: 573 PXIIEQNI 596
+I N+
Sbjct: 121 EQLIAANM 128
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 91.5 bits (217), Expect = 1e-19
Identities = 51/132 (38%), Positives = 73/132 (55%), Gaps = 1/132 (0%)
Frame = +3
Query: 210 VIAMVGLPARGKTYISKKLSRYLNWIGINTRVFNLGEYRRHATSAYTSHEFFRADNKEAM 389
VI M GLPARGK+ I+ + RYL W+G N R ++L +Y T TS A ++ +
Sbjct: 54 VIIMSGLPARGKSNIAVSIDRYLRWLGFNCRFYSLAKYIDERTREMTSSPVKSAASENHV 113
Query: 390 AIRQQCALXALQDVCEWLTK-GGEVAVFDATNSTLDRRRMIRDLVVHKMGFKLFFVESIC 566
R L D+ +L K G+VA++DATN T RR++ D GFK+ F+ES+C
Sbjct: 114 FSRNDTIERCLADLEIFLLKEKGQVAIYDATNGTRRTRRILYDR-FKNCGFKILFIESLC 172
Query: 567 DDPXIIEQNIME 602
+ +I NI E
Sbjct: 173 NKEDVINANIQE 184
>SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 408
Score = 60.1 bits (139), Expect = 3e-10
Identities = 41/131 (31%), Positives = 64/131 (48%)
Frame = +3
Query: 213 IAMVGLPARGKTYISKKLSRYLNWIGINTRVFNLGEYRRHATSAYTSHEFFRADNKEAMA 392
+ VGLPA GKT + KLSRYL W+ ++T +++ E + S + E +A
Sbjct: 12 VCFVGLPASGKTSSAMKLSRYLTWMSVSTHLYDTKEIDNSLSENSNS-------DSENLA 64
Query: 393 IRQQCALXALQDVCEWLTKGGEVAVFDATNSTLDRRRMIRDLVVHKMGFKLFFVESICDD 572
L+ V KG +VA+ D TL R+ I +L + L FVE +C +
Sbjct: 65 KTLSPIFDNLESV---FKKGTDVAILDFNQCTLKFRKSIVELAKAR-NMLLMFVEVVCTN 120
Query: 573 PXIIEQNIMEV 605
II++NI ++
Sbjct: 121 QKIIDENITDM 131
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 33.5 bits (73), Expect = 0.029
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = +3
Query: 399 QQCALXALQDVCEWLTKGGEVAVFDATNSTLDRRRMIRDLVVHKMGFKLF 548
+Q L A++DV EWL GE DA+ + +R D VVH + KL+
Sbjct: 596 KQAVLDAVEDVAEWLEIHGE----DASKEEFEDQRQKLDAVVHPITQKLY 641
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 29.1 bits (62), Expect = 0.63
Identities = 21/84 (25%), Positives = 37/84 (44%)
Frame = +3
Query: 321 YRRHATSAYTSHEFFRADNKEAMAIRQQCALXALQDVCEWLTKGGEVAVFDATNSTLDRR 500
+R H S + S F++ +I +C L L C W E+ D + + +R
Sbjct: 1591 FRPHEISRFNSQSVFKSKKHHLKSIVVKCTLQLLMLNCLW-----ELFHSDNMLTNIPKR 1645
Query: 501 RMIRDLVVHKMGFKLFFVESICDD 572
+M++ L + K ++ F ES D
Sbjct: 1646 KMVKLLDILKQSWE--FAESFNSD 1667
>SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 511
Score = 28.7 bits (61), Expect = 0.84
Identities = 18/71 (25%), Positives = 35/71 (49%), Gaps = 1/71 (1%)
Frame = -2
Query: 359 LMACVCGCSMSSVFA*IKYSCIDPYPVQVA*QFLGNVCLPSCRETHHCDNMWYV-DIIRS 183
++ CV C F+ ++YS A + + N+ +C+E + +N + DI+ +
Sbjct: 331 MLTCVSPCDFDDTFSALRYS--------EAARRIKNISNINCKEAYSTNNEGELDDILTT 382
Query: 182 FSSDRERFRHN 150
SDRE+ R +
Sbjct: 383 LESDREQLRRH 393
>SPAC4D7.12c |fet5||transcription factor Fet5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 276
Score = 27.9 bits (59), Expect = 1.5
Identities = 14/27 (51%), Positives = 17/27 (62%)
Frame = +3
Query: 15 LNGSPSLRLRGGINNNSDSKKHELNQC 95
LN P L L G IN ++ K HELN+C
Sbjct: 193 LNTDPLL-LTGEINETTNPKFHELNRC 218
>SPAC1782.11 |met14||adenylyl-sulfate kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 202
Score = 27.5 bits (58), Expect = 1.9
Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
Frame = +3
Query: 180 ERSNYVNIPHV-IAMVGLPARGKTYISKKLSRYLNWIGINT 299
ER +V P + I M GL A GK+ I+ L +YL G+ T
Sbjct: 16 ERIKFVGHPGMTIWMTGLSASGKSTIACALEQYLLQRGVTT 56
>SPCC188.06c |srp54||signal recognition particle subunit
Srp54|Schizosaccharomyces pombe|chr 3|||Manual
Length = 522
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +3
Query: 204 PHVIAMVGLPARGKTYISKKLSRYLNWIGINTRVFNLGEYRRHA 335
P VI MVGL GKT KL+ + G+ + + +R A
Sbjct: 101 PSVIMMVGLQGSGKTTTCSKLALHYQRRGLKSCLVAADTFRAGA 144
>SPBC17D1.03c |||exosome subunit Rrp43 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 270
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = -3
Query: 397 LIAMASL-LSALKNSWLVYADVACLLY 320
LI + SL + K +W++YAD+ CL Y
Sbjct: 130 LINLQSLCIFEKKAAWVLYADIICLNY 156
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,880,918
Number of Sequences: 5004
Number of extensions: 59231
Number of successful extensions: 196
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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