Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= maV31342
         (657 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyce...    63   3e-11
SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr 1|...    33   0.036
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe...    29   0.45 
SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr 2...    26   5.5  
SPCP25A2.02c |rhp26||SNF2 family helicase Rhp26|Schizosaccharomy...    25   7.3  
SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces...    25   7.3  
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa...    25   9.6  
SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyc...    25   9.6  

>SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 520

 Score = 63.3 bits (147), Expect = 3e-11
 Identities = 56/186 (30%), Positives = 84/186 (45%), Gaps = 19/186 (10%)
 Frame = +2

Query: 152 GQLQGRIVNSPSGKA-FYSFQGIPYAKPPLGSLRFKAPQSPEP-WDGIRDATAEGNVCAQ 325
           G L G  V   +GK   + F GI YAKPP+G LR++ P + E  +D   D      +C Q
Sbjct: 2   GVLHGLTVLDENGKEKCHRFTGIRYAKPPVGKLRWRRPVTLEDGYDYSGDYNQFKTICPQ 61

Query: 326 ------IDPVFAKSYVGDENCLFLNVYTPSTD--GAFLPVMIWIHGGGFKWGSG-NTNLY 478
                  + V    +  DE+CLFLN++ P+ +      PV+ +IHGG  + G+  +    
Sbjct: 62  PFYNNRKNQVRNPDFKYDEDCLFLNIWVPAGEKPAEGWPVLYFIHGGWLQVGNPLHYRQC 121

Query: 479 GP-DFLVD---RDVVVMTINYRCGALGFLS----LNTPEVPGNAGIKDIVQAIRWVKDNI 634
            P D   D      ++++  +R    GFL+    L       N G  D    + W   +I
Sbjct: 122 DPQDLQADGSPAKFILVSPGHRLNLFGFLAGKELLEEDPKSSNFGFWDQRLGLEWTYKHI 181

Query: 635 HHFGGN 652
             FGGN
Sbjct: 182 ESFGGN 187


>SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 341

 Score = 33.1 bits (72), Expect = 0.036
 Identities = 24/80 (30%), Positives = 35/80 (43%), Gaps = 1/80 (1%)
 Frame = +2

Query: 416 PVMIWIHGGGFKWGSGNT-NLYGPDFLVDRDVVVMTINYRCGALGFLSLNTPEVPGNAGI 592
           P  +W HGGG+  G+ NT N +          VV+ ++YR           PE P  A I
Sbjct: 101 PCFLWFHGGGWVLGNINTENSFATHMCEQAKCVVVNVDYRL---------APEDPFPACI 151

Query: 593 KDIVQAIRWVKDNIHHFGGN 652
            D  +A+ +  +N    G N
Sbjct: 152 DDGWEALLYCYENADTLGIN 171


>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 230

 Score = 29.5 bits (63), Expect = 0.45
 Identities = 14/40 (35%), Positives = 19/40 (47%)
 Frame = +2

Query: 218 PYAKPPLGSLRFKAPQSPEPWDGIRDATAEGNVCAQIDPV 337
           P   P  G      P+SP  + G+ DA+ EGN    ID +
Sbjct: 3   PSTPPMRGLCTPSTPESPGSFKGVIDASLEGNSSIMIDEI 42


>SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 413

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 11/31 (35%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
 Frame = +2

Query: 566 PEVPGNAGIKD-IVQAIRWVKDNIHHFGGNA 655
           P +P  + + D ++ ++R V  +I HFGG+A
Sbjct: 362 PSIPKVSVMDDTLIDSVRGVPGDISHFGGDA 392


>SPCP25A2.02c |rhp26||SNF2 family helicase Rhp26|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = +3

Query: 294 MPRRKGTCVLKSIQSSRNPMSAMKTAC 374
           +PR  G CVL      RNP S +  +C
Sbjct: 431 LPREWGYCVLDEGHKIRNPDSEISISC 457


>SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 591

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +2

Query: 275 PWDGIRDATAEGNVCAQIDPVFAKSYVGDENCLFL 379
           P + IRD     N   ++DP FAK+YV     LF+
Sbjct: 449 PAECIRDC----NKAIELDPNFAKAYVRKAQALFM 479


>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 669

 Score = 25.0 bits (52), Expect = 9.6
 Identities = 8/19 (42%), Positives = 11/19 (57%)
 Frame = -3

Query: 628 IFHPSNCLNYIFDPSIPWD 572
           IF   N +    DP++PWD
Sbjct: 261 IFFDKNTIKPFHDPTVPWD 279


>SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 835

 Score = 25.0 bits (52), Expect = 9.6
 Identities = 13/37 (35%), Positives = 17/37 (45%), Gaps = 1/37 (2%)
 Frame = -3

Query: 412 ECSISARGVH-V*EQAVFIADIGFREDWIDLSTHVPF 305
           EC  S  GV  + +   FI+  GF E W      +PF
Sbjct: 137 ECQFSEGGVFALLQNDTFISITGFEEPWRKTYASIPF 173


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,112,995
Number of Sequences: 5004
Number of extensions: 73068
Number of successful extensions: 195
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)