Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV31173
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 63 3e-11
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 54 2e-08
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 51 1e-07
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 46 4e-06
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 36 0.008
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 35 0.013
SPAC1783.02c |vps66||acyltransferase |Schizosaccharomyces pombe|... 28 1.2
SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyc... 28 1.5
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 28 1.5
SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces ... 27 2.7
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 26 6.1
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 25 8.1
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 25 8.1
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 63.3 bits (147), Expect = 3e-11
Identities = 43/178 (24%), Positives = 81/178 (45%), Gaps = 10/178 (5%)
Frame = +3
Query: 162 LIFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINSAYS 341
L F ++ + + A A+++LG+ KGD + + +L T A L T+
Sbjct: 97 LSFNDIYDKALRYAGALRKLGLNKGDKFELYAPTSAFWLLTAEACLSQSMTIVTAYDTLG 156
Query: 342 KSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFFLFGISDDKDVTSFKDLVT-- 515
+ +H+ + S ++ K LK++ ++ + ++D+ + +++
Sbjct: 157 EEGLLHSLRESGVRGMYTEGHLLKTLVNPLKEIESLEVIIYRNDAKEEDIKTIQEIRPNL 216
Query: 516 KYTHFNDFESN------DFNGTDSTSIILYSSGTTGLPKGVKLTHRNL--LVTGFQNH 665
K F DFE D + I+Y+SG+TGLPKGV L+H+N+ +VT H
Sbjct: 217 KLIKFADFEKMSPPVEPDPPSPEEICCIMYTSGSTGLPKGVILSHKNMVAIVTAIVKH 274
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 54.0 bits (124), Expect = 2e-08
Identities = 44/158 (27%), Positives = 73/158 (46%), Gaps = 10/158 (6%)
Frame = +3
Query: 198 IASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINSAYSKSEFIHTTKISK 377
+A + + GI +GDVV + + + + + VL GAT + I+ AY + I ++K
Sbjct: 325 LAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQIIYLSVAK 384
Query: 378 PNYIF-------LSPSA--YKEFYVTLKQLNIVQKFFLFGISDDKDVTSFKDLVTKYT-H 527
P + LSP+ Y E + LK K G V+ D + ++ H
Sbjct: 385 PRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSKGADDILQHVLH 444
Query: 528 FNDFESNDFNGTDSTSIILYSSGTTGLPKGVKLTHRNL 641
++ G DST + ++SG+ G+PKGVK H +L
Sbjct: 445 LKSEQTGVVVGPDSTPTLSFTSGSEGIPKGVKGRHFSL 482
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 51.2 bits (117), Expect = 1e-07
Identities = 46/175 (26%), Positives = 73/175 (41%), Gaps = 5/175 (2%)
Frame = +3
Query: 132 ALINGASGEKLIFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGA 311
AL+ + +L F E+ I+++ I LGI GD V I N E++ AV A
Sbjct: 18 ALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQRA 77
Query: 312 TVTFINSAYSKSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFFLFGISDDKDV 491
+NS Y +SEF K + + + + +K ++ +
Sbjct: 78 ICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVA---ANTPAVRAAKK---LSVAVAELA 131
Query: 492 TSFKDLVTKYTHFNDFESN-----DFNGTDSTSIILYSSGTTGLPKGVKLTHRNL 641
K + + HF + N D ++L++SGTTG PK V LTH+NL
Sbjct: 132 WCPKSRLVRIVHFEGAKINAPQPLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKNL 186
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 46.4 bits (105), Expect = 4e-06
Identities = 42/172 (24%), Positives = 79/172 (45%), Gaps = 2/172 (1%)
Frame = +3
Query: 159 KLIFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINSAY 338
+L + E+ ++ +A +K G G ++A+ + E + +A+L G ++ +
Sbjct: 2751 RLTYSELNERANKLAHNLKSYGFRVGSIIAVYFDKCIEAFISMLAILKAGCCFLALDVS- 2809
Query: 339 SKSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFFLFGISDDKDVTSFKDLVTK 518
+ +E I + +S E Y L LN + SD + ++ ++
Sbjct: 2810 APTERIRYIVTDSTAVLVMSTG---ELYTKL--LNASINVTILDASDPGNYSN--NIENP 2862
Query: 519 YTHFNDFESNDFNGTDSTSIILYSSGTTGLPKGVKLTHRNLL--VTGFQNHF 668
YT DFE ++ + +LY+SG+TG PKG LTH N++ + FQ+ F
Sbjct: 2863 YT--KDFEDSNL------AYVLYTSGSTGKPKGCCLTHHNVVQCMLAFQDQF 2906
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/24 (58%), Positives = 20/24 (83%)
Frame = +3
Query: 564 DSTSIILYSSGTTGLPKGVKLTHR 635
DS + +LY+SG+TG PKGV ++HR
Sbjct: 359 DSVAYVLYTSGSTGNPKGVAISHR 382
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 210 IKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGA 311
+ +GI K DVVA+ + +Y + + VL GA
Sbjct: 1797 LHSIGIKKNDVVAVFIDKSLDYYSLMLGVLRIGA 1830
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 35.5 bits (78), Expect = 0.008
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = +3
Query: 564 DSTSIILYSSGTTGLPKGVKLTHRNLL 644
D +Y+SG+TG PKGV L HRN++
Sbjct: 245 DDICCYMYTSGSTGKPKGVVLLHRNII 271
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 34.7 bits (76), Expect = 0.013
Identities = 36/173 (20%), Positives = 72/173 (41%), Gaps = 17/173 (9%)
Frame = +3
Query: 153 GEKLIFGEMTQQIVNIASAIKQLGIGKGDVVAICSENRTEYLTTTIAVLCTGATVTFINS 332
G + + E+ + A A++ +G+G GD VAI E + +A++ GA + I +
Sbjct: 115 GRYITYRELLASVSQCAGALQSMGVGMGDRVAIYMPMIPETIIAMLAIVRLGAIHSVIFA 174
Query: 333 AYSKSEFIHTTKISKPNYIFLSPSAYK---------EFYVTLKQLNIVQKFFLF------ 467
+S S+ I + +++ L + ++K +F
Sbjct: 175 GFSAESVADRVNDSECKVIITADESHRGGKRIPLKGVVNKALTECPTIKKVLVFQRSAEP 234
Query: 468 --GISDDKDVTSFKDLVTKYTHFNDFESNDFNGTDSTSIILYSSGTTGLPKGV 620
+ + +DV + D++ K+ + + +LY+SG+TG PKGV
Sbjct: 235 TASMVEGRDVW-WHDIIPKFPRYC---PPAVVNPEHPLFLLYTSGSTGKPKGV 283
>SPAC1783.02c |vps66||acyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 328
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -2
Query: 365 SCMYEFTFAICTVYESNRSSCTKHSYSCSQILCP 264
SC+Y TFA+C SN S + +Y S P
Sbjct: 153 SCLYNCTFAVCDSKTSNVSIISAQAYFWSCFFSP 186
>SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1088
Score = 27.9 bits (59), Expect = 1.5
Identities = 20/89 (22%), Positives = 34/89 (38%), Gaps = 2/89 (2%)
Frame = +3
Query: 300 CTGATVTFINSAYSKSEFIHTTKISKPNYIFLSPSAYKEFYVTLKQLNIVQKFF--LFGI 473
C + S + E I T + S YK+ LK+LN + F +
Sbjct: 132 CNSRIIALKQSLEASQECIGNTNSKELQQTLARSSQYKKVISVLKELNEANQLFDNFHTL 191
Query: 474 SDDKDVTSFKDLVTKYTHFNDFESNDFNG 560
D K DL+ + +++ +DF+G
Sbjct: 192 VDSKQYYHASDLIRRV--WDELSRSDFDG 218
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 27.9 bits (59), Expect = 1.5
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = -2
Query: 188 LLCHFAKYQFLSASSIDQSHFCFFFRKSLQ*KFSI 84
LL + +KY++++ ID++ F FF K ++ S+
Sbjct: 81 LLLYKSKYEYITWDLIDENRFQLFFEKVIEVSLSL 115
>SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 343
Score = 27.1 bits (57), Expect = 2.7
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -1
Query: 99 IKIFHNSCDSETKNFALHKLYPHFAFLFPFCTS 1
I I NS +E K+YP F L+PF TS
Sbjct: 217 ITITINSFGTELSKEERAKMYPSFGRLYPFSTS 249
>SPAC328.04 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 25.8 bits (54), Expect = 6.1
Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = -3
Query: 292 AIVVVRYSVLFSLQIAT-TSPFPMPSCLIAEAIFTICCVISPNINF 158
A+ SV FS+ ++ TS F S + A+FT+ +SP+I F
Sbjct: 511 AVATESRSVFFSISASSLTSKFLGESEKLVRALFTLAKKLSPSIIF 556
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 25.4 bits (53), Expect = 8.1
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +3
Query: 69 PNHMNYGKFLL-ERLSKEKAKVALINGASGEKLIFGEMTQQIVNIASAIK 215
PN Y K L ERL+K +A+I ++ E +IV+ +A+K
Sbjct: 387 PNLTEYEKEKLQERLAKLSGGIAVIKVGGSSEVEVNEKKDRIVDALNAVK 436
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/31 (35%), Positives = 15/31 (48%)
Frame = -2
Query: 215 FNSRSYIYNLLCHFAKYQFLSASSIDQSHFC 123
F+ S + + HF QF S S I +H C
Sbjct: 264 FSGDSSLKSFSLHFFALQFFSTSLIQYTHIC 294
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,055,266
Number of Sequences: 5004
Number of extensions: 66378
Number of successful extensions: 221
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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