Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30828
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 192 1e-49
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 100 8e-22
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 98 5e-21
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 98 5e-21
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 98 5e-21
At2g27035.1 68415.m03248 plastocyanin-like domain-containing pro... 30 1.2
At5g28760.1 68418.m03532 hypothetical protein contains Pfam prof... 29 2.8
At1g75800.1 68414.m08805 pathogenesis-related thaumatin family p... 29 2.8
At1g66310.1 68414.m07530 F-box family protein contains F-box dom... 29 3.8
At1g56120.1 68414.m06444 leucine-rich repeat family protein / pr... 29 3.8
At1g18485.1 68414.m02307 pentatricopeptide (PPR) repeat-containi... 29 3.8
At4g14060.1 68417.m02171 major latex protein-related / MLP-relat... 28 6.6
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 28 6.6
At2g01520.1 68415.m00076 major latex protein-related / MLP-relat... 28 6.6
At5g58910.1 68418.m07380 laccase, putative / diphenol oxidase, p... 27 8.7
At5g05310.3 68418.m00572 expressed protein 27 8.7
At5g05310.2 68418.m00571 expressed protein 27 8.7
At5g05310.1 68418.m00570 expressed protein 27 8.7
At3g55130.1 68416.m06122 ABC transporter family protein breast c... 27 8.7
At3g20690.1 68416.m02618 F-box protein-related ontains weak hit ... 27 8.7
At2g22660.1 68415.m02685 glycine-rich protein 27 8.7
At2g01530.1 68415.m00077 major latex protein-related / MLP-relat... 27 8.7
At1g66300.1 68414.m07529 F-box family protein contains F-box dom... 27 8.7
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 192 bits (469), Expect = 1e-49
Identities = 91/200 (45%), Positives = 131/200 (65%), Gaps = 2/200 (1%)
Frame = +3
Query: 3 MDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHG 182
MDR YQTF R++EN NVI+ATY D +G+V+V P KG+V F +GLHG
Sbjct: 160 MDRCFLELQVDGEEAYQTFSRVIENANVIMATYEDP--LLGDVQVYPEKGTVAFSAGLHG 217
Query: 183 WAFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTKKWSKQKDDDN--KRSFCMYVLD 356
WAFTL F++MYA KF + K+M RLWGENFF+P T+KWS + KR F + +
Sbjct: 218 WAFTLTNFAKMYASKFGVVESKMMERLWGENFFDPATRKWSGKNTGSPTCKRGFVQFCYE 277
Query: 357 PIYKVFDAIMKFKKEEIDDLLKKIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALLQMIA 536
PI ++ M +K+++ +L K+GV++K+++ + GK L+K VM++WLPA ALL+M+
Sbjct: 278 PIKQIIATCMNDQKDKLWPMLAKLGVSMKNDEKELMGKPLMKRVMQTWLPASTALLEMMI 337
Query: 537 IHLPSPVVAQKYRMGDVIRG 596
HLPSP AQ+YR+ ++ G
Sbjct: 338 FHLPSPHTAQRYRVENLYEG 357
Score = 48.0 bits (109), Expect = 6e-06
Identities = 20/31 (64%), Positives = 24/31 (77%)
Frame = +1
Query: 580 EMLYEGPHDDEAAIGIKSCDPEAPLMMYVSK 672
E LYEGP DD+ A I++CDP PLM+YVSK
Sbjct: 352 ENLYEGPLDDQYANAIRNCDPNGPLMLYVSK 382
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 100 bits (240), Expect = 8e-22
Identities = 54/158 (34%), Positives = 83/158 (52%), Gaps = 7/158 (4%)
Frame = +3
Query: 126 EVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTK--- 296
EV P KG+V F L GW F + +F+ YA K L LWG ++ P+TK
Sbjct: 201 EVTFQPQKGNVVFVCALDGWGFGIAEFANFYASKLGASATALQKSLWGPRYYIPKTKMIV 260
Query: 297 -KWSKQKDDDNKRSFCMYVLDPIYKVFDAIMK--FKKEEIDDLLKKIGVTI-KHEDSDKD 464
K + K F +VL+P+++V++A + K ++ ++K ++I E +KD
Sbjct: 261 GKKNLSAGSKAKPMFVQFVLEPLWQVYEAALDPGGDKAVLEKVIKSFNLSIPPRELQNKD 320
Query: 465 GKALLKVVMRSWLPAGEALLQMIAIHLPSPVVAQKYRM 578
K +L+ VM WLP +A+L M HLP P+ AQ YR+
Sbjct: 321 PKNVLQSVMSRWLPLSDAVLSMAVKHLPDPIAAQAYRI 358
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 97.9 bits (233), Expect = 5e-21
Identities = 46/158 (29%), Positives = 88/158 (55%), Gaps = 3/158 (1%)
Frame = +3
Query: 135 VDPSKGSVGFGSGLHGWAFTLKQFSEMYADKFKI--DLVKLMNRLWGENFFNPQTKKWSK 308
+DP+ G+V F SG GW+FTL+ F+ MYA + D+ K +RLWG+ +++P T+ ++
Sbjct: 299 IDPAAGNVCFASGTAGWSFTLQSFARMYAKLHGVAMDVDKFASRLWGDVYYHPDTRVFNT 358
Query: 309 QKD-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHEDSDKDGKALLKV 485
+R+F ++L+P+YK++ ++ K+ ++ L ++GVT+ + + + LL++
Sbjct: 359 SPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRL 418
Query: 486 VMRSWLPAGEALLQMIAIHLPSPVVAQKYRMGDVIRGT 599
S + M+ H+PSP A ++ GT
Sbjct: 419 ACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYTGT 456
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 97.9 bits (233), Expect = 5e-21
Identities = 50/187 (26%), Positives = 99/187 (52%), Gaps = 3/187 (1%)
Frame = +3
Query: 48 YQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADK 227
Y + +E +N I+ + G + +DP+ G+V F SG GW+FTL+ F++MYA
Sbjct: 286 YYKLRHTIEVINNHISAASTTAGDLP--LIDPAAGNVCFASGTAGWSFTLQSFAKMYAKL 343
Query: 228 FKI--DLVKLMNRLWGENFFNPQTKKWSKQKD-DDNKRSFCMYVLDPIYKVFDAIMKFKK 398
+ D+ K +RLWG+ +++ T+ + + +R+F ++L+P+YK++ ++ K
Sbjct: 344 HGVAMDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHK 403
Query: 399 EEIDDLLKKIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALLQMIAIHLPSPVVAQKYRM 578
+ ++ L ++GVT+ + + + LL++ S + M+ H+PSP A ++
Sbjct: 404 KSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKV 463
Query: 579 GDVIRGT 599
GT
Sbjct: 464 DHSYTGT 470
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 97.9 bits (233), Expect = 5e-21
Identities = 50/187 (26%), Positives = 99/187 (52%), Gaps = 3/187 (1%)
Frame = +3
Query: 48 YQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADK 227
Y + +E +N I+ + G + +DP+ G+V F SG GW+FTL+ F++MYA
Sbjct: 286 YYKLRHTIEVINNHISAASTTAGDLP--LIDPAAGNVCFASGTAGWSFTLQSFAKMYAKL 343
Query: 228 FKI--DLVKLMNRLWGENFFNPQTKKWSKQKD-DDNKRSFCMYVLDPIYKVFDAIMKFKK 398
+ D+ K +RLWG+ +++ T+ + + +R+F ++L+P+YK++ ++ K
Sbjct: 344 HGVAMDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHK 403
Query: 399 EEIDDLLKKIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALLQMIAIHLPSPVVAQKYRM 578
+ ++ L ++GVT+ + + + LL++ S + M+ H+PSP A ++
Sbjct: 404 KSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKV 463
Query: 579 GDVIRGT 599
GT
Sbjct: 464 DHSYTGT 470
>At2g27035.1 68415.m03248 plastocyanin-like domain-containing
protein low similarity to SP:P80728 Mavicyanin
{Cucurbita pepo}; contains Pfam profile PF02298:
Plastocyanin-like domain
Length = 163
Score = 30.3 bits (65), Expect = 1.2
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -2
Query: 172 PDPKPTEPLLGSTRTSPMGPPSSLYVAIMTLTFSTI 65
P P P+ P ++ T+P+ PP+++ AI+ F +
Sbjct: 126 PQPPPSAPTNFTSTTTPLIPPNAITAAILIFAFKAL 161
>At5g28760.1 68418.m03532 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 520
Score = 29.1 bits (62), Expect = 2.8
Identities = 21/90 (23%), Positives = 47/90 (52%), Gaps = 5/90 (5%)
Frame = +3
Query: 204 FSEMYADKFKIDLVKLMNRLWGENFFNPQTKKWSKQKDDDNKRSFCM-YVLD--PIYKVF 374
F ++ D ++ L+K ++W ++ F+ + S Q+ ++ S + +VLD + +
Sbjct: 337 FIDLVVDSWRTRLIKEKKKIWWKDMFDHDVRSRSDQQMEEQHISENVPHVLDISSLKEAI 396
Query: 375 DAIMKFKKEEID-DLLKKIGVTI-KHEDSD 458
DA K +K +D ++ K +G + E++D
Sbjct: 397 DADFKREKVNVDVNVEKDVGENVPPFEEND 426
>At1g75800.1 68414.m08805 pathogenesis-related thaumatin family
protein similar to receptor serine/threonine kinase PR5K
[Arabidopsis thaliana] GI:1235680; contains Pfam
profile: PF00314 Thaumatin family
Length = 330
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -2
Query: 172 PDPKPTEPLLGSTRTSPMGPPSSLYVAIMT 83
PDPKPT P G++ T+P G S+ + + T
Sbjct: 262 PDPKPTTP-TGTSSTTPAGDSSTTWSPVDT 290
>At1g66310.1 68414.m07530 F-box family protein contains F-box domain
Pfam:PF00646
Length = 442
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/38 (36%), Positives = 25/38 (65%)
Frame = +1
Query: 79 LTSL*PHITMMVVPWVRCVSTLARALLVSGLVFMGGLS 192
LTSL P + ++V+ WV+ + LA +L+SG + + L+
Sbjct: 161 LTSL-PCVKVIVLEWVKFANDLALEMLISGCLVLESLT 197
>At1g56120.1 68414.m06444 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1045
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 3/38 (7%)
Frame = +2
Query: 467 QSFAEGCDALLVA-CW*SSASDDC--HSFTITCSGPEI 571
Q++ +G + LL C+ + A D C ++F+I C GPEI
Sbjct: 415 QTYGQGSNVLLEKNCFKTFAIDWCLDYNFSINCGGPEI 452
>At1g18485.1 68414.m02307 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 970
Score = 28.7 bits (61), Expect = 3.8
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +3
Query: 156 VGFGSGLHGWAFTLKQFSEMYADKFKIDL 242
+G G G+HGWA L+ E+ + +D+
Sbjct: 308 IGLGKGVHGWAVKLRLDKELVLNNALMDM 336
>At4g14060.1 68417.m02171 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:294060] ; contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 6.6
Identities = 20/73 (27%), Positives = 40/73 (54%), Gaps = 5/73 (6%)
Frame = +3
Query: 285 PQTKKWSKQKDDDNKRSFCM----YVLDPIYKVFDAIMKFKKEEIDDLLKKIGVT-IKHE 449
P+ K ++ DD+N + +V++ + KV+D I++F ++ DD++ KI +T K
Sbjct: 66 PEMFKERREIDDENMAVTFVGLEGHVMEQL-KVYDTILQFIQKSPDDIVCKITMTWEKRA 124
Query: 450 DSDKDGKALLKVV 488
D + +K+V
Sbjct: 125 DDSPEPSNYMKLV 137
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 27.9 bits (59), Expect = 6.6
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -3
Query: 141 GRHAPHPWDHHHR 103
G HAPHP+D+H R
Sbjct: 375 GPHAPHPYDYHPR 387
>At2g01520.1 68415.m00076 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:169000] contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/62 (27%), Positives = 35/62 (56%), Gaps = 4/62 (6%)
Frame = +3
Query: 285 PQTKKWSKQKDDDNK----RSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHED 452
P+ K ++ DD+N R +V++ + KV+D I +F ++ DD++ KI + + ++
Sbjct: 66 PEVFKERREIDDENMAVTFRGLEGHVMEQL-KVYDVIFQFIQKSPDDIICKITMIWEKQN 124
Query: 453 SD 458
D
Sbjct: 125 DD 126
>At5g58910.1 68418.m07380 laccase, putative / diphenol oxidase,
putative similar to diphenol oxidase [Nicotiana
tabacum][GI:1685087]
Length = 523
Score = 27.5 bits (58), Expect = 8.7
Identities = 21/72 (29%), Positives = 36/72 (50%), Gaps = 4/72 (5%)
Frame = -3
Query: 666 HVHHQWGFRITALDTNGSFIIVGSLV*HLPYDISGPLQ---VMVNEWQSSEAE-LHQQAT 499
H H W R T +G+ +I+ L +PY P + ++++EW S+ E L +A+
Sbjct: 86 HAHILW-LRATV---HGAIVILPKL--GVPYPFPKPYKEKTIVLSEWWKSDVEELINEAS 139
Query: 498 KSASQPSAKLCH 463
+ + PSA H
Sbjct: 140 RIGTAPSASDAH 151
>At5g05310.3 68418.m00572 expressed protein
Length = 511
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = -2
Query: 490 ITTFSKALPSLSESSCLIVTPIFLRRSSISSFLNFMMASNT 368
++TF +LP+LS+S L++ F S + FL MAS T
Sbjct: 75 LSTFIFSLPNLSKSKALVIIHRFFSLSLVLCFL-LWMASPT 114
>At5g05310.2 68418.m00571 expressed protein
Length = 496
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = -2
Query: 490 ITTFSKALPSLSESSCLIVTPIFLRRSSISSFLNFMMASNT 368
++TF +LP+LS+S L++ F S + FL MAS T
Sbjct: 75 LSTFIFSLPNLSKSKALVIIHRFFSLSLVLCFL-LWMASPT 114
>At5g05310.1 68418.m00570 expressed protein
Length = 469
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = -2
Query: 490 ITTFSKALPSLSESSCLIVTPIFLRRSSISSFLNFMMASNT 368
++TF +LP+LS+S L++ F S + FL MAS T
Sbjct: 75 LSTFIFSLPNLSKSKALVIIHRFFSLSLVLCFL-LWMASPT 114
>At3g55130.1 68416.m06122 ABC transporter family protein breast
cancer resistance protein 1 BCRP1, Mus musculus,
EMBL:NP_036050
Length = 725
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = -2
Query: 514 SPAGNQERITTFSKALPSLSESSCLIVTPIFLRRSSI 404
+P G QER+T F+ +P++ CL P+F++ I
Sbjct: 463 TPRGAQERLTLFAFVVPTMF-YCCLDNVPVFIQERYI 498
>At3g20690.1 68416.m02618 F-box protein-related ontains weak hit to
TIGRFAM TIGR01640 : F-box protein interaction domain;
contains weak hit to Pfam PF00646: F-box domain
Length = 370
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/58 (27%), Positives = 23/58 (39%)
Frame = +3
Query: 261 LWGENFFNPQTKKWSKQKDDDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGV 434
+W N P WSK DN F + IY ++ F KEE + L + +
Sbjct: 277 IWVTNKIEPDEVSWSKFLKVDNTPRFFLTEGYLIYDEEKVVVFFNKEETEGLKSTVHI 334
>At2g22660.1 68415.m02685 glycine-rich protein
Length = 819
Score = 27.5 bits (58), Expect = 8.7
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = +3
Query: 408 DDLLKKIGVTIKHEDSDKD 464
DD++K IG ++H+D+D D
Sbjct: 240 DDMVKLIGKVLEHDDTDSD 258
>At2g01530.1 68415.m00077 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:20810]
Length = 151
Score = 27.5 bits (58), Expect = 8.7
Identities = 17/52 (32%), Positives = 30/52 (57%), Gaps = 4/52 (7%)
Frame = +3
Query: 285 PQTKKWSKQKDDDNK----RSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKI 428
P+ K K+ DD+N R +V++ + KV+D I +F ++ DD++ KI
Sbjct: 66 PEVFKERKEIDDENMVITFRGLEGHVMEQL-KVYDLIYQFSQKSPDDIVCKI 116
>At1g66300.1 68414.m07529 F-box family protein contains F-box domain
Pfam:PF00646
Length = 456
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/39 (33%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
Frame = +1
Query: 94 PHITMMVVPWVRCVSTLARALLVSGLVFMGGLS--PSNN 204
P + ++V+ WV+ + LA +L+SG + + L+ SNN
Sbjct: 175 PSVKVIVLDWVKFANDLALEMLISGCLVLKSLTLCRSNN 213
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,855,991
Number of Sequences: 28952
Number of extensions: 352860
Number of successful extensions: 1177
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 1106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1170
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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