Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30750
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 128 3e-30
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 125 2e-29
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 89 2e-18
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 53 2e-07
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 50 2e-06
At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein ... 47 1e-05
At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein ... 47 1e-05
At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein ... 47 1e-05
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 41 9e-04
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 128 bits (309), Expect = 3e-30
Identities = 67/181 (37%), Positives = 100/181 (55%), Gaps = 1/181 (0%)
Frame = +1
Query: 124 RVYASPMARRLAEIKNIRLGG-QGTGLYGSLKSGDLXXXXXXXXXXXXXXXXXXXXXXXX 300
RV+ASP+AR+LAE N+ L +GTG G + D+
Sbjct: 246 RVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADIDEYLASSGKGATAKPSKSTDSKAP 305
Query: 301 XXTFVDLPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVK 480
+VD+P S +R+ A RL +KQ+IPHY L+V V+K +A+R +N +
Sbjct: 306 ALDYVDIPHSQIRKVTASRLAFSKQTIPHYYLTVDTCVDKLMALRSQLNS-FKEASGGKR 364
Query: 481 VSVNDFIIKAVAAACKRVPTVNSHWMESFIRQFSNVDVSVAVATPTGLITPXLHNADSRG 660
+SVND ++KA A A ++VP NS W + +IRQF NV+++VAV T GL P + +AD +G
Sbjct: 365 ISVNDLVVKAAALALRKVPQCNSSWTDDYIRQFKNVNINVAVQTENGLYVPVVKDADRKG 424
Query: 661 I 663
+
Sbjct: 425 L 425
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 125 bits (302), Expect = 2e-29
Identities = 65/181 (35%), Positives = 99/181 (54%), Gaps = 1/181 (0%)
Frame = +1
Query: 124 RVYASPMARRLAEIKNIRLGG-QGTGLYGSLKSGDLXXXXXXXXXXXXXXXXXXXXXXXX 300
R++ASP+AR+LAE N+ L +GTG G + D+
Sbjct: 246 RIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADVEDFLASGSKETTAKPSKQVDSKVP 305
Query: 301 XXTFVDLPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVK 480
+VD+P + +R+ A RL +KQ+IPHY L+V V+K + +R +N +
Sbjct: 306 ALDYVDIPHTQIRKVTASRLAFSKQTIPHYYLTVDTCVDKMMGLRSQLNS-FQEASGGKR 364
Query: 481 VSVNDFIIKAVAAACKRVPTVNSHWMESFIRQFSNVDVSVAVATPTGLITPXLHNADSRG 660
+SVND +IKA A A ++VP NS W + +IRQF NV+++VAV T GL P + +AD +G
Sbjct: 365 ISVNDLVIKAAALALRKVPQCNSSWTDEYIRQFKNVNINVAVQTENGLYVPVVKDADKKG 424
Query: 661 I 663
+
Sbjct: 425 L 425
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 89.4 bits (212), Expect = 2e-18
Identities = 53/120 (44%), Positives = 70/120 (58%), Gaps = 2/120 (1%)
Frame = +1
Query: 310 FVDLPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVKVSV 489
+ D P S +R+ IAKRL +KQ IPH L V ++ LA RK + E VKVSV
Sbjct: 407 YEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDVVLDPLLAFRKEL-----QENHGVKVSV 461
Query: 490 NDFIIKAVAAACKRVPTVNSHW--MESFIRQFSNVDVSVAVATPTGLITPXLHNADSRGI 663
ND +IKAVA A + V N+ W + I +VD+S+AVAT GL+TP + NAD + I
Sbjct: 462 NDIVIKAVAVALRNVRQANAFWDAEKGDIVMCDSVDISIAVATEKGLMTPIIKNADQKSI 521
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 53.2 bits (122), Expect = 2e-07
Identities = 48/186 (25%), Positives = 85/186 (45%), Gaps = 3/186 (1%)
Frame = +1
Query: 124 RVYASPMARRLAEIKNIRLGGQ-GTGLYGSLKSGDLXXXXXXXXXXXXXXXXXXXXXXXX 300
R+ ASP A++LA+ + L G G+G G + + D+
Sbjct: 182 RIVASPYAKKLAKELKVELAGLVGSGPMGRIVAKDVEAVAAGGGVQAAVAVKEVVAAPGV 241
Query: 301 XXTFVDLPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVK 480
V +P + M+ +++ + + +P +++ T++ + A+ K + +
Sbjct: 242 ELGSV-VPFTTMQGAVSRNMVESL-GVPTFRVGYTISTDALDALYKKIKSK--------G 291
Query: 481 VSVNDFIIKAVAAACKRVPTVNSHWME--SFIRQFSNVDVSVAVATPTGLITPXLHNADS 654
V++ + KA A A + P VNS + SF+ S+++V+VAVA GLITP L NAD
Sbjct: 292 VTMTALLAKATALALAKHPVVNSSCRDGNSFVYN-SSINVAVAVAIDGGLITPVLQNADK 350
Query: 655 RGIIDL 672
I L
Sbjct: 351 VDIYSL 356
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 49.6 bits (113), Expect = 2e-06
Identities = 31/118 (26%), Positives = 56/118 (47%)
Frame = +1
Query: 319 LPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVKVSVNDF 498
+P++ +R+ +A RL ++ + V++ + +R + EK VK+ +
Sbjct: 238 VPMTRLRKRVATRLKDSQNTFALLTTFNEVDMTNLMKLRSQYKDAFL-EKHGVKLGLMSG 296
Query: 499 IIKAVAAACKRVPTVNSHWMESFIRQFSNVDVSVAVATPTGLITPXLHNADSRGIIDL 672
IKA +A + P VN+ I VD+S+AV T GL+ P + +AD D+
Sbjct: 297 FIKAAVSALQHQPVVNAVIDGDDIIYRDYVDISIAVGTSKGLVVPVIRDADKMNFADI 354
>At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 365
Score = 47.2 bits (107), Expect = 1e-05
Identities = 30/118 (25%), Positives = 55/118 (46%)
Frame = +1
Query: 319 LPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVKVSVNDF 498
+P++ +R+ +A RL ++ + V++ + +R + EK VK+ +
Sbjct: 139 VPMTRLRKRVATRLKDSQNTFALLTTFNEVDMTNLMKLRSQYKDAFY-EKHGVKLGLMSG 197
Query: 499 IIKAVAAACKRVPTVNSHWMESFIRQFSNVDVSVAVATPTGLITPXLHNADSRGIIDL 672
IKA +A + P VN+ I VD+S+AV T GL+ P + AD ++
Sbjct: 198 FIKAAVSALQHQPVVNAVIDGDDIIYRDYVDISIAVGTSKGLVVPVIRGADKMNFAEI 255
>At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 463
Score = 47.2 bits (107), Expect = 1e-05
Identities = 30/118 (25%), Positives = 55/118 (46%)
Frame = +1
Query: 319 LPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVKVSVNDF 498
+P++ +R+ +A RL ++ + V++ + +R + EK VK+ +
Sbjct: 237 VPMTRLRKRVATRLKDSQNTFALLTTFNEVDMTNLMKLRSQYKDAFY-EKHGVKLGLMSG 295
Query: 499 IIKAVAAACKRVPTVNSHWMESFIRQFSNVDVSVAVATPTGLITPXLHNADSRGIIDL 672
IKA +A + P VN+ I VD+S+AV T GL+ P + AD ++
Sbjct: 296 FIKAAVSALQHQPVVNAVIDGDDIIYRDYVDISIAVGTSKGLVVPVIRGADKMNFAEI 353
>At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 47.2 bits (107), Expect = 1e-05
Identities = 30/118 (25%), Positives = 55/118 (46%)
Frame = +1
Query: 319 LPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVKVSVNDF 498
+P++ +R+ +A RL ++ + V++ + +R + EK VK+ +
Sbjct: 238 VPMTRLRKRVATRLKDSQNTFALLTTFNEVDMTNLMKLRSQYKDAFY-EKHGVKLGLMSG 296
Query: 499 IIKAVAAACKRVPTVNSHWMESFIRQFSNVDVSVAVATPTGLITPXLHNADSRGIIDL 672
IKA +A + P VN+ I VD+S+AV T GL+ P + AD ++
Sbjct: 297 FIKAAVSALQHQPVVNAVIDGDDIIYRDYVDISIAVGTSKGLVVPVIRGADKMNFAEI 354
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 40.7 bits (91), Expect = 9e-04
Identities = 29/112 (25%), Positives = 57/112 (50%), Gaps = 1/112 (0%)
Frame = +1
Query: 319 LPLSGMRETIAKRLTAAKQSIPHYQLSVTVNVEKTLAMRKLVNERLASEKADVKVSVNDF 498
+P + M+ ++K + + S+P +++ VN + A+ + V + V++
Sbjct: 262 VPFTAMQSAVSKNMIESL-SVPTFRVGYPVNTDALDALYEKVKPK--------GVTMTAL 312
Query: 499 IIKAVAAACKRVPTVNSHWMESFIRQF-SNVDVSVAVATPTGLITPXLHNAD 651
+ KA A + P VN+ + + S+++++VAVA GLITP L +AD
Sbjct: 313 LAKAAGMALAQHPVVNASCKDGKSFSYNSSINIAVAVAINGGLITPVLQDAD 364
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,368,875
Number of Sequences: 28952
Number of extensions: 173711
Number of successful extensions: 437
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 423
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 431
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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