Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30737
(760 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g14920.1 68415.m01697 sulfotransferase family protein similar... 32 0.48
At4g26450.1 68417.m03805 expressed protein 31 0.83
At3g02120.1 68416.m00178 hydroxyproline-rich glycoprotein family... 31 1.1
At2g17200.1 68415.m01986 ubiquitin family protein weak similarit... 30 1.5
At5g40950.1 68418.m04975 50S ribosomal protein L27, chloroplast,... 29 2.5
At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) fa... 29 3.4
At5g13260.1 68418.m01523 expressed protein 29 4.4
At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger) fa... 29 4.4
At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger) fa... 29 4.4
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 28 5.9
At2g17190.1 68415.m01985 ubiquitin family protein contains INTER... 28 7.7
>At2g14920.1 68415.m01697 sulfotransferase family protein similar to
steroid sulfotransferase from [Brassica napus]
GI:3420008, GI:3420004; contains Pfam profile PF00685:
Sulfotransferase domain
Length = 333
Score = 31.9 bits (69), Expect = 0.48
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +1
Query: 517 QPAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPD 630
Q +K+ ++ + + L HHNPH P + +L L+ PD
Sbjct: 91 QRSKHSLEDHQHPLLHHNPHEIVPNLELDLYLKSSKPD 128
>At4g26450.1 68417.m03805 expressed protein
Length = 1248
Score = 31.1 bits (67), Expect = 0.83
Identities = 22/60 (36%), Positives = 28/60 (46%), Gaps = 9/60 (15%)
Frame = +1
Query: 562 HHNPHAEAPKPKPELKLEIRNPDP----LRRTNQPLNVK-----DLETRTSQSDKSQGAT 714
HH +EA P PE+ + N + L T L VK DLE+ T+QS S G T
Sbjct: 1012 HHEQVSEANSPHPEIVTLVNNVEQLENMLEETRSMLEVKESHIRDLESTTNQSKHSWGGT 1071
>At3g02120.1 68416.m00178 hydroxyproline-rich glycoprotein family
protein
Length = 126
Score = 30.7 bits (66), Expect = 1.1
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +1
Query: 556 LTHHNPHAEAPKPKPELKLEIRNPDPLRRTNQPL 657
+T NP + P+P PE + +P P TN+P+
Sbjct: 21 ITTSNPESSPPRPFPESSRKHDSPPPRASTNEPM 54
>At2g17200.1 68415.m01986 ubiquitin family protein weak similarity
to PLIC-2 (ubiquitin-like type II) [Homo sapiens]
GI:9937505; contains Pfam profiles PF00240: Ubiquitin
family, PF00627: UBA/TS-N domain
Length = 551
Score = 30.3 bits (65), Expect = 1.5
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Frame = +1
Query: 541 QYPNDLTHHNPHAEAP-KPKPELKLEIRNPDPLRRTNQPLNVKDLETRTSQSDKSQGATT 717
QY N L NP + P+L+ ++NPD LR+ + P ++ + T Q SQ T
Sbjct: 405 QYMNQLMSLNPQLRSMLDSNPQLREMMQNPDFLRQFSSPEMMQQM--MTLQQSLSQNRNT 462
Query: 718 PKDD 729
D
Sbjct: 463 ASQD 466
>At5g40950.1 68418.m04975 50S ribosomal protein L27, chloroplast,
putative (RPL27) identical to SP|Q9FLN4 ribosomal
protein L27, chloroplast precursor {Arabidopsis
thaliana}; similar to SP|P30155 50S ribosomal protein
L27, chloroplast precursor (CL27) {Nicotiana tabacum}
Length = 198
Score = 29.5 bits (63), Expect = 2.5
Identities = 12/47 (25%), Positives = 24/47 (51%)
Frame = +1
Query: 508 QKFQPAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPDPLRRTN 648
+KF P + ++ YP ++ NP++ + + +L+ R RR N
Sbjct: 127 EKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQ-REKKKARREN 172
>At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) family
protein contains Pfam profile: PF00097 zinc finger, C3HC4
type (RING finger)
Length = 4706
Score = 29.1 bits (62), Expect = 3.4
Identities = 15/66 (22%), Positives = 32/66 (48%)
Frame = +1
Query: 88 ENVHGALEITEREMSGFVCVLILAGVASAQITLDGIRCGQLICQLDEYCSPETNRCAPCN 267
E +++ E S ++C + ++T+ + CG ++C+ +CS +RC C
Sbjct: 4642 ERAEASMKEAETAKSQWLCQI--CQTKEVEVTI--VPCGHVLCR---HCSTSVSRCPFCR 4694
Query: 268 VVCNKT 285
+ N+T
Sbjct: 4695 LQVNRT 4700
>At5g13260.1 68418.m01523 expressed protein
Length = 537
Score = 28.7 bits (61), Expect = 4.4
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +1
Query: 568 NPHAEAPKPKPELKLEIRNPDPLRRTNQPLNVKDLETRTSQ 690
NP + KPKP + P + R++ P N + R SQ
Sbjct: 105 NPSSTIAKPKPVASSAVVPPPKISRSSSPANSPAVSVRASQ 145
>At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 341
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/38 (36%), Positives = 16/38 (42%)
Frame = +1
Query: 520 PAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPDP 633
P N V QYP HH P P P + L+ P P
Sbjct: 59 PNPNPVYQYPASYYHHPPPGAMPLPPYDHHLQHHPPHP 96
>At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 388
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/38 (36%), Positives = 16/38 (42%)
Frame = +1
Query: 520 PAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPDP 633
P N V QYP HH P P P + L+ P P
Sbjct: 59 PNPNPVYQYPASYYHHPPPGAMPLPPYDHHLQHHPPHP 96
>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
(CPK2) identical to calcium-dependent protein kinase
isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 646
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/70 (24%), Positives = 34/70 (48%)
Frame = +3
Query: 477 QRKSLLEIFKAKVPASQEPREAVP**LDASQPSCRSAETETRTQARNTKPGSPKTNEPTS 656
++K + E K +VP + RE V QP ET++ ++ TKP + +P +
Sbjct: 98 KQKVVPEESKQEVPPEESKREVVV------QPESAKPETKSESKPETTKPETTSETKPET 151
Query: 657 QREGLRDQNL 686
+ E + +++
Sbjct: 152 KAEPQKPKHM 161
>At2g17190.1 68415.m01985 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 538
Score = 27.9 bits (59), Expect = 7.7
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +1
Query: 541 QYPNDLTHHNPHAEAPKP-KPELKLEIRNPDPLRRTNQPLNVKDLETRTSQSDKSQGATT 717
QY N L NP + P+L+ ++NPD LR+ + P ++ + + QS SQ T
Sbjct: 389 QYMNQLMSLNPQLRSMLDMNPQLREMMQNPDFLRQFSSPEMMQQMMS-LQQSLFSQNRNT 447
Query: 718 PKDD 729
D
Sbjct: 448 AGQD 451
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,766,714
Number of Sequences: 28952
Number of extensions: 311446
Number of successful extensions: 950
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 943
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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