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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= maV30717
         (617 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protei...    96   1e-20
At5g28140.1 68418.m03398 hypothetical protein                          29   3.3  
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil...    27   7.5  
At5g38250.1 68418.m04611 serine/threonine protein kinase, putati...    27   10.0 
At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) / c...    27   10.0 
At2g22480.1 68415.m02667 phosphofructokinase family protein simi...    27   10.0 

>At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protein
           contains weak similarity to vacuolar ATP synthase
           subunit F (EC 3.6.3.14) (V-ATPase F subunit) (Vacuolar
           proton pump F subunit) (V-ATPase 14 kDa subunit)
           (Swiss-Prot:P50408) [Rattus norvegicus]; contains Pfam
           PF01990: ATP synthase (F/14-kDa) subunit
          Length = 128

 Score = 96.3 bits (229), Expect = 1e-20
 Identities = 41/94 (43%), Positives = 65/94 (69%)
 Frame = -2

Query: 616 IGEINKNRHPNFMVVDKNTPVSEIEECFKRFVKRXXXXXILINQNIAELIRHVIDAHSAP 437
           +G ++  R  N+++VD  T V +IE+ FK F  R     IL++Q IA +IR ++D+++ P
Sbjct: 32  VGNVDIRRKTNYLIVDSKTTVRQIEDAFKEFSARDDIAIILLSQYIANMIRFLVDSYNKP 91

Query: 436 VPSVLEIPSKDHPYDASKDSILRRAKGMFNPDDL 335
           VP++LEIPSKDHPYD + DS+L R K +F+ + +
Sbjct: 92  VPAILEIPSKDHPYDPAHDSVLSRVKYLFSAESV 125


>At5g28140.1 68418.m03398 hypothetical protein 
          Length = 212

 Score = 28.7 bits (61), Expect = 3.3
 Identities = 12/24 (50%), Positives = 16/24 (66%)
 Frame = -2

Query: 454 DAHSAPVPSVLEIPSKDHPYDASK 383
           DAHS P+ ++ EI S+ HP  A K
Sbjct: 137 DAHSRPMTNISEIASEKHPEPALK 160


>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
           LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
           HMM hit: AMP-binding enzymes PF00501
          Length = 691

 Score = 27.5 bits (58), Expect = 7.5
 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
 Frame = +2

Query: 500 DVNVIAFNKTLETFLDFTHRCILIDHH-EIRMSVFID 607
           D   + F KTLE   DF    + I H  E R+++F D
Sbjct: 103 DYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFAD 139


>At5g38250.1 68418.m04611 serine/threonine protein kinase, putative
           similar to receptor serine/threonine kinase PR55K
           gi|1235680|gb|AAC49208; contains protein kinase domain,
           Pfam:PF00069; contains serine/threonine protein kinase
           domain, INTERPRO:IPR002290
          Length = 579

 Score = 27.1 bits (57), Expect = 10.0
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +2

Query: 434 YWCRVRINHMSDQFSNVLIDEN 499
           Y C+ RI H   +  NVL+DEN
Sbjct: 380 YGCKTRIVHFDIKPQNVLLDEN 401


>At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) /
           cytochrome P450 identical to GA3 [Arabidopsis thaliana]
           GI:3342249; similar to ent-kaurene oxidase [Cucurbita
           maxima] GI:11934675; contains Pfam profile PF00067:
           Cytochrome P450
          Length = 509

 Score = 27.1 bits (57), Expect = 10.0
 Identities = 18/63 (28%), Positives = 28/63 (44%)
 Frame = -2

Query: 580 MVVDKNTPVSEIEECFKRFVKRXXXXXILINQNIAELIRHVIDAHSAPVPSVLEIPSKDH 401
           +  DK+   +   + F + VKR      L+  N  +  RH  DA    V S L   ++DH
Sbjct: 120 LTCDKSMVATSDYDDFHKLVKRCLLNG-LLGANAQKRKRHYRDALIENVSSKLHAHARDH 178

Query: 400 PYD 392
           P +
Sbjct: 179 PQE 181


>At2g22480.1 68415.m02667 phosphofructokinase family protein similar
           to phosphofructokinase [Amycolatopsis methanolica]
           GI:17432243; contains Pfam profile PF00365:
           Phosphofructokinase
          Length = 537

 Score = 27.1 bits (57), Expect = 10.0
 Identities = 12/29 (41%), Positives = 13/29 (44%)
 Frame = +1

Query: 319 ISISVPSRLG*TCP*HDVEWSPCWHHMGG 405
           IS S P+  G  C  HD  W   W H  G
Sbjct: 140 ISYSSPTSAGAECLDHDCSWVEQWIHRAG 168


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,913,031
Number of Sequences: 28952
Number of extensions: 236705
Number of successful extensions: 576
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 562
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 576
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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