Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30566
(860 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 181 1e-46
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 64 2e-11
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 64 2e-11
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 64 3e-11
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 60 4e-10
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 59 7e-10
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 54 2e-08
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 46 7e-06
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 46 7e-06
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 45 1e-05
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 39 8e-04
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 33 0.039
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 33 0.052
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 30 0.49
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 3.4
SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces pomb... 27 4.5
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 27 4.5
SPBC30D10.18c |rpl102|rpl1-2, rpl10a-2|60S ribosomal protein L10... 26 7.9
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 181 bits (440), Expect = 1e-46
Identities = 84/138 (60%), Positives = 97/138 (70%)
Frame = +2
Query: 170 QSQCANVRNTTVLSGLTPLSIILKRHYAEKQIFERTKPHCNVGTIGHVDHGKTTLTAAIT 349
+ C + + SGL + R YA++++F R KPH N+GTIGHVDHGKTTLTAAIT
Sbjct: 15 RKNCLRLNRISFASGLINRFTVPARTYADEKVFVRKKPHVNIGTIGHVDHGKTTLTAAIT 74
Query: 350 KVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTEQRHYGHTDCPGXADYIKNMI 529
K LSDL A Y+ ID APEEKARGITI+ AHVEY+T RHY H DCPG ADYIKNMI
Sbjct: 75 KCLSDLGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMI 134
Query: 530 TGTGQMDGAILXAXATDG 583
TG MDGAI+ ATDG
Sbjct: 135 TGAATMDGAIIVVSATDG 152
Score = 35.5 bits (78), Expect = 0.010
Identities = 17/28 (60%), Positives = 19/28 (67%)
Frame = +3
Query: 582 GVMPQTRGHLLLAXQIGXXHXGVVINQV 665
G MPQTR HLLLA Q+G V IN+V
Sbjct: 152 GQMPQTREHLLLARQVGVKQIVVYINKV 179
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 64.1 bits (149), Expect = 2e-11
Identities = 42/112 (37%), Positives = 57/112 (50%), Gaps = 15/112 (13%)
Frame = +2
Query: 272 RTKPHCNVGTIGHVDHGKTTLTAAIT---------------KVLSDLNLAQKKGYADIDN 406
+ K H NV IGHVD GK+T T + K ++L K +D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDK 62
Query: 407 APEEKARGITINVAHVEYQTEQRHYGHTDCPGXADYIKNMITGTGQMDGAIL 562
E+ RGITI++A +++T + + D PG D+IKNMITGT Q D AIL
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAIL 114
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 64.1 bits (149), Expect = 2e-11
Identities = 42/112 (37%), Positives = 57/112 (50%), Gaps = 15/112 (13%)
Frame = +2
Query: 272 RTKPHCNVGTIGHVDHGKTTLTAAIT---------------KVLSDLNLAQKKGYADIDN 406
+ K H NV IGHVD GK+T T + K ++L K +D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDK 62
Query: 407 APEEKARGITINVAHVEYQTEQRHYGHTDCPGXADYIKNMITGTGQMDGAIL 562
E+ RGITI++A +++T + + D PG D+IKNMITGT Q D AIL
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAIL 114
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 63.7 bits (148), Expect = 3e-11
Identities = 41/112 (36%), Positives = 57/112 (50%), Gaps = 15/112 (13%)
Frame = +2
Query: 272 RTKPHCNVGTIGHVDHGKTTLTAAIT---------------KVLSDLNLAQKKGYADIDN 406
+ K H NV IGHVD GK+T T + K ++L K +D
Sbjct: 3 KEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDK 62
Query: 407 APEEKARGITINVAHVEYQTEQRHYGHTDCPGXADYIKNMITGTGQMDGAIL 562
E+ RGITI++A +++T + + D PG D+IKNMITGT Q D A+L
Sbjct: 63 LKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVL 114
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 60.1 bits (139), Expect = 4e-10
Identities = 42/118 (35%), Positives = 64/118 (54%), Gaps = 17/118 (14%)
Frame = +2
Query: 260 QIFERT--KPHCNVGTIGHVDHGKTTLTAAITKVLSDLN--LAQK---------KG---Y 391
+IFE++ KP ++ GHVD GK+T+ I L ++N QK KG Y
Sbjct: 167 EIFEQSNPKPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSY 226
Query: 392 A-DIDNAPEEKARGITINVAHVEYQTEQRHYGHTDCPGXADYIKNMITGTGQMDGAIL 562
A +D EE+ARG+T++VA ++++++ Y D PG D+I MI G D A+L
Sbjct: 227 AWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVL 284
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 59.3 bits (137), Expect = 7e-10
Identities = 38/117 (32%), Positives = 54/117 (46%), Gaps = 15/117 (12%)
Frame = +2
Query: 278 KPHCNVGTIGHVDHGKTTLTAAIT------------KVLSDLNLAQKKGYA---DIDNAP 412
K H N+ IGHVD GK+TL I K+ + A K+ + +D+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 413 EEKARGITINVAHVEYQTEQRHYGHTDCPGXADYIKNMITGTGQMDGAILXAXATDG 583
EE+ +G T+ V ++TE R + D PG Y+ NMI G Q D +L A G
Sbjct: 296 EEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRG 352
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 54.4 bits (125), Expect = 2e-08
Identities = 36/106 (33%), Positives = 48/106 (45%), Gaps = 5/106 (4%)
Frame = +2
Query: 290 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYAD-----IDNAPEEKARGITINVAHV 454
NVG I H+D GKTTLT K+L G D +D P E+ RGITIN A +
Sbjct: 30 NVGIIAHIDAGKTTLTE---KMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAI 86
Query: 455 EYQTEQRHYGHTDCPGXADYIKNMITGTGQMDGAILXAXATDGGNA 592
+ + D PG AD+ + +DGA+ + G A
Sbjct: 87 SFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEA 132
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 46.0 bits (104), Expect = 7e-06
Identities = 26/90 (28%), Positives = 43/90 (47%), Gaps = 3/90 (3%)
Frame = +2
Query: 290 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTE 469
N+ I HVDHGK+TLT ++ + ++ A+ +D +E+ RG+TI + E
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAE 80
Query: 470 QRHYGHTDCPGXA---DYIKNMITGTGQMD 550
D A D++ N+I G +D
Sbjct: 81 MTDDDMKDMKEPADGTDFLVNLIDSPGHVD 110
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 46.0 bits (104), Expect = 7e-06
Identities = 26/90 (28%), Positives = 43/90 (47%), Gaps = 3/90 (3%)
Frame = +2
Query: 290 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEYQTE 469
N+ I HVDHGK+TLT ++ + ++ A+ +D +E+ RG+TI + E
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAE 80
Query: 470 QRHYGHTDCPGXA---DYIKNMITGTGQMD 550
D A D++ N+I G +D
Sbjct: 81 MTDDDMKDMKEPADGTDFLVNLIDSPGHVD 110
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 45.2 bits (102), Expect = 1e-05
Identities = 31/111 (27%), Positives = 48/111 (43%), Gaps = 4/111 (3%)
Frame = +2
Query: 290 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINV---AHVEY 460
N I H+DHGK+TL+ I K+ +N + +D E+ RGIT+ + + Y
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNFRNQF-LDKLEVERRRGITVKAQTCSMIYY 118
Query: 461 QTEQRH-YGHTDCPGXADYIKNMITGTGQMDGAILXAXATDGGNATNTRTF 610
Q + D PG D+ ++ +G IL A+ G A F
Sbjct: 119 YHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQGIQAQTLSNF 169
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 39.1 bits (87), Expect = 8e-04
Identities = 36/113 (31%), Positives = 50/113 (44%), Gaps = 22/113 (19%)
Frame = +2
Query: 290 NVGTIGHVDHGKTTLTAAITKVLS-------DLNLAQKKGYAD--IDNAPEEKA------ 424
N+GTIGHV HGK+T+ AI+ V + + N+ K GYA+ I E+
Sbjct: 25 NIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLGYANAKIYKCSNEECPRPGCY 84
Query: 425 RGITINVA-HVEYQ------TEQRHYGHTDCPGXADYIKNMITGTGQMDGAIL 562
R + N H + RH DCPG + M+ G MD A+L
Sbjct: 85 RSYSSNKEDHPPCEICNSPMNLVRHVSFVDCPGHDILMATMLNGAAVMDAALL 137
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 33.5 bits (73), Expect = 0.039
Identities = 46/182 (25%), Positives = 75/182 (41%), Gaps = 24/182 (13%)
Frame = +2
Query: 110 MASITFVKSLVSPAVKL-VLKQSQCANVRNTTVLSGLTPLSIILKRHYAEKQIFERTKPH 286
M ++F +SL S +L L A+V NT + + T + + + +R K
Sbjct: 1 MLKLSF-RSLTSRLPRLSTLVVRGYASVANTGIEASNTSENNLNIQEQLNDNDKKRLKQI 59
Query: 287 CNVGTIGHVDHGKTTLTAAI---TKVLSDLNLAQKKGY--ADIDNAPEEKARGITINVA- 448
N+G H+D GKTT T + T + D++ + K A +D E+ +GITI A
Sbjct: 60 RNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAKMDFMELEREKGITIQSAA 119
Query: 449 ----------HVEYQTEQR-------HYGHTDCPGXADYIKNMITGTGQMDGAILXAXAT 577
+E +Q+ + D PG D+ + +DGA+L A
Sbjct: 120 THCTWERTVDQIEANEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAV 179
Query: 578 DG 583
G
Sbjct: 180 SG 181
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 33.1 bits (72), Expect = 0.052
Identities = 26/110 (23%), Positives = 45/110 (40%), Gaps = 12/110 (10%)
Frame = +2
Query: 290 NVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADIDNAPEEKARGITINVAHVEY--- 460
N + HVDHGKTTL ++ ++ +D +E RGIT+ + +
Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRGITMKSSAISLFFK 80
Query: 461 ---QTEQRH------YGHTDCPGXADYIKNMITGTGQMDGAILXAXATDG 583
Q +++ D PG D+ + + + DGA + A +G
Sbjct: 81 VISQNDEKRVEKDYLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 29.9 bits (64), Expect = 0.49
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +2
Query: 293 VGTIGHVDHGKTTLTAAITK 352
V +GHVDHGKTTL A K
Sbjct: 174 VTLMGHVDHGKTTLLDAFRK 193
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.1 bits (57), Expect = 3.4
Identities = 22/106 (20%), Positives = 40/106 (37%), Gaps = 6/106 (5%)
Frame = +2
Query: 305 GHVDHGKTTLTAAITKVLS-DLNLAQKKG--YADIDNAPEEKARGIT---INVAHVEYQT 466
GH+ HGK+ L + D +++ Y D E+ I + +A + +
Sbjct: 147 GHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMSIKSTPLTLAVSDMKG 206
Query: 467 EQRHYGHTDCPGXADYIKNMITGTGQMDGAILXAXATDGGNATNTR 604
+ + D PG D++ + DG +L +G TR
Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTR 252
>SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.6 bits (56), Expect = 4.5
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1
Query: 317 DQHVRLCQHCNGALYVQRFVFLHN 246
D H+R+ G Y++RFV HN
Sbjct: 475 DYHIRITFLAKGTEYIERFVKAHN 498
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 26.6 bits (56), Expect = 4.5
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 278 KPHCNVGTIGHVDHGKTTLTAAITKVL 358
KP +G +G GK+TLT A+ +++
Sbjct: 1249 KPQEKIGIVGRTGAGKSTLTLALFRLI 1275
>SPBC30D10.18c |rpl102|rpl1-2, rpl10a-2|60S ribosomal protein
L10a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 216
Score = 25.8 bits (54), Expect = 7.9
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +2
Query: 266 FERTKPHCNVGTIGHVDHGKTTLTAAITKVLSDLNLAQKKGYADI 400
F+ K C +GHVD + L A ++ ++ L KKG+ +I
Sbjct: 156 FQLKKVLCLGVAVGHVDMAEEQLAANLSLAINFLVSLLKKGWQNI 200
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,867,126
Number of Sequences: 5004
Number of extensions: 52331
Number of successful extensions: 128
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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