BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30549
(463 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 30 0.20
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 0.60
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 27 1.1
SPAC1071.04c |||signal peptidase subunit |Schizosaccharomyces po... 27 1.8
SPAPB24D3.03 |||agmatinase |Schizosaccharomyces pombe|chr 1|||Ma... 27 1.8
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 25 4.3
SPBC11C11.05 |||KRE9 family cell wall biosynthesis protein |Schi... 25 4.3
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 25 4.3
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 7.4
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 7.4
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 24 9.8
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 24 9.8
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 29.9 bits (64), Expect = 0.20
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = -1
Query: 460 EMVHSQKLSIAFQXSSPPSIDATTPGSDNSGPI-FATRIRRCTSQ 329
E+++ +KL IAF + PPS P N G + R R C S+
Sbjct: 372 EVINLEKLQIAFTSNEPPSGSGVLPFLTNGGCYSYICRSRSCPSK 416
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 0.60
Identities = 26/85 (30%), Positives = 37/85 (43%), Gaps = 8/85 (9%)
Frame = -2
Query: 417 RHRQASTPRRQAATIPV--LSLRPGYGAAPPKRGRQRAYAPPCPA----SVHNGGTSS-- 262
R + A+ P+ A T P +S+ P A P G A PP PA SV N G S
Sbjct: 1693 RPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGDRSAL 1752
Query: 261 LAQLLSGAGTSRSIRAQSAPAPDGR 187
L Q+ +G +++ + GR
Sbjct: 1753 LQQIHTGTRLKKTVTTDKSKPIAGR 1777
>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 987
Score = 27.5 bits (58), Expect = 1.1
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = -1
Query: 439 LSIAFQXSSPPSIDATTPGSDNSGPIFATRIRRCT 335
L I F PP +DAT S G F T+ CT
Sbjct: 637 LVILFHELPPPGLDATAYSSWEKGDNFVTKKNVCT 671
>SPAC1071.04c |||signal peptidase subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 167
Score = 26.6 bits (56), Expect = 1.8
Identities = 18/70 (25%), Positives = 36/70 (51%)
Frame = -3
Query: 338 HLPSGADSERMLRRVQLVSTMVAPRRWLSSYPVQVLPEASVPSQHLHQTEGEAFSATIHP 159
H+ AD+ L+ L++T + + VQ+ E SVP H+ + +G+ SAT
Sbjct: 101 HIKISADTFLPLKNKPLITTKFTVLK--NRNAVQL--EWSVPVAHIFEEDGQISSATFEA 156
Query: 158 VIKVWVNRLD 129
I ++++++
Sbjct: 157 EISKYLSQIE 166
>SPAPB24D3.03 |||agmatinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 385
Score = 26.6 bits (56), Expect = 1.8
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +3
Query: 102 ANLTWGLRTVEAVYPDFDDWVNRGRERLTFRLVQVLTGHGCFGKYLHRIG 251
AN+ G+RT + + D+DD V+ G + + R + L G K R+G
Sbjct: 238 ANIHAGIRTPISSFSDYDDDVDCGFKIIEAREIDDLGIDGIVKKIRDRVG 287
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 25.4 bits (53), Expect = 4.3
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 368 SYLCDQDTALHLPSG-ADSERMLRRVQLVSTMVAPRRWLSS 249
+Y D T L SG + S+++ +V + P+RW SS
Sbjct: 75 AYTGDSTTDLDSKSGHSSSQKLSNKVSSALKLTIPKRWRSS 115
>SPBC11C11.05 |||KRE9 family cell wall biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 284
Score = 25.4 bits (53), Expect = 4.3
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = -2
Query: 309 YAPPCPASVHNGGTSSLAQLLSGAGTSRSIRAQSAPA 199
Y P P+S+ G +++Q+ AG + AQS A
Sbjct: 77 YTSPSPSSISQAGPFAISQVFGPAGRLYFLWAQSTYA 113
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.4 bits (53), Expect = 4.3
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = -2
Query: 354 PGYGAAPPKRGRQRAYAPP-CPASVHNGGTSSL 259
PGY A P G A APP P S + +S+L
Sbjct: 540 PGYPAVPAMPGIPGATAPPGAPGSYNTSESSNL 572
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 24.6 bits (51), Expect = 7.4
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = -2
Query: 429 PSXTRHRQASTPRRQAATIPVLSLRPGYG 343
P +R+ + AT P L L PGYG
Sbjct: 62 PPTSRYIMTINTFDENATFPALDLSPGYG 90
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 24.6 bits (51), Expect = 7.4
Identities = 9/20 (45%), Positives = 10/20 (50%)
Frame = +3
Query: 87 WSRRLANLTWGLRTVEAVYP 146
W LA TWG T+ YP
Sbjct: 1876 WCEHLAQNTWGDSTISLEYP 1895
>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 669
Score = 24.2 bits (50), Expect = 9.8
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 374 FRSYLCDQDTALHLPSGADSERMLRRVQLV 285
FR YL DQD ++ +GA +R ++ V
Sbjct: 349 FRGYLADQDCRWNVIAGAVDDRTEEEMKTV 378
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 24.2 bits (50), Expect = 9.8
Identities = 18/48 (37%), Positives = 22/48 (45%)
Frame = -2
Query: 333 PKRGRQRAYAPPCPASVHNGGTSSLAQLLSGAGTSRSIRAQSAPAPDG 190
P PP PAS + GTSS + LLS TS S ++ P G
Sbjct: 360 PPTSTSSTSTPPPPASTSSTGTSS-SPLLS---TSTSCTTSTSIPPTG 403
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,998,422
Number of Sequences: 5004
Number of extensions: 40454
Number of successful extensions: 118
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 174340060
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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