Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30460
(555 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 109 2e-24
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 107 6e-24
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 105 1e-23
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 74 6e-14
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 67 6e-12
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 46 2e-05
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 38 0.005
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 38 0.005
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 30 1.2
At3g15690.1 68416.m01988 biotin carboxyl carrier protein of acet... 29 2.1
At5g57550.1 68418.m07190 xyloglucan:xyloglucosyl transferase / x... 29 2.8
At3g15690.2 68416.m01989 biotin carboxyl carrier protein of acet... 29 2.8
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 28 4.8
At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP... 27 6.4
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam... 27 6.4
At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-contain... 27 6.4
At5g63530.1 68418.m07974 copper chaperone (CCH)-related low simi... 27 8.4
At5g16720.1 68418.m01958 expressed protein contains Pfam profile... 27 8.4
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 109 bits (261), Expect = 2e-24
Identities = 51/99 (51%), Positives = 71/99 (71%), Gaps = 3/99 (3%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKG 180
P+LSPTM G+I W KKEGDK++ G++LCE+ETDKAT+ E EEG+LAKI+ G K
Sbjct: 117 PSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKEEGAKE 176
Query: 181 VPVGKLLCIIVGDQNDVAAFKDF--KDDSSPATPQ-KPA 288
+ VG+++ I V D++D+ FKD+ D+ PA P+ KPA
Sbjct: 177 IQVGEVIAITVEDEDDIQKFKDYTPSSDTGPAAPEAKPA 215
Score = 33.9 bits (74), Expect = 0.073
Identities = 16/36 (44%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +2
Query: 419 RVYASPMARRLAEIKNIRLGG-QGTGLYGSLKSGDL 523
R++ASP+AR+LAE N+ L +GTG G + D+
Sbjct: 246 RIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADV 281
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 107 bits (256), Expect = 6e-24
Identities = 52/88 (59%), Positives = 62/88 (70%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKG 180
PALSPTM G+I W KKEGDK+ GD++ EIETDKAT+ FE+ EEGYLAKILIP G+K
Sbjct: 218 PALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYLAKILIPEGSKD 277
Query: 181 VPVGKLLCIIVGDQNDVAAFKDFKDDSS 264
V VGK + +IV D + A K SS
Sbjct: 278 VAVGKPIALIVEDAESIEAIKSSSAGSS 305
Score = 105 bits (251), Expect = 3e-23
Identities = 45/77 (58%), Positives = 62/77 (80%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKG 180
PALSPTM G++V W KKEGDK+ GD+LCEIETDKAT+ FE+ EEG+LAKIL+ G+K
Sbjct: 91 PALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKILVTEGSKD 150
Query: 181 VPVGKLLCIIVGDQNDV 231
+PV + + I+V +++D+
Sbjct: 151 IPVNEPIAIMVEEEDDI 167
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 105 bits (253), Expect = 1e-23
Identities = 49/95 (51%), Positives = 67/95 (70%), Gaps = 1/95 (1%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKG 180
P+LSPTM G+I W KKEGDK++ G++LCE+ETDKAT+ E EEGYLAKI+ G+K
Sbjct: 117 PSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKAEGSKE 176
Query: 181 VPVGKLLCIIVGDQNDVAAFKDFKDDS-SPATPQK 282
+ VG+++ I V D+ D+ FKD+ S + A P K
Sbjct: 177 IQVGEVIAITVEDEEDIGKFKDYTPSSTADAAPTK 211
Score = 34.7 bits (76), Expect = 0.042
Identities = 17/36 (47%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +2
Query: 419 RVYASPMARRLAEIKNIRLGG-QGTGLYGSLKSGDL 523
RV+ASP+AR+LAE N+ L +GTG G + D+
Sbjct: 246 RVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADI 281
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 74.1 bits (174), Expect = 6e-14
Identities = 38/82 (46%), Positives = 54/82 (65%), Gaps = 1/82 (1%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKG 180
PALS TM G IVSW K EGDKL++G+ + +E+DKA M ET +GYLA I++ G G
Sbjct: 45 PALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIMVEEG--G 102
Query: 181 V-PVGKLLCIIVGDQNDVAAFK 243
V PVG + ++ ++++A K
Sbjct: 103 VAPVGSAIALLAETEDEIADAK 124
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 67.3 bits (157), Expect = 6e-12
Identities = 34/81 (41%), Positives = 51/81 (62%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKG 180
PALS TM G IVSW K EG+KL++G+ + +E+DKA M ET +GYLA I++ G +
Sbjct: 61 PALSSTMTEGKIVSWIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVGEG-ET 119
Query: 181 VPVGKLLCIIVGDQNDVAAFK 243
PVG + ++ + ++ K
Sbjct: 120 APVGAAIGLLAETEAEIEEAK 140
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 45.6 bits (103), Expect = 2e-05
Identities = 26/101 (25%), Positives = 45/101 (44%), Gaps = 1/101 (0%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKG 180
P + ++ G++ ++ KK GD++ + + +IETDK T+ +P G + + L+ G
Sbjct: 99 PHMGESITDGTLAAFLKKPGDRVEADEAIAQIETDKVTIDIASPASGVIQEFLVKEGDTV 158
Query: 181 VPVGKLLCIIVGDQNDVAAFKDFKDDSSPA-TPQKPASQDK 300
P K+ I K PA P PA + K
Sbjct: 159 EPGNKVARISTSADAVSHVAPSEKAPEKPAPKPSPPAEKPK 199
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 37.9 bits (84), Expect = 0.005
Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 1/66 (1%)
Frame = +1
Query: 34 IVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVGK-LLCII 210
++ W KEGD + E LCE+++DKAT+ + +G +A I G + VG+ L+ +
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHSPGDI-IKVGETLVRLA 150
Query: 211 VGDQND 228
V D D
Sbjct: 151 VEDSQD 156
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 37.9 bits (84), Expect = 0.005
Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 1/66 (1%)
Frame = +1
Query: 34 IVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVGK-LLCII 210
++ W KEGD + E LCE+++DKAT+ + +G +A I G + VG+ L+ +
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHSPGDI-IKVGETLVRLA 150
Query: 211 VGDQND 228
V D D
Sbjct: 151 VEDSQD 156
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 29.9 bits (64), Expect = 1.2
Identities = 18/53 (33%), Positives = 27/53 (50%)
Frame = +1
Query: 52 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVGKLLCII 210
K GDK+ +G ++C IE K E + G + ++L G K V V L +I
Sbjct: 202 KVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDG-KPVSVDTPLFVI 253
>At3g15690.1 68416.m01988 biotin carboxyl carrier protein of
acetyl-CoA carboxylase-related contains weak similarity
to Biotin carboxyl carrier protein of acetyl-CoA
carboxylase, chloroplast precursor (BCCP)
(Swiss-Prot:Q42533) [Arabidopsis thaliana]
Length = 247
Score = 29.1 bits (62), Expect = 2.1
Identities = 16/42 (38%), Positives = 21/42 (50%)
Frame = +1
Query: 52 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTK 177
KE D++ EG +LC IE E+ G + KIL G K
Sbjct: 201 KEKDQVKEGQILCYIEQLGGQFPIESDVTGEVVKILREDGGK 242
>At5g57550.1 68418.m07190 xyloglucan:xyloglucosyl transferase /
xyloglucan endotransglycosylase / endo-xyloglucan
transferase (XTR3) identical to endoxyloglucan
transferase GI:5533317 from [Arabidopsis thaliana]
Length = 284
Score = 28.7 bits (61), Expect = 2.8
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 6/66 (9%)
Frame = +1
Query: 1 PALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKAT-MGFETPEEGYLAKI-----LI 162
P + T ++ ++W G L+ G+LL + D+A+ GF+T +E KI L+
Sbjct: 24 PVFAGTFDTEFDITWGDGRGKVLNNGELL-TLSLDRASGSGFQTKKEYLFGKIDMQLKLV 82
Query: 163 PAGTKG 180
P + G
Sbjct: 83 PGNSAG 88
>At3g15690.2 68416.m01989 biotin carboxyl carrier protein of
acetyl-CoA carboxylase-related contains weak similarity
to Biotin carboxyl carrier protein of acetyl-CoA
carboxylase, chloroplast precursor (BCCP)
(Swiss-Prot:Q42533) [Arabidopsis thaliana]
Length = 263
Score = 28.7 bits (61), Expect = 2.8
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = +1
Query: 52 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVG 192
KE D++ EG +LC IE E+ G + KIL G PVG
Sbjct: 201 KEKDQVKEGQILCYIEQLGGQFPIESDVTGEVVKIL---REDGEPVG 244
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 27.9 bits (59), Expect = 4.8
Identities = 11/46 (23%), Positives = 24/46 (52%)
Frame = -1
Query: 195 FPDRHSLCTGRNQYFGQVTFFRCLKAHSCFVSFNLAQKIAFTKFVP 58
+P+R S+ G+ ++ T+ RC + + +S N+A+ + P
Sbjct: 27 YPNRTSIIYGQTRFTWPQTYDRCCRLAASLISLNIAKNDVVSVVAP 72
>At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1)
identical to biotin carboxyl carrier protein of
acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
gi|9759121|dbj|BAB09606
Length = 280
Score = 27.5 bits (58), Expect = 6.4
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +1
Query: 52 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAG 171
K GDK+ +G +LC +E K E+ G + I+ G
Sbjct: 227 KVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDG 266
>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 580
Score = 27.5 bits (58), Expect = 6.4
Identities = 10/46 (21%), Positives = 24/46 (52%)
Frame = -1
Query: 195 FPDRHSLCTGRNQYFGQVTFFRCLKAHSCFVSFNLAQKIAFTKFVP 58
+P+R S+ G+ ++ T+ RC + + +S N+++ + P
Sbjct: 27 YPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNISKNDVVSVMAP 72
>At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-containing
protein similar to SP|Q06881 Biotin carboxyl carrier
protein of acetyl-CoA carboxylase (BCCP) {Anabaena sp.};
contains Pfam profile PF00364: Biotin-requiring enzyme
Length = 274
Score = 27.5 bits (58), Expect = 6.4
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = +1
Query: 52 KEGDKLSEGDLLCEIETDKATMGFETPEEGYLAKILIPAGTKGVPVG 192
KE D + EG +LC IE + E+ G + KIL G PVG
Sbjct: 212 KEKDIVKEGQVLCYIEQLGGQIPVESDVSGEIVKIL---REDGEPVG 255
>At5g63530.1 68418.m07974 copper chaperone (CCH)-related low
similarity to copper homeostasis factor [GI:3168840];
nearly identical to farnesylated protein ATFP3
[GI:4097547]; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 355
Score = 27.1 bits (57), Expect = 8.4
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 241 KDFKDDSSPATPQKPA 288
KD K+DS+PA P+ PA
Sbjct: 52 KDSKEDSAPAAPEAPA 67
>At5g16720.1 68418.m01958 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 675
Score = 27.1 bits (57), Expect = 8.4
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +1
Query: 43 WEKKEGDKLSEGDLLCEIETDKATMGFETPEEGY 144
W K GD+ G +L I+ DK GFE E Y
Sbjct: 149 WGKTLGDREDGGLILEMIDDDKFGDGFEIDRESY 182
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,199,968
Number of Sequences: 28952
Number of extensions: 178782
Number of successful extensions: 557
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 546
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 556
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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