Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30456
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 124 7e-29
At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alp... 122 2e-28
At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alp... 95 3e-20
At3g44210.1 68416.m04741 hypothetical protein 28 4.7
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 28 6.1
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai... 28 6.1
At2g34170.1 68415.m04182 expressed protein contains Pfam profile... 28 6.1
At4g15740.1 68417.m02396 C2 domain-containing protein similar to... 27 8.1
At3g63500.2 68416.m07153 expressed protein 27 8.1
At3g63500.1 68416.m07152 expressed protein 27 8.1
At1g35230.1 68414.m04369 arabinogalactan-protein (AGP5) identica... 27 8.1
>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial (PDHE1-A) identical to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}
Length = 389
Score = 124 bits (298), Expect = 7e-29
Identities = 59/118 (50%), Positives = 80/118 (67%)
Frame = +1
Query: 256 PYKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEA 435
P+ H D P+ S +S++ L + + ++RR+E A+ +LYK K+IRGFCHLY GQEA
Sbjct: 39 PFTAHLCDP-PSRSVESSSQELLDFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDGQEA 97
Query: 436 VAVGMRAAMRDADSVITAYRCHGWTYLMGVSVLGVLSELTGRRTGCSRGKGGSMHFVR 609
VA+GM AA+ D++ITAYR H G S+ V SEL GR+ GCS+GKGGSMHF +
Sbjct: 98 VAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYK 155
>At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial, putative similar to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 393
Score = 122 bits (295), Expect = 2e-28
Identities = 58/118 (49%), Positives = 79/118 (66%)
Frame = +1
Query: 256 PYKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEA 435
P+ H L + P+ S +SE+ L + + +RR+E A+ +LYK K+IRGFCHLY GQEA
Sbjct: 43 PFTSH-LCESPSRSVETSSEEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEA 101
Query: 436 VAVGMRAAMRDADSVITAYRCHGWTYLMGVSVLGVLSELTGRRTGCSRGKGGSMHFVR 609
+AVGM AA+ D++IT+YR H G ++ SEL GR+TGCS GKGGSMHF +
Sbjct: 102 LAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYK 159
>At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alpha
subunit, chloroplast identical to pyruvate dehydrogenase
E1 alpha subunit GB:AAB86803 GI:2454182 from
[Arabidopsis thaliana]; identical to cDNA pyruvate
dehydrogenase E1 alpha subunit mRNA, nuclear gene
encoding plastid protein GI:2454181
Length = 428
Score = 95.5 bits (227), Expect = 3e-20
Identities = 48/109 (44%), Positives = 65/109 (59%), Gaps = 1/109 (0%)
Frame = +1
Query: 292 TSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEAVAVGMRAAMRDA 471
TS +T E+ L+LYE + + R E +Y + GF HLY+GQEAV+ G + +
Sbjct: 75 TSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKS 134
Query: 472 DSVITAYRCHGWTYLMGVSVLGVLSELTGRRTGCSRGKGGSMH-FVRTH 615
DSV++ YR H GVS V+SEL G+ TGC RG+GGSMH F + H
Sbjct: 135 DSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEH 183
>At3g44210.1 68416.m04741 hypothetical protein
Length = 143
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/60 (28%), Positives = 32/60 (53%)
Frame = +1
Query: 460 MRDADSVITAYRCHGWTYLMGVSVLGVLSELTGRRTGCSRGKGGSMHFVRTHFLRWQRDC 639
+R ADS+ ++ H + L+G+ + GV+ + TG +RG + VR + + +DC
Sbjct: 9 LRVADSLSIGFKLHPYLTLIGMKLSGVIHDTTGYI--FNRGIKFLTNQVRAYDHGYSKDC 66
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 27.9 bits (59), Expect = 6.1
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -2
Query: 590 PPLPREQPVLRPVSSESTPSTLTPMR*VHP 501
PP+ P++ P S PST TP +HP
Sbjct: 600 PPVIPSPPIVGPTPSSPPPSTPTPGTLLHP 629
>At3g19840.1 68416.m02513 FF domain-containing protein / WW
domain-containing protein weak similarity to
transcription factor CA150b [Mus musculus] GI:6329166;
contains Pfam profiles PF01846: FF domain, PF00397: WW
domain
Length = 743
Score = 27.9 bits (59), Expect = 6.1
Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 6/64 (9%)
Frame = +1
Query: 142 IPSAAKFLAGNTITKVTAPVVATNAKYSTKKEAT------FEIKPYKLHKLDQGPATSAT 303
+PSA G+ +T ++AP ++ + + + T ++ KLH D G S+T
Sbjct: 255 VPSADLTEKGSDLTSLSAPAISNGGRDAASLKTTNFGSSALDLVKKKLH--DSGMPVSST 312
Query: 304 LTSE 315
+TSE
Sbjct: 313 ITSE 316
>At2g34170.1 68415.m04182 expressed protein contains Pfam profile:
PF05097 protein of unknown function (DUF688)
Length = 523
Score = 27.9 bits (59), Expect = 6.1
Identities = 20/63 (31%), Positives = 26/63 (41%)
Frame = -1
Query: 645 APTIPLPP*KVRPYKMHGTSLAPGAAGPAPRQLREHPQHTNAHEISPSVTTIRGDHRVCI 466
+P P PP K + + + P+PR L HPQ N E S SVT +
Sbjct: 443 SPLAPPPPKKPSESWLFSNLPSVSSKIPSPRYLF-HPQKKNVEENSTSVTKWETIVKTSY 501
Query: 465 AHR 457
HR
Sbjct: 502 THR 504
>At4g15740.1 68417.m02396 C2 domain-containing protein similar to
cold-regulated gene SRC2 [Glycine max] GI:2055230;
contains Pfam profile PF00168: C2 domain
Length = 468
Score = 27.5 bits (58), Expect = 8.1
Identities = 27/122 (22%), Positives = 50/122 (40%), Gaps = 8/122 (6%)
Frame = +1
Query: 61 LLSVVLQKDH*INLGFIEIIIDKMSKLIPSAAKFLAGNT--ITKVTAPVVATNAKYSTKK 234
L S L+ D+ + LG + + + + P F GN + +T P+ AT +T
Sbjct: 117 LFSYWLEGDNDLYLGEVNVSVQDLLASNP-IPPFTNGNINKMKSMTCPIKATEKSTNTTV 175
Query: 235 EATFEIKPYKLHKL-----DQGPATSATLTSE-DALKLYEQLTILRRIETASGNLYKEKI 396
++ KP + + D P+ + + D KL + L R + + L E +
Sbjct: 176 SLSYRFKPVPVEEFYPPSPDSLPSIGKPVYRDLDPAKLSQPLVFSPRFQATTTKLILELV 235
Query: 397 IR 402
I+
Sbjct: 236 IK 237
>At3g63500.2 68416.m07153 expressed protein
Length = 1162
Score = 27.5 bits (58), Expect = 8.1
Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +2
Query: 233 KRRHSKSSPTNSINWIRAL-QHQPHSLLK 316
K RH + SP+ W+R++ PH+ LK
Sbjct: 994 KPRHERGSPSQDTAWLRSVCSDNPHNQLK 1022
>At3g63500.1 68416.m07152 expressed protein
Length = 887
Score = 27.5 bits (58), Expect = 8.1
Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +2
Query: 233 KRRHSKSSPTNSINWIRAL-QHQPHSLLK 316
K RH + SP+ W+R++ PH+ LK
Sbjct: 719 KPRHERGSPSQDTAWLRSVCSDNPHNQLK 747
>At1g35230.1 68414.m04369 arabinogalactan-protein (AGP5) identical
to gi_3883128_gb_AAC77827
Length = 133
Score = 27.5 bits (58), Expect = 8.1
Identities = 14/42 (33%), Positives = 17/42 (40%)
Frame = -1
Query: 633 PLPP*KVRPYKMHGTSLAPGAAGPAPRQLREHPQHTNAHEIS 508
P PP P GT++ G AGPA L P +S
Sbjct: 76 PAPPTSTSPSGAPGTNVPSGEAGPAQSPLSGSPNAAAVSRVS 117
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,755,802
Number of Sequences: 28952
Number of extensions: 270635
Number of successful extensions: 767
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 767
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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