BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30453 (650 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) / isoch... 30 1.5 >At1g74710.2 68414.m08655 isochorismate synthase 1 (ICS1) / isochorismate mutase identical to GI:17223087 and GB:AF078080; contains Pfam profile PF00425: chorismate binding enzyme; contains TIGRfam profile TIGR00543: isochorismate synthases; identical to cDNA isochorismate synthase 1 precursor (ICS1) nuclear gene for plastid product GI:17223086 Length = 622 Score = 29.9 bits (64), Expect = 1.5 Identities = 14/48 (29%), Positives = 28/48 (58%), Gaps = 2/48 (4%) Frame = +1 Query: 187 ILVMLYES*NY--ILCNCCCSMPKKLYTRTQINYIYNRIVGQH**MIF 324 I+V+ Y++ N+ + C C CS P +L + Q ++Y R++ + +F Sbjct: 564 IMVLCYQNPNFYSLFCCCFCSSPSQLNMKQQ--HLYRRLIEERVTFVF 609 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,894,772 Number of Sequences: 28952 Number of extensions: 214185 Number of successful extensions: 388 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 382 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 387 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1354097952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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