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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= maV30328
         (758 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun...    40   5e-04
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S...    29   0.54 
SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo...    27   2.2  
SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor eIF...    27   3.8  
SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyce...    27   3.8  
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb...    25   8.9  

>SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit
           Cgs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 412

 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 28/74 (37%), Positives = 39/74 (52%), Gaps = 2/74 (2%)
 Frame = +1

Query: 538 RRKSVFAETYDPEEDDSDEGAPAVFPKSDAQRA-RLAEAVRGILLFRSLDAQQMQQVLDA 714
           RR+SV  E+ +P     +      FP  D +   RL  +V G  LF++LD +   +VL+A
Sbjct: 101 RRQSVSTESMNPSAFALE--TKRTFPPKDPEDLKRLKRSVAGNFLFKNLDEEHYNEVLNA 158

Query: 715 MFEKR-SEPGEYVI 753
           M EKR  E G  VI
Sbjct: 159 MTEKRIGEAGVAVI 172



 Score = 25.8 bits (54), Expect = 6.7
 Identities = 15/50 (30%), Positives = 23/50 (46%)
 Frame = +1

Query: 607 VFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIR 756
           VF  +  QR      +  + +  SLD  Q Q++ DA+     + G  VIR
Sbjct: 258 VFENAYRQRMLYESLLEEVPILSSLDKYQRQKIADALQTVVYQAGSIVIR 307


>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 29.5 bits (63), Expect = 0.54
 Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 6/70 (8%)
 Frame = +2

Query: 467 LSPAHLMSP*YQTKKSRQWRALTIGANP--FSPRLMTPK----RMILTKEPLPCSPSRTH 628
           L  A + SP Y T+       L +  +P   SP   +P     R   +++PLP SPSRT 
Sbjct: 37  LVDAFMQSPSYSTQPKSAVEPLGLSFSPGYISPSSQSPHHGPVRSPSSRKPLPASPSRTR 96

Query: 629 REPVSLRRSG 658
              + +  SG
Sbjct: 97  DHSLRVPVSG 106


>SPAC3G6.01 |hrp3||ATP-dependent DNA helicase
           Hrp3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1388

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 17/63 (26%), Positives = 31/63 (49%)
 Frame = +1

Query: 556 AETYDPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSE 735
           AE+   EE +SD+ +    PK  +++ +    V+  + F S +++ +    DA FE   E
Sbjct: 111 AESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEE 170

Query: 736 PGE 744
             E
Sbjct: 171 EEE 173


>SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor
           eIF3c|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 918

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 22/87 (25%), Positives = 44/87 (50%), Gaps = 3/87 (3%)
 Frame = +1

Query: 484 DESIISDEEEPPVARFNNRRKSVFAETYDPEEDDSDEGAPAVF-PKSDAQRARLA--EAV 654
           ++S +SDE E   +   +  +S      + E D+S+   P+ F  K + + A+ A  + +
Sbjct: 98  EDSEVSDESE---SESESESESEEESESEEESDESERSGPSSFLKKPEKEEAKPAGLKFL 154

Query: 655 RGILLFRSLDAQQMQQVLDAMFEKRSE 735
           RG     S D ++ ++V+ +  +KR E
Sbjct: 155 RGESSEESSDEEEGRRVVKSAKDKRYE 181


>SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 498

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 12/49 (24%), Positives = 25/49 (51%)
 Frame = +1

Query: 568 DPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDA 714
           D ++DD ++  PAV  +S     R+++    +   RS  ++Q+    D+
Sbjct: 426 DDDDDDPNDFRPAVERQSSTMNTRMSQPSSAVNTNRSYGSEQIPSYADS 474


>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1254

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 12/42 (28%), Positives = 25/42 (59%)
 Frame = +1

Query: 460 RGPVAGTPDESIISDEEEPPVARFNNRRKSVFAETYDPEEDD 585
           R PV+   + S +S+ ++P +A   +R+   +AE+ + E+ D
Sbjct: 171 RHPVSSKLENSELSEVDKPFIASRRSRKPVSYAESDEDEDFD 212


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,013,923
Number of Sequences: 5004
Number of extensions: 61903
Number of successful extensions: 163
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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