BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30328 (758 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun... 40 5e-04 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 29 0.54 SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharo... 27 2.2 SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor eIF... 27 3.8 SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyce... 27 3.8 SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 25 8.9 >SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit Cgs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 39.5 bits (88), Expect = 5e-04 Identities = 28/74 (37%), Positives = 39/74 (52%), Gaps = 2/74 (2%) Frame = +1 Query: 538 RRKSVFAETYDPEEDDSDEGAPAVFPKSDAQRA-RLAEAVRGILLFRSLDAQQMQQVLDA 714 RR+SV E+ +P + FP D + RL +V G LF++LD + +VL+A Sbjct: 101 RRQSVSTESMNPSAFALE--TKRTFPPKDPEDLKRLKRSVAGNFLFKNLDEEHYNEVLNA 158 Query: 715 MFEKR-SEPGEYVI 753 M EKR E G VI Sbjct: 159 MTEKRIGEAGVAVI 172 Score = 25.8 bits (54), Expect = 6.7 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = +1 Query: 607 VFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSEPGEYVIR 756 VF + QR + + + SLD Q Q++ DA+ + G VIR Sbjct: 258 VFENAYRQRMLYESLLEEVPILSSLDKYQRQKIADALQTVVYQAGSIVIR 307 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 29.5 bits (63), Expect = 0.54 Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 6/70 (8%) Frame = +2 Query: 467 LSPAHLMSP*YQTKKSRQWRALTIGANP--FSPRLMTPK----RMILTKEPLPCSPSRTH 628 L A + SP Y T+ L + +P SP +P R +++PLP SPSRT Sbjct: 37 LVDAFMQSPSYSTQPKSAVEPLGLSFSPGYISPSSQSPHHGPVRSPSSRKPLPASPSRTR 96 Query: 629 REPVSLRRSG 658 + + SG Sbjct: 97 DHSLRVPVSG 106 >SPAC3G6.01 |hrp3||ATP-dependent DNA helicase Hrp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1388 Score = 27.5 bits (58), Expect = 2.2 Identities = 17/63 (26%), Positives = 31/63 (49%) Frame = +1 Query: 556 AETYDPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDAMFEKRSE 735 AE+ EE +SD+ + PK +++ + V+ + F S +++ + DA FE E Sbjct: 111 AESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEE 170 Query: 736 PGE 744 E Sbjct: 171 EEE 173 >SPAC4A8.16c |tif33|SPAC823.01c|translation initiation factor eIF3c|Schizosaccharomyces pombe|chr 1|||Manual Length = 918 Score = 26.6 bits (56), Expect = 3.8 Identities = 22/87 (25%), Positives = 44/87 (50%), Gaps = 3/87 (3%) Frame = +1 Query: 484 DESIISDEEEPPVARFNNRRKSVFAETYDPEEDDSDEGAPAVF-PKSDAQRARLA--EAV 654 ++S +SDE E + + +S + E D+S+ P+ F K + + A+ A + + Sbjct: 98 EDSEVSDESE---SESESESESEEESESEEESDESERSGPSSFLKKPEKEEAKPAGLKFL 154 Query: 655 RGILLFRSLDAQQMQQVLDAMFEKRSE 735 RG S D ++ ++V+ + +KR E Sbjct: 155 RGESSEESSDEEEGRRVVKSAKDKRYE 181 >SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 26.6 bits (56), Expect = 3.8 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = +1 Query: 568 DPEEDDSDEGAPAVFPKSDAQRARLAEAVRGILLFRSLDAQQMQQVLDA 714 D ++DD ++ PAV +S R+++ + RS ++Q+ D+ Sbjct: 426 DDDDDDPNDFRPAVERQSSTMNTRMSQPSSAVNTNRSYGSEQIPSYADS 474 >SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 1254 Score = 25.4 bits (53), Expect = 8.9 Identities = 12/42 (28%), Positives = 25/42 (59%) Frame = +1 Query: 460 RGPVAGTPDESIISDEEEPPVARFNNRRKSVFAETYDPEEDD 585 R PV+ + S +S+ ++P +A +R+ +AE+ + E+ D Sbjct: 171 RHPVSSKLENSELSEVDKPFIASRRSRKPVSYAESDEDEDFD 212 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,013,923 Number of Sequences: 5004 Number of extensions: 61903 Number of successful extensions: 163 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 155 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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