Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30312
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 215 3e-56
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 206 9e-54
At5g44635.1 68418.m05469 minichromosome maintenance family prote... 30 1.9
At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putat... 29 4.4
At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putat... 29 4.4
At1g53840.1 68414.m06128 pectinesterase family protein contains ... 28 7.6
At1g15310.1 68414.m01832 signal recognition particle 54 kDa prot... 28 7.6
>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1025
Score = 215 bits (525), Expect = 3e-56
Identities = 107/186 (57%), Positives = 135/186 (72%), Gaps = 6/186 (3%)
Frame = +3
Query: 117 PNVTKMNADQKRLILARLTRSTGFENFLAKKWSSEKRFGLEGCEILIPAMKQVIDVSTKL 296
P + N++++ +I RLT ST FENFLA KW++ KRFGLEG E LIP MK++ D S L
Sbjct: 229 PTPRQYNSERRMVIYDRLTWSTQFENFLATKWTTAKRFGLEGAESLIPGMKEMFDRSADL 288
Query: 297 GVESIIMGMPHRGRLNVLANVCRKPLHQLFTQFAGLEAEDD------GSGDVKYHLGTYI 458
GVE+I++GMPHRGRLNVL NV RKPL Q+F++F+G D G+GDVKYHLGT
Sbjct: 289 GVENIVIGMPHRGRLNVLGNVVRKPLRQIFSEFSGGTRPVDEVGLYTGTGDVKYHLGTSY 348
Query: 459 ERLNRVTNKNIRLAVCANPSHLEAVDPVVXGKTRAEQFYRGDNEGKKVMSILLHGDAAFA 638
+R R K++ L++ ANPSHLEAVDPVV GKTRA+Q+Y D K M IL+HGD +FA
Sbjct: 349 DRPTR-GGKHLHLSLVANPSHLEAVDPVVIGKTRAKQYYTKDENRTKNMGILIHGDGSFA 407
Query: 639 GQGVVF 656
GQGVV+
Sbjct: 408 GQGVVY 413
Score = 51.2 bits (117), Expect = 7e-07
Identities = 20/36 (55%), Positives = 27/36 (75%)
Frame = +2
Query: 2 LREILNRLEQAYCNNIGIEFMFINSLEQCNWIRQRM 109
LR IL+RLEQAYC IG E+M I ++CNW+R ++
Sbjct: 191 LRSILSRLEQAYCGTIGYEYMHIADRDKCNWLRDKI 226
Score = 49.6 bits (113), Expect = 2e-06
Identities = 20/32 (62%), Positives = 24/32 (75%)
Frame = +1
Query: 655 FETMHLSDLPAYTTHGTIHXVANNQIGFTTRP 750
+ET+HLS LP Y T GT+H V NNQ+ FTT P
Sbjct: 413 YETLHLSALPNYCTGGTVHIVVNNQVAFTTDP 444
>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1017
Score = 206 bits (504), Expect = 9e-54
Identities = 104/185 (56%), Positives = 133/185 (71%), Gaps = 5/185 (2%)
Frame = +3
Query: 117 PNVTKMNADQKRLILARLTRSTGFENFLAKKWSSEKRFGLEGCEILIPAMKQVIDVSTKL 296
P + N +++ +IL RL ST FENFLA KW++ KRFGLEG E LIP MK++ D + L
Sbjct: 226 PTPWRYNRERREVILDRLAWSTQFENFLATKWTTAKRFGLEGGESLIPGMKEMFDRAADL 285
Query: 297 GVESIIMGMPHRGRLNVLANVCRKPLHQLFTQFA-GLEAEDD----GSGDVKYHLGTYIE 461
GVESI++GM HRGRLNVL NV RKPL Q+F++F+ G+ D+ G+GDVKYHLGT +
Sbjct: 286 GVESIVIGMSHRGRLNVLGNVVRKPLRQIFSEFSGGIRPVDEVGYTGTGDVKYHLGTSYD 345
Query: 462 RLNRVTNKNIRLAVCANPSHLEAVDPVVXGKTRAEQFYRGDNEGKKVMSILLHGDAAFAG 641
R R K I L++ ANPSHLEA D VV GKTRA+Q+Y D + K + IL+HGD +FAG
Sbjct: 346 RPTR-GGKKIHLSLVANPSHLEAADSVVVGKTRAKQYYSNDLDRTKNLGILIHGDGSFAG 404
Query: 642 QGVVF 656
QGVV+
Sbjct: 405 QGVVY 409
Score = 54.4 bits (125), Expect = 8e-08
Identities = 21/36 (58%), Positives = 28/36 (77%)
Frame = +2
Query: 2 LREILNRLEQAYCNNIGIEFMFINSLEQCNWIRQRM 109
LR IL RLEQAYC NIG E+M I ++CNW+R+++
Sbjct: 188 LRSILTRLEQAYCGNIGFEYMHIADRDKCNWLREKI 223
Score = 52.4 bits (120), Expect = 3e-07
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = +1
Query: 655 FETMHLSDLPAYTTHGTIHXVANNQIGFTTRP 750
+ET+HLS LP YTT GTIH V NNQ+ FTT P
Sbjct: 409 YETLHLSALPNYTTGGTIHIVVNNQVAFTTDP 440
>At5g44635.1 68418.m05469 minichromosome maintenance family protein
/ MCM family protein similar to SP|P97311 DNA
replication licensing factor MCM6 {Mus musculus};
contains Pfam profile PF00493: MCM2/3/5 family
Length = 831
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -2
Query: 560 SGLAXHHGVDGLQVRGVRANSQSNVLVGNAIQTLD 456
S A H GV GL+ GVR S + N++Q D
Sbjct: 272 SSTAGHEGVQGLKALGVRDLSYRLAFIANSVQIAD 306
>At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profiles PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase, PF01593: amine oxidase, flavin-containing
Length = 867
Score = 28.7 bits (61), Expect = 4.4
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +1
Query: 310 SLWACPIEGASTYSRTCAVSLCTSCSH 390
S+W+CP +G ++S +S C C+H
Sbjct: 173 SIWSCPSDGVLSFSAYSVLSFC--CNH 197
>At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profile PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase
Length = 867
Score = 28.7 bits (61), Expect = 4.4
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +1
Query: 310 SLWACPIEGASTYSRTCAVSLCTSCSH 390
S+W+CP +G ++S +S C C+H
Sbjct: 173 SIWSCPSDGVLSFSAYSVLSFC--CNH 197
>At1g53840.1 68414.m06128 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 586
Score = 27.9 bits (59), Expect = 7.6
Identities = 21/73 (28%), Positives = 37/73 (50%)
Frame = +3
Query: 390 QFAGLEAEDDGSGDVKYHLGTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVXGKTRAEQ 569
Q AGL+ + +GD + T E + +V K++++ V S V+ VV K++
Sbjct: 273 QTAGLKPDVTVAGDGTGDVLTVNEAVAKVPKKSLKMFVIYVKSGTY-VENVVMDKSKWNV 331
Query: 570 FYRGDNEGKKVMS 608
GD +GK ++S
Sbjct: 332 MIYGDGKGKTIIS 344
>At1g15310.1 68414.m01832 signal recognition particle 54 kDa protein
1 / SRP54 (SRP-54) (SRP-54A) identical to
Swiss-Prot:P37106 signal recognition particle 54 kDa
protein 1 (SRP54) [Arabidopsis thaliana]
Length = 479
Score = 27.9 bits (59), Expect = 7.6
Identities = 29/108 (26%), Positives = 52/108 (48%), Gaps = 6/108 (5%)
Frame = +3
Query: 186 FENFLAKKWSSEKRFGLEGCEILIPAMKQVI--DVSTKLGVESIIMG-MPHRGRLNVLAN 356
FE F K + S + G L+ +++V+ D+ +L VE++ G R +
Sbjct: 282 FEVFDVKPFVS-RLLGKGDWSGLVDKLQEVVPKDLQNEL-VENLSQGNFTLRSMYDQFQC 339
Query: 357 VCRKPLHQLFTQFAGLEAE--DDGSG-DVKYHLGTYIERLNRVTNKNI 491
R PL+QLF+ G+ AE G G + + + Y+ ++ +TNK +
Sbjct: 340 SLRIPLNQLFSMLPGISAEMMPKGHGEESRVKMKRYMTMMDSMTNKEL 387
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,783,407
Number of Sequences: 28952
Number of extensions: 355726
Number of successful extensions: 1054
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1009
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1048
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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