Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30300
(727 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g61530.1 68418.m07720 small G protein family protein / RhoGAP... 56 2e-08
At5g61530.2 68418.m07721 small G protein family protein / RhoGAP... 56 2e-08
At4g24580.1 68417.m03522 pleckstrin homology (PH) domain-contain... 41 0.001
At3g11490.1 68416.m01401 rac GTPase activating protein, putative... 41 0.001
At2g46710.1 68415.m05828 rac GTPase activating protein, putative... 41 0.001
At3g48030.1 68416.m05236 hypoxia-responsive family protein / zin... 29 2.4
At2g29860.1 68415.m03626 kelch repeat-containing F-box family pr... 29 2.4
At5g64750.1 68418.m08142 AP2 domain-containing transcription fac... 28 7.2
At4g11710.1 68417.m01869 hypothetical protein 27 9.6
At1g75340.1 68414.m08751 zinc finger (CCCH-type) family protein ... 27 9.6
>At5g61530.1 68418.m07720 small G protein family protein / RhoGAP
family protein contains Pfam domain, PF00620: RhoGAP
domain
Length = 376
Score = 56.4 bits (130), Expect = 2e-08
Identities = 38/148 (25%), Positives = 71/148 (47%), Gaps = 1/148 (0%)
Frame = +1
Query: 253 IFGVPLQQSVERSRCHDDTGLPLVVRDSIDYLQAHGLKSKDIYRTEPDKIKFQQLRKLFT 432
+FGV ++ +V+R +PL++ DYL GL S ++++ E D+ QQL +
Sbjct: 136 VFGVAIEITVQRQE--SSRPIPLILVKCADYLILTGLNSPNLFKAEGDRKLIQQLVSAYN 193
Query: 433 -DRGPTFPYHWDVPVACAMLKAFISELPESILTQELHGQFEQATAIAEPQREATMTNLIN 609
D + P + A+LK +++ LP + T EL+ + + A + R++ +
Sbjct: 194 QDPRASIPEGVNPVDVAALLKYYLASLPTPLTTFELYNEIKDARSSIHRMRQS-----LQ 248
Query: 610 KLPNCNHNLLAWLMRHFQHVVSNEQVNQ 693
KL N N+N L ++ V +N+
Sbjct: 249 KLSNVNYNTLEFITALLLRVSQKSLLNK 276
>At5g61530.2 68418.m07721 small G protein family protein / RhoGAP
family protein contains Pfam domain, PF00620: RhoGAP
domain
Length = 367
Score = 56.0 bits (129), Expect = 2e-08
Identities = 36/133 (27%), Positives = 67/133 (50%), Gaps = 1/133 (0%)
Frame = +1
Query: 253 IFGVPLQQSVERSRCHDDTGLPLVVRDSIDYLQAHGLKSKDIYRTEPDKIKFQQLRKLFT 432
+FGV ++ +V+R +PL++ DYL GL S ++++ E D+ QQL +
Sbjct: 136 VFGVAIEITVQRQE--SSRPIPLILVKCADYLILTGLNSPNLFKAEGDRKLIQQLVSAYN 193
Query: 433 -DRGPTFPYHWDVPVACAMLKAFISELPESILTQELHGQFEQATAIAEPQREATMTNLIN 609
D + P + A+LK +++ LP + T EL+ + + A + R++ +
Sbjct: 194 QDPRASIPEGVNPVDVAALLKYYLASLPTPLTTFELYNEIKDARSSIHRMRQS-----LQ 248
Query: 610 KLPNCNHNLLAWL 648
KL N N+N L ++
Sbjct: 249 KLSNVNYNTLEFI 261
>At4g24580.1 68417.m03522 pleckstrin homology (PH) domain-containing
protein-related / RhoGAP domain-containing protein
contains Pfam domain, PF00620: RhoGAP domain
Length = 902
Score = 40.7 bits (91), Expect = 0.001
Identities = 31/134 (23%), Positives = 52/134 (38%), Gaps = 1/134 (0%)
Frame = +1
Query: 301 DDTGLPLVVRDSIDYLQAHGLKSKDIYRTEPDKIKFQQLRKLFTDRGPTFPYHWDVPVAC 480
D G P + ++ +++ HG++ + I R D + + + F D +
Sbjct: 217 DVDGAPSFLEKALRFVENHGVRIEGILRQAADVDDVEHRIREYEKGKNEFSPEEDAHIIA 276
Query: 481 AMLKAFISELPESILTQELHGQFEQATAIAEPQR-EATMTNLINKLPNCNHNLLAWLMRH 657
LK F+ ELP S + +A R A + P N LL ++
Sbjct: 277 DCLKYFLRELPSSPVPASCCNALLEACRTDRGNRVNAMRAAICESFPEPNRRLLQRILMM 336
Query: 658 FQHVVSNEQVNQAN 699
Q V SN+ VN+ N
Sbjct: 337 MQTVASNKTVNRMN 350
>At3g11490.1 68416.m01401 rac GTPase activating protein, putative
similar to rac GTPase activating protein 1 GB:AAC62624
GI:3695059 [Lotus japonicus]; contains Pfam profile
PF00620: RhoGAP domain
Length = 435
Score = 40.7 bits (91), Expect = 0.001
Identities = 30/116 (25%), Positives = 55/116 (47%)
Frame = +1
Query: 358 GLKSKDIYRTEPDKIKFQQLRKLFTDRGPTFPYHWDVPVACAMLKAFISELPESILTQEL 537
GL+ + I+R + + + +R+ ++G P + DV +++KA+ ELP +L
Sbjct: 170 GLRVEGIFRINGENGQEEYIREEL-NKG-IIPDNIDVHCLASLIKAWFRELPSGVLDSLS 227
Query: 538 HGQFEQATAIAEPQREATMTNLINKLPNCNHNLLAWLMRHFQHVVSNEQVNQANIK 705
Q + E + E L+ LP+ +LL W + VV EQ+N+ N +
Sbjct: 228 PEQ------VMESESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNAR 277
>At2g46710.1 68415.m05828 rac GTPase activating protein, putative
similar to rac GTPase activating protein 2 [Lotus
japonicus] GI:3695061; contains Pfam profiles PF00620:
RhoGAP domain, PF00786: P21-Rho-binding domain
Length = 455
Score = 40.7 bits (91), Expect = 0.001
Identities = 41/158 (25%), Positives = 68/158 (43%), Gaps = 4/158 (2%)
Frame = +1
Query: 244 ALPIFGVPLQQSVERSRC-HDDTG--LPLVVRDSIDYLQAHG-LKSKDIYRTEPDKIKFQ 411
++ +FGV S + +C +DD G +P ++ L G LK++ I+R PD K +
Sbjct: 144 SVSVFGV----SAKSMQCSYDDRGNSVPTILLRMQKRLYTEGGLKAEGIFRINPDNGKEE 199
Query: 412 QLRKLFTDRGPTFPYHWDVPVACAMLKAFISELPESILTQELHGQFEQATAIAEPQREAT 591
+R+ P DV ++KA+ ELP +L Q + E
Sbjct: 200 HVRRQL--NCGVVPRGIDVHCLAGLIKAWFRELPTGVLDVLTPEQ------VMRCNTEED 251
Query: 592 MTNLINKLPNCNHNLLAWLMRHFQHVVSNEQVNQANIK 705
+ L+ LP +L W + VV +EQ N+ N +
Sbjct: 252 CSRLVILLPPVESAILDWAIGLMADVVEHEQFNKMNAR 289
>At3g48030.1 68416.m05236 hypoxia-responsive family protein / zinc
finger (C3HC4-type RING finger) family protein similar
to RING-H2 finger protein RHX1a [Arabidopsis thaliana]
GI:3790591; contains Pfam profiles PF00097: Zinc finger
C3HC4 type (RING finger), PF04588: Hypoxia induced
protein conserved region
Length = 349
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 266 HCNSLWSALVAMTTPVCRWSSETASITYRHTD 361
HC W L T P+CR S T+++ Y H++
Sbjct: 233 HCIDTW-LLSNSTCPLCRRSLSTSNVCYNHSE 263
>At2g29860.1 68415.m03626 kelch repeat-containing F-box family
protein contains Pfam profiles PF01344: Kelch motif,
PF00646: F-box domain
Length = 240
Score = 29.5 bits (63), Expect = 2.4
Identities = 15/58 (25%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +1
Query: 520 ILTQELHGQFEQATAIAEPQR--EATMTNLINKLPNCNHNLLAWLMRHFQHVVSNEQV 687
+L E+ G PQ E + +++ +P C + L+ L R F+HV++++Q+
Sbjct: 2 VLLSEIPGGSNGDDPNMNPQELPEELIESIVAPIPRCYYPSLSLLSRAFRHVITSQQL 59
>At5g64750.1 68418.m08142 AP2 domain-containing transcription
factor, putative contains similarity to transcription
factor
Length = 391
Score = 27.9 bits (59), Expect = 7.2
Identities = 15/35 (42%), Positives = 20/35 (57%)
Frame = -2
Query: 660 EMSHQPSQQIVVTVRQLIDEVRHRRFPLRFGYSGG 556
EM+ Q QQ +Q +D FPLRFG++GG
Sbjct: 303 EMARQ-QQQFQQHHQQSLDLYDQMSFPLRFGHTGG 336
>At4g11710.1 68417.m01869 hypothetical protein
Length = 473
Score = 27.5 bits (58), Expect = 9.6
Identities = 10/37 (27%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = +1
Query: 616 PNCNHNLLAWLMRHFQ-HVVSNEQVNQANIKR*WQLW 723
PN + L++W+ +H + H+++ +Q R +Q+W
Sbjct: 431 PNSSSRLISWIDKHIRNHLMAIKQSGDRRFDRGFQVW 467
>At1g75340.1 68414.m08751 zinc finger (CCCH-type) family protein
weak similarity to Nucleoporin NUP42 (Nuclear pore
protein NUP42) (Swiss-Prot:P49686) [Saccharomyces
cerevisiae]; contains Pfam profile PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 435
Score = 27.5 bits (58), Expect = 9.6
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +1
Query: 190 PSTVTSGVPFEEIFTLGVALPIFGVPLQQSVERSR 294
P VT + +EE+ + G+PLQ VER R
Sbjct: 138 PCDVTGDISYEELRAVAYEEAKRGIPLQSIVERER 172
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,832,221
Number of Sequences: 28952
Number of extensions: 255440
Number of successful extensions: 780
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 774
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 780
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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