Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30299
(722 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 312 2e-85
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 299 9e-82
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 293 6e-80
At5g14460.1 68418.m01692 pseudouridylate synthase TruB family pr... 33 0.25
At1g15870.1 68414.m01904 mitochondrial glycoprotein family prote... 31 0.58
At2g46980.2 68415.m05869 expressed protein 29 2.4
At2g46980.1 68415.m05868 expressed protein 29 2.4
At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains... 29 4.1
At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to S... 29 4.1
At4g40050.1 68417.m05671 expressed protein 28 5.5
At2g42100.1 68415.m05205 actin, putative very strong similarity ... 28 5.5
At2g16750.1 68415.m01921 protein kinase family protein contains ... 28 5.5
At5g63070.1 68418.m07914 40S ribosomal protein S15, putative 28 7.2
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea... 28 7.2
At4g08470.1 68417.m01398 mitogen-activated protein kinase, putat... 28 7.2
At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493 A... 28 7.2
At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496... 28 7.2
At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138... 28 7.2
At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671 A... 28 7.2
At5g06940.1 68418.m00784 leucine-rich repeat family protein cont... 27 9.5
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 312 bits (766), Expect = 2e-85
Identities = 144/215 (66%), Positives = 175/215 (81%), Gaps = 1/215 (0%)
Frame = +2
Query: 80 PVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 259
P+V++ GDEMTR+IW +IKEKLILP+LD+++ +DLG+ NRD TDD+VT++ AEA KYN
Sbjct: 81 PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYN 140
Query: 260 VGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPI 439
V IKCATITPDE RV+EF LK MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+KPI
Sbjct: 141 VAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPI 200
Query: 440 IIGRHAHADQYKATDFVVPGAGTLEIIFKPESGEA-IKHVVHEYKGAGVALAMFNTDASI 616
IGRHA DQY+ATD V+ G G L+++F PE G A ++ V+++KG GVALAM+N D SI
Sbjct: 201 CIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALAMYNVDESI 260
Query: 617 IDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFK 721
FA SS AL +K+PLYLSTKNTILKKYDGRFK
Sbjct: 261 RAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFK 295
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 299 bits (735), Expect = 9e-82
Identities = 144/221 (65%), Positives = 170/221 (76%), Gaps = 2/221 (0%)
Frame = +2
Query: 65 KIKA-GPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 241
KIK PVV++ GDEMTR+IW IK+KLI PFL++++ +DLG+ NRD TDD+VTI+ AE
Sbjct: 5 KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64
Query: 242 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 421
A KYNV IKCATITPDE RV EF LKKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV
Sbjct: 65 ATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVP 124
Query: 422 GWDKPIIIGRHAHADQYKATDFVVPGAGTLEIIFKPE-SGEAIKHVVHEYKGAGVALAMF 598
GW KPI IGRHA DQY+ATD +V G L+++F+P S + + V + G GVALAM+
Sbjct: 125 GWTKPICIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALAMY 184
Query: 599 NTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFK 721
NTD SI FA SS A +K+PLYLSTKNTILK YDGRFK
Sbjct: 185 NTDESIRAFAESSMYTAYQKKWPLYLSTKNTILKIYDGRFK 225
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 293 bits (720), Expect = 6e-80
Identities = 139/220 (63%), Positives = 170/220 (77%), Gaps = 1/220 (0%)
Frame = +2
Query: 65 KIK-AGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 241
KIK A P+V++ GDEMTR+IW IK+KLI PF+++++ +DLG+ +RD TDD+VTI+ AE
Sbjct: 5 KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64
Query: 242 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 421
A KKYNV IKCATITPDE RV EF LK+MW+SPNGTIRNIL GTVFRE IICKN+P+LV
Sbjct: 65 ATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVP 124
Query: 422 GWDKPIIIGRHAHADQYKATDFVVPGAGTLEIIFKPESGEAIKHVVHEYKGAGVALAMFN 601
GW KPI IGRHA DQY+ATD V+ G G L + F+ + G+ V GVA+AM+N
Sbjct: 125 GWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMYN 184
Query: 602 TDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFK 721
TD SI FA +S A ++K+PLYLSTKNTILKKYDGRFK
Sbjct: 185 TDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFK 224
>At5g14460.1 68418.m01692 pseudouridylate synthase TruB family
protein similar to SP|P09171 tRNA pseudouridine synthase
B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase)
(Pseudouridylate synthase) (Uracil hydrolyase)
{Escherichia coli O157:H7}; contains Pfam profile
PF01509: TruB family pseudouridylate synthase (N
terminal domain)
Length = 540
Score = 32.7 bits (71), Expect = 0.25
Identities = 27/101 (26%), Positives = 43/101 (42%)
Frame = +2
Query: 134 KEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYNVGIKCATITPDEKRVEEF 313
K+K++ V +GMEN D+ DD++ K+ + + EK+VE+F
Sbjct: 222 KKKVVAALGGKRSEVKRIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQF 281
Query: 314 KLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKP 436
K + KSPN + + G E + L WD P
Sbjct: 282 -FKGLTKSPNE--KGMASGGGDGEPFLVTRNGELPPRWDGP 319
>At1g15870.1 68414.m01904 mitochondrial glycoprotein family protein
/ MAM33 family protein similar to SUAPRGA1 [Emericella
nidulans] GI:6562379; contains Pfam profile PF02330:
Mitochondrial glycoprotein
Length = 242
Score = 31.5 bits (68), Expect = 0.58
Identities = 22/83 (26%), Positives = 37/83 (44%)
Frame = +2
Query: 8 PTNLKSTRLFIEVERYNMSKIKAGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDL 187
P ++ T+ F+ N GP + DE+ ++ ++E+ I L LH Y
Sbjct: 162 PDTIQITKFFVRKSSQNSPNAYIGPEFQEMEDELQDSVYRFLEERGISDDLAEFLHQY-- 219
Query: 188 GMENRDKTDDQVTIDCAEAIKKY 256
M+N+DK + I E +K Y
Sbjct: 220 -MKNKDKAE---YIRWMETVKSY 238
>At2g46980.2 68415.m05869 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 466 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 353
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At2g46980.1 68415.m05868 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 466 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 353
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains
Pfam PF01277: Oleosin domain
Length = 150
Score = 28.7 bits (61), Expect = 4.1
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = -3
Query: 153 GRISFSLIRSQIILVISSPRMSTTGPAFILDILYLSTSM 37
G +SFS + + + +I+SP + PAF++ + L ++M
Sbjct: 41 GLMSFSFVATVTLFLIASPLLLIFAPAFMVTVAVLVSAM 79
>At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to
SP|P53492 Actin 7 (Actin-2) {Arabidopsis thaliana}
Length = 377
Score = 28.7 bits (61), Expect = 4.1
Identities = 14/57 (24%), Positives = 26/57 (45%)
Frame = +2
Query: 530 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILK 700
+SG+ + H V Y+G + A+ D + D S K +R Y + + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDSLMKILTERGYMFTTTAEREIVR 212
>At4g40050.1 68417.m05671 expressed protein
Length = 599
Score = 28.3 bits (60), Expect = 5.5
Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 3/53 (5%)
Frame = -2
Query: 472 VLISMSMTSNDDGFVPPCYKS-RYILANNSLPENSTTKNITD--GPIRAFPHF 323
VL S S T +DD ++P C+ + +L++ S P S+ + + + G ++F HF
Sbjct: 545 VLTSFSRTEDDDPYLPQCHPNLPELLSSVSKPVQSSVQRLAEHLGVAKSF-HF 596
>At2g42100.1 68415.m05205 actin, putative very strong similarity to
SP|P53496 Actin 11 {Arabidopsis thaliana}, SP|P53493
Actin 3 {Arabidopsis thaliana}; contains Pfam profile
PF00022: Actin
Length = 378
Score = 28.3 bits (60), Expect = 5.5
Identities = 14/57 (24%), Positives = 26/57 (45%)
Frame = +2
Query: 530 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILK 700
+SG+ + H V Y+G + A+ D + D K ++R Y S + I++
Sbjct: 157 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTEYLTKIMMERGYTYTTSAEREIVR 213
>At2g16750.1 68415.m01921 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 617
Score = 28.3 bits (60), Expect = 5.5
Identities = 16/42 (38%), Positives = 21/42 (50%)
Frame = +2
Query: 440 IIGRHAHADQYKATDFVVPGAGTLEIIFKPESGEAIKHVVHE 565
+IG+ + YK F+ G G I KP EA+K VHE
Sbjct: 282 LIGKGGCNEVYKG--FLEDGKGVAVKILKPSVKEAVKEFVHE 321
>At5g63070.1 68418.m07914 40S ribosomal protein S15, putative
Length = 160
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +1
Query: 385 GYYLQEYTETCNRVGQTHHHWTSCSC 462
G+YL E++ TC +V HH C C
Sbjct: 124 GHYLAEFSMTCKKV--NHHRPRICGC 147
>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
family protein similar to katanin p80 subunit
[Strongylocentrotus purpuratus] GI:3005601; contains
Pfam profile PF00400: WD domain, G-beta repeat
Length = 871
Score = 27.9 bits (59), Expect = 7.2
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%)
Frame = +2
Query: 98 GDEMTRIIWDLIKEKLILPF---------LDIELHVYDLGMENRDKTDDQVTIDCAEAIK 250
G++ +WDL KL+ F LD H + L + DKT ++ E I
Sbjct: 255 GEDNVVKVWDLTAGKLLHEFKSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 314
Query: 251 K---YNVGIKCATITPDEKRV 304
G++C T PD K V
Sbjct: 315 SGGTETTGVRCLTFNPDGKSV 335
>At4g08470.1 68417.m01398 mitogen-activated protein kinase, putative
similar to mitogen-activated protein kinase [Arabidopsis
thaliana] gi|1255448|dbj|BAA09057; contains Pfam
PF00069: Protein kinase domain
Length = 560
Score = 27.9 bits (59), Expect = 7.2
Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 4/84 (4%)
Frame = +2
Query: 239 EAIKKYNVGIK--CATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNI-- 406
EAI +VG + C+ +TP + + +K S G + + +N
Sbjct: 237 EAISDSSVGSESTCSLMTPSMEFPDRISFRKRDFSEEGPSGRVKEKRKLMRNKLIENFRK 296
Query: 407 PRLVTGWDKPIIIGRHAHADQYKA 478
P +T W K ++GR ++A Y+A
Sbjct: 297 PEDITSWLKGQLLGRGSYASVYEA 320
>At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493
Actin 3 {Arabidopsis thaliana}; supported by full-length
cDNA: Ceres: 19581.
Length = 377
Score = 27.9 bits (59), Expect = 7.2
Identities = 13/57 (22%), Positives = 26/57 (45%)
Frame = +2
Query: 530 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILK 700
+SG+ + H V Y+G + A+ D + D + K +R Y + + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVR 212
>At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496
Actin 11 {Arabidopsis thaliana}
Length = 377
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/57 (24%), Positives = 25/57 (43%)
Frame = +2
Query: 530 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILK 700
+SG+ + H V Y+G + A+ D + D K +R Y S + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTSAEREIVR 212
>At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 512
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -3
Query: 381 LKTVPPRILRMVPLGLFH 328
LK +PPR+ R V LGL+H
Sbjct: 176 LKELPPRLKRRVRLGLYH 193
>At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671
Actin 1 (Actin 3) {Arabidopsis thaliana}
Length = 377
Score = 27.9 bits (59), Expect = 7.2
Identities = 13/57 (22%), Positives = 26/57 (45%)
Frame = +2
Query: 530 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILK 700
+SG+ + H V Y+G + A+ D + D + K +R Y + + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVR 212
>At5g06940.1 68418.m00784 leucine-rich repeat family protein
contains protein kinase domain, Pfam:PF00069; contains
leucine-rich repeats, Pfam:PF00560
Length = 872
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -2
Query: 481 SCFVLISMSMTSND-DGFVPPCYKSRYILANNSLPENSTTKNITDG 347
+C L+S+S+ N G +PP ++L L +NS T I G
Sbjct: 434 NCKKLVSLSLAGNAFTGEIPPSLADLHVLTYLDLSDNSLTGLIPQG 479
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,672,894
Number of Sequences: 28952
Number of extensions: 345243
Number of successful extensions: 884
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 864
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 882
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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