BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= maV30293 (758 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharo... 84 2e-17 SPCC777.09c |arg1||acetylornithine aminotransferase|Schizosaccha... 82 7e-17 SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces p... 56 4e-09 SPCC417.11c |||glutamate-1-semialdehyde 2,1-aminomutaseaminotran... 52 7e-08 SPBC1773.03c |||aminotransferase class-III, unknown specificty|S... 42 7e-05 SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch... 42 1e-04 SPAC27F1.05c |||aminotransferase class-III, unknown specificty|S... 36 0.005 SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 27 2.2 SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 27 3.8 SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 26 5.1 SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|... 26 6.7 SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha... 26 6.7 SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 25 8.9 SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 25 8.9 >SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 448 Score = 83.8 bits (198), Expect = 2e-17 Identities = 42/147 (28%), Positives = 70/147 (47%) Frame = +1 Query: 223 PVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTTNLYRHP 402 P ++ + +YD LD G ++ +GH HP + A ++ L L H + + P Sbjct: 33 PKIIVRAKGCCVYDEQDNAILDFTSGQMSAILGHSHPDITACIEKNLPKLVHLFSGFLSP 92 Query: 403 KIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLAKAYTGNLDIISLQTSYHGYTSSL 582 + + +L+ LP L+ +++G EANE A +AK YT + ++ +S+HG T Sbjct: 93 PVVQLATELSDLLPDGLDKTLFLSTGGEANEAALRMAKVYTNKYECVAFSSSWHGVTGGA 152 Query: 583 MGLTATQSYRMAIPVPPGFYHAVHPDP 663 LT + R P PG Y P+P Sbjct: 153 ASLTFAAARRGYGPALPGSYTIPEPNP 179 >SPCC777.09c |arg1||acetylornithine aminotransferase|Schizosaccharomyces pombe|chr 3|||Manual Length = 441 Score = 82.2 bits (194), Expect = 7e-17 Identities = 51/168 (30%), Positives = 81/168 (48%), Gaps = 13/168 (7%) Frame = +1 Query: 199 SITNAYKK-PVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLW 375 +I + Y + PV+ +G +L+D +G++Y+D G+ S+GH HP+V DQ L Sbjct: 46 NIISVYARYPVVAAKGEGSYLFDKEGRKYIDFTSGVAVTSLGHAHPEVARLAADQCSKLV 105 Query: 376 HTTNL-YRHP--KIYEYVEQLAAKLPG--DLNVVYLVNSGSEANELATLLA------KAY 522 H++NL Y P ++ + AK G ++ N G+EANE A A K Sbjct: 106 HSSNLFYNEPAIELSNVINNSLAKNSGIAGPTKIFFANCGTEANETALKFARKAAFEKYG 165 Query: 523 TGNLDIISLQTSYHGYTSSLMGLTATQSYRMAI-PVPPGFYHAVHPDP 663 G I+ S+HG + + +TA Y+ P+ P AV+ DP Sbjct: 166 EGKSQIVYFNNSFHGRSLGSLSITANPKYKRGFQPLLPDVVQAVYNDP 213 >SPBC21C3.08c |||ornithine aminotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 56.4 bits (130), Expect = 4e-09 Identities = 39/161 (24%), Positives = 75/161 (46%), Gaps = 8/161 (4%) Frame = +1 Query: 154 SYQQVEQMKGVYMPPSITNAYKKPVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHP 333 S +Q+E ++ Y + N + PV ++ ++D +G+ YLD V+ GHCHP Sbjct: 12 STEQIEVLENEY---AAHNYHPLPVCFSKAKGAKVWDPEGREYLDFLSAYSAVNQGHCHP 68 Query: 334 KVNAALKDQLDVLWHTTNLYRHPKIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLA 513 K+ AL +Q + ++ + + K + + + +V +N+G+EA E A LA Sbjct: 69 KIIEALVEQAQRVTLSSRAFYNDKFGPFAKYITEYF--GYEMVIPMNTGAEAVETACKLA 126 Query: 514 KAY--------TGNLDIISLQTSYHGYTSSLMGLTATQSYR 612 + + T I+S ++HG T ++ ++ R Sbjct: 127 RLWGYKAKKIPTDEAIILSCVDNFHGRTMGIISMSTDPDAR 167 >SPCC417.11c |||glutamate-1-semialdehyde 2,1-aminomutaseaminotransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 435 Score = 52.4 bits (120), Expect = 7e-08 Identities = 31/115 (26%), Positives = 52/115 (45%) Frame = +1 Query: 223 PVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTTNLYRHP 402 P+ + G+ L D DG Y D + GH +P + AL D + + Sbjct: 52 PIFIEAGYGSKLRDVDGHEYTDFLNELTAGIYGHSNPVIKKALMQGFDEIGISLGGTTTC 111 Query: 403 KIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLAKAYTGNLDIISLQTSYHG 567 ++ Y E L ++ + + NSG+EAN A + A+ +TG +I++ YHG Sbjct: 112 EL-NYAEALKSRFLS-IEKIRFCNSGTEANITAIIAARKFTGKRAVIAMHGGYHG 164 >SPBC1773.03c |||aminotransferase class-III, unknown specificty|Schizosaccharomyces pombe|chr 2|||Manual Length = 459 Score = 42.3 bits (95), Expect = 7e-05 Identities = 33/130 (25%), Positives = 55/130 (42%), Gaps = 7/130 (5%) Frame = +1 Query: 205 TNAYKKPVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTT 384 T+ +P + + +LY DG R +D GG +GH + +V A+ Q + + + Sbjct: 22 TSPNHRPPTVVRAEGVYLYLEDGTRIMDATGGAAVACLGHGNKEVIDAMHKQSEKVCYIH 81 Query: 385 NL-YRHPKIYEYVEQLAAKLPGDLNVVYLVNSGSEANELATLLAKAY------TGNLDII 543 ++ + + + L ++ P Y NSGSEA E L Y II Sbjct: 82 SMGFSNEPADKLANLLVSEHPDVFARAYFANSGSEAVETCLKLILQYWQLVGEKQRCHII 141 Query: 544 SLQTSYHGYT 573 + + YHG T Sbjct: 142 ARKQGYHGNT 151 >SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 41.9 bits (94), Expect = 1e-04 Identities = 36/113 (31%), Positives = 55/113 (48%), Gaps = 5/113 (4%) Frame = +1 Query: 208 NAYKKPVLLTQGHMQWLYDNDGKRYLDLFGGIVTVSVGHCHPKV-NAALKDQL-DVLWHT 381 +A K PV + +L D DG LD++ I T+ +G+ +P + AA D++ +L + Sbjct: 47 SAVKFPVDYEKSIGNYLVDLDGNVLLDVYSQIATIPIGYNNPTLLKAAKSDEVATILMNR 106 Query: 382 TNLYRHP-KIYEYV--EQLAAKLPGDLNVVYLVNSGSEANELATLLAKAYTGN 531 L +P K + V E P VY SGS+ANE+A LA + N Sbjct: 107 PALGNYPPKEWARVAYEGAIKYAPKGQKYVYFQMSGSDANEIAYKLAMLHHFN 159 >SPAC27F1.05c |||aminotransferase class-III, unknown specificty|Schizosaccharomyces pombe|chr 1|||Manual Length = 484 Score = 36.3 bits (80), Expect = 0.005 Identities = 30/123 (24%), Positives = 51/123 (41%), Gaps = 6/123 (4%) Frame = +1 Query: 262 DNDGKRYLDLFGGIVTVSVGHCHPKVNAALKDQLDVLWHTTNLYRHPKI-YEYVEQLAAK 438 D G +LDL GG+ V+VG+ + V L+ D + + + + +A Sbjct: 85 DEKGTEHLDLIGGVGVVTVGNNNQYVWDCLQKCFDAKLYMMGAISYRNLAAAFGRNMALL 144 Query: 439 LPGD-LNVVYLVNSGSEANE----LATLLAKAYTGNLDIISLQTSYHGYTSSLMGLTATQ 603 PG L + G+EANE L L + +S S+HG T+ + L + Sbjct: 145 SPGQKLTRTWTATGGAEANEGVIKLIRLATRYKPNKKKFLSTLNSFHGKTTGAVFLGGKE 204 Query: 604 SYR 612 ++ Sbjct: 205 KWQ 207 >SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 27.5 bits (58), Expect = 2.2 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 2/29 (6%) Frame = +1 Query: 49 EMANRGT--KLCFDIVRTYSTAKMPPTDF 129 E+ANR KLC+ I++ A +PPT+F Sbjct: 355 EIANRPRDFKLCYWILKEIGVATIPPTEF 383 >SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1055 Score = 26.6 bits (56), Expect = 3.8 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +1 Query: 145 TGPSYQQVEQMKGVYMPPSITNAYKKPVLL 234 +G SY+ V+ + VY+ PS + A KKP ++ Sbjct: 977 SGDSYRTVKSNQTVYIHPSSSVAEKKPKVI 1006 >SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 26.2 bits (55), Expect = 5.1 Identities = 9/34 (26%), Positives = 16/34 (47%) Frame = -1 Query: 341 FTFG*QWPTETVTIPPNRSRYLLPSLSYNHCMCP 240 +T+ +W E + P Y++ YN +CP Sbjct: 108 YTYNSRWKIEIPSYPVREIPYVVADTPYNKLLCP 141 >SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 885 Score = 25.8 bits (54), Expect = 6.7 Identities = 20/75 (26%), Positives = 30/75 (40%) Frame = +1 Query: 439 LPGDLNVVYLVNSGSEANELATLLAKAYTGNLDIISLQTSYHGYTSSLMGLTATQSYRMA 618 LP L Y + E N + L + Y +I HG+TSS + L + ++ Sbjct: 380 LPTTLLTSYQKSIQDEENSSVSFLNRWYRHFNQLIDFLKHPHGFTSSSV-LDDAFTTKII 438 Query: 619 IPVPPGFYHAVHPDP 663 IP G + DP Sbjct: 439 IPTSTGSIFCLSSDP 453 >SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 370 Score = 25.8 bits (54), Expect = 6.7 Identities = 21/50 (42%), Positives = 27/50 (54%), Gaps = 3/50 (6%) Frame = +1 Query: 391 YRHPKIYEYVEQLAAKLPGDLNVVY--LVNSGSEANE-LATLLAKAYTGN 531 Y HPK +EY +++A KL G L V LV S +A E L K +GN Sbjct: 316 YVHPKKFEYWKEVAEKL-GFLYVASGPLVRSSYKAGEYFMENLIKKRSGN 364 >SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60|Schizosaccharomyces pombe|chr 1|||Manual Length = 582 Score = 25.4 bits (53), Expect = 8.9 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = -1 Query: 158 YEGPVYGLGTKSVGGILAVLYVLTMSKHNFVPLLAISMCFSVEAIAIC 15 +E P+ L K V + +L L ++ PL+ I+ EA+A C Sbjct: 246 FENPLILLSEKKVSAVQDILPSLELAAQQRRPLVIIAEDVDGEALAAC 293 >SPBC4B4.04 |||translation initiation factor eIF2A |Schizosaccharomyces pombe|chr 2|||Manual Length = 576 Score = 25.4 bits (53), Expect = 8.9 Identities = 9/20 (45%), Positives = 11/20 (55%) Frame = +2 Query: 212 PIRSRCFSPRGTCSGYTTTT 271 P R+ C+SP G Y T T Sbjct: 36 PARNACYSPNGKLFAYATAT 55 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,195,077 Number of Sequences: 5004 Number of extensions: 68176 Number of successful extensions: 193 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 190 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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